##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937194_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 816731 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.862219017032537 33.0 31.0 34.0 30.0 34.0 2 31.95535372111503 33.0 31.0 34.0 30.0 34.0 3 31.987305489812435 34.0 31.0 34.0 30.0 34.0 4 35.61146938220785 37.0 35.0 37.0 33.0 37.0 5 35.62607639479829 37.0 35.0 37.0 33.0 37.0 6 35.68644143542978 37.0 35.0 37.0 35.0 37.0 7 35.62861578659314 37.0 35.0 37.0 33.0 37.0 8 35.621353909671605 37.0 35.0 37.0 33.0 37.0 9 37.2563806198124 39.0 37.0 39.0 34.0 39.0 10-11 37.14184841765527 39.0 37.0 39.0 34.0 39.0 12-13 37.01055610721278 39.0 37.0 39.0 33.0 39.0 14-15 38.198943715862384 40.0 38.0 41.0 33.0 41.0 16-17 38.1814165251472 40.0 38.0 41.0 33.0 41.0 18-19 38.17927138800903 40.0 38.0 41.0 33.5 41.0 20-21 38.14449004629431 40.0 38.0 41.0 34.0 41.0 22-23 38.00181516313205 40.0 38.0 41.0 33.0 41.0 24-25 37.84960225582229 40.0 38.0 41.0 33.0 41.0 26-27 37.70118068250134 40.0 37.0 41.0 33.0 41.0 28-29 37.7408179682172 40.0 37.0 41.0 33.0 41.0 30-31 37.5647477566053 40.0 37.0 41.0 33.0 41.0 32-33 37.352101242636806 40.0 36.5 41.0 32.0 41.0 34-35 37.23058326425714 39.5 36.5 41.0 32.0 41.0 36-37 37.25041109006515 40.0 37.0 41.0 32.0 41.0 38-39 37.163387333161104 40.0 36.5 41.0 32.0 41.0 40-41 36.96965279877952 40.0 36.0 41.0 31.0 41.0 42-43 36.7007509204377 39.5 36.0 41.0 31.0 41.0 44-45 36.43452189276518 39.0 36.0 41.0 30.0 41.0 46-47 36.02516495639323 39.0 35.0 41.0 29.5 41.0 48-49 35.66510087164562 39.0 35.0 41.0 27.5 41.0 50-51 34.75859860835453 38.0 34.0 40.0 25.5 40.5 52-53 34.821425904000215 38.5 34.5 40.0 26.0 40.5 54-55 35.131254354248824 39.0 35.0 40.0 26.0 41.0 56-57 34.93713842134069 38.5 35.0 40.0 26.0 41.0 58-59 34.70891884843357 38.0 34.5 40.0 25.5 41.0 60-61 34.422481820819826 38.0 34.0 40.0 24.5 41.0 62-63 34.102327449307055 37.5 34.0 40.0 23.5 41.0 64-65 33.73431092979206 37.0 34.0 40.0 22.0 41.0 66-67 33.364668415916626 36.5 33.0 39.5 21.5 41.0 68-69 32.9017736561977 36.0 33.0 39.0 20.0 41.0 70-71 32.46858512778381 35.5 32.0 39.0 18.5 40.0 72-73 32.083391594049935 35.0 32.0 38.0 17.5 40.0 74-75 31.63998244219945 35.0 31.5 37.0 13.5 39.5 76-77 31.209927748548786 35.0 31.0 37.0 9.0 39.0 78-79 30.767509743110033 35.0 31.0 36.0 7.0 39.0 80-81 30.301445029024244 34.5 30.5 36.0 3.5 37.5 82-83 29.83924266374118 34.0 30.0 35.0 2.0 37.0 84-85 29.433264440801196 34.0 29.5 35.0 2.0 36.5 86-87 29.026958692641763 34.0 29.0 35.0 2.0 36.0 88-89 28.65082873063469 34.0 29.0 35.0 2.0 36.0 90-91 28.25886062853008 34.0 29.0 35.0 2.0 35.5 92-93 27.83476750606993 34.0 27.5 35.0 2.0 35.0 94-95 27.34561379450517 33.5 26.5 35.0 2.0 35.0 96-97 26.769544684847276 33.0 25.5 35.0 2.0 35.0 98-99 26.06207062055928 33.0 24.5 35.0 2.0 35.0 100-101 24.194126952448237 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.004435149175424868 1101 2 -0.11602132290738609 1101 3 -0.03415806458449566 1101 4 0.011788183986361389 1101 5 0.07947965106235699 1101 6 -0.061040803142091704 1101 7 -0.07675514290282592 1101 8 0.08300607763484891 1101 9 -0.03445983802593844 1101 10-11 0.023371975366281106 1101 12-13 0.05552589698549326 1101 14-15 0.13929803202066182 1101 16-17 0.2797470459024325 1101 18-19 0.21396902287004593 1101 20-21 0.11568481645996087 1101 22-23 0.1947749295276111 1101 24-25 0.06764993138111208 1101 26-27 0.09506246882856573 1101 28-29 -0.06303887812620701 1101 30-31 0.10485185015056686 1101 32-33 0.19361654296179864 1101 34-35 0.14563025189549705 1101 36-37 -0.07705116386259903 1101 38-39 0.02441174170960636 1101 40-41 -0.03282657620662377 1101 42-43 0.19430261007869376 1101 44-45 0.1902771887039023 1101 46-47 0.15407791294556716 1101 48-49 0.2679218979537126 1101 50-51 0.15752005004033975 1101 52-53 0.2376614312774521 1101 54-55 0.15566981477838482 1101 56-57 0.15507537585576614 1101 58-59 0.167235065356806 1101 60-61 0.14140212649234485 1101 62-63 0.015446481041855975 1101 64-65 0.21755980441395195 1101 66-67 0.28715989275433884 1101 68-69 0.15575112245534228 1101 70-71 0.20291571584622403 1101 72-73 0.08717507860797369 1101 74-75 0.18320705956231365 1101 76-77 0.3452856510526843 1101 78-79 0.14499370983414295 1101 80-81 0.19922679787827846 1101 82-83 0.4500062296778857 1101 84-85 0.41516442824808664 1101 86-87 0.4762626819084481 1101 88-89 0.5420833213343634 1101 90-91 0.48156072476059464 1101 92-93 0.3214674751605635 1101 94-95 0.5807449059564753 1101 96-97 0.4123175143293736 1101 98-99 0.3956623692997212 1101 100-101 1.1379285553564458 1102 1 0.010023106300266704 1102 2 -0.07676112954883152 1102 3 -0.021886627005681447 1102 4 -0.0013475873461317178 1102 5 -0.04197631783213751 1102 6 -0.08094675101935422 1102 7 0.027020077778132645 1102 8 -0.020020404080156595 1102 9 -0.08278108644964277 1102 10-11 0.09988258906952296 1102 12-13 0.08189333078120598 1102 14-15 0.33904451163394356 1102 16-17 0.2750496940739353 1102 18-19 0.10591285431183195 1102 20-21 0.06522769161758646 1102 22-23 0.1967466088046237 1102 24-25 0.1193877939284036 1102 26-27 -0.04258596538749515 1102 28-29 0.043841638897752944 1102 30-31 0.11564682703161111 1102 32-33 0.005038096135400849 1102 34-35 0.13854218968448606 1102 36-37 0.0951706205005749 1102 38-39 0.39796023897736177 1102 40-41 0.4369125473747104 1102 42-43 0.34369967817453784 1102 44-45 0.2930462093007904 1102 46-47 0.5260159646479607 1102 48-49 0.5191198802992432 1102 50-51 0.31674924297855256 1102 52-53 0.2605150462417214 1102 54-55 0.17959268152912244 1102 56-57 -0.09136614369026574 1102 58-59 0.23125487409997447 1102 60-61 0.26752523494127445 1102 62-63 0.07078617208851057 1102 64-65 0.19346617263589394 1102 66-67 0.19283980027849879 1102 68-69 0.23396150496900248 1102 70-71 0.43092906178989665 1102 72-73 0.16009962673744127 1102 74-75 0.10631025418189921 1102 76-77 0.37575801338979886 1102 78-79 0.36474728276394686 1102 80-81 0.3207865393662992 1102 82-83 0.3448425579670804 1102 84-85 0.38786304111063075 1102 86-87 0.22951903962177056 1102 88-89 0.18967684802624873 1102 90-91 0.2829005734365104 1102 92-93 0.21791162186086055 1102 94-95 0.19849921448947683 1102 96-97 0.3956915685538398 1102 98-99 0.4858473209600831 1102 100-101 0.6485238685132373 1103 1 -0.04799021160836858 1103 2 -0.2173497133924016 1103 3 -0.1032655384359451 1103 4 0.0032374977248323944 1103 5 -0.16852748738153167 1103 6 -0.11926959744016585 1103 7 -0.11043795105852183 1103 8 -0.04760427586707294 1103 9 0.060638963774671595 1103 10-11 0.13289661237586614 1103 12-13 0.10846636765939621 1103 14-15 0.10796045644362806 1103 16-17 0.03067382996174217 1103 18-19 0.004531685608910152 1103 20-21 -0.26413942556308 1103 22-23 -0.3530530582476672 1103 24-25 -0.29679755637209837 1103 26-27 -0.11781169265252345 1103 28-29 -0.22062113021146956 1103 30-31 -0.273633244072478 1103 32-33 -0.14999209488629361 1103 34-35 -0.05392345744736815 1103 36-37 0.0020461319139073453 1103 38-39 -0.052834744234175446 1103 40-41 -0.1624667026464479 1103 42-43 -0.004684961085075656 1103 44-45 -0.010192271554714694 1103 46-47 0.15044318171293725 1103 48-49 0.28457091159068426 1103 50-51 0.48043395842110925 1103 52-53 0.3813115813282053 1103 54-55 0.4437810509317899 1103 56-57 0.5714558703612553 1103 58-59 0.5679898702908233 1103 60-61 0.5974150518200361 1103 62-63 0.5571000458505395 1103 64-65 0.4239802664820047 1103 66-67 0.4336110821272072 1103 68-69 0.3579527862533922 1103 70-71 0.49201579749149005 1103 72-73 0.45621993936970284 1103 74-75 0.5586676933060772 1103 76-77 0.7262532025774675 1103 78-79 0.4704038669461106 1103 80-81 0.5627640512875196 1103 82-83 0.3963803249601128 1103 84-85 0.29061127056239755 1103 86-87 0.4163492645025606 1103 88-89 0.37434643974205883 1103 90-91 0.47158952126529385 1103 92-93 0.41938731385601 1103 94-95 0.2815619102373397 1103 96-97 0.49667348215672646 1103 98-99 0.64130427759223 1103 100-101 0.670036382268492 1104 1 0.13852795853997435 1104 2 0.04507120775036455 1104 3 0.1701688584924277 1104 4 0.05247937685147264 1104 5 0.1563678896353764 1104 6 0.08447898504073947 1104 7 0.03789786713062426 1104 8 0.13735390372180944 1104 9 0.21919818872991925 1104 10-11 0.23383044805301978 1104 12-13 0.24182397390857346 1104 14-15 0.1918516830797472 1104 16-17 0.1338440358689823 1104 18-19 0.2048587943304767 1104 20-21 0.262214916794413 1104 22-23 0.3125808102411014 1104 24-25 0.25279834275234947 1104 26-27 0.2583323963647999 1104 28-29 0.20148871384704137 1104 30-31 0.3238805569510177 1104 32-33 0.04647677150137497 1104 34-35 0.2669373867895928 1104 36-37 0.32768336790997665 1104 38-39 0.3311459947753761 1104 40-41 0.24805762111778762 1104 42-43 0.30221439937636063 1104 44-45 0.2825988163516229 1104 46-47 0.22352816378168683 1104 48-49 0.24032992470957026 1104 50-51 0.16347740789986887 1104 52-53 0.20773528857957047 1104 54-55 0.3688108404194139 1104 56-57 0.47159920528719823 1104 58-59 0.3984049371516676 1104 60-61 0.3355388321779671 1104 62-63 0.1423800094699459 1104 64-65 0.1616442525744901 1104 66-67 0.44734662631062605 1104 68-69 0.3387918136482 1104 70-71 0.539070955534072 1104 72-73 0.6959404074819986 1104 74-75 0.5535387206548457 1104 76-77 0.4828622954228088 1104 78-79 0.5129215247728318 1104 80-81 0.6139947744669065 1104 82-83 0.3632413188640591 1104 84-85 0.40387680283002325 1104 86-87 0.544005853591834 1104 88-89 0.4934489846565491 1104 90-91 0.5816861428207964 1104 92-93 0.6590336680256748 1104 94-95 0.7367510375060888 1104 96-97 0.8735946882424166 1104 98-99 0.8562253570366245 1104 100-101 0.9124199154456321 1105 1 -0.0051670516402140265 1105 2 -0.05895011866982003 1105 3 0.07584316590348195 1105 4 0.08021503440590294 1105 5 0.16862996981733858 1105 6 0.12079748729994577 1105 7 0.2343341905242582 1105 8 0.07175590185084957 1105 9 0.08232665473176581 1105 10-11 0.19739959689162134 1105 12-13 0.11670092511093344 1105 14-15 0.08358402672369891 1105 16-17 -0.022144389901257 1105 18-19 0.11451117001297462 1105 20-21 0.105111409062971 1105 22-23 0.11843120736008217 1105 24-25 0.17200190367755397 1105 26-27 0.19230104872824683 1105 28-29 0.3815847683370137 1105 30-31 0.21425248531674157 1105 32-33 0.14941052997722437 1105 34-35 0.361982265208205 1105 36-37 0.23109159032104998 1105 38-39 0.30184356681624536 1105 40-41 2.6256385806533444E-4 1105 42-43 0.36907804602300587 1105 44-45 0.34825719620401685 1105 46-47 0.41797583517872994 1105 48-49 0.37033365438740873 1105 50-51 0.23149435042002864 1105 52-53 0.3024217205007176 1105 54-55 0.4395111638619653 1105 56-57 0.37205654938972543 1105 58-59 0.5681243858930571 1105 60-61 0.3976051042732536 1105 62-63 0.38252082009402955 1105 64-65 0.4824882603273295 1105 66-67 0.6010192889948982 1105 68-69 0.7133760462822778 1105 70-71 0.751562472045542 1105 72-73 0.512516685508082 1105 74-75 0.3189679698890373 1105 76-77 0.5148190617309751 1105 78-79 0.48073088932132535 1105 80-81 0.5453564737325927 1105 82-83 0.593609303554036 1105 84-85 0.7239465967194683 1105 86-87 0.7281823896382562 1105 88-89 0.55908749327455 1105 90-91 0.6591113505516226 1105 92-93 0.7459557972437239 1105 94-95 0.9444390652402141 1105 96-97 0.8969517957243944 1105 98-99 1.3372903634871314 1105 100-101 1.2771831726160912 1106 1 -0.011682043793385333 1106 2 -0.018396164109322655 1106 3 -0.04428630212046869 1106 4 -0.08450825634445636 1106 5 -0.2540700041190007 1106 6 -0.17366832770718332 1106 7 -0.11507327699320058 1106 8 -0.10572379714153612 1106 9 -0.1834035068444777 1106 10-11 -0.6278694041675621 1106 12-13 -0.6193411501483652 1106 14-15 -0.5244896096012823 1106 16-17 -0.454716670361087 1106 18-19 -0.30148988732155146 1106 20-21 -0.26036033404171377 1106 22-23 -0.3205178908555695 1106 24-25 -0.2899215763578624 1106 26-27 -0.40934863342442185 1106 28-29 -0.15679860045645455 1106 30-31 -0.17250273619668377 1106 32-33 -0.06913145747894589 1106 34-35 -0.1429074448196488 1106 36-37 -0.10205335138690685 1106 38-39 0.06055409930987565 1106 40-41 -0.1542815715399044 1106 42-43 -0.04981173424734919 1106 44-45 0.014972231611785958 1106 46-47 0.35915467074287477 1106 48-49 0.3273447804301952 1106 50-51 0.3955397085921035 1106 52-53 0.032052780996153274 1106 54-55 -0.024340750153818647 1106 56-57 0.05134175798223595 1106 58-59 0.19980689717345257 1106 60-61 0.16470776738835013 1106 62-63 0.12528815289375927 1106 64-65 0.3170070899393522 1106 66-67 0.2729766333107122 1106 68-69 0.143343808670366 1106 70-71 0.298725229470449 1106 72-73 0.3394031126231667 1106 74-75 -0.15605527483399584 1106 76-77 0.004922262755776785 1106 78-79 0.10358225433649082 1106 80-81 0.23112940929618375 1106 82-83 0.1680072051516852 1106 84-85 0.16127549614884273 1106 86-87 0.24559837128647644 1106 88-89 0.04735930588234183 1106 90-91 0.21032229433359007 1106 92-93 0.3606456303808123 1106 94-95 0.5551962424528121 1106 96-97 0.5766261108137805 1106 98-99 0.6878637641300323 1106 100-101 0.5023806372314326 1107 1 0.049449503430576414 1107 2 -0.013614527249057318 1107 3 -0.037436930401703705 1107 4 0.19383160069235572 1107 5 0.23542486893241943 1107 6 0.011738219982021292 1107 7 0.10406422944262061 1107 8 0.16535624280709982 1107 9 0.07870773315544 1107 10-11 0.0844512973735263 1107 12-13 0.2473690423370769 1107 14-15 0.44281433294877104 1107 16-17 0.4615938289762056 1107 18-19 0.1593859844871588 1107 20-21 0.018891911001411188 1107 22-23 0.21319704294589314 1107 24-25 0.3068572028928358 1107 26-27 0.2965160829547244 1107 28-29 0.2942296840886769 1107 30-31 0.5463091019989221 1107 32-33 0.48474492506027644 1107 34-35 0.34831036830260587 1107 36-37 0.2764346588525157 1107 38-39 0.35777702800817224 1107 40-41 0.32121473651950083 1107 42-43 0.4169287505537653 1107 44-45 0.41183413380379363 1107 46-47 0.32455681415682847 1107 48-49 0.5279502131967604 1107 50-51 0.5034920100843792 1107 52-53 0.4262380004138393 1107 54-55 0.44117201707506126 1107 56-57 0.3599454993416842 1107 58-59 0.3228779590173332 1107 60-61 0.44285377428218453 1107 62-63 0.3348262002490259 1107 64-65 0.5967431849393563 1107 66-67 0.6295482048512397 1107 68-69 0.7177398750115742 1107 70-71 0.6591893118820877 1107 72-73 0.509003378120326 1107 74-75 0.6675845174827231 1107 76-77 0.6898516806263828 1107 78-79 0.9899333433090582 1107 80-81 0.7870802468321862 1107 82-83 1.0155220980120028 1107 84-85 1.006638393955182 1107 86-87 0.8509210309724722 1107 88-89 0.8178030004282739 1107 90-91 0.7073256295899135 1107 92-93 0.6140361986150289 1107 94-95 0.8248753920348157 1107 96-97 0.7071032819870737 1107 98-99 0.9774226335753227 1107 100-101 0.9545037873841338 1108 1 0.17999740471649517 1108 2 0.06802908995658541 1108 3 0.07189636827078516 1108 4 0.16585163465998676 1108 5 0.01207689139264545 1108 6 0.06340237373752444 1108 7 0.1770339705261179 1108 8 0.16750738141581678 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-2.934168858346535 2316 96-97 -2.9857069469585262 2316 98-99 -3.0663981000897387 2316 100-101 -3.7771329371683997 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3001.0 3 533.0 4 479.0 5 586.0 6 567.0 7 670.0 8 920.0 9 1053.0 10 1288.0 11 1823.0 12 2473.0 13 3394.0 14 3924.0 15 5420.0 16 7176.0 17 9812.0 18 8283.0 19 4941.0 20 4621.0 21 4862.0 22 5223.0 23 5747.0 24 6243.0 25 7337.0 26 8279.0 27 9747.0 28 11613.0 29 14129.0 30 17373.0 31 21922.0 32 27932.0 33 37060.0 34 52379.0 35 75862.0 36 115871.0 37 169640.0 38 145273.0 39 19273.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56811202554969 19.32244196044712 14.570077386070507 21.53936862793269 2 15.039087679921812 20.66594552649576 40.76156822202932 23.533398571553107 3 19.172681700260412 26.144554793385282 29.605629064675064 25.077134441679238 4 12.179207763967149 16.652894236329992 37.58633349254508 33.58156450715777 5 13.060502808730085 38.74856493845351 33.27144918193818 14.91948307087823 6 30.768400167366277 36.823402811606186 17.750641427570528 14.657555593457008 7 28.4132162254674 33.94623163850819 20.144794004090354 17.495758131934057 8 27.255170687596852 34.53088057205467 19.33003446511588 18.8839142752326 9 27.38177468331135 16.751219212955863 20.15556850596571 35.71143759776707 10-11 24.81922861467609 27.750409400786957 26.50953693687834 20.92082504765861 12-13 26.14808775252409 25.0671628651166 27.630118656951286 21.154630725408026 14-15 22.881704255731606 25.931301699789707 26.604027767894166 24.58296627658452 16-17 21.472609798830973 29.856840679430114 26.860408619987336 21.81014090175158 18-19 21.43831641660665 27.526006936819 30.347172209537575 20.688504437036773 20-21 22.432702995149167 26.413905716718318 30.41492152056512 20.738469767567395 22-23 22.022826591027872 26.75002637706692 30.227382585247 20.999764446658208 24-25 21.51101133795193 26.957521781932265 30.481088590591675 21.050378289524126 26-27 21.367738928024878 27.266760920167695 30.244455962045073 21.121044189762355 28-29 21.195450134162396 27.26435083681264 30.431920712293742 21.108278316731223 30-31 21.187355246407044 27.152844456033254 30.463610288816973 21.196190008742732 32-33 20.905480871457396 27.38975651682064 30.234943569250287 21.469819042471677 34-35 21.497676273448167 27.238369863644035 30.137092197493747 21.126861665414054 36-37 21.51293884991817 27.098468733055096 30.05089910714943 21.3376933098773 38-39 21.366902723807012 27.28413653078813 30.123078904833907 21.225881840570953 40-41 21.47649360065067 27.273078569754933 30.045659691766414 21.204768137827987 42-43 21.323609695176334 27.379046455337637 29.739414839650752 21.557929009835277 44-45 21.351791201247973 27.634291785545372 29.523489368854527 21.490427644352128 46-47 21.53674667641662 27.87166131012157 28.97221201489731 21.6193799985645 48-49 21.5936112726657 28.083197750178527 28.582830878112585 21.740360099043187 50-51 21.72836188639147 28.288448465840627 28.383052710884026 21.600136936883878 52-53 21.966869691462037 28.323431630387695 28.159719111534372 21.5499795666159 54-55 21.482791178197395 28.31251644931586 28.372270793929104 21.832421578557636 56-57 21.646185693475427 28.60084336425669 28.164624702905773 21.58834623936211 58-59 21.790983257143946 28.061841290948603 28.29804577742312 21.84912967448433 60-61 21.58018824528068 28.22029334664683 28.610804783535098 21.58871362453739 62-63 21.68738180776537 28.18426671590129 28.68816510446921 21.44018637186413 64-65 21.337959535654242 28.20501182821881 28.688873991596047 21.7681546445309 66-67 21.369451499359386 28.4288573245759 28.329008385961536 21.87268279010318 68-69 21.381670540757685 28.32584069819502 28.526455237382624 21.766033523664667 70-71 21.333278538095776 28.421983031468823 28.40339763186433 21.84134079857107 72-73 21.417522010773226 28.38497293199324 28.199305910798866 21.998199146434665 74-75 21.289195122534693 28.62606446895704 28.175771787915277 21.90896862059299 76-77 21.43737808693092 28.426815354606017 28.23199980371229 21.903806754750775 78-79 21.30874232808971 28.394624786388746 28.167365720868208 22.12926716465333 80-81 21.293432669930723 28.674057617025767 28.043369183981664 21.989140529061842 82-83 21.32048067439658 28.59702301800321 28.08769265494585 21.994803652654358 84-85 21.220449381004805 28.319467145875564 28.057762662798652 22.402320810320983 86-87 21.48260091176779 28.17599215926313 28.09343871879023 22.24796821017885 88-89 21.29853434809248 28.09302108779744 28.14662529615712 22.46181926795296 90-91 21.034963713891766 28.090698096635375 28.425774177245007 22.448564012227852 92-93 21.2478096416859 28.045541053003454 28.438134222581496 22.268515082729152 94-95 20.851229557212207 28.11431094810376 28.365220964571456 22.669238530112576 96-97 20.965603273589025 28.175582519270115 28.160863144156657 22.697951062984206 98-99 20.963599728891737 28.325538459651177 27.834047394876542 22.87681441658054 100-101 20.87739623152977 28.14730679429805 28.24758099978596 22.727715974386225 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1336.0 1 776.0 2 254.0 3 307.0 4 301.0 5 306.5 6 466.5 7 878.5 8 1413.5 9 1697.0 10 1602.5 11 1469.0 12 1492.0 13 1573.0 14 1689.0 15 1770.0 16 1795.5 17 1888.5 18 2019.5 19 2144.5 20 2277.0 21 2505.5 22 2845.5 23 3292.5 24 3790.5 25 4437.0 26 5346.0 27 6597.5 28 7756.5 29 9193.5 30 11356.0 31 12937.0 32 14497.0 33 16025.0 34 17497.0 35 19068.5 36 20690.0 37 22459.0 38 23445.5 39 23723.5 40 24431.5 41 25929.0 42 26945.0 43 28228.0 44 29826.0 45 30698.5 46 30294.5 47 29886.0 48 31618.5 49 31423.0 50 29374.5 51 28006.5 52 25935.0 53 24689.5 54 24138.0 55 22410.5 56 19952.0 57 17829.5 58 16202.0 59 14340.0 60 11962.5 61 9634.0 62 7601.0 63 5636.0 64 4056.0 65 3062.5 66 2203.0 67 1659.0 68 1207.0 69 808.0 70 598.0 71 457.0 72 351.0 73 285.5 74 222.5 75 164.0 76 124.5 77 81.0 78 53.0 79 37.0 80 25.0 81 14.0 82 11.0 83 10.5 84 7.0 85 4.5 86 4.0 87 4.5 88 4.0 89 6.0 90 4.5 91 2.5 92 3.0 93 2.0 94 3.0 95 3.5 96 3.5 97 3.0 98 1.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3221378887295817 2 0.27916168236543 3 0.2747538663280811 4 0.28185534772158766 5 0.28234510505907084 6 0.21451371381764622 7 0.2010453870368579 8 0.20863662576784767 9 0.20275953771804914 10-11 0.2229008082220462 12-13 0.21237102546615713 14-15 0.20680003575228564 16-17 0.20753467175851048 18-19 0.20324929505553235 20-21 0.19749464634010463 22-23 0.19945367569003747 24-25 0.18959730927318785 26-27 0.19020950594504188 28-29 0.2049634457367236 30-31 0.21653396283476445 32-33 0.20882028476940387 34-35 0.21653396283476445 36-37 0.19455610231520537 38-39 0.196331472663582 40-41 0.1922297549621601 42-43 0.19639269233076742 44-45 0.2019636820446389 46-47 0.20624905874761704 48-49 0.21218736646460096 50-51 0.2151259104895002 52-53 0.23116546329207538 54-55 0.21151395012556154 56-57 0.18898511260133385 58-59 0.18947486993881707 60-61 0.1858629095748784 62-63 0.19425000397927836 64-65 0.1860465685764346 66-67 0.18359778188901854 68-69 0.18194485087501272 70-71 0.19284195163401413 72-73 0.1912502402871937 74-75 0.1952295186542448 76-77 0.196025374327655 78-79 0.19369902697460975 80-81 0.19467854164957618 82-83 0.18892389293414846 84-85 0.18347534255464773 86-87 0.18384266055776016 88-89 0.18323046388590614 90-91 0.18892389293414846 92-93 0.18494461456709735 94-95 0.18347534255464773 96-97 0.1810877755344171 98-99 0.1908217026168959 100-101 0.17955728385478203 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 816731.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.627145463778135 #Duplication Level Percentage of deduplicated Percentage of total 1 81.25678931169654 50.07623974831495 2 10.926349327395691 13.46719438774937 3 3.317096033411309 6.132694793050806 4 1.409916656470723 3.475565555204998 5 0.764298090408801 2.355075479765552 6 0.48827780733329673 1.8054700475558227 7 0.31789478958195283 1.3713663907820903 8 0.24090728292517594 1.1877142534490697 9 0.1803010698554428 1.0000296233330577 >10 0.9997274634898566 11.342447943857998 >50 0.06761784848565956 2.8441394467024717 >100 0.029432252934373508 3.1469876413838938 >500 5.965997190601574E-4 0.27035807766797965 >1k 5.965997190601574E-4 0.8973535107366034 >5k 1.9886657302005246E-4 0.6273631004453254 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5119 0.6267669526441386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3673 0.4497196751439581 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2465 0.30181295922402845 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1184 0.1449681718950303 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 926 0.11337882362736323 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.2243933437080262E-4 14-15 0.0 0.0 0.0 1.2243933437080262E-4 1.2243933437080262E-4 16-17 0.0 0.0 0.0 1.2243933437080262E-4 1.2243933437080262E-4 18-19 0.0 0.0 0.0 2.4487866874160525E-4 1.2243933437080262E-4 20-21 0.0 0.0 0.0 2.4487866874160525E-4 1.2243933437080262E-4 22-23 0.0 0.0 0.0 6.121966718540131E-4 1.2243933437080262E-4 24-25 0.0 0.0 0.0 6.121966718540131E-4 1.2243933437080262E-4 26-27 0.0 0.0 0.0 9.182950077810197E-4 1.2243933437080262E-4 28-29 0.0 0.0 0.0 0.0016529310140058354 1.2243933437080262E-4 30-31 0.0 0.0 0.0 0.0032446423608262697 1.2243933437080262E-4 32-33 0.0 0.0 0.0 0.007285140395062756 1.2243933437080262E-4 34-35 0.0 0.0 0.0 0.010652222090259829 1.2243933437080262E-4 36-37 0.0 0.0 0.0 0.015304916796350328 1.2243933437080262E-4 38-39 0.0 0.0 0.0 0.02369201120075031 1.2243933437080262E-4 40-41 0.0 0.0 0.0 0.035140088964420356 1.2243933437080262E-4 42-43 0.0 0.0 0.0 0.051485740102922506 1.2243933437080262E-4 44-45 0.0 0.0 0.0 0.06415821121030058 1.2243933437080262E-4 46-47 0.0 0.0 0.0 0.08925827475631512 1.2243933437080262E-4 48-49 0.0 0.0 0.0 0.12384738671606685 1.2243933437080262E-4 50-51 0.0 0.0 0.0 0.14196840820294565 1.2243933437080262E-4 52-53 0.0 0.0 0.0 0.17955728385478206 1.2243933437080262E-4 54-55 0.0 0.0 0.0 0.23520596132631183 1.2243933437080262E-4 56-57 0.0 0.0 0.0 0.31926056437186784 1.2243933437080262E-4 58-59 0.0 0.0 0.0 0.40276419041275524 1.2243933437080262E-4 60-61 0.0 0.0 0.0 0.5348762321988513 1.2243933437080262E-4 62-63 0.0 0.0 0.0 0.656274832227502 1.2243933437080262E-4 64-65 0.0 0.0 0.0 0.7782244092608215 1.2243933437080262E-4 66-67 0.0 0.0 0.0 0.9591897454608678 1.2243933437080262E-4 68-69 0.0 0.0 0.0 1.2055376862149227 1.2243933437080262E-4 70-71 0.0 0.0 0.0 1.3449348683960816 1.2243933437080262E-4 72-73 0.0 0.0 0.0 1.5393685313769159 1.2243933437080262E-4 74-75 0.0 0.0 0.0 1.7769620597234586 1.2243933437080262E-4 76-77 0.0 0.0 0.0 2.004638001985966 1.2243933437080262E-4 78-79 0.0 0.0 0.0 2.277065520961002 1.2243933437080262E-4 80-81 0.0 0.0 0.0 2.7130107709882445 1.2243933437080262E-4 82-83 0.0 0.0 0.0 3.1870958736719923 1.2243933437080262E-4 84-85 0.0 0.0 0.0 3.7454804580700376 1.2243933437080262E-4 86-87 0.0 0.0 0.0 4.494686745084979 1.2243933437080262E-4 88-89 0.0 0.0 0.0 5.323539819108127 1.2243933437080262E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 45 9.5882587E-4 42.253635 2 GGTATCA 3295 0.0 40.120472 1 GTATCAA 5600 0.0 37.278046 1 TAGACCG 60 0.003934743 31.692173 5 TATCAAC 6835 0.0 30.32247 2 ATCAACG 6800 0.0 30.268827 3 TCAACGC 6875 0.0 29.86948 4 CAACGCA 6965 0.0 29.485327 5 TGTACGC 65 0.005821886 29.25252 2 AACGCAG 7085 0.0 29.167513 6 GTACATG 5845 0.0 27.010351 1 TACATGG 5970 0.0 26.11657 2 TACCTGG 640 0.0 25.995888 2 GTGTACG 110 9.401934E-5 25.937925 1 CGCGGAA 55 9.492403E-5 25.903713 82-83 ACGCAGA 8010 0.0 25.561996 7 CGCAGAG 8070 0.0 25.252659 8 ACATGGG 5980 0.0 24.324104 3 GAGTACT 3970 0.0 24.288588 12-13 GCAGAGT 8345 0.0 23.908169 9 >>END_MODULE