FastQCFastQC Report
Thu 26 May 2016
SRR937191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100566
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100240.9108040771748356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71420.6489388187532596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57940.5264563869863325No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18160.1650060060005488No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16340.14846906046525152No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16240.14756043708419123No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15030.13656609417336169No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12850.11675810446624736No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12010.10912566806534091No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT11810.10730842130322034No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAG459.6207974E-442.225877
CATTCCG2700.042.2258649
TAGGACG608.272962E-539.5975724
GGTATCA43000.038.367911
GTATCAA71150.036.3522341
AGATCGA600.003949207331.6693976
GTACATG53750.029.2790281
ATCAACG88100.029.1777213
TCAACGC88550.028.9771044
CAACGCA89350.028.7150385
TATCAAC91400.028.0761092
AACGCAG92050.027.8676956
ACATGGG53200.027.1513843
TACATGG57550.026.8365942
CCCTATA5950.026.3564152
GACAATG6100.025.6989387
GGACAAT6550.025.383876
ATGGGTA4700.025.2727435
TTAGGCA4650.024.5249464
ACGCAGA103450.024.4752887
Produced by FastQC (version 0.11.5)