FastQCFastQC Report
Thu 26 May 2016
SRR937192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161537
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65740.5659742220867695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46480.400159443909234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31370.2700731875093088No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17200.14807965652407112No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.10064251074223206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41100.041.8486181
GTATCAA68700.037.2774661
ATCAACG85950.029.685393
TATCAAC86500.029.5502932
TCAACGC87450.029.1231334
GTACATG76500.028.8805771
CAACGCA88900.028.6456495
AACGCAG90950.028.0485886
TACATGG77800.027.4807472
ACATGGG78000.026.741413
GTGTAGC5650.026.0691951
GTGTACG1501.0575568E-625.340381
ACGCAGA103300.024.3273477
CATGGGG25300.024.227154
CGCAGAG104300.024.1396478
CATGGGA54650.023.475064
GAGTACT51550.023.082412-13
GCAGAGT108700.022.7682089
TAGCGCG1302.9351452E-421.930124
TAAGGTG5650.021.8635435
Produced by FastQC (version 0.11.5)