##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937192_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1161537 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85567915615258 33.0 31.0 34.0 30.0 34.0 2 31.936934423957222 33.0 31.0 34.0 30.0 34.0 3 31.98054646558827 33.0 31.0 34.0 30.0 34.0 4 35.597734725626474 37.0 35.0 37.0 33.0 37.0 5 35.61744223386771 37.0 35.0 37.0 33.0 37.0 6 35.6589251999721 37.0 35.0 37.0 33.0 37.0 7 35.54839751122866 37.0 35.0 37.0 33.0 37.0 8 35.554605664735604 37.0 35.0 37.0 33.0 37.0 9 37.18821526993975 39.0 37.0 39.0 34.0 39.0 10-11 37.08674239391427 39.0 37.0 39.0 33.0 39.0 12-13 36.93706098040786 39.0 37.0 39.0 33.0 39.0 14-15 38.12132932485147 40.0 38.0 41.0 33.0 41.0 16-17 38.06871111294776 40.0 38.0 41.0 33.0 41.0 18-19 38.05545453997591 40.0 38.0 41.0 33.0 41.0 20-21 37.96093753363001 40.0 38.0 41.0 33.0 41.0 22-23 37.815587880541045 40.0 38.0 41.0 33.0 41.0 24-25 37.7497643208955 40.0 37.5 41.0 32.5 41.0 26-27 37.76240188646595 40.0 37.5 41.0 33.0 41.0 28-29 37.67781783963834 40.0 37.0 41.0 33.0 41.0 30-31 37.455523155956286 40.0 37.0 41.0 32.0 41.0 32-33 37.22349352625014 39.5 36.5 41.0 32.0 41.0 34-35 37.26237132351359 40.0 37.0 41.0 32.0 41.0 36-37 37.143116405245806 40.0 36.0 41.0 31.5 41.0 38-39 37.02705165655506 40.0 36.0 41.0 31.0 41.0 40-41 36.80511124484197 39.5 36.0 41.0 30.5 41.0 42-43 36.52529364109796 39.0 36.0 41.0 30.0 41.0 44-45 36.26164513054685 39.0 35.0 41.0 30.0 41.0 46-47 35.869606822684084 39.0 35.0 41.0 28.5 41.0 48-49 35.50970438307174 39.0 35.0 40.5 27.0 41.0 50-51 34.509353985279844 38.0 34.0 39.5 25.0 40.5 52-53 34.61496017776446 38.0 34.5 39.5 26.0 40.5 54-55 34.98193729515289 39.0 35.0 40.0 26.0 41.0 56-57 34.77657620893695 38.0 34.5 40.0 25.5 41.0 58-59 34.511320775834086 38.0 34.0 40.0 24.5 41.0 60-61 34.20944963440682 38.0 34.0 40.0 23.5 41.0 62-63 33.894141986006474 37.5 34.0 40.0 22.5 41.0 64-65 33.47173271277626 37.0 33.0 40.0 22.0 41.0 66-67 33.11832081113215 36.0 33.0 39.0 20.5 41.0 68-69 32.64922253875683 36.0 32.0 39.0 19.0 40.5 70-71 32.1914713005268 35.0 32.0 38.5 17.5 40.0 72-73 31.74057563383689 35.0 31.0 38.0 14.0 40.0 74-75 31.260744169148293 35.0 31.0 37.0 9.5 39.0 76-77 30.780992770785605 35.0 30.5 36.5 7.0 39.0 78-79 30.294904079680627 34.0 30.0 36.0 4.0 38.5 80-81 29.81946894502715 34.0 29.5 35.5 2.0 37.5 82-83 29.357020482343653 34.0 29.0 35.0 2.0 37.0 84-85 28.85924081626328 34.0 29.0 35.0 2.0 36.5 86-87 28.416181318373845 34.0 28.5 35.0 2.0 36.0 88-89 27.982688024574337 34.0 27.0 35.0 2.0 36.0 90-91 27.49879513093427 33.0 26.5 35.0 2.0 35.0 92-93 27.009021236516787 33.0 25.5 35.0 2.0 35.0 94-95 26.481863685788745 33.0 25.0 35.0 2.0 35.0 96-97 25.78199704357244 32.0 23.5 35.0 2.0 35.0 98-99 24.89486731804497 32.0 17.5 34.0 2.0 35.0 100-101 22.370294273880212 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.007795405525968135 1101 2 -0.05240875073479856 1101 3 -0.20162845835242393 1101 4 -0.1604629940124198 1101 5 -0.11073189816298878 1101 6 -0.1718522639452118 1101 7 -0.220281600526107 1101 8 -0.11295502504589905 1101 9 -0.12691946300845558 1101 10-11 -0.294596981187631 1101 12-13 -0.17945077901037365 1101 14-15 -0.20401164039705577 1101 16-17 -0.3347931813080507 1101 18-19 -0.10169534347470233 1101 20-21 -0.10704706023618371 1101 22-23 -0.3124302792288418 1101 24-25 -0.19770191074082533 1101 26-27 -0.2846532360866476 1101 28-29 -0.33703235274476384 1101 30-31 -0.3022718459470326 1101 32-33 -0.34517849609053997 1101 34-35 -0.33045740878402796 1101 36-37 -0.4021750446687591 1101 38-39 -0.4914187791582165 1101 40-41 -0.6390251388998252 1101 42-43 -0.5311312059040105 1101 44-45 -0.4419674792714616 1101 46-47 -0.25699894925041633 1101 48-49 -0.31283017256039614 1101 50-51 -0.3489795698034186 1101 52-53 -0.43919073590272006 1101 54-55 -0.4889027081347024 1101 56-57 -0.42988683651007875 1101 58-59 -0.6643420215114233 1101 60-61 -0.5003747961401714 1101 62-63 -0.5568881304627809 1101 64-65 -0.5997881192887249 1101 66-67 -0.6505017530819472 1101 68-69 -0.548703424703703 1101 70-71 -0.4943141759776992 1101 72-73 -0.5058623798910666 1101 74-75 -0.4739743709740871 1101 76-77 -0.49515394834706683 1101 78-79 -0.6138672739978972 1101 80-81 -0.5044985633536214 1101 82-83 -0.44296284595217017 1101 84-85 -0.4880598505438023 1101 86-87 -0.4696563038869961 1101 88-89 -0.671856093391451 1101 90-91 -0.7617135485461723 1101 92-93 -0.6166561251105129 1101 94-95 -0.552091556474938 1101 96-97 -0.4157596171342526 1101 98-99 -0.4683000476295369 1101 100-101 0.010765389255578128 1102 1 0.04730901220011674 1102 2 0.1157777881777271 1102 3 0.10187525543836173 1102 4 0.014493305636989362 1102 5 0.0764593172289878 1102 6 0.0075495487078853785 1102 7 0.10362638338506969 1102 8 0.12868357551811727 1102 9 0.1369238424131467 1102 10-11 0.09659318579007703 1102 12-13 0.10939523612857016 1102 14-15 0.21037370702812552 1102 16-17 0.18936941749809932 1102 18-19 0.09120480294857458 1102 20-21 0.21617590713093193 1102 22-23 0.1133488876759472 1102 24-25 0.05981169204023473 1102 26-27 -0.06953301331832051 1102 28-29 0.014060500893052108 1102 30-31 -0.004247476227291713 1102 32-33 -0.020449911233036744 1102 34-35 0.2523489912727257 1102 36-37 0.10659695084592613 1102 38-39 0.20167922589860865 1102 40-41 0.34836787653275536 1102 42-43 0.21958563750191473 1102 44-45 0.5268561880895462 1102 46-47 0.5442041652494254 1102 48-49 0.4887227418145059 1102 50-51 0.44825789800022164 1102 52-53 0.42525639307786633 1102 54-55 0.505239966858845 1102 56-57 0.5475076175360698 1102 58-59 0.1964468905222816 1102 60-61 0.3922405836242504 1102 62-63 0.2266748226758608 1102 64-65 0.31026503058524213 1102 66-67 0.2610798744432614 1102 68-69 0.2530964188224445 1102 70-71 0.22392586753542787 1102 72-73 0.34900653876420407 1102 74-75 0.41524351591935726 1102 76-77 0.4232303506638395 1102 78-79 0.35230224986042913 1102 80-81 0.3223621256437461 1102 82-83 0.34046176379926507 1102 84-85 0.38852562970679116 1102 86-87 0.31191426799145816 1102 88-89 -0.006009567840294494 1102 90-91 0.1524654662203062 1102 92-93 -0.09267778708362329 1102 94-95 0.03470748891627551 1102 96-97 -0.07554347037201836 1102 98-99 -0.13168082893465183 1102 100-101 0.17515812681896037 1103 1 -0.0175721844129626 1103 2 -0.0678620342699574 1103 3 -0.1451462671846393 1103 4 -0.1517120731537176 1103 5 -0.16636958351329412 1103 6 -0.13218710689284308 1103 7 -0.129981733292162 1103 8 -0.09065321436587936 1103 9 -0.009870144680739656 1103 10-11 -0.0027913492541244977 1103 12-13 -0.07268913852173142 1103 14-15 -0.06615283256678595 1103 16-17 -0.1449474164009601 1103 18-19 -0.17645531119219982 1103 20-21 -0.0546473238735814 1103 22-23 -0.1823055567270444 1103 24-25 -0.18464725831226048 1103 26-27 -0.2919508580719494 1103 28-29 -0.27309306693864244 1103 30-31 -0.21103251110363885 1103 32-33 -0.293343342543416 1103 34-35 -0.0806212799048609 1103 36-37 -0.21150890912647924 1103 38-39 -0.30706410656437555 1103 40-41 -0.3949422059022467 1103 42-43 -0.40677118296881787 1103 44-45 -0.44879751029532855 1103 46-47 -0.19715880987087075 1103 48-49 -0.250644634812744 1103 50-51 -0.42030489807572735 1103 52-53 -0.41250331448303257 1103 54-55 -0.31356715015409975 1103 56-57 -0.28370951104015063 1103 58-59 -0.28759205162401713 1103 60-61 -0.2202029987548002 1103 62-63 -0.18192303625211537 1103 64-65 -0.33649538569287074 1103 66-67 -0.13978550658548983 1103 68-69 -0.26578156991268287 1103 70-71 -0.3234686641516866 1103 72-73 -0.30851891910442575 1103 74-75 -0.34427754044231307 1103 76-77 -0.3202699663938695 1103 78-79 -0.22259424012428042 1103 80-81 -0.29318489214878696 1103 82-83 -0.3925942994630738 1103 84-85 -0.2982474688795058 1103 86-87 -0.2455701588801169 1103 88-89 -0.10489427073857271 1103 90-91 -0.024258768178587076 1103 92-93 -0.07912636778413429 1103 94-95 -0.48803114363249733 1103 96-97 -0.19241467034217763 1103 98-99 0.13091382211537095 1103 100-101 0.5627742258901698 1104 1 0.09346811434607716 1104 2 -8.413814048964241E-4 1104 3 0.02296884131613197 1104 4 -0.017869927163793875 1104 5 -0.013578602632144054 1104 6 -0.026768456117508777 1104 7 0.010691106031103459 1104 8 -0.003409104272314778 1104 9 0.08891988703423692 1104 10-11 -0.05250210616314632 1104 12-13 -0.030788357961277768 1104 14-15 0.10089798571257802 1104 16-17 -0.09061178172016326 1104 18-19 -0.0513460769322549 1104 20-21 0.0343547352438307 1104 22-23 -0.03414091088406934 1104 24-25 -0.08646539243808604 1104 26-27 -0.08051194837923248 1104 28-29 -0.08271238523914093 1104 30-31 -0.17158843880184804 1104 32-33 -0.03414519851789066 1104 34-35 0.01660437633862699 1104 36-37 9.236133676466807E-4 1104 38-39 0.093733455331801 1104 40-41 0.25732901663253216 1104 42-43 0.20106920167178544 1104 44-45 0.20693860596344393 1104 46-47 0.31500523557835436 1104 48-49 0.4353334022397135 1104 50-51 0.3473206174929757 1104 52-53 0.405640769331157 1104 54-55 0.4340437830355839 1104 56-57 0.45251847148625046 1104 58-59 0.47134402732874037 1104 60-61 0.5199649463392859 1104 62-63 0.7116197677907294 1104 64-65 0.7407562487528239 1104 66-67 0.5746075249609248 1104 68-69 0.6286042627377597 1104 70-71 0.7364905615346728 1104 72-73 0.8753344235489386 1104 74-75 0.8519691242392327 1104 76-77 0.774094104411791 1104 78-79 0.989772462504483 1104 80-81 1.178993220778672 1104 82-83 1.1472009740391123 1104 84-85 1.2110681065294173 1104 86-87 1.344997490097441 1104 88-89 1.2010282056039223 1104 90-91 1.434300218807536 1104 92-93 1.3960362267135196 1104 94-95 1.2839073947001687 1104 96-97 1.3850956288880454 1104 98-99 1.6620245165562366 1104 100-101 1.828427574275441 1105 1 0.10092003719061893 1105 2 0.12455219245358151 1105 3 -0.07034961105043536 1105 4 -0.020057603287192194 1105 5 0.047156719491802335 1105 6 0.02731925007072533 1105 7 -0.030770818447187764 1105 8 -0.011991600349865905 1105 9 0.0018091952626235752 1105 10-11 0.08267679895474345 1105 12-13 0.10615625216738067 1105 14-15 0.09162646349408021 1105 16-17 -0.02669210027210056 1105 18-19 0.04136527839256132 1105 20-21 -0.06327188267461992 1105 22-23 -0.045343204705503126 1105 24-25 -0.07850111062519005 1105 26-27 -0.08809969468614298 1105 28-29 -0.1457223878395979 1105 30-31 0.06352294127086111 1105 32-33 -0.032747570256553615 1105 34-35 0.029708198633201732 1105 36-37 0.017533952771628947 1105 38-39 0.12332266911213452 1105 40-41 0.22068640828069164 1105 42-43 0.389036789650099 1105 44-45 0.44989391182725313 1105 46-47 0.4604682969135183 1105 48-49 0.523948302605227 1105 50-51 0.37315801022386097 1105 52-53 0.41980459466121545 1105 54-55 0.44385014034698855 1105 56-57 0.3916562680140032 1105 58-59 0.3612680531941663 1105 60-61 0.6322496226492618 1105 62-63 0.5650026903829541 1105 64-65 0.6583766603607515 1105 66-67 0.6741189955181639 1105 68-69 0.6459171622931734 1105 70-71 0.6391160145615089 1105 72-73 0.742289058512501 1105 74-75 0.6869470009656666 1105 76-77 0.4920131826296732 1105 78-79 0.47978001254890046 1105 80-81 0.632199379526817 1105 82-83 0.6825692102965313 1105 84-85 0.7099160238868798 1105 86-87 0.7577497664696686 1105 88-89 0.5599709480391333 1105 90-91 0.8210524526442988 1105 92-93 0.9718820298652027 1105 94-95 1.1401909457538366 1105 96-97 1.012849679182363 1105 98-99 1.0183100010399784 1105 100-101 1.564392080366229 1106 1 -0.12590299804059768 1106 2 -0.21901291318063087 1106 3 -0.11838672603237654 1106 4 0.07162930723972494 1106 5 0.07419965133340867 1106 6 -0.12485196363757467 1106 7 -0.3986959474719498 1106 8 -0.20539661233699036 1106 9 -0.5758195084617128 1106 10-11 -0.7195380332414345 1106 12-13 -0.23262965746912556 1106 14-15 -0.32047352790957007 1106 16-17 -0.23462334225282433 1106 18-19 -0.45059342519381573 1106 20-21 -0.24739921045322433 1106 22-23 -0.21046146811659838 1106 24-25 -0.1857489962959349 1106 26-27 -0.5111809853264191 1106 28-29 -0.18716950281093148 1106 30-31 -0.28963186403254326 1106 32-33 -0.28152285650951825 1106 34-35 -0.17340537605576145 1106 36-37 -0.35997583485621476 1106 38-39 -0.4266656037761649 1106 40-41 -0.45769967582976534 1106 42-43 -0.3798839234007332 1106 44-45 -0.25523025228439167 1106 46-47 -0.24641705650766 1106 48-49 0.15948452876761365 1106 50-51 -0.04120064411925739 1106 52-53 0.0378960788678242 1106 54-55 -0.23869376203250425 1106 56-57 -0.2566033437071624 1106 58-59 -0.12461855359524776 1106 60-61 -0.1504170164242069 1106 62-63 -0.3215592600745083 1106 64-65 -0.1457227346051866 1106 66-67 -0.14231801078899764 1106 68-69 -0.2716056896554946 1106 70-71 -0.0699478753798104 1106 72-73 -0.19030419325106962 1106 74-75 0.08717846549619424 1106 76-77 0.0779848507768186 1106 78-79 0.18729841774661438 1106 80-81 -0.07341509459447337 1106 82-83 -0.18300731255648017 1106 84-85 -0.18615656109752976 1106 86-87 -0.03553335784514289 1106 88-89 0.33255943466863513 1106 90-91 0.4834368663046362 1106 92-93 0.4537068639610631 1106 94-95 0.518832054745932 1106 96-97 0.5611253161873968 1106 98-99 0.5070421569925223 1106 100-101 0.8817447034717212 1107 1 -0.009558632561990521 1107 2 -0.2077658632356716 1107 3 -0.06098820011096606 1107 4 -0.11771677970903482 1107 5 -0.04175732754229955 1107 6 -0.0332406139915804 1107 7 0.011613448271937443 1107 8 -0.06311576486460524 1107 9 -0.13379576512375735 1107 10-11 -0.16321012332054607 1107 12-13 -0.021075545030996068 1107 14-15 0.010197778465226293 1107 16-17 -0.14989871049337467 1107 18-19 -0.23122264374460855 1107 20-21 -0.19443469819194092 1107 22-23 0.042906953288579075 1107 24-25 0.01406406454944431 1107 26-27 0.0110546707635919 1107 28-29 -0.030885801851063377 1107 30-31 -0.11079266184800218 1107 32-33 -0.049443673348214645 1107 34-35 -0.0913963578742667 1107 36-37 -0.003827161013404634 1107 38-39 -0.23146769052844007 1107 40-41 -0.027473640770715235 1107 42-43 -0.1256890958491752 1107 44-45 -0.1658597722599211 1107 46-47 -0.1920676650507147 1107 48-49 -0.18043326042708685 1107 50-51 -0.22644261003370048 1107 52-53 -0.4643679558027216 1107 54-55 -0.2973649701651624 1107 56-57 -0.3541976952309227 1107 58-59 -0.2506874950139064 1107 60-61 -0.1478738847775105 1107 62-63 -0.17729849865330038 1107 64-65 0.05404092607540889 1107 66-67 -0.0608161118131747 1107 68-69 -0.1812854426211814 1107 70-71 0.1427734206125706 1107 72-73 -0.077741033605367 1107 74-75 -0.07646418543387057 1107 76-77 -0.26786458645203837 1107 78-79 -0.09588816639665865 1107 80-81 -0.08231996271557307 1107 82-83 0.053817635587304125 1107 84-85 0.14189904841677148 1107 86-87 0.13212354489290945 1107 88-89 0.19739807196090098 1107 90-91 0.30299508928302643 1107 92-93 0.27475406930722457 1107 94-95 0.5154033483939102 1107 96-97 0.489644952334249 1107 98-99 0.8568410955308998 1107 100-101 1.1984218083652962 1108 1 0.015979785172763883 1108 2 0.08260490093713813 1108 3 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0.06996077149214841 2316 5 -0.044237508241181445 2316 6 0.027857561936741604 2316 7 0.11656874807643192 2316 8 -0.10062058919568528 2316 9 -0.27083523922421904 2316 10-11 -0.4090026739206607 2316 12-13 -0.06547125406530796 2316 14-15 0.12037126457431668 2316 16-17 -0.35291209840755045 2316 18-19 0.08529934571461695 2316 20-21 -0.27934846129672053 2316 22-23 -0.07090850734804377 2316 24-25 -0.027886262239668724 2316 26-27 0.10801423496041451 2316 28-29 0.09999592769536036 2316 30-31 -0.037439103231591275 2316 32-33 0.17608510497297658 2316 34-35 0.08774212731741216 2316 36-37 0.054900765694718245 2316 38-39 0.08275681011375013 2316 40-41 0.004657195088135779 2316 42-43 0.1604617522840428 2316 44-45 0.062028183046216157 2316 46-47 -0.23016327222045163 2316 48-49 -0.07905271086459464 2316 50-51 0.11324961346095108 2316 52-53 0.12959735962945018 2316 54-55 0.09088295932684076 2316 56-57 0.22546150136768972 2316 58-59 0.20090073804715303 2316 60-61 0.33441053880192584 2316 62-63 0.5396666965660302 2316 64-65 0.8262201950548018 2316 66-67 0.6071465336285442 2316 68-69 0.5100174747546333 2316 70-71 0.5364886617025917 2316 72-73 0.5157086320131263 2316 74-75 0.5566153828122253 2316 76-77 0.29758260531275127 2316 78-79 0.7555896606613537 2316 80-81 0.676022175681041 2316 82-83 0.5242168054860095 2316 84-85 0.6923299165432866 2316 86-87 0.5420904547269529 2316 88-89 0.543687765924318 2316 90-91 0.3225170217993316 2316 92-93 0.8080886153139417 2316 94-95 0.41787040058526514 2316 96-97 0.2646104702301457 2316 98-99 0.6574839178753074 2316 100-101 0.4032984952994241 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4459.0 3 947.0 4 703.0 5 808.0 6 845.0 7 994.0 8 1273.0 9 1582.0 10 1993.0 11 2791.0 12 3854.0 13 5126.0 14 5832.0 15 7718.0 16 10209.0 17 13816.0 18 11717.0 19 7434.0 20 7280.0 21 7407.0 22 8129.0 23 8867.0 24 10199.0 25 11557.0 26 13279.0 27 15753.0 28 18898.0 29 22468.0 30 27537.0 31 34778.0 32 43954.0 33 58505.0 34 80182.0 35 114047.0 36 167906.0 37 230271.0 38 178248.0 39 20171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.59425054134737 19.082441012770534 14.571630031682522 21.751678414199567 2 15.109027279253409 20.558234165332156 40.66080747142305 23.671931083991385 3 19.23943015498873 25.843706753450235 29.521108630156366 25.395754461404664 4 12.227475883966935 16.48898169079708 37.47131098034478 33.8122314448912 5 13.24158008176169 38.59150044263136 33.210121331034756 14.9567981445722 6 31.100909988469798 36.45002658160553 17.75176577808157 14.697297651843105 7 28.743718072532477 33.67164905612503 19.962440050367615 17.622192820974885 8 27.360717148870695 34.43365656694664 19.312812874575748 18.892813409606916 9 27.37290994863157 16.61962424161887 20.04076339026459 35.96670241948497 10-11 24.84400491728086 27.60343829947345 26.49191535338533 21.060641429860354 12-13 26.220518616040046 24.783284292086474 27.575291151388363 21.420905940485113 14-15 22.941247278128948 25.650153442986213 26.532326573615745 24.87627270526909 16-17 21.698977848199583 29.6198775903315 26.72913149891433 21.952013062554585 18-19 21.645449740586134 27.37024452857867 30.025520715356652 20.95878501547854 20-21 22.73004570670224 26.27483905148885 30.065466632036454 20.92964860977245 22-23 22.204672978642076 26.521254813578715 29.98064645833639 21.29342574944282 24-25 21.859038073939093 26.640627157017033 30.13499475413457 21.3653400149093 26-27 21.672732638205783 26.917554559229078 29.939236740286677 21.47047606227846 28-29 21.3851031533238 27.108393186452645 30.11747406173062 21.389029598492932 30-31 21.514766969383032 26.89712634478081 30.132453331526843 21.455653354309316 32-33 21.242632401039018 27.05619654984941 29.935751948152816 21.76541910095876 34-35 21.772485539554562 26.983251602257052 29.81048229102477 21.433780567163613 36-37 21.807485537449477 26.83271761344296 29.71474401425784 21.645052834849732 38-39 21.61165697768478 27.003117011982702 29.87171716082272 21.513508849509797 40-41 21.75024957096228 26.959574249626183 29.829446181111297 21.460729998300245 42-43 21.610050118577007 27.062090173747567 29.484797249928057 21.84306245774737 44-45 21.602495476754203 27.321267604843726 29.24366324871962 21.83257366968245 46-47 21.80171643001888 27.426761740043016 28.82317353135108 21.948348298587018 48-49 21.90580867776049 27.678755600204717 28.4417902358149 21.97364548621989 50-51 21.99299101415391 27.871830755561078 28.234017790447634 21.90116043983738 52-53 22.197807509919464 27.870365215034344 28.014091187871426 21.917736087174767 54-55 21.771323128335784 27.89872333961652 28.213918290973616 22.11603524107408 56-57 21.8694966776219 28.2264083543939 27.97868604497657 21.92540892300763 58-59 22.01445378180259 27.670830950107106 28.130135798602936 22.18457946948737 60-61 21.89947608929516 27.65800786254267 28.534628746332498 21.907887301829675 62-63 21.943892530133372 27.865830038383326 28.499124749697398 21.69115268178591 64-65 21.688952535412966 27.803015925050467 28.48185786504253 22.02617367449404 66-67 21.613544957477902 28.06734520282756 28.20891299059148 22.11019684910306 68-69 21.711444932115473 27.982141894296408 28.26446387939308 22.04194929419504 70-71 21.670568331765335 28.10436878650667 28.118560581061242 22.106502300666754 72-73 21.6689558069392 28.100605989495264 27.98978686838709 22.24065133517845 74-75 21.544372097357112 28.284542906270786 27.972871423974162 22.198213572397943 76-77 21.711627601964565 28.07848087722144 27.99603427983695 22.213857240977035 78-79 21.75138074353503 28.121481271845774 27.84395203701195 22.283185947607244 80-81 21.647262643675187 28.396135415749956 27.76220451788466 22.194397422690194 82-83 21.672973294751607 28.250285998250362 27.87434712164114 22.202393585356887 84-85 21.631043067105 28.03569880246211 27.75444283062567 22.57881529980722 86-87 21.818739506094015 27.904730432801898 27.8860937734576 22.390436287646484 88-89 21.660752294353696 27.76230521612264 27.86536455569734 22.711577933826327 90-91 21.415496017253748 27.895186664670586 28.120306850289033 22.56901046778663 92-93 21.6327502554893 27.800039773935648 28.128886532554016 22.438323438021037 94-95 21.386090094691436 27.71166454788947 28.077040378127315 22.825204979291776 96-97 21.521162999684634 27.74260042442914 27.949379124626773 22.78685745125946 98-99 21.413921076451416 27.80733597430019 27.70176345370125 23.076979495547143 100-101 21.411554817857564 27.5156222838664 28.289155566329626 22.78366733194641 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1922.0 1 1137.5 2 389.5 3 433.0 4 377.5 5 362.5 6 591.5 7 1072.0 8 1689.0 9 2009.0 10 1886.5 11 1738.0 12 1792.5 13 1975.0 14 2141.5 15 2182.0 16 2219.5 17 2360.0 18 2485.5 19 2617.0 20 2850.0 21 3189.0 22 3582.5 23 4135.0 24 4824.0 25 5745.0 26 6962.5 27 8628.5 28 10310.5 29 12522.5 30 15240.5 31 17127.0 32 19396.0 33 21747.5 34 24007.5 35 25929.0 36 28282.0 37 31203.5 38 32654.0 39 33078.5 40 34076.0 41 36509.5 42 38326.0 43 40221.5 44 42587.5 45 43889.5 46 43406.0 47 43238.0 48 45739.0 49 45428.0 50 42801.0 51 40639.0 52 37966.5 53 36638.5 54 35619.5 55 33136.0 56 29844.0 57 26523.5 58 24015.0 59 21541.5 60 18022.0 61 14622.0 62 11705.5 63 8743.5 64 6353.0 65 4869.5 66 3533.0 67 2629.5 68 1965.0 69 1335.0 70 952.5 71 697.0 72 535.0 73 416.0 74 320.5 75 249.5 76 177.5 77 119.0 78 81.5 79 57.5 80 45.0 81 36.5 82 28.0 83 17.5 84 9.0 85 6.0 86 5.0 87 5.0 88 4.5 89 2.0 90 3.5 91 3.0 92 2.0 93 2.0 94 0.5 95 0.0 96 0.5 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24570891844168546 2 0.22048372113845707 3 0.2139406665478586 4 0.22013934984421502 5 0.22031153549133606 6 0.24458971173539887 7 0.24588110408880645 8 0.23468903702594063 9 0.22849035372958415 10-11 0.23817579638014114 12-13 0.24265262320528747 14-15 0.2270698221408358 16-17 0.24149037008722066 18-19 0.23025525661257454 20-21 0.22607975466989 22-23 0.22177511349186466 24-25 0.21772875078452086 26-27 0.2249605479636034 28-29 0.23486122267306164 30-31 0.23688440402673355 32-33 0.23649698632071128 34-35 0.24613938255948797 36-37 0.22345392355129454 38-39 0.22164597425652388 40-41 0.21936451443217048 42-43 0.22259299531568946 44-45 0.24110295238119836 46-47 0.24562282561812493 48-49 0.24743077491289558 50-51 0.24807647108959938 52-53 0.2653811286252612 54-55 0.2487221672663032 56-57 0.22194729913898567 58-59 0.20610621960385245 60-61 0.20403999183840035 62-63 0.2100664894876358 64-65 0.20292078513211376 66-67 0.20447045595620286 68-69 0.20657973013343528 70-71 0.20804330813396385 72-73 0.2101525823111963 74-75 0.21213271725308794 76-77 0.22427180537511934 78-79 0.22039762831489654 80-81 0.21514596607770564 82-83 0.20998039666407525 84-85 0.21097046413502107 86-87 0.21790093643164185 88-89 0.21488768760702415 90-91 0.22388438766909707 92-93 0.21406980578319934 94-95 0.21148702107638412 96-97 0.22112941731516084 98-99 0.22539101208140594 100-101 0.2045135023679831 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1161537.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.548738079443226 #Duplication Level Percentage of deduplicated Percentage of total 1 78.78587919555248 45.34027926183504 2 12.064476544837015 13.885908014888232 3 3.7636687051780715 6.4978315359627015 4 1.71695426090901 3.9523420422174658 5 0.9304656929967123 2.677356322908772 6 0.5615296018094961 1.9389191987033243 7 0.4026347369810458 1.6219784714145404 8 0.27822327199438773 1.2809118566088569 9 0.2088536507999826 1.0817337642141327 >10 1.1559561206809774 12.196988861310258 >50 0.08904723806635881 3.5376936565398025 >100 0.04066055406332023 4.174889937051295 >500 9.002324351545939E-4 0.32388159928435484 >1k 6.001549567697293E-4 0.92165081065348 >5k 1.5003873919243232E-4 0.5676346664077175 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6574 0.5659742220867695 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4648 0.400159443909234 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3137 0.2700731875093088 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1720 0.14807965652407112 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1169 0.10064251074223206 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.609282356050647E-5 0.0 5 0.0 0.0 0.0 8.609282356050647E-5 0.0 6 0.0 0.0 0.0 8.609282356050647E-5 0.0 7 0.0 0.0 0.0 1.7218564712101294E-4 0.0 8 0.0 0.0 0.0 1.7218564712101294E-4 0.0 9 0.0 0.0 0.0 1.7218564712101294E-4 0.0 10-11 0.0 0.0 0.0 1.7218564712101294E-4 0.0 12-13 0.0 0.0 0.0 1.7218564712101294E-4 0.0 14-15 0.0 0.0 0.0 1.7218564712101294E-4 0.0 16-17 0.0 0.0 0.0 1.7218564712101294E-4 0.0 18-19 0.0 0.0 0.0 2.582784706815194E-4 0.0 20-21 0.0 0.0 0.0 2.582784706815194E-4 0.0 22-23 0.0 0.0 0.0 6.026497649235453E-4 0.0 24-25 0.0 0.0 0.0 7.748354120445582E-4 0.0 26-27 0.0 0.0 0.0 9.470210591655711E-4 0.0 28-29 0.0 0.0 0.0 0.0016357636476496229 0.0 30-31 0.0 0.0 0.0 0.004692058884047602 0.0 32-33 0.0 0.0 0.0 0.007791400532225835 0.0 34-35 0.0 0.0 0.0 0.011493391945327613 0.0 36-37 0.0 0.0 0.0 0.01614240441759496 0.0 38-39 0.0 0.0 0.0 0.02384771212626029 0.0 40-41 0.0 0.0 0.0 0.03422189736530132 0.0 42-43 0.0 0.0 0.0 0.04799674913498235 0.0 44-45 0.0 0.0 0.0 0.06039411572769529 0.0 46-47 0.0 0.0 0.0 0.08221864650028368 0.0 48-49 0.0 0.0 0.0 0.11338424862918701 0.0 50-51 0.0 0.0 0.0 0.13344387651878503 0.0 52-53 0.0 0.0 0.0 0.16555649970685393 0.0 54-55 0.0 0.0 0.0 0.21871881825546668 0.0 56-57 0.0 0.0 0.0 0.29865600493139693 0.0 58-59 0.0 0.0 0.0 0.3801859088431966 0.0 60-61 0.0 0.0 0.0 0.4945171785315492 0.0 62-63 0.0 0.0 0.0 0.6106563975146724 0.0 64-65 0.0 0.0 0.0 0.7205538867896588 0.0 66-67 0.0 0.0 0.0 0.8708719567263032 0.0 68-69 0.0 0.0 0.0 1.076375526565232 0.0 70-71 0.0 0.0 0.0 1.2129187447321954 0.0 72-73 0.0 0.0 0.0 1.388462011972068 0.0 74-75 0.0 0.0 0.0 1.5867337846319143 0.0 76-77 0.0 0.0 0.0 1.8041181641221933 0.0 78-79 0.0 0.0 0.0 2.0478899940337674 0.0 80-81 4.3046411780253234E-5 0.0 0.0 2.4048308405156273 0.0 82-83 8.609282356050647E-5 0.0 0.0 2.8118346638979217 0.0 84-85 8.609282356050647E-5 0.0 0.0 3.2715703417110262 0.0 86-87 8.609282356050647E-5 0.0 0.0 3.84688563515411 0.0 88-89 8.609282356050647E-5 0.0 0.0 4.47071423467354 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4110 0.0 41.848618 1 GTATCAA 6870 0.0 37.277466 1 ATCAACG 8595 0.0 29.68539 3 TATCAAC 8650 0.0 29.550293 2 TCAACGC 8745 0.0 29.123133 4 GTACATG 7650 0.0 28.880577 1 CAACGCA 8890 0.0 28.645649 5 AACGCAG 9095 0.0 28.048588 6 TACATGG 7780 0.0 27.480747 2 ACATGGG 7800 0.0 26.74141 3 GTGTAGC 565 0.0 26.069195 1 GTGTACG 150 1.0575568E-6 25.34038 1 ACGCAGA 10330 0.0 24.327347 7 CATGGGG 2530 0.0 24.22715 4 CGCAGAG 10430 0.0 24.139647 8 CATGGGA 5465 0.0 23.47506 4 GAGTACT 5155 0.0 23.0824 12-13 GCAGAGT 10870 0.0 22.768208 9 TAGCGCG 130 2.9351452E-4 21.93012 4 TAAGGTG 565 0.0 21.863543 5 >>END_MODULE