FastQCFastQC Report
Thu 26 May 2016
SRR937193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948365
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58120.6128442108260006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41020.43253388726914216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28220.2975647561856458No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13760.14509181591475856No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10920.1151455399556078No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35500.038.838691
GTATCAA61450.038.2208181
TATCAAC76600.030.2190112
TCAACGC77350.029.924424
ATCAACG77200.029.9209963
CAACGCA78950.029.2562225
AACGCAG79650.029.164236
ACGCAGA90000.025.7061397
CGCAGAG89850.025.5362138
GTACATG66600.025.1283171
TACATGG68600.024.1813962
GCAGAGT94500.023.927799
GAGTACT45550.023.62024112-13
AGTACTT47250.023.42386812-13
ACATGGG68600.023.0715713
GGACCGT1252.2543033E-422.8017756
CGCGGAA953.291825E-722.49818482-83
GTACTTT48100.022.26809114-15
GTATAGA2657.8398443E-1021.529321
GTAAGGT3351.8189894E-1221.2815654
Produced by FastQC (version 0.11.5)