##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937193_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 948365 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22184812809414 33.0 31.0 34.0 31.0 34.0 2 32.424381962640965 34.0 31.0 34.0 31.0 34.0 3 32.49955449642279 34.0 31.0 34.0 31.0 34.0 4 36.110785404353805 37.0 35.0 37.0 35.0 37.0 5 36.01333031058717 37.0 35.0 37.0 35.0 37.0 6 36.103274583098276 37.0 35.0 37.0 35.0 37.0 7 36.059456011134955 37.0 35.0 37.0 35.0 37.0 8 36.03984120038171 37.0 35.0 37.0 35.0 37.0 9 37.742715093872086 39.0 37.0 39.0 35.0 39.0 10-11 37.67352179804189 39.0 37.0 39.0 35.0 39.0 12-13 37.59385574119669 39.0 37.0 39.0 35.0 39.0 14-15 38.79438032824915 40.0 38.0 41.0 35.0 41.0 16-17 38.779015990678694 40.0 38.0 41.0 35.0 41.0 18-19 38.82242069245491 40.0 38.0 41.0 35.0 41.0 20-21 38.742565362492286 40.0 38.0 41.0 35.0 41.0 22-23 38.622561461040846 40.0 38.0 41.0 34.0 41.0 24-25 38.48827192062128 40.0 38.0 41.0 34.0 41.0 26-27 38.41782646976638 40.0 38.0 41.0 34.0 41.0 28-29 38.31087608673876 40.0 38.0 41.0 34.0 41.0 30-31 38.32774090144618 40.0 38.0 41.0 34.0 41.0 32-33 38.20936981014694 40.0 37.0 41.0 34.0 41.0 34-35 38.074290489421266 40.0 37.0 41.0 34.0 41.0 36-37 37.898443637207194 40.0 37.0 41.0 33.0 41.0 38-39 37.7588449594829 40.0 37.0 41.0 33.0 41.0 40-41 37.56424846973475 40.0 37.0 41.0 33.0 41.0 42-43 37.300211416490484 40.0 36.5 41.0 33.0 41.0 44-45 36.91856669109467 40.0 36.0 41.0 31.5 41.0 46-47 36.43544205026546 40.0 36.0 41.0 30.5 41.0 48-49 36.02805459923131 39.0 35.5 41.0 29.5 41.0 50-51 35.7733188171221 39.0 35.0 41.0 28.5 41.0 52-53 35.52779995044102 39.0 35.0 41.0 28.0 41.0 54-55 35.29136092116432 39.0 35.0 40.0 27.0 41.0 56-57 35.040095321948826 38.5 35.0 40.0 26.5 41.0 58-59 34.78764452505101 38.0 34.5 40.0 26.0 41.0 60-61 34.468079273275585 38.0 34.0 40.0 26.0 41.0 62-63 34.10743279222662 37.5 34.0 40.0 25.0 41.0 64-65 33.74962698960843 37.0 34.0 40.0 23.5 41.0 66-67 33.336348346891754 36.0 33.0 39.0 22.5 41.0 68-69 32.930532548122294 36.0 33.0 39.0 21.5 40.0 70-71 32.52217184311947 35.0 32.5 38.5 20.5 40.0 72-73 32.08830566290405 35.0 32.0 38.0 19.0 40.0 74-75 31.655311509808985 35.0 31.5 37.0 18.0 39.0 76-77 30.3177115351157 34.0 30.0 35.5 15.0 38.5 78-79 30.958737933179734 34.5 31.0 36.0 15.0 38.5 80-81 30.814482820433064 35.0 31.0 36.0 12.0 37.5 82-83 30.497972299694737 34.0 31.0 35.5 7.5 37.0 84-85 30.143525435881756 34.0 31.0 35.0 4.5 36.5 86-87 29.780781133846148 34.0 30.5 35.0 2.0 36.0 88-89 29.418112751946772 34.0 30.0 35.0 2.0 36.0 90-91 28.9949597465111 34.0 29.5 35.0 2.0 35.5 92-93 28.64803530286335 34.0 29.0 35.0 2.0 35.0 94-95 28.255517126844623 34.0 29.0 35.0 2.0 35.0 96-97 27.80697199917753 34.0 29.0 35.0 2.0 35.0 98-99 27.339716248490824 33.5 27.5 35.0 2.0 35.0 100-101 25.505862721631438 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21180880266609137 1101 2 0.2466211786578114 1101 3 0.21323510106226706 1101 4 0.15790180228220407 1101 5 0.09062156526292853 1101 6 0.12979992496006076 1101 7 0.07017606619573513 1101 8 0.13221079189374763 1101 9 0.1821943413658289 1101 10-11 0.15114643470175082 1101 12-13 0.1541354618280053 1101 14-15 0.15675086530088578 1101 16-17 0.2394966984009912 1101 18-19 0.10652645063024124 1101 20-21 0.22039258837719444 1101 22-23 0.1701501270156598 1101 24-25 0.15602309362779465 1101 26-27 0.13504797405665414 1101 28-29 0.1561103347182069 1101 30-31 0.2844019598054146 1101 32-33 0.16273691948624247 1101 34-35 0.03973773807815206 1101 36-37 0.0627272614796226 1101 38-39 0.18367726982211252 1101 40-41 0.2057112933234606 1101 42-43 0.48798260808002425 1101 44-45 0.4550613658086249 1101 46-47 0.3068328102165978 1101 48-49 0.3792695234168093 1101 50-51 0.44008781564726007 1101 52-53 0.34946977504306886 1101 54-55 0.3797489972402346 1101 56-57 0.4389563239734784 1101 58-59 0.11269085485521657 1101 60-61 0.3123164210795366 1101 62-63 0.4017404304569965 1101 64-65 0.2603759427474799 1101 66-67 0.34271046459809895 1101 68-69 0.27043574596932984 1101 70-71 0.2545448365908385 1101 72-73 0.2583196701240382 1101 74-75 0.25083300653082574 1101 76-77 0.12998438737781726 1101 78-79 0.17224724820118098 1101 80-81 0.3885447531764612 1101 82-83 0.28358042185570653 1101 84-85 0.3455977024560397 1101 86-87 0.2988183723918034 1101 88-89 0.27194787806954324 1101 90-91 0.31063585779952874 1101 92-93 0.24177285139116478 1101 94-95 0.32134015867134735 1101 96-97 0.2729837622732276 1101 98-99 0.3456112573150243 1101 100-101 0.7002278295606779 1102 1 0.12332920181214746 1102 2 0.2078275849433595 1102 3 0.19936079444949684 1102 4 0.12094666902936524 1102 5 0.04145286436874329 1102 6 0.016186677545498185 1102 7 0.07911846474684125 1102 8 0.13366592077190376 1102 9 0.07932476815988565 1102 10-11 0.0909762267293246 1102 12-13 0.13012747584448903 1102 14-15 0.2156663595525501 1102 16-17 0.07546140345986885 1102 18-19 0.13562109908072983 1102 20-21 0.09041146513362719 1102 22-23 0.05832311728637052 1102 24-25 0.0727195628212769 1102 26-27 0.08435360252358493 1102 28-29 -0.012236210157290373 1102 30-31 0.018037091570860753 1102 32-33 0.1481339547636793 1102 34-35 0.11644588767480712 1102 36-37 0.19448860721956152 1102 38-39 0.25344607877096337 1102 40-41 0.3525983783847906 1102 42-43 0.28681801282568387 1102 44-45 0.4053797333519711 1102 46-47 0.3551801644084236 1102 48-49 0.2058163565839024 1102 50-51 0.05258934678625593 1102 52-53 0.20340513035481678 1102 54-55 0.16411923977264564 1102 56-57 0.09085023354003852 1102 58-59 0.1622770645425149 1102 60-61 -0.04812818887373993 1102 62-63 0.012868858020993912 1102 64-65 -0.04003188705986105 1102 66-67 -0.07747946225720881 1102 68-69 -0.007417039391228286 1102 70-71 0.08125353302284566 1102 72-73 0.07922234218031576 1102 74-75 0.12593608499513564 1102 76-77 0.22217946104747455 1102 78-79 0.06520805541765995 1102 80-81 -0.011918088267531601 1102 82-83 0.044260936580887034 1102 84-85 0.03333913026502344 1102 86-87 0.28452546331521233 1102 88-89 0.28027918811206476 1102 90-91 0.20146373371602166 1102 92-93 0.08972145324252878 1102 94-95 0.03447142540450798 1102 96-97 0.005238134768720926 1102 98-99 -0.0818040050784532 1102 100-101 0.4528611152099664 1103 1 0.03848449098472173 1103 2 0.08376922285276578 1103 3 0.1286966532319198 1103 4 0.06974578339242754 1103 5 -0.08828948793139801 1103 6 -0.03945796063202778 1103 7 0.08579187747614725 1103 8 0.09962425086193605 1103 9 0.11543523061112637 1103 10-11 0.005518316106005727 1103 12-13 0.12827144374015376 1103 14-15 0.10322852278996919 1103 16-17 0.11821673760618978 1103 18-19 0.08483968015888621 1103 20-21 0.1553336691933751 1103 22-23 0.19636053198758674 1103 24-25 0.04318478167911621 1103 26-27 0.049379592162331676 1103 28-29 -0.033259708942132704 1103 30-31 0.10531297958621622 1103 32-33 0.07695521005025796 1103 34-35 0.050051658153002165 1103 36-37 0.01166944494215727 1103 38-39 0.08829641131725907 1103 40-41 0.22059868974153574 1103 42-43 0.29667764283009035 1103 44-45 0.04002249474027764 1103 46-47 -0.12940248783204567 1103 48-49 0.17382618161871477 1103 50-51 0.18441865410400027 1103 52-53 0.22397024283243638 1103 54-55 0.15824238136212188 1103 56-57 -0.015636365147074116 1103 58-59 0.24029181939232558 1103 60-61 0.21064212499114632 1103 62-63 0.030679328256155713 1103 64-65 -0.04585512292123184 1103 66-67 0.17789100901580923 1103 68-69 0.040870066115459736 1103 70-71 0.08550441736246484 1103 72-73 0.19518369934973379 1103 74-75 0.342156906112038 1103 76-77 0.33015451791332495 1103 78-79 0.32083107159956015 1103 80-81 0.36708435934237116 1103 82-83 0.31980040537169785 1103 84-85 0.41319521426214934 1103 86-87 0.4047584619400517 1103 88-89 0.42954360782094625 1103 90-91 0.16980480772378925 1103 92-93 0.2404535740143494 1103 94-95 0.3176123229767889 1103 96-97 0.26302681003229367 1103 98-99 0.18488774310313616 1103 100-101 0.5037938881011712 1104 1 0.12314834876644198 1104 2 0.08801334944970307 1104 3 0.016000000486485533 1104 4 -0.002561444558445203 1104 5 -0.023313696084557023 1104 6 0.023777061728175397 1104 7 -0.05625639099412183 1104 8 -0.023356863082696577 1104 9 0.06946253100166189 1104 10-11 -0.011780965657187892 1104 12-13 -0.023639953187320373 1104 14-15 -0.0030557412944958173 1104 16-17 0.01946379628478212 1104 18-19 0.024473293148751907 1104 20-21 -0.1197307010866524 1104 22-23 -0.09691200377024956 1104 24-25 0.0714251630961229 1104 26-27 -0.07100853906680271 1104 28-29 0.03619520722250513 1104 30-31 -0.014767775855993648 1104 32-33 -0.125613441315366 1104 34-35 -0.02531054016905898 1104 36-37 -0.11358064437545323 1104 38-39 -0.07461953156875012 1104 40-41 -0.06222830512116673 1104 42-43 0.0420993451622067 1104 44-45 0.1294636128736002 1104 46-47 0.21662072242776986 1104 48-49 0.30835821892267745 1104 50-51 0.1519886606334282 1104 52-53 0.1098288006917656 1104 54-55 0.07792582742045084 1104 56-57 -0.014104414080811978 1104 58-59 -0.08533525185232804 1104 60-61 -0.11662038255465745 1104 62-63 1.4533212453216038E-4 1104 64-65 0.06472285443521031 1104 66-67 0.020071097963835882 1104 68-69 -0.010908894350720288 1104 70-71 -0.2666487531048176 1104 72-73 -0.36028685047717346 1104 74-75 -0.07580578992472198 1104 76-77 -0.047008200298844116 1104 78-79 -0.20386740445715645 1104 80-81 -0.22950093589853893 1104 82-83 -0.0990363713096265 1104 84-85 -0.015025147689776475 1104 86-87 0.11080310842709906 1104 88-89 0.1833189709432439 1104 90-91 0.04610915287168993 1104 92-93 -0.024946938483953573 1104 94-95 -0.07005014268212406 1104 96-97 -0.05589836028715567 1104 98-99 -0.08618228190579558 1104 100-101 0.4805512891434205 1105 1 -0.14094527465194062 1105 2 -0.02818395806593088 1105 3 0.002262933694751723 1105 4 0.04885096939415945 1105 5 -0.10502218052616286 1105 6 -0.012207050622500049 1105 7 0.010207691547130082 1105 8 -0.06027332282874198 1105 9 0.05671081326602234 1105 10-11 -0.0392020975799241 1105 12-13 -0.030147914679204746 1105 14-15 0.008877662921868534 1105 16-17 -0.017237846124253053 1105 18-19 -0.0343954908004207 1105 20-21 -0.05821906160073098 1105 22-23 -0.06831961545118048 1105 24-25 -0.07854865729570548 1105 26-27 -0.09023328538157926 1105 28-29 -0.18585003049797422 1105 30-31 -0.09328612246115853 1105 32-33 -0.08360721602940657 1105 34-35 -0.0856597568687576 1105 36-37 0.03623376950648094 1105 38-39 -0.09964514903809629 1105 40-41 -0.05304375976298559 1105 42-43 -0.1813673007320986 1105 44-45 -0.2008270771437779 1105 46-47 -0.22826799014583088 1105 48-49 -0.11374218870827235 1105 50-51 0.017966332479268488 1105 52-53 -0.05938663017960977 1105 54-55 -0.07495545528399106 1105 56-57 0.10831095190368956 1105 58-59 -0.17615801429888478 1105 60-61 -0.12022088173392831 1105 62-63 -0.18911275705316655 1105 64-65 -0.20505760626165426 1105 66-67 -0.25027431225296226 1105 68-69 0.08086187507278453 1105 70-71 -0.08826242881817592 1105 72-73 -0.0632976660281841 1105 74-75 0.0023580076924503146 1105 76-77 -0.15687301394179087 1105 78-79 0.004804952125166295 1105 80-81 0.10996867808876232 1105 82-83 0.1920262638044541 1105 84-85 0.18841731017390373 1105 86-87 0.30536015909347825 1105 88-89 0.16302736544263396 1105 90-91 0.056971281097158766 1105 92-93 -3.8107026857048254E-4 1105 94-95 0.1674896487161881 1105 96-97 0.14792313975620885 1105 98-99 0.13292859114398325 1105 100-101 0.45541123627024405 1106 1 -0.3510872453572951 1106 2 -0.1934834473090774 1106 3 -0.24484207397363633 1106 4 -0.031083180962525603 1106 5 -0.13881936925292848 1106 6 -0.09722062859382419 1106 7 -0.10417088762017812 1106 8 -0.1084927090762946 1106 9 -0.32856588186982805 1106 10-11 -0.4928611146192239 1106 12-13 -0.3157637073372541 1106 14-15 -0.1639354394686947 1106 16-17 -0.1830227654302945 1106 18-19 -0.22238355907018104 1106 20-21 -0.20894157852043094 1106 22-23 -0.25418304779348233 1106 24-25 -0.18438648144338288 1106 26-27 -0.07365831106012877 1106 28-29 -0.13894623007304574 1106 30-31 -0.0121911610845018 1106 32-33 -0.10401638432590232 1106 34-35 -0.3069314429291765 1106 36-37 -0.107158049941674 1106 38-39 -0.21108300695482995 1106 40-41 -0.29435878276814975 1106 42-43 -0.29697000788409156 1106 44-45 -0.005248008325267506 1106 46-47 0.11585971313680687 1106 48-49 -0.10627078687068092 1106 50-51 -0.07732569943248535 1106 52-53 0.0240088475820599 1106 54-55 -0.17836612911720096 1106 56-57 0.06264534990005899 1106 58-59 -0.06417844457283195 1106 60-61 -0.05885970769670479 1106 62-63 0.015555813812582642 1106 64-65 -0.08338350571430908 1106 66-67 0.048194824244347956 1106 68-69 0.04517221262256044 1106 70-71 -0.09456598441750685 1106 72-73 0.12354366165821773 1106 74-75 -0.2803803806797376 1106 76-77 -0.40727050377590146 1106 78-79 -0.16819106594127575 1106 80-81 0.008886661513784588 1106 82-83 -0.1118388851311174 1106 84-85 -0.23109979244179257 1106 86-87 0.10404738735816821 1106 88-89 0.11364716817511322 1106 90-91 -0.24061141789399088 1106 92-93 -0.3217357719049616 1106 94-95 -0.7742798493913376 1106 96-97 -0.46328959117483137 1106 98-99 -0.32754755243075095 1106 100-101 0.14876717610506063 1107 1 -0.012060004209544672 1107 2 0.05697254367297688 1107 3 -0.004282593578778915 1107 4 -0.06467150408786893 1107 5 -0.12161933463595886 1107 6 -0.06594704167290644 1107 7 -0.015548150184443443 1107 8 0.005915291388184585 1107 9 -0.13308624508513134 1107 10-11 -0.049893125460030774 1107 12-13 -0.04595617113457706 1107 14-15 -0.044557274233994804 1107 16-17 -0.2790826747031545 1107 18-19 -0.12077069294912945 1107 20-21 -0.03412586460562039 1107 22-23 -0.078633366412042 1107 24-25 -0.052599884127516816 1107 26-27 -0.154859761029293 1107 28-29 -0.13762070673780613 1107 30-31 -0.09696495121582416 1107 32-33 -0.1523382827382278 1107 34-35 -0.08110718103205983 1107 36-37 -0.29181330980449616 1107 38-39 -0.07107816496049679 1107 40-41 0.04048758250445417 1107 42-43 0.08639325014271293 1107 44-45 0.13090186125857173 1107 46-47 0.2156738112277239 1107 48-49 0.11607753656139863 1107 50-51 0.2402647620577767 1107 52-53 0.02682695401173163 1107 54-55 0.137427207553678 1107 56-57 0.14216031790674322 1107 58-59 0.11968971734258105 1107 60-61 0.08552483867305938 1107 62-63 0.02468658819208258 1107 64-65 0.06336507318226126 1107 66-67 -0.16755874672647764 1107 68-69 -0.009313369298624252 1107 70-71 -0.21516888939500944 1107 72-73 -0.060815820270299525 1107 74-75 -0.0013314272406645955 1107 76-77 0.06446681912705898 1107 78-79 0.11989006923252532 1107 80-81 0.282629687453511 1107 82-83 0.38799460284660725 1107 84-85 0.2849461604944601 1107 86-87 0.24161096592062137 1107 88-89 0.2080101884841028 1107 90-91 -0.016478019854670123 1107 92-93 0.11440303339318803 1107 94-95 -0.0460132285885102 1107 96-97 -0.06778026704932216 1107 98-99 0.1883817325425241 1107 100-101 0.5800464594898997 1108 1 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209.0 9 469.0 10 800.0 11 1332.0 12 2207.0 13 3209.0 14 4204.0 15 6220.0 16 9169.0 17 13564.0 18 11865.0 19 6154.0 20 4793.0 21 4667.0 22 4739.0 23 4975.0 24 5606.0 25 6707.0 26 7956.0 27 9709.0 28 11867.0 29 15244.0 30 18948.0 31 23887.0 32 30907.0 33 41230.0 34 57647.0 35 85266.0 36 133798.0 37 213548.0 38 187965.0 39 19334.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.23447174335717 19.356662773855078 15.273324956931155 21.1355405258566 2 15.70028417328771 20.791256530976998 40.099328844906765 23.40913045082853 3 19.421847073647804 25.889293679121433 30.35497935921296 24.333879888017798 4 12.687309211115974 17.14487565441576 37.24568072419374 32.92213441027452 5 13.030877953042394 38.40956236810772 33.435384516413436 15.124175162436446 6 31.023182002709927 36.8982406562874 17.198125194413542 14.880452146589128 7 28.282992307813977 34.2250083037649 19.925134310102123 17.566865078319 8 27.7086353882735 34.044381646307066 19.10424783706695 19.14273512835248 9 27.394621269237057 16.329788636231832 20.11061142070827 36.164978673822844 10-11 24.944351594586472 27.60319075461452 26.266680022986932 21.185777627812076 12-13 26.479456771090636 24.82549577968417 27.734980299568875 20.960067149656318 14-15 23.134816236364692 25.508480384662025 27.131853242158872 24.224850136814414 16-17 21.344999024637136 29.243540198130468 27.572506366219756 21.838954411012637 18-19 21.28157407749126 26.398696704327975 31.729608325908277 20.59012089227249 20-21 22.12781998492142 25.37008430298461 32.08200429159659 20.420091420497382 22-23 21.647947420561174 25.640279409989187 32.00023619834545 20.71153697110419 24-25 21.43126403402911 25.736781585561246 32.144233572640054 20.687720807769587 26-27 21.283330828990273 26.066836961994618 31.923256384097687 20.726575824917422 28-29 20.93920184662139 26.26474109918894 32.09153176674911 20.70452528744056 30-31 21.123459849319616 25.8709990351816 32.15423386565299 20.85130724984579 32-33 20.824682479846896 25.99009874889942 31.969494867482457 21.215723903771227 34-35 21.347635140478612 25.87416237419137 31.922044782335917 20.8561577029941 36-37 21.448071154038793 25.772619191977775 31.70598872796866 21.073320926014773 38-39 21.28758442160982 25.97470383238521 31.82920078239918 20.908510963605785 40-41 21.272874895214393 26.02763703848202 31.76034543661987 20.93914262968372 42-43 21.290055525381902 25.98772430303254 31.497128815722174 21.225091355863384 44-45 21.37679058168532 26.26114417971983 31.098047692607807 21.26401754598704 46-47 21.495573961502163 26.743764267976992 30.42541637449716 21.335245396023684 48-49 21.728448434938024 26.962192826601573 29.883536402123656 21.425822336336747 50-51 21.841274192953136 27.07512402925034 29.663526173994192 21.42007560380233 52-53 21.98568061874911 27.216894339204845 29.203207625755905 21.59421741629014 54-55 21.594164693973312 27.22327373954121 29.432655148597853 21.749906417887626 56-57 21.735829559294153 27.59101189942691 29.166987394094047 21.50617114718489 58-59 21.935330806176946 26.96878311620526 29.48590468859564 21.60998138902216 60-61 21.708203065275498 27.013702530143984 29.855329962619876 21.422764441960638 62-63 21.731769940898282 27.140183368217937 29.86007497113453 21.267971719749255 64-65 21.50632931413538 27.08914816552699 29.714930432902943 21.68959208743469 66-67 21.429987399366276 27.492948390124056 29.412726112836303 21.664338097673365 68-69 21.674935283356092 27.16090323873192 29.609485799243963 21.554675678668023 70-71 21.570176039815895 27.45219403921486 29.37260442972906 21.605025491240188 72-73 21.58271674453769 27.377544252569958 29.08242940730501 21.957309595587343 74-75 21.473852366968412 27.435006563928443 29.07219266843462 22.01894840066852 76-77 21.480073600354295 27.344007845080743 29.317931387176877 21.857987167388085 78-79 21.474590479404025 27.33694305462559 29.152594201599598 22.035872264370784 80-81 21.348215085963737 27.665403088473322 29.00702788483337 21.97935394072957 82-83 21.44349942321603 27.51691864498999 28.993780855833535 22.045801075960444 84-85 21.30950391284244 27.25418792504678 28.991043932550326 22.44526422956045 86-87 21.367827787824307 27.14044697980208 28.916450944520307 22.575274287853304 88-89 21.133898868051855 27.02398338192574 29.048467625861353 22.793650124161054 90-91 20.964835916940018 26.957003631409076 29.300520060806463 22.777640390844443 92-93 20.969514963085906 26.745807589071763 29.491996893553598 22.792680554288737 94-95 20.658659904150827 26.847363620546943 29.482952238853184 23.011024236449046 96-97 20.762206534403948 26.823269521755865 29.24638720323926 23.16813674060093 98-99 20.626955347172647 26.86814409280854 29.151030040248386 23.353870519770425 100-101 20.179999757441152 26.879633335389812 29.3461931707029 23.59417373646614 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 343.0 1 273.5 2 325.0 3 455.5 4 368.5 5 319.5 6 660.5 7 1448.5 8 2381.0 9 2807.0 10 2369.5 11 1899.5 12 1910.5 13 2051.0 14 2227.5 15 2311.0 16 2373.0 17 2497.5 18 2614.5 19 2715.5 20 2854.5 21 3121.0 22 3464.0 23 3922.0 24 4489.5 25 5266.5 26 6275.5 27 7530.0 28 8805.0 29 10548.0 30 12907.0 31 14519.5 32 16148.0 33 17759.0 34 19391.0 35 20996.5 36 23144.0 37 25531.0 38 26361.5 39 26651.0 40 27647.5 41 29627.0 42 31054.5 43 32468.5 44 34256.0 45 35556.5 46 35354.0 47 34807.5 48 36845.0 49 37022.0 50 34745.5 51 32793.5 52 30450.0 53 28985.0 54 27874.0 55 25797.0 56 23158.0 57 20904.5 58 19184.0 59 17251.5 60 14617.5 61 11669.0 62 9238.5 63 6959.0 64 4805.0 65 3537.5 66 2664.5 67 2049.5 68 1478.0 69 925.0 70 646.5 71 496.5 72 393.5 73 337.5 74 278.5 75 230.5 76 167.0 77 91.5 78 53.5 79 32.0 80 18.0 81 12.0 82 8.5 83 5.0 84 2.0 85 0.0 86 1.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04892631001776742 2 0.0 3 0.0 4 0.0 5 0.015605805781529264 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0037432844948938437 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010017240197603243 24-25 5.272231682949075E-5 26-27 0.001318057920737269 28-29 0.0040596183958707885 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.015816695048847227 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.10889267317963E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.1633390097694455E-4 84-85 0.0014235025543962504 86-87 0.0 88-89 0.0 90-91 0.011651632019317456 92-93 0.0015289471880552319 94-95 0.0 96-97 0.0 98-99 4.21778534635926E-4 100-101 0.014920415662745884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 948365.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.961952072193455 #Duplication Level Percentage of deduplicated Percentage of total 1 78.84754177657895 44.91309895687761 2 12.048727329460222 13.726380573432815 3 3.7928171611226316 6.481388080513806 4 1.6865733731016994 3.842820465794266 5 0.8869764065924811 2.5261953780743642 6 0.5784165333067476 1.976864090878995 7 0.38576290377268435 1.5381665625150875 8 0.2594299782621774 1.182211039028827 9 0.19984981985179148 1.024545227403082 >10 1.1795590014847894 12.235732110809503 >50 0.09365551426927458 3.6536169625295147 >100 0.037903202133735305 3.8930460963675855 >500 0.0018580000418935297 0.5793424833078515 >1k 5.57400012568059E-4 0.8059358805972395 >5k 3.7160000837870597E-4 1.6206560918694064 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8654 0.9125178596848259 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6659 0.7021558155351578 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4598 0.48483442556399703 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1626 0.17145297432950396 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1391 0.14667348541964328 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0544463365898152E-4 0.0 10-11 0.0 0.0 0.0 1.0544463365898152E-4 0.0 12-13 0.0 0.0 0.0 2.1088926731796303E-4 0.0 14-15 0.0 0.0 0.0 2.1088926731796303E-4 0.0 16-17 0.0 0.0 0.0 2.636115841474538E-4 0.0 18-19 0.0 0.0 0.0 3.1633390097694455E-4 0.0 20-21 0.0 0.0 0.0 3.690562178064353E-4 0.0 22-23 0.0 0.0 0.0 4.2177853463592607E-4 0.0 24-25 0.0 0.0 0.0 7.908347524423614E-4 0.0 26-27 0.0 0.0 0.0 0.0014762248712257412 0.0 28-29 0.0 0.0 0.0 0.002583393524645047 0.0 30-31 0.0 0.0 0.0 0.004270507663188751 0.0 32-33 0.0 0.0 0.0 0.0074865689897876874 0.0 34-35 0.0 0.0 0.0 0.010755352633216114 0.0 36-37 0.0 0.0 0.0 0.01660752980128959 0.0 38-39 0.0 0.0 0.0 0.025570323662303017 0.0 40-41 0.0 0.0 0.0 0.034849451424293396 0.0 42-43 0.0 0.0 0.0 0.0485045314831315 0.0 44-45 0.0 0.0 0.0 0.059312606433177095 0.0 46-47 0.0 0.0 0.0 0.0849356524123096 0.0 48-49 0.0 0.0 0.0 0.11398564898535901 0.0 50-51 0.0 0.0 0.0 0.1300132333015242 0.0 52-53 0.0 0.0 0.0 0.16243745815166102 0.0 54-55 0.0 0.0 0.0 0.21805950240677374 0.0 56-57 0.0 0.0 0.0 0.29930459264101905 0.0 58-59 0.0 0.0 0.0 0.3864545823601673 0.0 60-61 0.0 0.0 0.0 0.5147807015231478 0.0 62-63 0.0 0.0 0.0 0.6343021937756033 0.0 64-65 0.0 0.0 0.0 0.7564070795527038 1.0544463365898152E-4 66-67 0.0 0.0 0.0 0.9314978937434426 1.0544463365898152E-4 68-69 0.0 0.0 0.0 1.1682738186246857 1.0544463365898152E-4 70-71 0.0 0.0 0.0 1.3124693551533428 1.0544463365898152E-4 72-73 0.0 0.0 0.0 1.508965429976855 1.0544463365898152E-4 74-75 0.0 0.0 0.0 1.727710322502412 1.0544463365898152E-4 76-77 0.0 0.0 0.0 1.951358390493112 1.0544463365898152E-4 78-79 0.0 0.0 0.0 2.2030020087202713 1.0544463365898152E-4 80-81 0.0 0.0 0.0 2.601899057852198 1.0544463365898152E-4 82-83 0.0 0.0 0.0 3.0517258650414134 1.0544463365898152E-4 84-85 0.0 0.0 0.0 3.5995634592166517 1.0544463365898152E-4 86-87 0.0 0.0 0.0 4.300822995365708 1.0544463365898152E-4 88-89 0.0 0.0 0.0 5.099038872164199 1.0544463365898152E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3910 0.0 45.21159 1 GTATCAA 6705 0.0 38.697014 1 CGTAGAC 60 0.003948267 31.6705 3 TATCAAC 8535 0.0 30.501638 2 ATCAACG 8585 0.0 30.102654 3 TCAACGC 8720 0.0 29.636614 4 CAACGCA 8855 0.0 29.29208 5 CGGTATA 65 0.005840109 29.234306 2 AACGCAG 9060 0.0 28.780525 6 GTACATG 6045 0.0 27.671328 1 GTGTACG 105 6.88405E-5 27.154734 1 TACATGG 6200 0.0 26.281406 2 ACATGGG 6125 0.0 25.284693 3 ACGCAGA 10325 0.0 25.162386 7 CGCAGAG 10365 0.0 25.019457 8 GTACGCT 95 0.0012255317 25.003025 3 GAGTACT 6350 0.0 23.63567 12-13 GCAGAGT 10930 0.0 23.508863 9 TACCTGG 675 0.0 23.225033 2 ATGGGAG 1675 0.0 22.97293 5 >>END_MODULE