FastQCFastQC Report
Thu 26 May 2016
SRR937194_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937194_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816731
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51190.6267669526441386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36730.4497196751439581No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24650.30181295922402845No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11840.1449681718950303No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9260.11337882362736323No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG459.5882587E-442.2536352
GGTATCA32950.040.1204721
GTATCAA56000.037.2780461
TAGACCG600.00393474331.6921735
TATCAAC68350.030.322472
ATCAACG68000.030.2688273
TCAACGC68750.029.869484
CAACGCA69650.029.4853275
TGTACGC650.00582188629.252522
AACGCAG70850.029.1675136
GTACATG58450.027.0103511
TACATGG59700.026.116572
TACCTGG6400.025.9958882
GTGTACG1109.401934E-525.9379251
CGCGGAA559.492403E-525.90371382-83
ACGCAGA80100.025.5619967
CGCAGAG80700.025.2526598
ACATGGG59800.024.3241043
GAGTACT39700.024.28858812-13
GCAGAGT83450.023.9081699
Produced by FastQC (version 0.11.5)