##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937192_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1161537 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.289600761749305 33.0 31.0 34.0 31.0 34.0 2 32.4820319972588 34.0 31.0 34.0 31.0 34.0 3 32.527993512044816 34.0 31.0 34.0 31.0 34.0 4 36.15959198889058 37.0 35.0 37.0 35.0 37.0 5 36.09662628052313 37.0 35.0 37.0 35.0 37.0 6 36.14675382704124 37.0 35.0 37.0 35.0 37.0 7 36.09498018573666 37.0 35.0 37.0 35.0 37.0 8 36.068850152857806 37.0 35.0 37.0 35.0 37.0 9 37.78997139135473 39.0 37.0 39.0 35.0 39.0 10-11 37.709981257592304 39.0 37.0 39.0 35.0 39.0 12-13 37.61374583848814 39.0 37.0 39.0 35.0 39.0 14-15 38.81393274600809 40.0 38.0 41.0 35.0 41.0 16-17 38.79248616273094 40.0 38.0 41.0 35.0 41.0 18-19 38.812485525644036 40.0 38.0 41.0 35.0 41.0 20-21 38.74243911300286 40.0 38.0 41.0 34.5 41.0 22-23 38.600634331924 40.0 38.0 41.0 34.0 41.0 24-25 38.530007653652014 40.0 38.0 41.0 34.0 41.0 26-27 38.48033252492172 40.0 38.0 41.0 34.0 41.0 28-29 38.3446657316986 40.0 38.0 41.0 34.0 41.0 30-31 38.37874772822562 40.0 38.0 41.0 34.0 41.0 32-33 38.2807000551855 40.0 38.0 41.0 34.0 41.0 34-35 38.14258779530915 40.0 37.0 41.0 34.0 41.0 36-37 37.96770916466716 40.0 37.0 41.0 33.0 41.0 38-39 37.82867614204282 40.0 37.0 41.0 33.0 41.0 40-41 37.62620303959323 40.0 37.0 41.0 33.0 41.0 42-43 37.382011507166794 40.0 37.0 41.0 33.0 41.0 44-45 37.02663927193021 40.0 36.0 41.0 31.5 41.0 46-47 36.56914846449145 40.0 36.0 41.0 30.5 41.0 48-49 36.16124841481589 39.5 36.0 41.0 30.0 41.0 50-51 35.89393622415817 39.0 35.0 41.0 29.0 41.0 52-53 35.63938212902387 39.0 35.0 41.0 28.0 41.0 54-55 35.410624457077134 39.0 35.0 40.0 28.0 41.0 56-57 35.14802627897346 38.5 35.0 40.0 27.0 41.0 58-59 34.86644248095412 38.0 34.5 40.0 26.0 41.0 60-61 34.546172011739614 38.0 34.0 40.0 26.0 41.0 62-63 34.186632022914466 37.5 34.0 40.0 25.5 41.0 64-65 33.78948109272456 37.0 33.5 39.5 24.5 41.0 66-67 33.3839339599169 36.0 33.0 39.0 23.0 41.0 68-69 32.96082432156703 36.0 33.0 39.0 22.0 40.0 70-71 32.551794303582234 35.0 32.0 38.5 21.5 40.0 72-73 32.105731457542895 35.0 32.0 37.5 20.0 40.0 74-75 31.644182234401487 35.0 31.0 37.0 18.5 39.0 76-77 30.380936638264643 34.0 30.0 35.5 18.0 38.5 78-79 30.977829806540818 34.5 31.0 36.0 18.0 38.5 80-81 30.78004187554938 34.5 31.0 36.0 14.5 37.5 82-83 30.450716593616903 34.0 31.0 35.0 9.5 37.0 84-85 30.106945796819215 34.0 31.0 35.0 7.0 36.5 86-87 29.716947458410708 34.0 30.0 35.0 2.0 36.0 88-89 29.28389065522665 34.0 29.5 35.0 2.0 36.0 90-91 28.81000734371785 34.0 29.0 35.0 2.0 35.0 92-93 28.484787828540977 34.0 29.0 35.0 2.0 35.0 94-95 28.083207422578873 34.0 29.0 35.0 2.0 35.0 96-97 27.6445283275522 33.5 28.0 35.0 2.0 35.0 98-99 26.945756355587466 33.0 26.5 35.0 2.0 35.0 100-101 24.521460358128927 31.0 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.005677508118544949 1101 2 5.0284170469439E-4 1101 3 -0.04185070107421751 1101 4 -0.03557541690835819 1101 5 -0.03847706092726355 1101 6 -0.10838925489144202 1101 7 -0.059526579223572185 1101 8 -0.07811848042050684 1101 9 -0.053435503870503 1101 10-11 -0.04895504325441635 1101 12-13 -0.05344846038425288 1101 14-15 -0.17525795467879846 1101 16-17 -0.12397902150493678 1101 18-19 -0.09595073609604299 1101 20-21 -0.12198095710230206 1101 22-23 -0.10520823392170797 1101 24-25 -0.14722780796094526 1101 26-27 -0.2797555878997571 1101 28-29 -0.22945750643211937 1101 30-31 -0.14822269989882386 1101 32-33 -0.0041717405637626825 1101 34-35 -0.13373741195925248 1101 36-37 -0.23867379519909093 1101 38-39 -0.21777983275786994 1101 40-41 -0.1836416183372762 1101 42-43 -0.32416320598687065 1101 44-45 -0.32161383694754875 1101 46-47 -0.4354641229016991 1101 48-49 -0.32871992458747457 1101 50-51 -0.29082782673586394 1101 52-53 -0.18338308923753033 1101 54-55 -0.22814731235886399 1101 56-57 -0.29513331896301764 1101 58-59 -0.3037255377489174 1101 60-61 -0.3971297600648285 1101 62-63 -0.5400187413880744 1101 64-65 -0.4259618008467001 1101 66-67 -0.3183122715435047 1101 68-69 -0.18424592932422001 1101 70-71 -0.31757172431245806 1101 72-73 -0.21711879190090855 1101 74-75 -0.28449867677586127 1101 76-77 -0.13776567244594773 1101 78-79 -0.25759925945862605 1101 80-81 -0.30404907809588266 1101 82-83 -0.3248541522868429 1101 84-85 -0.21045226459645505 1101 86-87 -0.22414281908584854 1101 88-89 -0.24323142036713463 1101 90-91 -0.2660913956268267 1101 92-93 -0.1805132671296228 1101 94-95 -0.09679873725902155 1101 96-97 -0.022717323811694 1101 98-99 0.20051430245126411 1101 100-101 0.7686170111177795 1102 1 0.009034976091193414 1102 2 0.019607629923036995 1102 3 0.03473517331708109 1102 4 0.0020484664417566023 1102 5 0.031204256606287117 1102 6 -0.009172215400518269 1102 7 -0.10979567878942476 1102 8 -0.09743492219705274 1102 9 -0.035543880739957956 1102 10-11 -0.028493228591194963 1102 12-13 -0.10955813397663405 1102 14-15 0.01165105596891891 1102 16-17 -0.07770434090670619 1102 18-19 -0.1145855115245169 1102 20-21 0.07014785596267359 1102 22-23 -0.06494479027495714 1102 24-25 -0.03795052406336907 1102 26-27 -0.04982178383104241 1102 28-29 -0.07172003173102581 1102 30-31 -0.04401406545841269 1102 32-33 0.06934436895217289 1102 34-35 -0.027834169560740918 1102 36-37 -0.07863966261410127 1102 38-39 0.0726811739169051 1102 40-41 0.1463822468823679 1102 42-43 0.26822889306857434 1102 44-45 0.3004168547890984 1102 46-47 0.4225758547899403 1102 48-49 0.5162239399227175 1102 50-51 0.6316360256347906 1102 52-53 0.512289245829372 1102 54-55 0.5221710547950309 1102 56-57 0.6495529745969222 1102 58-59 0.6029963348923886 1102 60-61 0.678823518496074 1102 62-63 0.5937624671655612 1102 64-65 0.45697328615001 1102 66-67 0.4737111088830659 1102 68-69 0.5406338084933893 1102 70-71 0.47514258476874005 1102 72-73 0.6076649726551935 1102 74-75 0.5197068045389166 1102 76-77 0.6465956126892323 1102 78-79 0.5854631743996386 1102 80-81 0.36726752921976313 1102 82-83 0.35007751356855366 1102 84-85 0.3564724328512838 1102 86-87 0.509030033290724 1102 88-89 0.5711385555705739 1102 90-91 0.5960632139472466 1102 92-93 0.4404936441276277 1102 94-95 0.6040928090140838 1102 96-97 0.6896246011320919 1102 98-99 0.8726477663765486 1102 100-101 1.5617871439234925 1103 1 0.030826165617099832 1103 2 0.08192998435625043 1103 3 -0.10386007454744117 1103 4 -0.04404799370222179 1103 5 -0.08740516560267508 1103 6 -0.0032040703745934707 1103 7 0.007053140636884336 1103 8 -0.06981733108958821 1103 9 -0.07442634558385919 1103 10-11 -0.07133331960540801 1103 12-13 -0.08358924293258241 1103 14-15 -0.1759874236461414 1103 16-17 -0.2048594007746587 1103 18-19 -0.09016460809656479 1103 20-21 -0.06016286058773801 1103 22-23 -5.447753545979594E-4 1103 24-25 0.011633570830134943 1103 26-27 0.033321936561364396 1103 28-29 -0.08461122498702167 1103 30-31 -0.07115793505444401 1103 32-33 -0.04531861735839726 1103 34-35 -0.1142379891735601 1103 36-37 -0.03821667033538745 1103 38-39 0.08637060657011375 1103 40-41 0.05007130652651881 1103 42-43 -0.12881687514359186 1103 44-45 -0.3089009116326267 1103 46-47 -0.10438897045531093 1103 48-49 -0.15098483325046885 1103 50-51 0.009110008252562807 1103 52-53 0.12423672615313563 1103 54-55 -0.04955485814465277 1103 56-57 -0.056665086501965334 1103 58-59 -0.08413485239546503 1103 60-61 -0.07827670903945005 1103 62-63 0.013826624724593728 1103 64-65 -0.012689886665093297 1103 66-67 0.0081995269516284 1103 68-69 -0.002829207657910615 1103 70-71 0.15930834999684151 1103 72-73 0.24302996202444405 1103 74-75 0.2844611780701918 1103 76-77 0.3407319102012636 1103 78-79 0.12080205313439052 1103 80-81 0.055782580135499416 1103 82-83 0.02357148947216814 1103 84-85 -0.025466468264013997 1103 86-87 0.02526338776123893 1103 88-89 -0.19289964055759157 1103 90-91 0.2006512254359265 1103 92-93 0.11373024377699892 1103 94-95 0.28558036916179574 1103 96-97 0.4304243325655648 1103 98-99 0.7272637535450386 1103 100-101 1.4273509471239088 1104 1 0.08353063345012401 1104 2 0.08811462292261041 1104 3 0.04125645575957293 1104 4 0.05375401139918523 1104 5 0.05355426944429098 1104 6 0.051645979342481496 1104 7 0.056005147511299924 1104 8 0.010640289417835902 1104 9 0.05435568573862781 1104 10-11 0.07458640736115996 1104 12-13 0.07744937997011192 1104 14-15 0.053103596726934654 1104 16-17 -0.039459179561625035 1104 18-19 0.06547144253664072 1104 20-21 0.03008054661766124 1104 22-23 -0.15453921539755555 1104 24-25 -0.04379574227763072 1104 26-27 -0.0846721945411204 1104 28-29 -0.13935149396925084 1104 30-31 -0.014557951278675318 1104 32-33 0.11029654578986481 1104 34-35 0.18117662497387244 1104 36-37 0.16731763881590922 1104 38-39 0.2193410643800675 1104 40-41 0.1734099621205516 1104 42-43 0.2687727576689909 1104 44-45 0.25632074587188214 1104 46-47 0.13575104369960656 1104 48-49 0.30537805328336987 1104 50-51 0.2237017137837256 1104 52-53 0.22534624020823202 1104 54-55 0.1502939261794367 1104 56-57 0.20756378398542807 1104 58-59 0.06751484602325775 1104 60-61 0.24823086947441908 1104 62-63 0.20541173709683846 1104 64-65 0.2935643606592393 1104 66-67 0.45430301521019345 1104 68-69 0.3781840199491242 1104 70-71 0.44618046855614324 1104 72-73 0.4510710122471835 1104 74-75 0.2954955991881185 1104 76-77 0.1576846787402424 1104 78-79 0.15106899033446908 1104 80-81 0.17230851534835168 1104 82-83 0.2715749130766767 1104 84-85 0.4931532673004284 1104 86-87 0.6274942683047406 1104 88-89 0.39971765359988964 1104 90-91 0.34552730678830557 1104 92-93 0.42114836119283794 1104 94-95 0.442903691398854 1104 96-97 0.35817233623204814 1104 98-99 0.6696167238668345 1104 100-101 1.1894747963084988 1105 1 -0.08144653757432962 1105 2 -0.08163425351283848 1105 3 0.011121047984673282 1105 4 -0.007217189193220008 1105 5 -0.0853244957899264 1105 6 -0.13368150987623295 1105 7 -0.20085490405327988 1105 8 -0.16367438212623142 1105 9 -0.13304395715683626 1105 10-11 -0.07317308553880508 1105 12-13 -0.026074441338415966 1105 14-15 -0.06077664274771877 1105 16-17 -0.14550008514159884 1105 18-19 -0.13768589710225143 1105 20-21 -0.15189775836352482 1105 22-23 -0.2012124589488451 1105 24-25 -0.36892883477002414 1105 26-27 -0.2895884721855424 1105 28-29 -0.23353080607416388 1105 30-31 -0.20467116327638735 1105 32-33 -0.3152366700553699 1105 34-35 -0.2131732639989039 1105 36-37 -0.154987328842644 1105 38-39 -0.3067810259285153 1105 40-41 -0.28478033766744915 1105 42-43 -0.05957200384723649 1105 44-45 0.04229221231091884 1105 46-47 0.1165876098891303 1105 48-49 0.30572993755597366 1105 50-51 0.21424965753250547 1105 52-53 0.3120545368466736 1105 54-55 0.3990898826037892 1105 56-57 0.38212883472346704 1105 58-59 0.45455371086779905 1105 60-61 0.2400725196183089 1105 62-63 0.3603263713256979 1105 64-65 0.503086068728706 1105 66-67 0.36050845568906453 1105 68-69 0.5280944538011667 1105 70-71 0.5353782980875508 1105 72-73 0.556370689074349 1105 74-75 0.5193679502680872 1105 76-77 0.47731716268552304 1105 78-79 0.5161375642654491 1105 80-81 0.2858025521666612 1105 82-83 0.28081260026895905 1105 84-85 0.36523439209270236 1105 86-87 0.3502602140275535 1105 88-89 0.4011500836024702 1105 90-91 0.4401114308139782 1105 92-93 0.6364391829102836 1105 94-95 0.7942081766666007 1105 96-97 0.5096084880875118 1105 98-99 0.6615939557943413 1105 100-101 1.0105090170540088 1106 1 -0.07315955551734277 1106 2 0.038352140915030475 1106 3 -0.025879316033368127 1106 4 -0.04249350859941359 1106 5 -0.08922521968702313 1106 6 -0.06195987436335315 1106 7 -0.054247966060515296 1106 8 -0.01936882347528268 1106 9 -0.15721454258910939 1106 10-11 -0.15824930115210378 1106 12-13 -0.2169968609227979 1106 14-15 -0.18031385165268432 1106 16-17 -0.14902405567629984 1106 18-19 -0.11756594095422201 1106 20-21 -0.1312617562367251 1106 22-23 -0.11961006852835254 1106 24-25 -0.16295803687008714 1106 26-27 -0.16078419184283632 1106 28-29 -0.2592566930630156 1106 30-31 -0.1819948559687532 1106 32-33 -0.1657645492735469 1106 34-35 -0.18692443273028658 1106 36-37 -0.18224278141894246 1106 38-39 -0.0915720763911807 1106 40-41 0.10835463236739429 1106 42-43 -0.051907991434539724 1106 44-45 -0.0337565151648036 1106 46-47 -0.09877495950512838 1106 48-49 -0.23444895130814558 1106 50-51 -0.18682870610559377 1106 52-53 -0.14241959505520896 1106 54-55 -0.21306296039831096 1106 56-57 -0.2072296338961621 1106 58-59 -0.07645468277260647 1106 60-61 -0.10492133028102302 1106 62-63 -0.07298456906130468 1106 64-65 0.02311953079495055 1106 66-67 0.043879092911659257 1106 68-69 0.05503603368190113 1106 70-71 0.186352391252818 1106 72-73 0.06776323317836841 1106 74-75 0.12193082828171242 1106 76-77 0.17029368405274425 1106 78-79 0.16169940965648166 1106 80-81 0.025921001301238533 1106 82-83 -0.0751028520314101 1106 84-85 0.05556833353780277 1106 86-87 0.00448181504892986 1106 88-89 0.32062011366845056 1106 90-91 0.37285644519958794 1106 92-93 0.4706576213259659 1106 94-95 0.4939317670518548 1106 96-97 0.4675933245606352 1106 98-99 0.39541611334941607 1106 100-101 0.769682696108962 1107 1 -0.14141104645338487 1107 2 -0.09729345504632647 1107 3 -0.13753150314891371 1107 4 -0.07077998713238998 1107 5 -0.011406284408494116 1107 6 -0.14849110084943362 1107 7 -0.14849399116138073 1107 8 -0.07441400369378215 1107 9 -0.1863863167304416 1107 10-11 -0.06207583233393166 1107 12-13 -0.12328056188267311 1107 14-15 -0.06865803627645306 1107 16-17 -0.09695404922358364 1107 18-19 -0.14768190016587823 1107 20-21 -0.22357475568076524 1107 22-23 -0.06032149788445196 1107 24-25 -0.06089059810954467 1107 26-27 -0.19221023226054967 1107 28-29 -0.2570802948429858 1107 30-31 -0.22294145243694174 1107 32-33 -0.19094607394683294 1107 34-35 -0.10562376267426288 1107 36-37 -0.13114905618880357 1107 38-39 -0.3228273652788829 1107 40-41 -0.2623347148359443 1107 42-43 -0.14306245933057227 1107 44-45 -0.09037468594089404 1107 46-47 -0.2324328268023308 1107 48-49 -0.1388141396539524 1107 50-51 -0.11291684939621405 1107 52-53 -0.17356067746026582 1107 54-55 -0.058163301343178375 1107 56-57 0.028358635546290145 1107 58-59 -0.006422274031734787 1107 60-61 0.12886508908339067 1107 62-63 -0.06520139168735994 1107 64-65 -0.21657174329391182 1107 66-67 -0.17701304615454916 1107 68-69 0.13578520811304884 1107 70-71 0.16568470197483265 1107 72-73 0.04247034881159806 1107 74-75 0.06459454098598272 1107 76-77 0.14147301390741518 1107 78-79 -0.02109096841944691 1107 80-81 -0.0291691135304184 1107 82-83 -0.10358804761140661 1107 84-85 0.0493670778246873 1107 86-87 0.007748924251760769 1107 88-89 -0.09368790445276076 1107 90-91 -0.045633003818323914 1107 92-93 0.007416088570028734 1107 94-95 -0.11991523868414333 1107 96-97 0.03387522813597599 1107 98-99 0.3267281147426644 1107 100-101 0.8648410892248641 1108 1 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-0.2977423000059005 2316 66-67 -0.4385764141387085 2316 68-69 -0.4198156030072724 2316 70-71 -0.345166088582765 2316 72-73 -0.09972245992977946 2316 74-75 -0.055511531027836014 2316 76-77 -0.09760818037551644 2316 78-79 -0.49701122934457587 2316 80-81 -0.4766748552752773 2316 82-83 -0.42856289781988366 2316 84-85 -0.28362011634435547 2316 86-87 -0.4104541468699523 2316 88-89 -0.1560380338397387 2316 90-91 -0.2990155716911289 2316 92-93 0.01241994803489277 2316 94-95 0.11754940925040813 2316 96-97 0.15317460872349997 2316 98-99 0.3487460454368012 2316 100-101 -0.16856463543711087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 10.0 5 20.0 6 39.0 7 108.0 8 269.0 9 506.0 10 950.0 11 1549.0 12 2507.0 13 3826.0 14 4901.0 15 7282.0 16 10341.0 17 15499.0 18 13543.0 19 7407.0 20 5826.0 21 5863.0 22 5889.0 23 6168.0 24 7009.0 25 8324.0 26 10006.0 27 12326.0 28 15280.0 29 19322.0 30 23923.0 31 30620.0 32 39271.0 33 52490.0 34 73053.0 35 108623.0 36 167803.0 37 261276.0 38 219096.0 39 20606.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10432685059551 19.382050785035858 15.16125002692167 21.352372337446965 2 15.720807860619162 20.77368176820885 39.90669259782512 23.598817773346866 3 19.476779474093377 25.733919797647424 30.14359422041657 24.645706507842625 4 12.696883525879935 17.048789664039973 37.201656081554006 33.05267072852608 5 13.16940205922843 38.41729207759257 33.377615038445875 15.035690824733125 6 31.305416874365605 36.69138391631089 17.176207042909525 14.826992166413985 7 28.572744561731568 33.95277119885118 19.865919036586867 17.608565202830388 8 27.72714084872027 34.13855951209475 19.08135513547997 19.052944503705007 9 27.529816097119593 16.34360334625587 19.957177429561003 36.16940312706354 10-11 25.05670295914331 27.50987808809976 26.25132852159921 21.18209043115772 12-13 26.41778207121714 24.777737024543207 27.700734074812605 21.10374682942705 14-15 23.194095409788925 25.450975733015824 26.83711323875176 24.517815618443496 16-17 21.578047018734658 29.228470552380166 27.33929267858019 21.854189750304982 18-19 21.556782091315213 26.537553259172974 31.137751100481516 20.767913549030293 20-21 22.381077830495283 25.42174721941703 31.5430330673926 20.654141882695086 22-23 21.968384693928506 25.728438278609346 31.386426132518775 20.916750894943377 24-25 21.667373351039902 25.772872359888165 31.61647055278502 20.943283736286915 26-27 21.512675291199592 26.15008719091992 31.317187695727583 21.02004982215291 28-29 21.214467992781675 26.239272381634592 31.534247448101056 21.012012177482678 30-31 21.37341028916982 25.93269061894119 31.60278838637248 21.09111070551651 32-33 21.133334538632866 26.08281096512638 31.406059385107838 21.377795111132922 34-35 21.638613320109474 26.01281749957169 31.301241372422915 21.047327807895915 36-37 21.698146507601564 25.829095414093565 31.191688254442173 21.28106982386269 38-39 21.52844888705032 26.068493250930562 31.27136997263524 21.131687889383876 40-41 21.535311202015606 26.079077153408438 31.237201758188398 21.148409886387558 42-43 21.54805617945118 26.09994700295466 30.899214774078033 21.452782043516127 44-45 21.6323693052067 26.333055682901684 30.59074972073532 21.443825291156298 46-47 21.752944589797828 26.761954203783432 29.932580709869764 21.55252049654897 48-49 21.97093161905303 26.94167297296599 29.478527158411655 21.608868249569323 50-51 22.081819175798962 27.02419294434874 29.269536829218527 21.624451050633773 52-53 22.282372408283162 27.244160108545834 28.829645547236122 21.643821935934888 54-55 21.812047313172116 27.211229603533937 29.016423927950637 21.960299155343307 56-57 21.930338852744253 27.4940445289302 28.819400501232415 21.75621611709313 58-59 22.14905767099972 26.933666340374867 29.042036542959888 21.875239445665528 60-61 21.951216362457675 26.918557049839997 29.505818583480337 21.624408004221994 62-63 21.981994546880557 27.072189693483722 29.442540358163367 21.50327540147236 64-65 21.776490977041625 26.997977679574564 29.325238886062177 21.900292457321633 66-67 21.677570322770602 27.387031149244496 29.0611921962021 21.8742063317828 68-69 21.83550760759235 27.094530781197673 29.299970642347166 21.76999096886281 70-71 21.78953403981104 27.407306009192993 28.99481462923695 21.808345321759013 72-73 21.774726074158636 27.355392036586007 28.791420333575253 22.078461555680104 74-75 21.701934591838228 27.38724638130339 28.696287763540894 22.214531263317483 76-77 21.76999096886281 27.26882570249592 28.932784749861607 22.02839857877967 78-79 21.728924692024446 27.30515687403845 28.790344173280747 22.175574260656354 80-81 21.692310871122373 27.58020414347898 28.602944721472255 22.124540263926388 82-83 21.69925232140236 27.478017112795765 28.635113128950344 22.18761743685153 84-85 21.63548376543433 27.273071644910956 28.56003354185069 22.531411047804024 86-87 21.65824248388127 27.135812290094936 28.586002856559883 22.619942369463907 88-89 21.512659519240454 27.046706217709808 28.64433074452213 22.796303518527605 90-91 21.338634201361234 27.10274700911365 28.80269836281529 22.75592042670983 92-93 21.394963409384417 26.837752905725353 29.039733103745156 22.72755058114507 94-95 21.0781059923188 26.83741456363422 29.018317970068967 23.066161473978013 96-97 21.180642545179364 26.782056878084816 28.837049530062327 23.200251046673504 98-99 21.105803005675245 26.76727195713263 28.70040188164637 23.426523155545762 100-101 20.753033923894996 26.791649966398555 28.954756229182067 23.50055988052438 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 358.0 1 268.5 2 314.5 3 472.0 4 370.5 5 284.5 6 617.0 7 1507.5 8 2542.0 9 2996.0 10 2613.5 11 2130.0 12 2113.5 13 2264.0 14 2412.5 15 2534.5 16 2625.0 17 2770.5 18 2888.5 19 2961.5 20 3144.5 21 3428.5 22 3922.5 23 4399.5 24 4998.5 25 5960.0 26 7101.5 27 8690.0 28 10170.5 29 12240.0 30 15095.0 31 17057.5 32 19068.5 33 21082.5 34 23221.0 35 25262.5 36 27759.5 37 30603.5 38 32037.5 39 32504.5 40 33691.5 41 36123.0 42 38231.0 43 40262.5 44 42217.0 45 43556.0 46 43337.5 47 43278.5 48 45638.0 49 45437.5 50 43165.0 51 41142.0 52 38188.5 53 36673.0 54 35594.5 55 32976.0 56 29493.5 57 26351.5 58 24233.0 59 21759.0 60 18245.5 61 14727.0 62 11637.0 63 8853.0 64 6419.0 65 4753.5 66 3480.0 67 2663.0 68 1968.5 69 1261.0 70 853.0 71 610.5 72 466.5 73 411.0 74 346.0 75 274.0 76 205.5 77 135.5 78 89.0 79 57.0 80 41.5 81 26.0 82 22.0 83 15.0 84 8.0 85 3.0 86 1.0 87 1.0 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06560273155310593 2 0.0 3 0.0 4 0.0 5 0.0029271560010572196 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.291392353407597E-4 12-13 0.006456961767037985 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.47021059165571E-4 24-25 8.178818238248114E-4 26-27 4.735105295827855E-4 28-29 0.004218548354464817 30-31 4.304641178025323E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.59603353143292E-4 40-41 4.3046411780253234E-5 42-43 0.01304306276941673 44-45 3.8741770602227914E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.609282356050647E-5 82-83 7.317890002643049E-4 84-85 2.582784706815194E-4 86-87 0.0 88-89 0.0 90-91 0.007920539767566596 92-93 0.0031854344717387392 94-95 0.0 96-97 0.0 98-99 8.609282356050647E-5 100-101 0.011062927827525082 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1161537.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.78869161252427 #Duplication Level Percentage of deduplicated Percentage of total 1 77.22889035990404 42.31269857506231 2 12.758535346699084 13.98046917075573 3 4.151866306653583 6.824259680750197 4 1.8702984783397718 4.098848262125246 5 1.0064478710559523 2.757098101568308 6 0.619414758006708 2.036215449400552 7 0.41323431735696076 1.5848397304167754 8 0.30159431656013447 1.3219166401682583 9 0.2291161383963456 1.129767610504483 >10 1.2705924644648343 12.784606650415672 >50 0.10192197624516976 3.8658328605817203 >100 0.04493437947261002 4.356776629705292 >500 0.0023649651337302193 0.7535121945365633 >1k 3.1532868449736257E-4 0.28177883510011115 >5k 4.7299302674604386E-4 1.9113796089086939 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9686 0.8338950890070655 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7367 0.6342458311702511 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5074 0.43683498674600985 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1893 0.16297371500003874 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1369 0.11786107545433334 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.1523205890126618E-4 0.0 24-25 0.0 0.0 0.0 3.4437129424202587E-4 0.0 26-27 0.0 0.0 0.0 5.165569413630388E-4 0.0 28-29 0.0 0.0 0.0 0.0012052995298470906 0.0 30-31 0.0 0.0 0.0 0.00426159476624507 0.0 32-33 0.0 0.0 0.0 0.0073178900026430495 0.0 34-35 0.0 0.0 0.0 0.010933788592184322 0.0 36-37 0.0 0.0 0.0 0.015754986711572685 0.0 38-39 0.0 0.0 0.0 0.023288108773117 0.0 40-41 0.0 0.0 0.0 0.03370534042393828 0.0 42-43 0.0 0.0 0.0 0.047652377840740334 0.0 44-45 0.0 0.0 0.0 0.060092790845233515 0.0 46-47 0.0 0.0 0.0 0.08243387855918494 0.0 48-49 0.0 0.0 0.0 0.11415908404123157 0.0 50-51 0.0 0.0 0.0 0.13439089757795059 8.609282356050647E-5 52-53 0.0 0.0 0.0 0.16646047435423927 8.609282356050647E-5 54-55 0.0 0.0 0.0 0.219967164197094 8.609282356050647E-5 56-57 0.0 0.0 0.0 0.299689118814123 8.609282356050647E-5 58-59 0.0 0.0 0.0 0.3807455121963399 8.609282356050647E-5 60-61 0.0 0.0 0.0 0.4950767818846924 8.609282356050647E-5 62-63 0.0 0.0 0.0 0.6121630219269812 8.609282356050647E-5 64-65 0.0 0.0 0.0 0.7226631609668912 8.609282356050647E-5 66-67 0.0 0.0 0.0 0.874401762492284 8.609282356050647E-5 68-69 0.0 0.0 0.0 1.0813258639199612 8.609282356050647E-5 70-71 0.0 0.0 0.0 1.219117428028552 8.609282356050647E-5 72-73 0.0 8.609282356050647E-5 0.0 1.3962103660925136 8.609282356050647E-5 74-75 0.0 8.609282356050647E-5 0.0 1.5964622736942515 8.609282356050647E-5 76-77 0.0 8.609282356050647E-5 0.0 1.8160420201853233 8.609282356050647E-5 78-79 0.0 8.609282356050647E-5 0.0 2.0605025926853813 8.609282356050647E-5 80-81 0.0 8.609282356050647E-5 0.0 2.419251388462012 8.609282356050647E-5 82-83 0.0 8.609282356050647E-5 0.0 2.8273313721388122 8.609282356050647E-5 84-85 0.0 8.609282356050647E-5 0.0 3.292835269130471 8.609282356050647E-5 86-87 0.0 8.609282356050647E-5 0.0 3.874478385105253 8.609282356050647E-5 88-89 0.0 8.609282356050647E-5 0.0 4.506830174157173 8.609282356050647E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4320 0.0 48.65029 1 GTATCAA 7520 0.0 39.076668 1 ATCAACG 9760 0.0 30.173182 3 TATCAAC 9830 0.0 30.006638 2 TCAACGC 9925 0.0 29.671562 4 CAACGCA 10065 0.0 29.306036 5 AACGCAG 10330 0.0 28.553003 6 TAAGGTG 445 0.0 27.751892 5 GTACATG 7310 0.0 25.238367 1 ACGCAGA 11710 0.0 24.946877 7 CGCAGAG 11810 0.0 24.655197 8 TACATGG 7420 0.0 24.197313 2 GTAAGGT 415 0.0 24.03535 4 GAGTACT 6930 0.0 23.750237 12-13 GCAGAGT 12415 0.0 23.262413 9 ACATGGG 7470 0.0 22.76364 3 AGTACTT 7215 0.0 22.384146 12-13 GTACTTT 7405 0.0 22.192774 14-15 TCGCGCA 65 2.944276E-4 21.921406 18-19 AGAGTAC 11405 0.0 20.824406 10-11 >>END_MODULE