FastQCFastQC Report
Thu 26 May 2016
SRR937193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948365
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86540.9125178596848259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66590.7021558155351578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45980.48483442556399703No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16260.17145297432950396No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.14667348541964328No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39100.045.211591
GTATCAA67050.038.6970141
CGTAGAC600.00394826731.67053
TATCAAC85350.030.5016382
ATCAACG85850.030.1026543
TCAACGC87200.029.6366144
CAACGCA88550.029.292085
CGGTATA650.00584010929.2343062
AACGCAG90600.028.7805256
GTACATG60450.027.6713281
GTGTACG1056.88405E-527.1547341
TACATGG62000.026.2814062
ACATGGG61250.025.2846933
ACGCAGA103250.025.1623867
CGCAGAG103650.025.0194578
GTACGCT950.001225531725.0030253
GAGTACT63500.023.6356712-13
GCAGAGT109300.023.5088639
TACCTGG6750.023.2250332
ATGGGAG16750.022.972935
Produced by FastQC (version 0.11.5)