FastQCFastQC Report
Thu 26 May 2016
SRR937192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161537
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96860.8338950890070655No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73670.6342458311702511No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50740.43683498674600985No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18930.16297371500003874No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13690.11786107545433334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43200.048.650291
GTATCAA75200.039.0766681
ATCAACG97600.030.1731823
TATCAAC98300.030.0066382
TCAACGC99250.029.6715624
CAACGCA100650.029.3060365
AACGCAG103300.028.5530036
TAAGGTG4450.027.7518925
GTACATG73100.025.2383671
ACGCAGA117100.024.9468777
CGCAGAG118100.024.6551978
TACATGG74200.024.1973132
GTAAGGT4150.024.035354
GAGTACT69300.023.75023712-13
GCAGAGT124150.023.2624139
ACATGGG74700.022.763643
AGTACTT72150.022.38414612-13
GTACTTT74050.022.19277414-15
TCGCGCA652.944276E-421.92140618-19
AGAGTAC114050.020.82440610-11
Produced by FastQC (version 0.11.5)