##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937193_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 948365 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.802302910799114 33.0 31.0 34.0 30.0 34.0 2 31.879307017867593 33.0 31.0 34.0 30.0 34.0 3 31.9296926816152 33.0 31.0 34.0 30.0 34.0 4 35.56067442387688 37.0 35.0 37.0 33.0 37.0 5 35.549318036831814 37.0 35.0 37.0 33.0 37.0 6 35.62913013449463 37.0 35.0 37.0 33.0 37.0 7 35.57950050877036 37.0 35.0 37.0 33.0 37.0 8 35.56584226537251 37.0 35.0 37.0 33.0 37.0 9 37.19192294106172 39.0 37.0 39.0 34.0 39.0 10-11 37.1076958765876 39.0 37.0 39.0 33.5 39.0 12-13 36.999583493697045 39.0 37.0 39.0 33.0 39.0 14-15 38.17322338972864 40.0 38.0 41.0 33.0 41.0 16-17 38.125419010613 40.0 38.0 41.0 33.0 41.0 18-19 38.11129628360389 40.0 38.0 41.0 33.5 41.0 20-21 38.060517311372735 40.0 38.0 41.0 33.0 41.0 22-23 37.930090735107264 40.0 38.0 41.0 33.0 41.0 24-25 37.789528820654496 40.0 37.5 41.0 33.0 41.0 26-27 37.627024405160455 40.0 37.0 41.0 32.5 41.0 28-29 37.44380486416095 39.5 37.0 41.0 32.0 41.0 30-31 37.40912992360536 39.0 37.0 41.0 32.0 41.0 32-33 37.242185761811115 39.0 36.0 41.0 32.0 41.0 34-35 37.04623747185946 39.0 36.0 41.0 31.0 41.0 36-37 37.054640881938916 39.0 36.0 41.0 31.0 41.0 38-39 37.036319876840665 40.0 36.0 41.0 31.0 41.0 40-41 36.86356466128548 39.5 36.0 41.0 31.0 41.0 42-43 36.60752821962008 39.0 36.0 41.0 30.0 41.0 44-45 36.34049232099456 39.0 35.5 41.0 30.0 41.0 46-47 35.959792906739494 39.0 35.0 41.0 29.0 41.0 48-49 35.61080754772688 39.0 35.0 41.0 27.5 41.0 50-51 34.655263005277504 38.0 34.0 40.0 25.5 40.5 52-53 34.758621944082705 38.0 34.5 40.0 26.0 40.5 54-55 35.1040691084129 39.0 35.0 40.0 26.0 41.0 56-57 34.90000685390119 38.5 35.0 40.0 25.5 41.0 58-59 34.660237355870365 38.0 34.0 40.0 25.0 41.0 60-61 34.39067711271504 38.0 34.0 40.0 24.5 41.0 62-63 34.0693894228488 37.5 34.0 40.0 23.0 41.0 64-65 33.71193527808386 37.0 34.0 40.0 22.0 41.0 66-67 33.33112989197197 36.5 33.0 39.5 21.5 41.0 68-69 32.863878886293776 36.0 33.0 39.0 20.0 41.0 70-71 32.44857886994986 35.5 32.0 39.0 18.5 40.0 72-73 32.04413806920331 35.0 32.0 38.0 17.5 40.0 74-75 31.596986919593192 35.0 31.5 37.0 14.0 39.5 76-77 31.171820448877806 35.0 31.0 37.0 9.5 39.0 78-79 30.725887712009616 35.0 31.0 36.0 7.0 39.0 80-81 30.291338777791253 34.5 30.5 36.0 4.0 37.5 82-83 29.87015916867451 34.0 30.0 35.0 2.0 37.0 84-85 29.440281431727236 34.0 29.5 35.0 2.0 36.5 86-87 29.040077396361106 34.0 29.0 35.0 2.0 36.0 88-89 28.686596405392436 34.0 29.0 35.0 2.0 36.0 90-91 28.283174727030207 34.0 29.0 35.0 2.0 35.5 92-93 27.868759918385855 34.0 27.5 35.0 2.0 35.0 94-95 27.37204399150116 33.5 26.5 35.0 2.0 35.0 96-97 26.801141965382527 33.0 25.5 35.0 2.0 35.0 98-99 26.13716659724895 33.0 24.5 35.0 2.0 35.0 100-101 24.35934318537694 31.0 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18526306826782246 1101 2 0.20569667529352031 1101 3 0.21224081953794283 1101 4 0.11794456033529599 1101 5 0.15536090498476085 1101 6 0.1596364025414374 1101 7 0.2279777628885924 1101 8 0.2309335185841519 1101 9 0.1797547921073317 1101 10-11 0.23846718021051316 1101 12-13 0.364793440749132 1101 14-15 0.33169758483909817 1101 16-17 0.3234289524153766 1101 18-19 0.3530234557268912 1101 20-21 0.22078994652138562 1101 22-23 0.32108443636102635 1101 24-25 0.322943583971842 1101 26-27 0.34258108084580385 1101 28-29 0.34782157457058105 1101 30-31 0.2694735134528585 1101 32-33 0.3516673116503455 1101 34-35 0.14735093840349123 1101 36-37 0.1757429499081411 1101 38-39 0.26343559560391583 1101 40-41 0.3618293714967393 1101 42-43 0.443686417786715 1101 44-45 0.3747045716855908 1101 46-47 0.35070967446187495 1101 48-49 0.2679334925511654 1101 50-51 0.40978848285759284 1101 52-53 0.5079535508125517 1101 54-55 0.6998090498311171 1101 56-57 0.7773016926846523 1101 58-59 0.5221813567953077 1101 60-61 0.5410175278488154 1101 62-63 0.6104229754937691 1101 64-65 0.5074782167537037 1101 66-67 0.7670273845723585 1101 68-69 0.5668306914193977 1101 70-71 0.5927930907939256 1101 72-73 0.7083449330172833 1101 74-75 0.8801988473124247 1101 76-77 0.7068020026129282 1101 78-79 0.7830531008571526 1101 80-81 0.7585917663203894 1101 82-83 0.5157159968933662 1101 84-85 0.483829284571776 1101 86-87 0.5743850688398275 1101 88-89 0.7745473292416882 1101 90-91 0.743877128243831 1101 92-93 0.8493461795555213 1101 94-95 0.9102479163378163 1101 96-97 0.7402357284735714 1101 98-99 0.8418637550642849 1101 100-101 1.029231681584843 1102 1 -0.024295176259450102 1102 2 -0.06341740768546344 1102 3 0.06993457424072602 1102 4 -0.1299659817753991 1102 5 0.0011577097164163774 1102 6 -0.0812806622213671 1102 7 -0.04345544882855279 1102 8 -0.03395899545857617 1102 9 0.14902209835263136 1102 10-11 -0.2050974972878734 1102 12-13 0.047922312343452234 1102 14-15 0.0812467190893571 1102 16-17 0.11066597929342947 1102 18-19 0.04804736336858895 1102 20-21 0.028326322009164073 1102 22-23 0.02317919855327233 1102 24-25 0.10195319952472204 1102 26-27 0.11376192804688401 1102 28-29 -0.19974271139368938 1102 30-31 -0.09254816966261359 1102 32-33 0.0580951971911432 1102 34-35 0.012439274186647253 1102 36-37 0.16891407000193936 1102 38-39 0.1931965082502458 1102 40-41 0.22261575354653473 1102 42-43 0.21841103150222096 1102 44-45 0.1313043275782917 1102 46-47 0.14446279142339336 1102 48-49 0.29542454632476733 1102 50-51 0.3991984693835704 1102 52-53 0.3059785455629367 1102 54-55 0.46743574064603166 1102 56-57 0.40213846233135797 1102 58-59 0.42068269982293316 1102 60-61 0.5001631851011652 1102 62-63 0.6613880305272843 1102 64-65 0.6374650817684895 1102 66-67 0.6364187677595723 1102 68-69 0.7497438364663793 1102 70-71 0.7432534714788162 1102 72-73 0.5912691241384316 1102 74-75 0.35111021405083775 1102 76-77 0.39043040830351217 1102 78-79 0.41837379791911644 1102 80-81 0.42282173464768746 1102 82-83 0.5839725326695238 1102 84-85 0.3902736845402792 1102 86-87 0.3044969404166409 1102 88-89 0.6482040281935149 1102 90-91 0.5523745668670301 1102 92-93 0.40637290449295804 1102 94-95 0.3687950622947369 1102 96-97 0.43901393424115653 1102 98-99 0.7076490325066693 1102 100-101 0.672647729233887 1103 1 0.11838923774122634 1103 2 0.05861367609544743 1103 3 0.04892664329273089 1103 4 -0.03943036169980729 1103 5 0.164516211052657 1103 6 0.12793030791433324 1103 7 0.13011901299997675 1103 8 0.16260397211707556 1103 9 0.35477873846748764 1103 10-11 0.18840898498641678 1103 12-13 0.34084067633676796 1103 14-15 0.2991012981955947 1103 16-17 0.23995945896442805 1103 18-19 0.3595329674439043 1103 20-21 0.3312868056499596 1103 22-23 0.2415777870693958 1103 24-25 0.19114393147251718 1103 26-27 0.17922987127617063 1103 28-29 0.012244522980097372 1103 30-31 0.01958349964193218 1103 32-33 0.0867668282680043 1103 34-35 0.16124790222777818 1103 36-37 0.39985860525876404 1103 38-39 0.325387335334824 1103 40-41 0.39558566437742115 1103 42-43 0.4163975912697424 1103 44-45 0.3686394598616616 1103 46-47 0.35028170288564553 1103 48-49 0.2100306368084901 1103 50-51 0.13036787339488143 1103 52-53 0.3278254099411271 1103 54-55 0.2769018503300913 1103 56-57 0.2942654612848443 1103 58-59 0.21620089257005048 1103 60-61 0.3396484086293583 1103 62-63 0.4561148582345709 1103 64-65 0.30780316329205704 1103 66-67 0.46634101506331405 1103 68-69 0.5896398779114236 1103 70-71 0.5291066321821418 1103 72-73 0.7153612165421883 1103 74-75 0.642972559777899 1103 76-77 0.6544418939075918 1103 78-79 0.6929532501042353 1103 80-81 0.5032901535608794 1103 82-83 0.4834769565298238 1103 84-85 0.4038453787533669 1103 86-87 0.6322007709693835 1103 88-89 0.565569950198654 1103 90-91 0.5003295585209422 1103 92-93 0.6002711773279508 1103 94-95 0.3875863062513858 1103 96-97 0.5085900566389476 1103 98-99 0.47028362942942437 1103 100-101 1.000481291760373 1104 1 0.08621087354711676 1104 2 0.19878442673298835 1104 3 0.08804467805884642 1104 4 0.01629150941179347 1104 5 0.24688861006215035 1104 6 0.22588462657493835 1104 7 0.09747927713371496 1104 8 0.18977315815642726 1104 9 0.14709803969575574 1104 10-11 0.12105826859755808 1104 12-13 0.15739677395783502 1104 14-15 0.2772046232747485 1104 16-17 0.32852434051079626 1104 18-19 0.2591285742492957 1104 20-21 0.24643630485533663 1104 22-23 0.27456462966096495 1104 24-25 0.11159889691584368 1104 26-27 0.25504112159956094 1104 28-29 0.2930119768737285 1104 30-31 0.34110688853698434 1104 32-33 0.12400826917670571 1104 34-35 0.20453121769758553 1104 36-37 0.15102897398651294 1104 38-39 0.1451246799529784 1104 40-41 0.05404619831195845 1104 42-43 0.19954243363955726 1104 44-45 0.18628153646537982 1104 46-47 0.06894908409321943 1104 48-49 0.15998043414990804 1104 50-51 -0.09474023171654977 1104 52-53 -0.014581360949954103 1104 54-55 0.09198451532128615 1104 56-57 0.26063214396709355 1104 58-59 -0.07168548310599476 1104 60-61 0.13611235511270792 1104 62-63 0.22149565925956693 1104 64-65 0.28074454629830115 1104 66-67 0.39665541306259655 1104 68-69 0.48078511451070227 1104 70-71 0.43029332447371615 1104 72-73 0.4272958976475074 1104 74-75 0.3771294631054438 1104 76-77 0.4782678292785967 1104 78-79 0.6103695687402002 1104 80-81 0.7090609953639877 1104 82-83 0.634834276262243 1104 84-85 0.6423985566124522 1104 86-87 0.4236370597917478 1104 88-89 0.5929325550698472 1104 90-91 0.6291064616020918 1104 92-93 0.4280658946531055 1104 94-95 0.49714175092990587 1104 96-97 0.6704157086575258 1104 98-99 0.7219089020021414 1104 100-101 0.7236404249483321 1105 1 0.06439265960671747 1105 2 0.07053968112489883 1105 3 -0.06660533430821047 1105 4 -0.028364798910104128 1105 5 0.004320085339287516 1105 6 0.014567808344935429 1105 7 0.045489466301461334 1105 8 -0.014944150258635602 1105 9 0.1466941027966442 1105 10-11 0.0538316848918754 1105 12-13 0.06676915398938377 1105 14-15 0.10266172244567429 1105 16-17 0.26945639880619865 1105 18-19 0.31212903038479567 1105 20-21 0.05693127612201465 1105 22-23 0.25308811582110735 1105 24-25 0.19391127906744998 1105 26-27 0.22993319094501175 1105 28-29 0.09259061367101395 1105 30-31 0.22084009745025668 1105 32-33 0.12755094836247594 1105 34-35 0.1767768177566964 1105 36-37 0.03261282421987488 1105 38-39 0.17400576671187906 1105 40-41 0.4085148807549217 1105 42-43 0.23531775156921242 1105 44-45 0.19394710241068225 1105 46-47 -0.21822756562362144 1105 48-49 -0.1910893160356011 1105 50-51 -0.18664270223638368 1105 52-53 -0.2987778718345666 1105 54-55 0.07665458801386649 1105 56-57 0.20159070309285454 1105 58-59 0.18531836513579236 1105 60-61 0.3345477621494055 1105 62-63 0.36347225179886067 1105 64-65 0.3190964676888086 1105 66-67 0.35736110608340965 1105 68-69 0.5713015112971931 1105 70-71 0.48120663945456243 1105 72-73 0.5946149130372582 1105 74-75 0.3246682587149792 1105 76-77 0.4187136374896774 1105 78-79 0.3513986361358299 1105 80-81 0.44012534901676403 1105 82-83 0.3338840207299931 1105 84-85 0.3170647264554738 1105 86-87 0.11545047146046272 1105 88-89 0.29094916868131904 1105 90-91 0.20749672086051874 1105 92-93 0.1897652082012904 1105 94-95 0.21459110222170352 1105 96-97 0.30570405375294385 1105 98-99 0.2571875301264299 1105 100-101 0.27212650478489664 1106 1 -0.14028998169564488 1106 2 -0.03995545753925356 1106 3 -0.19228247406985943 1106 4 -0.11188691060123546 1106 5 -0.10284035984932416 1106 6 -0.1514211322918939 1106 7 -0.06760870313473788 1106 8 -0.10361604174742212 1106 9 -0.1607449370367604 1106 10-11 -0.37070152837373627 1106 12-13 -0.36233695117305587 1106 14-15 -0.48629157777522636 1106 16-17 -0.632085721045776 1106 18-19 -0.43780413504132554 1106 20-21 -0.5333587155764263 1106 22-23 -0.3129022318593897 1106 24-25 -0.39863701770619997 1106 26-27 -0.04501017554765241 1106 28-29 -0.11093825182967976 1106 30-31 -0.06597168231926531 1106 32-33 -0.0914131860946199 1106 34-35 -0.14979098064208785 1106 36-37 -0.4078129471918075 1106 38-39 -0.3850056444122103 1106 40-41 -0.03056012874262848 1106 42-43 -0.10945015657897983 1106 44-45 -0.07773895800777808 1106 46-47 -0.26818465079198006 1106 48-49 -0.2885974417127386 1106 50-51 -0.12393780150331679 1106 52-53 -0.10485911453552887 1106 54-55 -0.01999524103032968 1106 56-57 -0.17155024047062994 1106 58-59 -0.2086327541674322 1106 60-61 -0.04324346071224028 1106 62-63 0.10537119792315508 1106 64-65 0.026424351073032426 1106 66-67 -0.06813593208968882 1106 68-69 -0.037132308404224545 1106 70-71 0.1837365039562613 1106 72-73 0.18880945089377477 1106 74-75 0.15326923520327895 1106 76-77 0.11014896272781982 1106 78-79 0.11462836415428868 1106 80-81 0.15912465956536792 1106 82-83 0.04110637583967147 1106 84-85 0.04821385901305675 1106 86-87 0.25169450911295144 1106 88-89 0.31712721598612603 1106 90-91 0.19976501594571516 1106 92-93 -0.027081325546649992 1106 94-95 0.07325356651796966 1106 96-97 0.22763423624650514 1106 98-99 0.3384583719606553 1106 100-101 1.1275527686618645 1107 1 0.06860482762070319 1107 2 -0.06471535706239351 1107 3 -0.09779456974415979 1107 4 0.039677371255422145 1107 5 0.11112430235584014 1107 6 0.06376936512991449 1107 7 -0.23628538170189728 1107 8 -0.008386501638291577 1107 9 -0.04771613812522446 1107 10-11 -0.08583451341942094 1107 12-13 0.12855418392405937 1107 14-15 0.009038965021098022 1107 16-17 -0.07127213454113956 1107 18-19 0.02766324339120274 1107 20-21 0.10144591214322674 1107 22-23 0.13631762645203338 1107 24-25 0.23744055060480918 1107 26-27 0.18505137912689662 1107 28-29 0.22538097800839552 1107 30-31 0.318844721744064 1107 32-33 0.006511219638213106 1107 34-35 0.043631904025332346 1107 36-37 0.07892798529742606 1107 38-39 0.0028978049062473588 1107 40-41 0.14698167180007005 1107 42-43 0.0810094713761984 1107 44-45 0.16372757471896193 1107 46-47 0.05067491713123218 1107 48-49 -0.16561681078554358 1107 50-51 -0.20731212381581088 1107 52-53 -0.006565534121719452 1107 54-55 0.012182659537884888 1107 56-57 0.09917037316999 1107 58-59 -0.18146500316425573 1107 60-61 -0.06759913039183374 1107 62-63 0.14025108469498093 1107 64-65 0.10860625012073655 1107 66-67 0.15000577183221964 1107 68-69 0.08964413934659632 1107 70-71 0.22880484509118304 1107 72-73 0.07516116153093222 1107 74-75 0.0502727856339682 1107 76-77 0.3510765223298158 1107 78-79 0.3590926610189342 1107 80-81 0.5338698249654854 1107 82-83 0.6159119018478627 1107 84-85 0.4409039812350706 1107 86-87 0.4797661355739038 1107 88-89 0.4520150997169168 1107 90-91 0.4975898431073311 1107 92-93 0.27436513809950114 1107 94-95 0.30069912968463086 1107 96-97 0.5840647224068398 1107 98-99 0.4543195184616522 1107 100-101 0.43729854204995533 1108 1 -0.052742425556790096 1108 2 -0.052025143038502364 1108 3 -0.005121525044650355 1108 4 -0.021756842035721036 1108 5 0.16146400713684983 1108 6 0.11954542397974421 1108 7 0.11893644560989713 1108 8 -0.01011061789401424 1108 9 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-1.0191253916630956 2316 96-97 -1.061520586963983 2316 98-99 -1.0876583673141376 2316 100-101 -1.5868868521246746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3523.0 3 604.0 4 501.0 5 590.0 6 685.0 7 848.0 8 946.0 9 1227.0 10 1567.0 11 2068.0 12 2960.0 13 3955.0 14 4676.0 15 6180.0 16 8208.0 17 11410.0 18 9496.0 19 5704.0 20 5587.0 21 5649.0 22 6084.0 23 6672.0 24 7470.0 25 8384.0 26 9713.0 27 11837.0 28 14005.0 29 17028.0 30 20756.0 31 26397.0 32 33523.0 33 44698.0 34 61583.0 35 89328.0 36 134420.0 37 193405.0 38 164029.0 39 22646.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.55072239984178 19.306123873514192 14.601244382774087 21.54190934386994 2 15.103382504694398 20.58895674386345 40.690288092297806 23.61737265914435 3 19.283615482296568 26.03393823279695 29.53740680942759 25.145039475478892 4 12.214731606638358 16.511203498502816 37.6508813926342 33.62318350222463 5 13.10811453702578 38.74087504427024 33.240370231368175 14.910640187335803 6 30.86600162509364 36.75596228683854 17.686893693727963 14.691142394339856 7 28.462331799708775 34.01370751800089 20.064416458320565 17.45954422396977 8 27.334581627671557 34.48240517858992 19.326935472622413 18.85607772111611 9 27.451579158697786 16.715096313359187 20.04997939538668 35.78334513255634 10-11 24.821806546106266 27.75221044422347 26.463722507484388 20.962260502185874 12-13 26.09022322001757 24.965272952742154 27.665766502150195 21.278737325090074 14-15 22.905691397079178 25.84547877005133 26.61440800637332 24.634421826496176 16-17 21.531044357384577 29.8354991163394 26.853085151067656 21.780371375208365 18-19 21.578252538790416 27.46954587308874 30.19368396807276 20.758517620048085 20-21 22.594972285441987 26.377697758110973 30.303063922048445 20.724266034398596 22-23 22.086848932812714 26.675429360554038 30.137468282341185 21.100253424292067 24-25 21.67315284038413 26.834270029440027 30.378166731026557 21.114410399149286 26-27 21.503566422741418 27.110413024562114 30.13520231742193 21.250818235274537 28-29 21.31257360007312 27.299267604979 30.323467863744835 21.06469093120305 30-31 21.371243200976366 27.03268022200678 30.37768097488555 21.218395602131306 32-33 21.01463517726 27.217995456226557 30.145770592275582 21.62159877423786 34-35 21.629587444344185 27.12966034897014 30.031264462058953 21.209487744626717 36-37 21.61592319034364 27.01217769470655 29.923550639842738 21.44834847510707 38-39 21.450018911820205 27.206660764206582 29.99652402857692 21.34679629539629 40-41 21.598889390147143 27.147078679819593 29.98820927895937 21.265822651073897 42-43 21.46924290679081 27.30212678739997 29.619506794087613 21.609123511721602 44-45 21.47958070428198 27.52620069917841 29.395396115687237 21.59882248085237 46-47 21.65983262316334 27.690132035622256 28.905943768905434 21.74409157230897 48-49 21.727080423237936 27.907436249099238 28.54658301088948 21.818900316773348 50-51 21.85647851203603 28.138924962514544 28.332824369404257 21.671772156045172 52-53 22.052476963395044 28.171390227407215 28.09594006255812 21.680192746639616 54-55 21.56737592399833 28.226134556348708 28.26703089417148 21.939458625481485 56-57 21.762508128163777 28.457790861487638 28.070914036117305 21.708786974231277 58-59 21.84117067935403 27.989656308891007 28.20969213997786 21.959480871777103 60-61 21.714352420664305 28.03685693428652 28.63041542227326 21.618375222775914 62-63 21.82887006789713 28.128925319492616 28.61948131112142 21.422723301488837 64-65 21.534124611839175 28.07825596878973 28.560608021347722 21.827011398023373 66-67 21.406169191864496 28.351594596265056 28.32909047975501 21.913145732115435 68-69 21.457355365527324 28.194313344450496 28.48863466494539 21.85969662507679 70-71 21.389405980133837 28.407801515846238 28.318369968749142 21.884422535270787 72-73 21.56925001558483 28.35372392974463 28.05523476041093 22.021791294259604 74-75 21.360231077321945 28.567029010388552 28.09419316804769 21.978546744241818 76-77 21.532049863149457 28.344200902193993 28.17367260786258 21.95007662679397 78-79 21.498401965186613 28.38231332047862 27.985366755593123 22.133917958741648 80-81 21.449651241716825 28.652533218533183 27.8690221826643 22.028793357085696 82-83 21.43506556283239 28.64261877303654 27.903283662199662 22.01903200193141 84-85 21.448344965173145 28.2759611519615 27.888790702415932 22.386903180449423 86-87 21.58134105243141 28.148208795968603 28.001943860464625 22.268506291135363 88-89 21.440577866618206 27.981037827147137 28.06291705581311 22.515467250421544 90-91 21.16344289252754 28.051034535947146 28.31848356453614 22.46703900698917 92-93 21.29677907750109 27.983689097591242 28.36737248985323 22.35215933505444 94-95 20.98904018844093 28.006129929788955 28.290228192500084 22.714601689270033 96-97 21.064046809227676 28.07476080630448 28.063087023560442 22.798105360907407 98-99 21.061497932143457 28.187560320186282 27.857506221530326 22.893435526139935 100-101 20.962446348530932 27.980264499233588 28.30061513660723 22.75667401562825 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1515.0 1 888.5 2 295.0 3 349.5 4 331.0 5 346.0 6 562.0 7 1013.5 8 1608.0 9 1923.5 10 1809.5 11 1678.5 12 1710.5 13 1802.0 14 1883.0 15 1978.0 16 2054.5 17 2113.5 18 2253.5 19 2388.0 20 2580.5 21 2848.0 22 3147.5 23 3602.5 24 4182.5 25 4983.0 26 6070.5 27 7510.5 28 8910.5 29 10745.0 30 13028.5 31 14493.5 32 16341.0 33 18255.0 34 20010.5 35 21709.5 36 23782.5 37 25982.5 38 26961.5 39 27283.5 40 28153.0 41 30113.0 42 31335.5 43 32774.0 44 34471.0 45 35354.5 46 35051.5 47 34708.5 48 36762.5 49 36908.5 50 34707.5 51 32738.5 52 30330.0 53 28897.5 54 28123.0 55 26295.5 56 23738.0 57 21378.5 58 19116.5 59 16784.5 60 14201.5 61 11521.5 62 9165.0 63 6830.0 64 4859.5 65 3642.5 66 2713.5 67 2080.5 68 1504.5 69 996.5 70 733.0 71 561.5 72 416.5 73 337.0 74 262.0 75 195.0 76 141.0 77 89.0 78 65.5 79 43.5 80 26.5 81 18.5 82 9.5 83 6.5 84 4.0 85 3.5 86 3.5 87 3.5 88 6.5 89 4.5 90 1.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2990409810568716 2 0.2696219282660157 3 0.27289071190944414 4 0.27299615654310316 5 0.26044824513768433 6 0.2064605927042858 7 0.2133144938921196 8 0.2116273797535759 9 0.20825315147648846 10-11 0.21742683460481987 12-13 0.21684688911969546 14-15 0.20234825199158551 16-17 0.21273454840699518 18-19 0.20039752626889437 20-21 0.20361358759549328 22-23 0.20334997601134586 24-25 0.196601519457171 26-27 0.20756776135770508 28-29 0.20556431331818448 30-31 0.21062565573381556 32-33 0.21247093682284773 34-35 0.20277003052622142 36-37 0.20261186357573296 38-39 0.19686513104131848 40-41 0.19591612933838762 42-43 0.1943871821503324 44-45 0.20756776135770508 46-47 0.1980250220115673 48-49 0.205300701734037 50-51 0.21067837805064504 52-53 0.21563427583261718 54-55 0.21827039167409173 56-57 0.19090750923958602 58-59 0.17936132185392756 60-61 0.18832411571494098 62-63 0.18832411571494098 64-65 0.18489716512102408 66-67 0.19733963189278392 68-69 0.1877441702298166 70-71 0.19306912422959516 72-73 0.20371903222915227 74-75 0.19422901519984395 76-77 0.19976485846694048 78-79 0.19929035761547506 80-81 0.18900950583372436 82-83 0.20103019407084824 84-85 0.18489716512102408 86-87 0.1892731174178718 88-89 0.19496712763545682 90-91 0.19291095727910668 92-93 0.19839407822937372 94-95 0.1962851855561941 96-97 0.18974761826933723 98-99 0.19544162848692226 100-101 0.1841063303685817 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 948365.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.8992431249335 #Duplication Level Percentage of deduplicated Percentage of total 1 80.26338657490267 48.07716106480619 2 11.433456422643948 13.697107720365647 3 3.40140878966498 6.1122543617828535 4 1.5694481167828653 3.7603501727658353 5 0.8457854049448827 2.5330952801156936 6 0.52216140845091 1.876624389315526 7 0.34671493084815086 1.4537573356542528 8 0.2565683148864222 1.2294598297229038 9 0.18187557313591335 0.9804788256379229 >10 1.0658010344726812 11.72867380140366 >50 0.07800702675393448 3.1472750842163713 >100 0.03415404030595276 3.651438631858807 >500 3.521034876325138E-4 0.14900602448256312 >1k 7.042069752650276E-4 0.9904207940129035 >5k 1.760517438162569E-4 0.612896683858922 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5812 0.6128442108260006 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4102 0.43253388726914216 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2822 0.2975647561856458 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1376 0.14509181591475856 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1092 0.1151455399556078 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.0544463365898152E-4 0.0 14-15 0.0 0.0 0.0 1.0544463365898152E-4 0.0 16-17 0.0 0.0 0.0 1.0544463365898152E-4 0.0 18-19 0.0 0.0 0.0 1.0544463365898152E-4 0.0 20-21 0.0 0.0 0.0 2.636115841474538E-4 0.0 22-23 0.0 0.0 0.0 3.1633390097694455E-4 0.0 24-25 0.0 0.0 0.0 5.799454851243983E-4 0.0 26-27 0.0 0.0 0.0 0.0013707802375667597 0.0 28-29 0.0 0.0 0.0 0.002319781940497593 0.0 30-31 0.0 0.0 0.0 0.004006896079041297 0.0 32-33 0.0 0.0 0.0 0.007592013623446669 0.0 34-35 0.0 0.0 0.0 0.010808074950045606 0.0 36-37 0.0 0.0 0.0 0.016502085167630607 0.0 38-39 0.0 0.0 0.0 0.02525398976132607 0.0 40-41 0.0 0.0 0.0 0.03484945142429339 0.0 42-43 0.0 0.0 0.0 0.048662698433619964 0.0 44-45 0.0 0.0 0.0 0.05962894033415404 0.0 46-47 0.0 0.0 0.0 0.08567376484792248 0.0 48-49 0.0 0.0 0.0 0.11472376142097188 0.0 50-51 0.0 0.0 0.0 0.13048773415298962 0.0 52-53 0.0 0.0 0.0 0.16212112425068406 0.0 54-55 0.0 0.0 0.0 0.2173741122879904 0.0 56-57 0.0 0.0 0.0 0.2987773694727241 0.0 58-59 0.0 0.0 0.0 0.38534741370674797 0.0 60-61 0.0 0.0 0.0 0.5131463097014335 0.0 62-63 0.0 0.0 0.0 0.6314551886668107 0.0 64-65 0.0 0.0 0.0 0.7518729603053677 0.0 66-67 0.0 0.0 0.0 0.9251712157239038 0.0 68-69 0.0 0.0 0.0 1.1604181934170916 0.0 70-71 0.0 0.0 0.0 1.3042973960447717 0.0 72-73 0.0 0.0 0.0 1.4986845781951041 0.0 74-75 0.0 0.0 0.0 1.7152151334138228 0.0 76-77 0.0 0.0 0.0 1.937966922018421 0.0 78-79 0.0 0.0 0.0 2.187817981473378 0.0 80-81 0.0 0.0 0.0 2.581231909655038 0.0 82-83 0.0 0.0 0.0 3.0273681546661884 0.0 84-85 0.0 0.0 0.0 3.5697753502079896 0.0 86-87 0.0 0.0 0.0 4.26033225604066 0.0 88-89 0.0 0.0 0.0 5.048109114106911 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3550 0.0 38.83869 1 GTATCAA 6145 0.0 38.220818 1 TATCAAC 7660 0.0 30.219011 2 TCAACGC 7735 0.0 29.92442 4 ATCAACG 7720 0.0 29.920996 3 CAACGCA 7895 0.0 29.256222 5 AACGCAG 7965 0.0 29.16423 6 ACGCAGA 9000 0.0 25.706139 7 CGCAGAG 8985 0.0 25.536213 8 GTACATG 6660 0.0 25.128317 1 TACATGG 6860 0.0 24.181396 2 GCAGAGT 9450 0.0 23.92779 9 GAGTACT 4555 0.0 23.620241 12-13 AGTACTT 4725 0.0 23.423868 12-13 ACATGGG 6860 0.0 23.071571 3 GGACCGT 125 2.2543033E-4 22.801775 6 CGCGGAA 95 3.291825E-7 22.498184 82-83 GTACTTT 4810 0.0 22.268091 14-15 GTATAGA 265 7.8398443E-10 21.52932 1 GTAAGGT 335 1.8189894E-12 21.281565 4 >>END_MODULE