FastQCFastQC Report
Thu 26 May 2016
SRR937195_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937195_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847699
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75400.8894666621052991No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58690.6923448063522548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39700.46832661121459385No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14560.1717590795789543No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.13790272254656427No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31300.049.1900671
GTATCAA56100.041.9294431
CGGGACG250.00161643237.9980962-63
TATAACG801.0776455E-535.6232072
TATCAAC72300.032.519112
ATCAACG73250.032.0973633
TCAACGC74200.031.6223984
CAACGCA75600.030.973975
AACGCAG77250.030.2509086
AGGACCG1606.240771E-826.7174055
ACGCAGA88400.026.1682137
CGCAGAG88050.026.0564428
TACCTGG5700.025.8320332
GGACCGA1858.965799E-925.6743856
TAACGCA1151.281803E-424.7813634
ATAACGC1151.281803E-424.7813633
GTACATG53050.024.6333831
GCAGAGT92550.024.3789259
GAGTACT56050.023.77139312-13
TACATGG54250.023.4642582
Produced by FastQC (version 0.11.5)