##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937194_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 816731 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.274197991750036 33.0 31.0 34.0 31.0 34.0 2 32.483772502819164 34.0 31.0 34.0 31.0 34.0 3 32.552860121631234 34.0 31.0 34.0 31.0 34.0 4 36.162969202834226 37.0 35.0 37.0 35.0 37.0 5 36.034767873387935 37.0 35.0 37.0 35.0 37.0 6 36.128512325355594 37.0 35.0 37.0 35.0 37.0 7 36.09747150530591 37.0 35.0 37.0 35.0 37.0 8 36.07780652381261 37.0 35.0 37.0 35.0 37.0 9 37.794035000508124 39.0 38.0 39.0 35.0 39.0 10-11 37.72080403462094 39.0 37.0 39.0 35.0 39.0 12-13 37.631545147667964 39.0 37.0 39.0 35.0 39.0 14-15 38.85104826436121 40.0 38.0 41.0 35.0 41.0 16-17 38.837426276215794 40.0 38.0 41.0 35.0 41.0 18-19 38.8864632296313 40.0 38.0 41.0 35.0 41.0 20-21 38.8264985656232 40.0 38.0 41.0 35.0 41.0 22-23 38.70660964258734 40.0 38.0 41.0 34.5 41.0 24-25 38.56425922366115 40.0 38.0 41.0 34.0 41.0 26-27 38.34862457773735 40.0 38.0 41.0 34.0 41.0 28-29 38.309856611295515 40.0 38.0 41.0 34.0 41.0 30-31 38.3684775036089 40.0 38.0 41.0 34.0 41.0 32-33 38.266166583611984 40.0 37.5 41.0 34.0 41.0 34-35 38.133818846107225 40.0 37.0 41.0 34.0 41.0 36-37 37.96677853540517 40.0 37.0 41.0 33.5 41.0 38-39 37.81055329110809 40.0 37.0 41.0 33.0 41.0 40-41 37.62467446441974 40.0 37.0 41.0 33.0 41.0 42-43 37.3614280589325 40.0 37.0 41.0 33.0 41.0 44-45 36.98732508010593 40.0 36.0 41.0 31.5 41.0 46-47 36.504611065332405 40.0 36.0 41.0 30.5 41.0 48-49 36.07303077757548 39.5 36.0 41.0 29.5 41.0 50-51 35.813597132960545 39.0 35.0 41.0 28.5 41.0 52-53 35.57715637094711 39.0 35.0 41.0 28.0 41.0 54-55 35.37123055204222 39.0 35.0 40.5 27.5 41.0 56-57 35.1277617722359 39.0 35.0 40.0 27.0 41.0 58-59 34.869590477158326 38.0 35.0 40.0 26.0 41.0 60-61 34.54688753090063 38.0 34.0 40.0 26.0 41.0 62-63 34.192054666714014 37.5 34.0 40.0 25.5 41.0 64-65 33.841609416074576 37.0 34.0 40.0 24.5 41.0 66-67 33.451962763749634 36.5 33.5 39.0 22.5 41.0 68-69 33.027310705728084 36.0 33.0 39.0 22.0 40.0 70-71 32.647892635396474 35.5 33.0 38.5 20.5 40.0 72-73 32.2357630602977 35.0 32.0 38.0 19.5 40.0 74-75 31.80160236356891 35.0 32.0 37.0 18.0 39.0 76-77 30.487624444278474 34.0 30.5 36.0 17.0 38.5 78-79 31.09383995464847 35.0 31.0 36.0 16.0 39.0 80-81 30.919406144740435 35.0 31.0 36.0 12.5 37.5 82-83 30.58558570692186 34.5 31.0 35.5 7.5 37.0 84-85 30.276091516056084 34.0 31.0 35.0 6.5 37.0 86-87 29.962714773897403 34.0 31.0 35.0 2.0 36.0 88-89 29.618111103900794 34.0 30.5 35.0 2.0 36.0 90-91 29.209319225056966 34.0 30.0 35.0 2.0 35.5 92-93 28.84658841160676 34.0 29.5 35.0 2.0 35.0 94-95 28.481219642697532 34.0 29.0 35.0 2.0 35.0 96-97 28.103023516922953 34.0 29.0 35.0 2.0 35.0 98-99 27.51777451817061 34.0 28.0 35.0 2.0 35.0 100-101 26.006661924183113 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.027861709251155276 1101 2 0.08642912195548291 1101 3 0.1453281862150959 1101 4 0.07813313403478617 1101 5 -0.048189353219584063 1101 6 0.04195614225821487 1101 7 0.07636752903104593 1101 8 0.11467506274911443 1101 9 0.0811668511331618 1101 10-11 0.09744308470929752 1101 12-13 0.14405527759760872 1101 14-15 0.2394670046820977 1101 16-17 0.14422955425167316 1101 18-19 0.18004409222918838 1101 20-21 0.07425418354452518 1101 22-23 0.02493783079360412 1101 24-25 0.05066207631764286 1101 26-27 -0.08409631503126747 1101 28-29 -0.07912262647531776 1101 30-31 0.1932488951496225 1101 32-33 0.2196931775503046 1101 34-35 0.24259398824440837 1101 36-37 0.17608024707645598 1101 38-39 0.06933455940023947 1101 40-41 0.06566503215766062 1101 42-43 0.05537503359199292 1101 44-45 0.13325484380363406 1101 46-47 0.128178093276901 1101 48-49 0.0800638087709089 1101 50-51 0.04347317195178135 1101 52-53 0.2096662433088312 1101 54-55 0.03130900672017134 1101 56-57 0.09902583718198343 1101 58-59 0.055495412263049104 1101 60-61 -0.013131958428253654 1101 62-63 -0.13190086883211904 1101 64-65 -0.03784992291120659 1101 66-67 -0.176206051811711 1101 68-69 -0.17561493530553918 1101 70-71 -0.20602573286807768 1101 72-73 -0.25817322751224836 1101 74-75 -0.20328707499544052 1101 76-77 -0.18099109946689396 1101 78-79 -0.18461465364745067 1101 80-81 -0.2109760208942184 1101 82-83 -0.15374492640505366 1101 84-85 -0.11138700184809736 1101 86-87 -0.21869612693872043 1101 88-89 0.036026056706930376 1101 90-91 0.09225075295239904 1101 92-93 0.14972888619194435 1101 94-95 -0.07311276865163308 1101 96-97 -0.10456700997231394 1101 98-99 -0.09572028035584168 1101 100-101 0.0641908763197101 1102 1 0.10106999004140249 1102 2 0.10749101771000369 1102 3 0.15798581547108626 1102 4 0.030473928486237867 1102 5 0.04420503517890495 1102 6 0.06404525533093164 1102 7 0.09667017720254734 1102 8 0.04363418422872911 1102 9 0.07568398017897948 1102 10-11 0.06293278626456811 1102 12-13 0.060329184701423344 1102 14-15 0.09119173145806059 1102 16-17 0.023385712301283945 1102 18-19 0.10317749912284313 1102 20-21 0.1588143979203096 1102 22-23 0.1281587219243292 1102 24-25 0.02714641847821042 1102 26-27 -0.11906505189549677 1102 28-29 -0.14228204219621432 1102 30-31 -0.027592319646508656 1102 32-33 0.012816601257739535 1102 34-35 0.16563544473873293 1102 36-37 0.1635342718767916 1102 38-39 -0.0450661867115727 1102 40-41 -0.08845040500081325 1102 42-43 0.04091254935492117 1102 44-45 0.10534789760774999 1102 46-47 0.21352908108690372 1102 48-49 0.40014052167548897 1102 50-51 0.4326479827966736 1102 52-53 0.29883054553666 1102 54-55 0.24943374400474738 1102 56-57 0.3617596539116974 1102 58-59 0.2755064463109633 1102 60-61 0.3829391323661966 1102 62-63 0.4121065922860012 1102 64-65 0.5141007391316705 1102 66-67 0.46044800451448253 1102 68-69 0.31463306805722624 1102 70-71 0.3083172158498684 1102 72-73 0.28161502385387394 1102 74-75 0.3770657518225491 1102 76-77 0.3787884822901475 1102 78-79 0.37653367548243466 1102 80-81 0.4713852653605102 1102 82-83 0.2841386381178559 1102 84-85 0.2853750305184448 1102 86-87 0.1769808907157575 1102 88-89 0.3150968322428689 1102 90-91 0.2277948366009923 1102 92-93 0.30060898287121773 1102 94-95 0.3692658568212508 1102 96-97 0.3928504973837157 1102 98-99 0.315923267353277 1102 100-101 0.25618068296115837 1103 1 0.02740107550739168 1103 2 0.07880108232443206 1103 3 0.0615452522815616 1103 4 0.1288952484264243 1103 5 -0.022309735848999424 1103 6 7.583841929843516E-4 1103 7 0.0794457760851941 1103 8 0.05124965783274149 1103 9 -0.06633938259295036 1103 10-11 -0.030993739510662976 1103 12-13 0.0799865024971993 1103 14-15 0.03716673216344901 1103 16-17 -0.04331457832257257 1103 18-19 -0.014658481803209611 1103 20-21 -0.0907919901612928 1103 22-23 -0.050211668301678 1103 24-25 -0.09297152401776998 1103 26-27 -0.05228293513807358 1103 28-29 -0.03931090707023088 1103 30-31 0.10262127477802352 1103 32-33 0.24048582616673997 1103 34-35 0.3057704578591327 1103 36-37 0.2614526432545716 1103 38-39 0.34287997733571984 1103 40-41 0.369980508348128 1103 42-43 0.3946483355911994 1103 44-45 0.2794929555914436 1103 46-47 -0.06379308815211004 1103 48-49 -0.1066693877514453 1103 50-51 -0.18688024721940621 1103 52-53 -0.13834461882100868 1103 54-55 -0.25582993365672024 1103 56-57 -0.18988392273681143 1103 58-59 -0.02768759448924385 1103 60-61 -0.1328215685169667 1103 62-63 -0.14317260412494193 1103 64-65 0.060676738680214726 1103 66-67 -0.1340759931153812 1103 68-69 -0.1332366308790398 1103 70-71 -0.03901497003653276 1103 72-73 -0.019127759927627608 1103 74-75 0.09489397337386052 1103 76-77 -0.1189344939238417 1103 78-79 0.08673654786188223 1103 80-81 0.10145778024440233 1103 82-83 0.13644881559591582 1103 84-85 0.05615886844627127 1103 86-87 0.05959354272896533 1103 88-89 0.008398375653044354 1103 90-91 0.20275251273879746 1103 92-93 0.16172605067404788 1103 94-95 0.09265331767168306 1103 96-97 0.06432421426529444 1103 98-99 0.06928589820455144 1103 100-101 0.3538260654277927 1104 1 -0.08034620602197862 1104 2 0.015602756292160791 1104 3 -0.008308937530721039 1104 4 -0.004747300747823147 1104 5 -0.15636243014265716 1104 6 -0.14756448204501993 1104 7 -0.03985320675491266 1104 8 -0.07425330960317211 1104 9 -0.08664948108534531 1104 10-11 -0.052919234131280746 1104 12-13 -0.08317786064096566 1104 14-15 -0.08036646800463387 1104 16-17 -0.1171277478664905 1104 18-19 -0.1056826357529772 1104 20-21 -0.20762290675648387 1104 22-23 -0.19447409785745862 1104 24-25 -0.23964589469684938 1104 26-27 -0.17626117010373576 1104 28-29 -0.18820150049984363 1104 30-31 -0.23007189637768732 1104 32-33 -0.19789670539284288 1104 34-35 0.014803436404946524 1104 36-37 0.12478077661937448 1104 38-39 0.03817165527538435 1104 40-41 0.11466517151105649 1104 42-43 0.16103974931105824 1104 44-45 0.37093948705892643 1104 46-47 0.26335089149317525 1104 48-49 0.18074664043199817 1104 50-51 0.2681282722862335 1104 52-53 0.21888098689858282 1104 54-55 0.3759648038216241 1104 56-57 0.38348096538710763 1104 58-59 0.44188755273127356 1104 60-61 0.35418618538445656 1104 62-63 0.4732064332860588 1104 64-65 0.4446438061857805 1104 66-67 0.33243385900211564 1104 68-69 0.2234932029330352 1104 70-71 0.3774643730405103 1104 72-73 0.31523117605519246 1104 74-75 0.47419689936353393 1104 76-77 0.27488613018751096 1104 78-79 0.3857762326401719 1104 80-81 0.5325440459954116 1104 82-83 0.45104883056261613 1104 84-85 0.4818578420760957 1104 86-87 0.31229258543586624 1104 88-89 0.32318115704137895 1104 90-91 0.43556527357670305 1104 92-93 0.4680468906512516 1104 94-95 0.4435051243249539 1104 96-97 0.3162454203509846 1104 98-99 0.6255729191982269 1104 100-101 0.8285791148927366 1105 1 -0.14834709869897011 1105 2 -0.1733283547238429 1105 3 -0.10564741496617103 1105 4 -0.06610202979589985 1105 5 -0.17527678817950232 1105 6 -0.13106218615316578 1105 7 -0.02991147532839733 1105 8 -0.04454275706056876 1105 9 -0.09140176322019045 1105 10-11 -0.18323708216831136 1105 12-13 -0.17249141649136135 1105 14-15 -0.1304389215823747 1105 16-17 -0.13329528988651163 1105 18-19 -0.1680133481214412 1105 20-21 -0.12419276484529718 1105 22-23 -0.16028869618337893 1105 24-25 -0.2558187866645909 1105 26-27 -0.2620124250486171 1105 28-29 -0.3467640154032665 1105 30-31 -0.23302583179923175 1105 32-33 -0.3252262254872491 1105 34-35 -0.03564351351342765 1105 36-37 -0.10379497558326989 1105 38-39 -0.2029497243385734 1105 40-41 -0.20112160708671212 1105 42-43 -0.4336955964178486 1105 44-45 -0.1713663709809694 1105 46-47 0.052725742458918035 1105 48-49 0.03455626177798621 1105 50-51 -0.1273559132682962 1105 52-53 0.02620414819797645 1105 54-55 0.024560366116411103 1105 56-57 -0.07744225216316636 1105 58-59 -0.03291954343938386 1105 60-61 -0.1930798384732597 1105 62-63 -0.2477234793416585 1105 64-65 -0.18150719685298355 1105 66-67 -0.18653629134670524 1105 68-69 -0.08826200791605032 1105 70-71 -0.2896718152287434 1105 72-73 0.08572911861816124 1105 74-75 -0.06716507504231828 1105 76-77 -0.2738878146499424 1105 78-79 -0.18867746126944596 1105 80-81 -0.001977638106986035 1105 82-83 -0.2058941787371218 1105 84-85 -0.1314754504957314 1105 86-87 -0.07839193273256129 1105 88-89 -0.0235581262490534 1105 90-91 -0.13443955666181395 1105 92-93 -0.04414181881907098 1105 94-95 0.044447173691214203 1105 96-97 -0.34664061659116996 1105 98-99 -0.33989011113445144 1105 100-101 -0.2742445875156605 1106 1 -0.17774451044758166 1106 2 -0.05721749726621539 1106 3 -0.2029110997769692 1106 4 -0.009666799853363273 1106 5 -0.3018084906544445 1106 6 -0.07176919543072557 1106 7 -0.022858573481208566 1106 8 -0.004583091045191168 1106 9 -0.11739148932988286 1106 10-11 -0.1967416654916292 1106 12-13 -0.48445731930161884 1106 14-15 -0.2667629835214669 1106 16-17 -0.1048581670933757 1106 18-19 -0.20184620423707145 1106 20-21 -0.4343282034820035 1106 22-23 -0.16947798678955195 1106 24-25 -0.19466067263882536 1106 26-27 -0.32865869674291304 1106 28-29 -0.30359583821470437 1106 30-31 -0.13631946579513254 1106 32-33 -0.1969925286797647 1106 34-35 -0.22098251677230962 1106 36-37 -0.1275914719611677 1106 38-39 -0.09865069830632933 1106 40-41 -0.2301567153987989 1106 42-43 -0.3940275292142559 1106 44-45 -0.2703648607671596 1106 46-47 -0.2082112925310895 1106 48-49 -0.42037531647863347 1106 50-51 -0.4831064636552824 1106 52-53 -0.46983916554755467 1106 54-55 -0.3354375450933631 1106 56-57 -0.4748355159071309 1106 58-59 -0.49928262693739356 1106 60-61 -0.4624117768151237 1106 62-63 -0.4416842877219622 1106 64-65 -0.4674371613805235 1106 66-67 -0.22118479231622956 1106 68-69 -0.3895191638710358 1106 70-71 -0.44264335037863844 1106 72-73 -0.3904933319948931 1106 74-75 -0.466273272527264 1106 76-77 -0.36401680206729026 1106 78-79 -0.2809908981057241 1106 80-81 -0.4539516372899577 1106 82-83 -0.42786926852737395 1106 84-85 -0.3145371178679035 1106 86-87 -0.04153217928374531 1106 88-89 -0.16095199999369925 1106 90-91 -0.33053490531417395 1106 92-93 -0.090228756855943 1106 94-95 -0.21556192086443815 1106 96-97 -0.014974926541757583 1106 98-99 -0.10378657368502786 1106 100-101 -0.29869591038532306 1107 1 -0.08202699611300801 1107 2 0.03398201640254683 1107 3 0.0429115588751543 1107 4 0.03771017509188823 1107 5 -0.06795594909814184 1107 6 0.04203479571110336 1107 7 -0.09301268176020727 1107 8 -0.017477485622762856 1107 9 -0.05811366640655535 1107 10-11 -0.10792108077757234 1107 12-13 -0.079263996431024 1107 14-15 0.004261227585985239 1107 16-17 -0.04197266377568809 1107 18-19 0.01725356603758854 1107 20-21 -0.021344172598318778 1107 22-23 -0.18482109637329813 1107 24-25 -0.04516240850223596 1107 26-27 -0.05892475218715987 1107 28-29 -0.19790408785647173 1107 30-31 -0.3201566027267333 1107 32-33 -0.2619436991210833 1107 34-35 -0.1825500458911833 1107 36-37 -0.3215331122125207 1107 38-39 -0.38774277660730405 1107 40-41 -0.33576007009182973 1107 42-43 -0.33857553271285923 1107 44-45 -0.5321902364228919 1107 46-47 -0.505709746899285 1107 48-49 -0.4120020241691549 1107 50-51 -0.09539687288068421 1107 52-53 0.04039663893570378 1107 54-55 -0.2615522092622058 1107 56-57 -0.24458651927547237 1107 58-59 -0.1555170543092359 1107 60-61 -0.30294289519088125 1107 62-63 -0.27227270303063733 1107 64-65 -0.3210244745865296 1107 66-67 -0.18523153553044125 1107 68-69 -0.240189420125418 1107 70-71 -0.3219486328208916 1107 72-73 -0.03149889213392498 1107 74-75 -0.009698806157938833 1107 76-77 -0.06777191195705967 1107 78-79 0.09296567119131183 1107 80-81 0.05464652118537572 1107 82-83 -0.15637981707675408 1107 84-85 -0.30177505832127594 1107 86-87 -0.3421255158013885 1107 88-89 -0.16727099772125698 1107 90-91 -0.168915579437094 1107 92-93 -0.0955273274308368 1107 94-95 -0.17797256887186563 1107 96-97 -0.18996991228472737 1107 98-99 -0.028805855312583617 1107 100-101 0.06511997023193672 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-0.9036142093933037 2316 96-97 -0.391183250680573 2316 98-99 -0.6712756967093085 2316 100-101 -0.7397716852772156 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 3.0 5 19.0 6 26.0 7 69.0 8 169.0 9 389.0 10 681.0 11 1152.0 12 1870.0 13 2783.0 14 3626.0 15 5425.0 16 7764.0 17 11840.0 18 10335.0 19 5164.0 20 4017.0 21 3954.0 22 4086.0 23 4134.0 24 4716.0 25 5337.0 26 6469.0 27 7857.0 28 9687.0 29 12330.0 30 15429.0 31 19488.0 32 25425.0 33 33829.0 34 47824.0 35 71277.0 36 113162.0 37 186719.0 38 170857.0 39 18816.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.18551379373283 19.446829726011792 15.281086888083406 21.086569592171976 2 15.671867481459623 20.84860253865716 40.131205990711756 23.348323989171465 3 19.352638751314693 25.942201287816918 30.352466111853225 24.352693849015157 4 12.621429358539544 17.215358808908697 37.23482668691 32.92838514564176 5 13.029694095345857 38.454765762092116 33.37092699604593 15.144613146516095 6 30.86193618217014 37.08136461086943 17.213256261853658 14.843442945106775 7 28.19741138759273 34.25019988221336 19.936674376263422 17.615714353930485 8 27.74744683378003 34.07609114873808 19.081313186348993 19.095148831132892 9 27.437797757156275 16.36854729402949 20.21093848525402 35.982716463560216 10-11 24.953320003771132 27.58258227004975 26.308356117252806 21.155741608926316 12-13 26.41793374945281 24.910107784944394 27.773495495440336 20.898462970162456 14-15 23.158481801229534 25.511765807836362 27.182144426990035 24.147607963944065 16-17 21.310688586572567 29.257613583909514 27.606702818920798 21.824995010597124 18-19 21.23575571393764 26.430244474618934 31.795964644417808 20.53803516702562 20-21 22.083770543789814 25.324923383586516 32.260070941350335 20.33123513127333 22-23 21.59167643646671 25.578918046937893 32.10889934402042 20.720506172574975 24-25 21.39130605133404 25.818845663616695 32.20988846973721 20.579959815312055 26-27 21.218862952913632 26.088755889468995 32.00473361478709 20.687647542830277 28-29 20.91083682651319 26.264998429676123 32.18257877179245 20.641585972018238 30-31 20.999337595090612 25.927397483351104 32.28728047889059 20.785984442667694 32-33 20.786525796131162 26.036112257279324 32.122571568851924 21.05479037773759 34-35 21.299852705480752 25.798763607601522 32.083146103184525 20.818237583733197 36-37 21.311475409836063 25.718139064487733 31.942908847365526 21.027476678310673 38-39 21.238404952444387 25.97933461323721 31.987026280475263 20.79523415384314 40-41 21.179617279128625 26.078292607970067 31.89660977727061 20.845480335630704 42-43 21.190997504341073 26.120536162563155 31.56788118802182 21.12058514507395 44-45 21.347053068880697 26.391431205623395 31.159035227020894 21.102480498475018 46-47 21.397681733643022 26.90347250196209 30.520820196613087 21.178025567781802 48-49 21.67857083823856 26.972789677868036 30.000226512907258 21.348412970986143 50-51 21.79187517064982 27.100232512295968 29.77418513561993 21.333707181434278 52-53 21.93743460962289 27.36683320487383 29.298562131036043 21.39717005446724 54-55 21.51338690462343 27.247159713540935 29.514001550081975 21.725451831753663 56-57 21.65853873552002 27.654699037994153 29.209862243504897 21.47689998298093 58-59 21.735063319501773 27.03087062937491 29.553304576415 21.68076147470832 60-61 21.626520849582054 26.98201733496096 30.054142673658767 21.33731914179822 62-63 21.719942061706977 27.183368820333747 29.86601463639803 21.23067448156125 64-65 21.44831039840535 27.05395044390381 29.912664022793304 21.585075134897536 66-67 21.376499728796876 27.4523680379464 29.539530151298287 21.63160208195844 68-69 21.62119473853692 27.13390332924794 29.76653267722175 21.478369254993382 70-71 21.499061502502048 27.45457194596507 29.544856262343416 21.50151028918946 72-73 21.4828995103651 27.406453287557348 29.25167527619253 21.858971925885022 74-75 21.406925903388018 27.452674136282322 29.210719318845495 21.92968064148416 76-77 21.42314911519215 27.35496754745442 29.447210893182703 21.774672444170726 78-79 21.454371145456705 27.329071628235003 29.276836559405726 21.939720666902566 80-81 21.34644087220884 27.626905309091974 29.13076643350136 21.895887385197817 82-83 21.277498411345746 27.552468444245275 29.155631794634452 22.014401349774527 84-85 21.272513560172886 27.26249801035838 29.09407025577608 22.37091817369265 86-87 21.247754768705978 27.12784258219659 29.081117283414 22.543285365683438 88-89 21.043281080306738 27.01011716219906 29.22596301597466 22.720638741519544 90-91 20.869948540238717 27.00991541741795 29.468106274662265 22.652029767681064 92-93 20.760281176572864 26.755241450158014 29.692510569834376 22.79196680343475 94-95 20.563808646910672 26.860618734932306 29.6208298693205 22.95474274883652 96-97 20.622763186410214 26.87512779605525 29.3692170371885 23.132891980346038 98-99 20.596962842108805 26.97866765436029 29.088087374153243 23.33628212937766 100-101 20.15217436559319 27.05206403397381 29.268676473649602 23.527085126783398 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 295.0 1 225.0 2 255.0 3 380.5 4 318.5 5 274.5 6 560.5 7 1299.0 8 2124.0 9 2463.0 10 2173.5 11 1825.5 12 1822.5 13 1880.0 14 1939.0 15 2032.5 16 2084.0 17 2185.0 18 2335.5 19 2442.0 20 2563.0 21 2748.0 22 3055.5 23 3493.0 24 3920.0 25 4515.0 26 5338.0 27 6467.5 28 7640.0 29 9127.0 30 11196.5 31 12660.5 32 14115.0 33 15344.5 34 16737.0 35 18332.0 36 20152.5 37 22130.0 38 23043.5 39 23435.0 40 24010.0 41 25526.0 42 26875.0 43 28086.5 44 29653.5 45 30596.0 46 30276.5 47 30068.0 48 31655.5 49 31362.0 50 29413.0 51 28005.5 52 26194.0 53 25052.5 54 24029.0 55 22036.5 56 19557.5 57 17639.5 58 16381.0 59 14636.5 60 12298.5 61 9838.0 62 7654.5 63 5846.0 64 4109.5 65 2967.0 66 2208.5 67 1701.0 68 1224.5 69 758.5 70 567.5 71 410.5 72 298.0 73 258.5 74 216.0 75 184.0 76 134.5 77 76.5 78 53.5 79 36.0 80 18.0 81 13.5 82 13.0 83 5.0 84 2.0 85 1.0 86 0.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0467718257296466 2 0.0 3 0.0 4 1.2243933437080262E-4 5 0.012733690774563473 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0071627010606919546 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0016529310140058354 24-25 2.4487866874160525E-4 26-27 0.00232634735304525 28-29 0.0025100063546014536 30-31 0.0012243933437080263 32-33 0.0 34-35 0.0 36-37 2.4487866874160525E-4 38-39 3.673180031124079E-4 40-41 0.0 42-43 0.013835644783900697 44-45 0.0 46-47 0.0 48-49 6.121966718540131E-5 50-51 0.0 52-53 1.8365900155620395E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.4487866874160525E-4 84-85 1.2243933437080262E-4 86-87 0.0 88-89 0.0 90-91 0.008999291076253992 92-93 0.0024487866874160526 94-95 0.0 96-97 0.0 98-99 0.0016529310140058354 100-101 0.012488812105821869 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 816731.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.47376772879337 #Duplication Level Percentage of deduplicated Percentage of total 1 79.95732635510244 46.75406129503589 2 11.49130802977933 13.438801532666695 3 3.514837359110523 6.165773500233426 4 1.5884983980342988 3.7154194545687176 5 0.8654771479296417 2.530385486130821 6 0.5219606410011455 1.8312603171283848 7 0.35306564400274926 1.4451554922303524 8 0.2565879564947251 1.2002931656062632 9 0.20074289284669344 1.0564373960571245 >10 1.129198906785706 11.99354134758101 >50 0.08269946929734032 3.2449772516500484 >100 0.0366138062296194 3.922978134705917 >500 6.312725196862002E-4 0.1988373947551894 >1k 6.312725196862002E-4 0.8455510994787511 >5k 4.2084834645746675E-4 1.656527132171482 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7533 0.9223355058152561 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5930 0.7260652528188596 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4117 0.5040827396045944 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1549 0.18965852894037327 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1206 0.14766183725118798 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.2243933437080262E-4 0.0 20-21 0.0 0.0 0.0 1.2243933437080262E-4 0.0 22-23 0.0 0.0 0.0 3.673180031124079E-4 0.0 24-25 0.0 0.0 0.0 3.673180031124079E-4 0.0 26-27 0.0 0.0 0.0 6.734163390394144E-4 0.0 28-29 0.0 0.0 0.0 0.0014080523452642303 0.0 30-31 0.0 0.0 0.0 0.0030609833592700657 0.0 32-33 0.0 0.0 0.0 0.006856602724764947 0.0 34-35 0.0 0.0 0.0 0.01028490408714742 0.0 36-37 0.0 0.0 0.0 0.015060038127608723 0.0 38-39 0.0 0.0 0.0 0.0233246931976379 0.0 40-41 0.0 0.0 0.0 0.03483399062849335 0.0 42-43 0.0 0.0 0.0 0.051546959770107904 0.0 44-45 0.0 0.0 0.0 0.06446430954622759 0.0 46-47 0.0 0.0 0.0 0.08987047142816912 0.0 48-49 0.0 0.0 0.0 0.12445958338792087 0.0 50-51 0.0 0.0 0.0 0.14294792287791208 0.0 52-53 0.0 0.0 0.0 0.18072045753130467 0.0 54-55 0.0 0.0 0.0 0.23655279400439067 0.0 56-57 0.0 0.0 0.0 0.3221378887295817 0.0 58-59 0.0 0.0 0.0 0.40680468844699175 0.0 60-61 0.0 0.0 0.0 0.540936979250206 0.0 62-63 0.0 0.0 0.0 0.663131434952267 0.0 64-65 0.0 0.0 0.0 0.7849585726512156 0.0 66-67 0.0 0.0 0.0 0.9679441578683803 0.0 68-69 0.0 0.0 0.0 1.2166796656426657 0.0 70-71 0.0 0.0 0.0 1.3580970968409427 0.0 72-73 0.0 0.0 0.0 1.5549795465091933 0.0 74-75 0.0 0.0 0.0 1.7941647862025563 0.0 76-77 0.0 0.0 0.0 2.0239834168165527 0.0 78-79 0.0 0.0 0.0 2.2999004568211565 0.0 80-81 0.0 0.0 0.0 2.7404371818873043 0.0 82-83 0.0 0.0 0.0 3.219236198944328 0.0 84-85 0.0 0.0 0.0 3.78392640906247 0.0 86-87 0.0 0.0 0.0 4.539377102130322 1.2243933437080262E-4 88-89 0.0 0.0 0.0 5.3780253229031345 2.4487866874160525E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3445 0.0 44.420494 1 GTATCAA 6060 0.0 40.074215 1 ACGTAAC 60 0.0039403657 31.682941 1 TATCAAC 7760 0.0 31.03869 2 ATCAACG 7835 0.0 30.741575 3 TCAACGC 7910 0.0 30.450092 4 CAACGCA 8015 0.0 30.16973 5 AACGCAG 8155 0.0 29.646349 6 TAGGTCG 70 0.008384189 27.146828 5 TTAGCCG 90 8.9376804E-4 26.392752 4 ACGCAGA 9215 0.0 26.134644 7 CGCAGAG 9240 0.0 26.063934 8 GTACATG 5270 0.0 24.889444 1 GCAGAGT 9660 0.0 24.734026 9 TACATGG 5290 0.0 24.247404 2 GAGTACT 5735 0.0 23.72986 12-13 AGTACTT 5890 0.0 23.54895 12-13 ACATGGG 5250 0.0 22.984316 3 GTACTTT 5975 0.0 22.656055 14-15 CCCGCAT 105 0.0021938146 22.622358 3 >>END_MODULE