FastQCFastQC Report
Thu 26 May 2016
SRR937194_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937194_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences816731
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75330.9223355058152561No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59300.7260652528188596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41170.5040827396045944No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15490.18965852894037327No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12060.14766183725118798No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34450.044.4204941
GTATCAA60600.040.0742151
ACGTAAC600.003940365731.6829411
TATCAAC77600.031.038692
ATCAACG78350.030.7415753
TCAACGC79100.030.4500924
CAACGCA80150.030.169735
AACGCAG81550.029.6463496
TAGGTCG700.00838418927.1468285
TTAGCCG908.9376804E-426.3927524
ACGCAGA92150.026.1346447
CGCAGAG92400.026.0639348
GTACATG52700.024.8894441
GCAGAGT96600.024.7340269
TACATGG52900.024.2474042
GAGTACT57350.023.7298612-13
AGTACTT58900.023.5489512-13
ACATGGG52500.022.9843163
GTACTTT59750.022.65605514-15
CCCGCAT1050.002193814622.6223583
Produced by FastQC (version 0.11.5)