##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937195_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 847699 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30742869815819 33.0 31.0 34.0 31.0 34.0 2 32.49452458950642 34.0 31.0 34.0 31.0 34.0 3 32.55002188276735 34.0 31.0 34.0 31.0 34.0 4 36.16778361187167 37.0 35.0 37.0 35.0 37.0 5 36.1122084607862 37.0 35.0 37.0 35.0 37.0 6 36.165727457505554 37.0 36.0 37.0 35.0 37.0 7 36.10713708521539 37.0 35.0 37.0 35.0 37.0 8 36.08810320644474 37.0 35.0 37.0 35.0 37.0 9 37.78982752132538 39.0 38.0 39.0 35.0 39.0 10-11 37.71546681074297 39.0 37.0 39.0 35.0 39.0 12-13 37.6082029116467 39.0 37.0 39.0 35.0 39.0 14-15 38.8285700466793 40.0 38.0 41.0 35.0 41.0 16-17 38.800008611547256 40.0 38.0 41.0 35.0 41.0 18-19 38.818387776793415 40.0 38.0 41.0 35.0 41.0 20-21 38.75070514416084 40.0 38.0 41.0 35.0 41.0 22-23 38.688958580817015 40.0 38.0 41.0 34.5 41.0 24-25 38.70831037903784 40.0 38.0 41.0 35.0 41.0 26-27 38.5566580826449 40.0 38.0 41.0 34.0 41.0 28-29 38.39474860770156 40.0 38.0 41.0 34.0 41.0 30-31 38.38512431889149 40.0 38.0 41.0 34.0 41.0 32-33 38.2411563538473 40.0 37.5 41.0 34.0 41.0 34-35 38.090079733490306 40.0 37.0 41.0 34.0 41.0 36-37 37.916977016606126 40.0 37.0 41.0 33.5 41.0 38-39 37.795292904674895 40.0 37.0 41.0 33.0 41.0 40-41 37.567443160838934 40.0 37.0 41.0 33.0 41.0 42-43 37.32011716422929 40.0 36.5 41.0 33.0 41.0 44-45 36.93909394726194 40.0 36.0 41.0 31.5 41.0 46-47 36.442222416211415 39.5 36.0 41.0 30.5 41.0 48-49 36.00436711615798 39.0 35.5 41.0 29.5 41.0 50-51 35.71959563477131 39.0 35.0 41.0 28.5 41.0 52-53 35.47245779457095 39.0 35.0 40.0 28.0 41.0 54-55 35.24317121997312 39.0 35.0 40.0 27.0 41.0 56-57 34.94175585909621 38.0 35.0 40.0 26.0 41.0 58-59 34.677460395730094 38.0 34.0 40.0 26.0 41.0 60-61 34.33385140244356 38.0 34.0 40.0 25.5 41.0 62-63 33.96378726411144 37.0 34.0 40.0 24.5 41.0 64-65 33.542998163263135 37.0 33.0 39.5 23.0 41.0 66-67 33.11729104316508 36.0 33.0 39.0 22.0 40.5 68-69 32.6734996738229 35.5 32.0 39.0 20.5 40.0 70-71 32.23676387491315 35.0 32.0 38.0 19.5 40.0 72-73 31.778252658077925 35.0 31.0 37.5 18.0 39.5 74-75 31.313577696800397 35.0 31.0 37.0 15.5 39.0 76-77 29.9994897953165 33.5 29.5 35.5 13.0 38.0 78-79 30.641049476288163 34.0 31.0 36.0 11.0 38.0 80-81 30.46379788108751 34.0 31.0 36.0 7.5 37.0 82-83 30.07694181543213 34.0 30.5 35.0 6.0 37.0 84-85 29.665563484208427 34.0 30.0 35.0 2.0 36.5 86-87 29.31103434119894 34.0 29.5 35.0 2.0 36.0 88-89 28.895582040323276 34.0 29.0 35.0 2.0 36.0 90-91 28.360822650492686 34.0 29.0 35.0 2.0 35.0 92-93 27.883368978847443 33.0 27.5 35.0 2.0 35.0 94-95 27.435915932424127 33.0 27.0 35.0 2.0 35.0 96-97 26.807595620615338 33.0 25.5 35.0 2.0 35.0 98-99 25.870343128870037 32.5 24.5 34.0 2.0 35.0 100-101 22.966246273736317 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19284462038605454 1101 2 0.24885594410351075 1101 3 0.25438726708365067 1101 4 0.21246810591875231 1101 5 0.19607799050630348 1101 6 0.2349910146702996 1101 7 0.238474782462184 1101 8 0.15251512751311225 1101 9 0.2356159566592808 1101 10-11 0.24971531649946854 1101 12-13 0.325867724648468 1101 14-15 0.4620490399619541 1101 16-17 0.45616335136270436 1101 18-19 0.2869044800418621 1101 20-21 0.32730287297297167 1101 22-23 0.26159982642487734 1101 24-25 0.28938714135741606 1101 26-27 0.30724701992036074 1101 28-29 0.24004811354851796 1101 30-31 0.40204728251136856 1101 32-33 0.4529560914374713 1101 34-35 0.4504734182774044 1101 36-37 0.5850151359171747 1101 38-39 0.49717889846106544 1101 40-41 0.45911151776063264 1101 42-43 0.6186743450173893 1101 44-45 0.47404627176284464 1101 46-47 0.4687246041751365 1101 48-49 0.7004370869692664 1101 50-51 0.5552735077054436 1101 52-53 0.44292436105077826 1101 54-55 0.5181316105617384 1101 56-57 0.5668249355045489 1101 58-59 0.5724310991149366 1101 60-61 0.6221997031707218 1101 62-63 0.6107217573390429 1101 64-65 0.6744377134415203 1101 66-67 0.6184037858127596 1101 68-69 0.6967047808952458 1101 70-71 0.8293479684798584 1101 72-73 0.7386232289002912 1101 74-75 0.8772240244779823 1101 76-77 0.9193045090899297 1101 78-79 0.8639322223611288 1101 80-81 0.5863645789695475 1101 82-83 0.4398137025155542 1101 84-85 0.5988666181814075 1101 86-87 0.7655458774868613 1101 88-89 1.114138422057735 1101 90-91 1.146846441925483 1101 92-93 1.2925564122066433 1101 94-95 1.4750686446827537 1101 96-97 1.6342851810834595 1101 98-99 1.9034401900933169 1101 100-101 2.5144128265287797 1102 1 0.16602764797323033 1102 2 0.2151071694220974 1102 3 0.16944059761625851 1102 4 0.20113099160236203 1102 5 0.09339483455242004 1102 6 0.061930810683925586 1102 7 0.16797957870920754 1102 8 0.23825971277194213 1102 9 0.19673688976855885 1102 10-11 0.1162350908074572 1102 12-13 0.11994418452804467 1102 14-15 0.24075504518997803 1102 16-17 0.27137218065831803 1102 18-19 0.30488092475082595 1102 20-21 0.33117867234746967 1102 22-23 0.26054298914719 1102 24-25 0.07135336924333302 1102 26-27 0.11660787040815279 1102 28-29 0.09931006332212178 1102 30-31 0.32680907474401266 1102 32-33 0.416010548096196 1102 34-35 0.3921460656657203 1102 36-37 0.4301691467933324 1102 38-39 0.48297833131360335 1102 40-41 0.314893708630251 1102 42-43 0.3743662415771567 1102 44-45 0.40562535732509275 1102 46-47 0.48776517972477507 1102 48-49 0.7027848848967295 1102 50-51 0.7691441140630957 1102 52-53 0.5065760146473863 1102 54-55 0.686860167486536 1102 56-57 0.9002560025819903 1102 58-59 0.7321170966409625 1102 60-61 0.8734702418441032 1102 62-63 0.8261670439468887 1102 64-65 0.7592698739699273 1102 66-67 0.8084900832500921 1102 68-69 0.8917178801343866 1102 70-71 0.9257980454998886 1102 72-73 0.8895066252500108 1102 74-75 0.9722197650371953 1102 76-77 0.9469573238217599 1102 78-79 0.753793586315755 1102 80-81 0.9544415289765489 1102 82-83 0.9157697427526834 1102 84-85 1.1230819766746158 1102 86-87 1.0239881766513115 1102 88-89 1.080538436061378 1102 90-91 1.3463691511632163 1102 92-93 1.1372436350512487 1102 94-95 1.3099299794631314 1102 96-97 1.44417335617565 1102 98-99 1.9719004897712331 1102 100-101 2.4673700745799394 1103 1 0.16477613837020044 1103 2 0.11699758198199106 1103 3 0.18822947395217682 1103 4 0.1597857242890015 1103 5 0.09432656803119244 1103 6 0.019098665554267313 1103 7 0.040744392297582976 1103 8 6.595595391232223E-4 1103 9 0.07480641591553194 1103 10-11 0.047885784900202566 1103 12-13 0.05439089067163394 1103 14-15 0.10172466483494702 1103 16-17 0.1952979276683351 1103 18-19 0.1296606525677717 1103 20-21 0.24042393576508658 1103 22-23 0.15120877277724531 1103 24-25 0.13832536529563555 1103 26-27 0.16658709307622388 1103 28-29 0.12459266750096276 1103 30-31 0.025742288607688124 1103 32-33 0.07546358483311622 1103 34-35 0.12076083445297314 1103 36-37 0.17067163358024118 1103 38-39 0.3098122555023153 1103 40-41 0.28952263846780113 1103 42-43 0.2112114309755313 1103 44-45 0.3011583932828614 1103 46-47 0.4043766566035032 1103 48-49 0.6516823877211451 1103 50-51 0.5492533898432157 1103 52-53 0.5439232687931082 1103 54-55 0.5287779476883401 1103 56-57 0.4630287050635786 1103 58-59 0.47848106355305475 1103 60-61 0.6074351736035624 1103 62-63 0.646807537668245 1103 64-65 0.531012215575231 1103 66-67 0.5748595366764064 1103 68-69 0.5811763059917752 1103 70-71 0.7302192344827105 1103 72-73 0.2706416194713164 1103 74-75 0.33477203301283254 1103 76-77 0.6134787114878151 1103 78-79 0.6213113119770597 1103 80-81 0.42727597909147264 1103 82-83 0.19429068890043766 1103 84-85 0.27841282322102856 1103 86-87 0.3346661020347206 1103 88-89 0.6141003200459494 1103 90-91 0.9172945215840862 1103 92-93 0.79677621001197 1103 94-95 0.6634481661214835 1103 96-97 1.062135846598597 1103 98-99 1.419824055161392 1103 100-101 2.117577198607627 1104 1 0.25323393219313317 1104 2 0.27044180217330194 1104 3 0.2668055218223273 1104 4 0.15007986701822773 1104 5 0.17091098749194344 1104 6 0.1192113053768864 1104 7 0.20023811715793727 1104 8 0.14462000412886056 1104 9 0.18069495579074157 1104 10-11 0.21511328180882572 1104 12-13 0.20354360468730448 1104 14-15 0.2783728661510949 1104 16-17 0.3590094275901663 1104 18-19 0.34052186751440416 1104 20-21 0.2727554734940938 1104 22-23 0.17903471760150325 1104 24-25 0.16997200315683614 1104 26-27 0.17903787977832764 1104 28-29 0.2865870319565289 1104 30-31 0.32592919254048525 1104 32-33 0.3032305447660306 1104 34-35 0.3095897711614768 1104 36-37 0.4302140387424913 1104 38-39 0.41771196697380475 1104 40-41 0.39078477301021053 1104 42-43 0.3805433737814994 1104 44-45 0.288058239780014 1104 46-47 0.32520898316659697 1104 48-49 0.4205753194834472 1104 50-51 0.35000349288911536 1104 52-53 0.2945415441601611 1104 54-55 0.4812640187260442 1104 56-57 0.5409873658933506 1104 58-59 0.4040606078769997 1104 60-61 0.4786553435937577 1104 62-63 0.5193371011800494 1104 64-65 0.2926466300613555 1104 66-67 0.3210761533598969 1104 68-69 0.5197733894376242 1104 70-71 0.4762084395369328 1104 72-73 0.4689596617414544 1104 74-75 0.6456055321671457 1104 76-77 0.6282897821701887 1104 78-79 0.5680143763487635 1104 80-81 0.6341823636729664 1104 82-83 0.42599204768492527 1104 84-85 0.3594895106863838 1104 86-87 0.46889759286008115 1104 88-89 0.5896180368331905 1104 90-91 0.4694783838863472 1104 92-93 0.6216106259194092 1104 94-95 0.6195701582473596 1104 96-97 0.8890533885631484 1104 98-99 0.9486236060234248 1104 100-101 1.6227117267995617 1105 1 0.02309545063688745 1105 2 0.009217204464775364 1105 3 0.04277624297262861 1105 4 0.026097044547690018 1105 5 0.04115605433436542 1105 6 -0.054176743247460024 1105 7 -0.022376697139293356 1105 8 0.0338829151308957 1105 9 0.03266434745767555 1105 10-11 0.07983409974325895 1105 12-13 0.1302525290332781 1105 14-15 0.06239885906177989 1105 16-17 0.06385042779977823 1105 18-19 0.04295459546698055 1105 20-21 0.16392149084158802 1105 22-23 0.11811029231034098 1105 24-25 0.07270348717376152 1105 26-27 -0.032897632724285586 1105 28-29 0.05350914575954846 1105 30-31 -0.04715849481941348 1105 32-33 0.16688532099169606 1105 34-35 0.13344880437779238 1105 36-37 0.026822062099100208 1105 38-39 0.04923073176289705 1105 40-41 0.15083238135649424 1105 42-43 0.15362756747061468 1105 44-45 0.2739060403726157 1105 46-47 0.3864209281214528 1105 48-49 0.3883273217345007 1105 50-51 0.223362175794108 1105 52-53 0.14194366944509085 1105 54-55 0.10237211667724466 1105 56-57 0.3541803166099342 1105 58-59 0.33264006244889543 1105 60-61 0.3320953818164014 1105 62-63 0.7084268394191255 1105 64-65 0.4932966416754496 1105 66-67 0.41349129965027487 1105 68-69 0.41496751478297966 1105 70-71 0.5518416909735819 1105 72-73 0.4063710216480878 1105 74-75 0.3378183288222836 1105 76-77 0.34428409031950835 1105 78-79 0.44291405134295303 1105 80-81 0.48599029109525915 1105 82-83 0.49920616815802177 1105 84-85 0.5787933637331513 1105 86-87 0.584094305410293 1105 88-89 0.5691715645908779 1105 90-91 0.8643080343870722 1105 92-93 0.9584500624752934 1105 94-95 1.0641067180958252 1105 96-97 1.2324425136157906 1105 98-99 1.2391889008420272 1105 100-101 1.4055786708196223 1106 1 0.050458331658305156 1106 2 0.05327883389225718 1106 3 -0.10927495785905705 1106 4 0.055920879249569566 1106 5 0.003413309274549192 1106 6 -0.07718625406184998 1106 7 -0.06937847584838863 1106 8 -0.03361056699672815 1106 9 -0.13752318858352197 1106 10-11 -0.12717839995217872 1106 12-13 -0.3518954043341722 1106 14-15 -0.25194533333120006 1106 16-17 -0.274412934853828 1106 18-19 -0.08965669568089396 1106 20-21 -0.14624395347019714 1106 22-23 -0.06292412043138285 1106 24-25 0.05128994136997278 1106 26-27 0.1470565594250246 1106 28-29 0.15511485712136164 1106 30-31 -0.07417213158914393 1106 32-33 0.1631370367556073 1106 34-35 -0.2852503826140804 1106 36-37 -0.07178664626570708 1106 38-39 -0.0579669486055181 1106 40-41 -0.1706063425212534 1106 42-43 -0.1781422886894859 1106 44-45 -0.09819746587723444 1106 46-47 -0.21295936638078672 1106 48-49 -0.33540423126534336 1106 50-51 -0.26495438569318 1106 52-53 -0.47537803324246397 1106 54-55 -0.5145353660839049 1106 56-57 -0.37485487583989396 1106 58-59 -0.40887452052910334 1106 60-61 -0.5200443483721102 1106 62-63 -0.36342001291553316 1106 64-65 -0.3474682844553314 1106 66-67 -0.09714440610738251 1106 68-69 -0.12309198669115773 1106 70-71 0.1558068315240959 1106 72-73 0.27355668151667345 1106 74-75 0.22307128724597547 1106 76-77 -0.06905460350698789 1106 78-79 0.009465386186974456 1106 80-81 -0.18526528259933883 1106 82-83 0.06903471359632363 1106 84-85 0.3657462482470102 1106 86-87 0.47005793527636186 1106 88-89 0.2621533795239124 1106 90-91 0.20189321343566746 1106 92-93 0.29841903659060875 1106 94-95 0.29723537805375244 1106 96-97 0.343957015374194 1106 98-99 0.39580058184395384 1106 100-101 0.980771770402967 1107 1 -0.0659459427523359 1107 2 0.07694657654197101 1107 3 0.06788157242578308 1107 4 0.04850941478614601 1107 5 -0.05554434671385877 1107 6 -0.04825512863019554 1107 7 -0.03760784280522245 1107 8 -0.10788835707515432 1107 9 -0.1035617452901576 1107 10-11 -0.13582705070050594 1107 12-13 -0.06114303192750725 1107 14-15 -0.012960564123737583 1107 16-17 -0.010567907488123751 1107 18-19 0.11307288297657436 1107 20-21 0.026196896595259034 1107 22-23 -0.14478623145574687 1107 24-25 -0.07943423018134865 1107 26-27 -0.038377659943442666 1107 28-29 -0.023746931496525292 1107 30-31 0.02951845142275289 1107 32-33 0.028219638847751582 1107 34-35 -0.04842729054178818 1107 36-37 -0.03761350233646965 1107 38-39 -0.08606875136267433 1107 40-41 -0.019265636567610045 1107 42-43 0.10680482499569877 1107 44-45 -0.008413089772602689 1107 46-47 -0.0058462250152615525 1107 48-49 0.06158884282211119 1107 50-51 0.10422044795673457 1107 52-53 -0.009079220708237301 1107 54-55 0.28164936117187267 1107 56-57 0.20405507952816748 1107 58-59 0.2687908681649205 1107 60-61 0.32508789654804815 1107 62-63 0.29215470575568503 1107 64-65 0.48393643666307185 1107 66-67 0.3785624603736153 1107 68-69 0.2806308369680437 1107 70-71 0.3304326652167333 1107 72-73 0.39504177118839934 1107 74-75 0.47931451379673007 1107 76-77 0.3440451987762678 1107 78-79 0.26069771695270916 1107 80-81 0.416306351846103 1107 82-83 0.22418408878811746 1107 84-85 0.2784343025045253 1107 86-87 0.27443295227067566 1107 88-89 0.5359366835299113 1107 90-91 0.7849381289302109 1107 92-93 0.7113420979760257 1107 94-95 0.6436358845815136 1107 96-97 0.888641387205972 1107 98-99 0.9783482921753333 1107 100-101 1.503437333895679 1108 1 -0.011241893434970507 1108 2 0.03627863860585734 1108 3 -0.04808864435243265 1108 4 0.022958590081799457 1108 5 -0.0034011583644399934 1108 6 0.10357700742664377 1108 7 0.010236707155527824 1108 8 -0.02069082970833591 1108 9 0.024071170722905322 1108 10-11 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2316 82-83 1.1964557271097078 2316 84-85 0.9868002504634426 2316 86-87 1.0740724124096737 2316 88-89 1.1981914808448373 2316 90-91 1.432261357659545 2316 92-93 1.7684592022716679 2316 94-95 1.6987486718867153 2316 96-97 1.9559105349561534 2316 98-99 1.712343405911426 2316 100-101 2.3612612860767115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 21.0 6 30.0 7 91.0 8 207.0 9 378.0 10 659.0 11 1183.0 12 1912.0 13 2776.0 14 3806.0 15 5446.0 16 8061.0 17 12271.0 18 10751.0 19 5748.0 20 4527.0 21 4478.0 22 4621.0 23 4873.0 24 5486.0 25 6277.0 26 7655.0 27 9236.0 28 11607.0 29 14575.0 30 18362.0 31 23290.0 32 30107.0 33 40435.0 34 57098.0 35 83860.0 36 128373.0 37 189110.0 38 139560.0 39 10822.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.218057969885685 19.460543256731704 15.22520832079455 21.09619045258806 2 15.700620149369056 20.79275780672149 40.105627115285024 23.40099492862443 3 19.461506973583784 25.86047641910631 30.31347211687167 24.364544490438234 4 12.596806177664476 17.272168540956166 37.3314112674428 32.79961401393655 5 12.993044643573374 38.45476894909944 33.4606201308966 15.09156627643059 6 30.921942812248215 37.04498884627681 17.21011821413025 14.82295012734473 7 28.36160004907402 34.21898574847912 19.946702780114165 17.47271142233269 8 27.71844723185942 34.0961827252362 19.03989505708984 19.14547498581454 9 27.403830840899897 16.406177192612002 20.19655561702916 35.993436349458946 10-11 24.948832073648784 27.635988717693426 26.351039696873535 21.06413951178425 12-13 26.521367002083995 24.931914745523066 27.6769140111402 20.869804241252734 14-15 23.088148033677047 25.620827675861364 27.12136029416102 24.169663996300574 16-17 21.363538237039325 29.27979152977649 27.593992679005165 21.762677554179017 18-19 21.26651087237333 26.45726844080269 31.620008989039743 20.656211697784236 20-21 22.124198639020833 25.463416533118792 32.03515164884685 20.37723317901353 22-23 21.669599130347077 25.778508649047506 31.879822270877067 20.672069949728353 24-25 21.355352168253216 25.835659730434816 32.141852321314715 20.66713577999725 26-27 21.262004038063733 26.164659333347885 31.894628124765905 20.678708503822477 28-29 21.024921107735867 26.28413012062406 32.06789158580824 20.623057185831833 30-31 21.069590821259457 26.009203749448506 32.17242461348263 20.748780815809404 32-33 20.884619286585554 26.168635528218775 31.858279717540917 21.088465467654753 34-35 21.32413745916888 25.971836701470686 31.923005689519513 20.781020149840923 36-37 21.36737214506564 25.894155826537485 31.60302182732314 21.13545020107373 38-39 21.265815617227116 26.10421040027793 31.808125176581058 20.821848805913895 40-41 21.203575797541344 26.13244795617312 31.774485990900068 20.88949025538546 42-43 21.287641496634755 26.023111362791767 31.478938458180707 21.210308682392775 44-45 21.364010102642567 26.36348515215896 31.093347992624743 21.179156752573732 46-47 21.381764045964427 26.85699758994643 30.457568075460745 21.303670288628393 48-49 21.70976962341586 27.047631293654938 29.835708193592303 21.4068908893369 50-51 21.829328570636513 27.170257367296646 29.63569616101942 21.364717901047424 52-53 22.030225351215467 27.38094535914281 29.116408064655026 21.4724212249867 54-55 21.458619156091963 27.370269399869528 29.418225101126698 21.75288634291181 56-57 21.6933133105029 27.649024004982902 29.188662485150978 21.469000199363215 58-59 21.84755437956161 27.023271231887737 29.446065171717787 21.68310921683286 60-61 21.598173408249863 27.06809846419543 29.951256283185423 21.382471844369284 62-63 21.689951268079827 27.24817417503147 29.904423622063963 21.15745093482474 64-65 21.458088307288318 27.094758870778424 29.73337234088987 21.71378048104339 66-67 21.37757623873568 27.450427569219737 29.476795419128727 21.695200772915857 68-69 21.585374053762006 27.25065146944847 29.68447526775424 21.479499209035282 70-71 21.48893652110006 27.53306303298694 29.38230433208014 21.595696113832858 72-73 21.514147727138674 27.486734662580304 29.095149451809487 21.903968158471532 74-75 21.419336344622327 27.571343130049698 29.042089232144903 21.967231293183076 76-77 21.462571030519086 27.31641773789989 29.367735481580137 21.853275750000883 78-79 21.34366089850289 27.377701283120544 29.226470716610496 22.052167101766074 80-81 21.31322556709398 27.675330512363466 29.027461398444494 21.983982522098056 82-83 21.338331185357983 27.603714768534765 28.99117904677081 22.066774999336438 84-85 21.22336020611114 27.354553154543243 29.04106179323297 22.38102484611265 86-87 21.223158220075756 27.24953078864078 28.996613184632754 22.53069780665071 88-89 21.03376316357575 27.006284070171134 29.15067730409025 22.809275462162866 90-91 20.951936014572386 27.009174398514446 29.358606137933922 22.680283448979242 92-93 20.75646534562946 26.77232604983529 29.664943360947515 22.806265243587738 94-95 20.483272954197183 26.911675016721738 29.543269485985 23.061782543096076 96-97 20.68570329798667 26.832873460980842 29.381891449677305 23.099531791355187 98-99 20.58073747962128 26.93801330190705 29.151124575025538 23.330124643446133 100-101 20.30333454457936 26.703963605989532 29.490085086100713 23.50261676333039 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 314.0 1 244.5 2 263.5 3 391.5 4 314.0 5 247.0 6 539.0 7 1240.5 8 2083.5 9 2443.0 10 2104.5 11 1695.5 12 1690.0 13 1852.0 14 1988.0 15 2066.5 16 2136.0 17 2157.5 18 2254.5 19 2426.5 20 2505.5 21 2712.5 22 3103.5 23 3518.5 24 4038.0 25 4740.5 26 5559.5 27 6646.0 28 7896.5 29 9626.5 30 11735.0 31 13148.0 32 14540.0 33 16044.5 34 17804.5 35 19240.5 36 20881.5 37 22990.0 38 24137.0 39 24526.0 40 25155.5 41 26701.0 42 27890.0 43 29049.5 44 30779.0 45 31971.5 46 31482.5 47 31262.0 48 32841.5 49 32568.0 50 30706.0 51 29143.5 52 27306.5 53 26268.0 54 25020.5 55 22890.5 56 20437.5 57 18286.0 58 16920.0 59 15045.0 60 12608.5 61 10150.0 62 7943.5 63 6012.0 64 4209.0 65 3030.5 66 2222.0 67 1723.0 68 1268.5 69 790.0 70 560.5 71 411.0 72 314.0 73 273.5 74 226.5 75 176.0 76 142.5 77 90.5 78 49.5 79 29.0 80 22.5 81 13.5 82 7.5 83 6.5 84 3.5 85 0.5 86 0.5 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03869297946558861 2 0.0 3 0.0 4 0.0 5 8.257648056680495E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005721370439271487 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.898320040486069E-5 22-23 5.308488036437462E-4 24-25 5.898320040486069E-5 26-27 0.0018284792125506813 28-29 0.002831193619433313 30-31 1.1796640080972138E-4 32-33 1.1796640080972138E-4 34-35 0.0 36-37 0.0 38-39 5.898320040486069E-5 40-41 0.0 42-43 0.007549849651822168 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.1796640080972138E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.7694960121458206E-4 84-85 1.1796640080972138E-4 86-87 0.0 88-89 0.0 90-91 0.008080698455465914 92-93 7.66781605263189E-4 94-95 0.0 96-97 0.0 98-99 1.1796640080972138E-4 100-101 0.01026307687044576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 847699.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.17993752211833 #Duplication Level Percentage of deduplicated Percentage of total 1 79.62370290536198 46.32502060313672 2 11.624154223956843 13.52585132994554 3 3.6286406079737517 6.33342251586403 4 1.6324075689439173 3.7989348148716076 5 0.883562783376025 2.5702813766843056 6 0.5291434364151905 1.8471319242524864 7 0.37296118310406107 1.5189200831818728 8 0.26286017143450513 1.2234550680890257 9 0.19549416055072844 1.0236454242102315 >10 1.1242927008769015 12.002895973530826 >50 0.085661940080238 3.3732934052237438 >100 0.035282796716144434 3.8316434817507017 >500 8.157872043224075E-4 0.25190694583209383 >1k 6.118404032418056E-4 0.7825371209433154 >5k 4.0789360216120376E-4 1.5910599324835355 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7540 0.8894666621052991 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5869 0.6923448063522548 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3970 0.46832661121459385 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1456 0.1717590795789543 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1169 0.13790272254656427 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1796640080972138E-4 0.0 10-11 0.0 0.0 0.0 1.1796640080972138E-4 0.0 12-13 0.0 0.0 0.0 1.1796640080972138E-4 0.0 14-15 0.0 0.0 0.0 1.1796640080972138E-4 0.0 16-17 0.0 0.0 0.0 2.3593280161944276E-4 0.0 18-19 0.0 0.0 0.0 2.3593280161944276E-4 0.0 20-21 0.0 0.0 0.0 3.538992024291641E-4 0.0 22-23 0.0 0.0 0.0 4.718656032388855E-4 0.0 24-25 0.0 0.0 0.0 7.077984048583282E-4 0.0 26-27 0.0 0.0 0.0 9.43731206477771E-4 0.0 28-29 0.0 0.0 0.0 0.002241361615384706 0.0 30-31 0.0 0.0 0.0 0.005190521635627741 0.0 32-33 0.0 0.0 0.0 0.00955527846558743 0.0 34-35 0.0 0.0 0.0 0.012386472085020743 0.0 36-37 0.0 0.0 0.0 0.01728207771862418 0.0 38-39 0.0 0.0 0.0 0.025834641777328984 0.0 40-41 0.0 0.0 0.0 0.03568483624494072 0.0 42-43 0.0 0.0 0.0 0.04972283794129756 0.0 44-45 0.0 0.0 0.0 0.06228625962753288 0.0 46-47 0.0 0.0 0.0 0.08894666621052992 0.0 48-49 0.0 0.0 0.0 0.1264599816680213 0.0 50-51 0.0 0.0 0.0 0.1476349506133663 0.0 52-53 0.0 0.0 0.0 0.18497131646964313 0.0 54-55 0.0 0.0 0.0 0.25126843372470653 0.0 56-57 0.0 0.0 0.0 0.3491805463967753 0.0 58-59 0.0 0.0 0.0 0.4516933487004231 0.0 60-61 0.0 0.0 0.0 0.5942557440789714 0.0 62-63 0.0 0.0 0.0 0.7356384754494225 0.0 64-65 0.0 0.0 0.0 0.8702971219737194 0.0 66-67 0.0 0.0 0.0 1.0706040705486264 0.0 68-69 0.0 0.0 0.0 1.3393905147935765 0.0 70-71 0.0 0.0 0.0 1.499293971091154 0.0 72-73 0.0 0.0 0.0 1.7165880813826606 0.0 74-75 0.0 0.0 0.0 1.969567027919108 0.0 76-77 0.0 0.0 0.0 2.2276775128907786 0.0 78-79 0.0 0.0 0.0 2.5069039836073888 0.0 80-81 0.0 0.0 0.0 2.942082036194451 0.0 82-83 0.0 0.0 0.0 3.436420238787589 0.0 84-85 0.0 0.0 0.0 4.001951164269393 0.0 86-87 0.0 0.0 0.0 4.7221360412127416 0.0 88-89 0.0 0.0 0.0 5.549493393291723 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3130 0.0 49.190067 1 GTATCAA 5610 0.0 41.929443 1 CGGGACG 25 0.001616432 37.99809 62-63 TATAACG 80 1.0776455E-5 35.623207 2 TATCAAC 7230 0.0 32.51911 2 ATCAACG 7325 0.0 32.097363 3 TCAACGC 7420 0.0 31.622398 4 CAACGCA 7560 0.0 30.97397 5 AACGCAG 7725 0.0 30.250908 6 AGGACCG 160 6.240771E-8 26.717405 5 ACGCAGA 8840 0.0 26.168213 7 CGCAGAG 8805 0.0 26.056442 8 TACCTGG 570 0.0 25.832033 2 GGACCGA 185 8.965799E-9 25.674385 6 TAACGCA 115 1.281803E-4 24.781363 4 ATAACGC 115 1.281803E-4 24.781363 3 GTACATG 5305 0.0 24.633383 1 GCAGAGT 9255 0.0 24.378925 9 GAGTACT 5605 0.0 23.771393 12-13 TACATGG 5425 0.0 23.464258 2 >>END_MODULE