##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937191_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1100566 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31018221533284 33.0 31.0 34.0 31.0 34.0 2 32.468639772626084 34.0 31.0 34.0 31.0 34.0 3 32.49525698595087 34.0 31.0 34.0 31.0 34.0 4 36.084819083998596 37.0 35.0 37.0 35.0 37.0 5 36.10937463087175 37.0 37.0 37.0 35.0 37.0 6 36.1788252590031 37.0 37.0 37.0 35.0 37.0 7 36.145072626266845 37.0 37.0 37.0 35.0 37.0 8 36.14020513081451 37.0 37.0 37.0 35.0 37.0 9 37.85128106810496 39.0 38.0 39.0 35.0 39.0 10-11 37.78013585736794 39.0 38.0 39.0 35.0 39.0 12-13 37.69358630014011 39.0 38.0 39.0 35.0 39.0 14-15 38.94001313869409 40.0 38.0 41.0 36.0 41.0 16-17 38.87938206341101 40.0 38.0 41.0 36.0 41.0 18-19 38.87340241294025 40.0 38.0 41.0 36.0 41.0 20-21 38.79394011808469 40.0 38.0 41.0 36.0 41.0 22-23 38.852418664578046 40.0 38.0 41.0 36.0 41.0 24-25 38.8470668728636 40.0 38.0 41.0 35.5 41.0 26-27 38.76526350986674 40.0 38.0 41.0 35.0 41.0 28-29 38.65255786568002 40.0 38.0 41.0 35.0 41.0 30-31 38.39195377651136 40.0 38.0 41.0 35.0 41.0 32-33 38.14083526112927 40.0 38.0 41.0 34.5 41.0 34-35 38.110457709941976 40.0 38.0 41.0 35.0 41.0 36-37 37.9843794011445 40.0 38.0 41.0 34.0 41.0 38-39 37.85785404964355 40.0 38.0 41.0 34.0 41.0 40-41 37.66752607294792 40.0 37.5 41.0 33.5 41.0 42-43 37.393291724439976 40.0 37.0 41.0 33.0 41.0 44-45 37.093505977833225 40.0 37.0 41.0 33.0 41.0 46-47 36.63844649026046 40.0 37.0 41.0 31.5 41.0 48-49 36.25828346505344 40.0 36.5 41.0 30.5 41.0 50-51 35.33049358239306 38.5 35.0 40.0 29.0 40.5 52-53 35.444063781726854 38.5 35.5 40.0 29.5 40.5 54-55 35.80182742334399 39.0 36.0 40.0 29.5 41.0 56-57 35.607917198968536 39.0 36.0 40.0 28.0 41.0 58-59 35.41654248813792 39.0 35.0 40.0 28.0 41.0 60-61 35.1452861527614 39.0 35.0 40.0 27.0 41.0 62-63 34.82933281602375 38.0 35.0 40.0 26.0 41.0 64-65 34.467075123163895 38.0 34.5 40.0 26.0 41.0 66-67 34.05879156724812 37.5 34.0 40.0 24.5 41.0 68-69 33.586197919979355 37.0 34.0 39.0 22.5 41.0 70-71 33.1469362128214 36.5 33.0 39.0 21.5 40.0 72-73 32.66960364030871 36.0 33.0 39.0 19.5 40.0 74-75 32.11426302466185 35.5 32.0 38.0 17.5 40.0 76-77 31.600868553089956 35.0 31.5 37.0 11.5 39.0 78-79 31.04780858212956 35.0 31.0 37.0 7.5 39.0 80-81 30.464354704761007 34.5 30.5 36.0 2.0 38.5 82-83 29.90336108874888 34.0 30.0 36.0 2.0 37.0 84-85 29.313231555399675 34.0 29.0 35.0 2.0 37.0 86-87 28.757817795570645 34.0 29.0 35.0 2.0 36.0 88-89 28.195200923888255 34.0 28.5 35.0 2.0 36.0 90-91 27.63938146371958 33.5 26.5 35.0 2.0 35.5 92-93 27.019318241704724 33.0 26.0 35.0 2.0 35.0 94-95 26.288910887670525 32.5 25.0 35.0 2.0 35.0 96-97 25.469667879981756 32.0 22.0 34.0 2.0 35.0 98-99 24.355471639138408 32.0 9.5 34.0 2.0 35.0 100-101 21.015144025892134 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2558577156736632 1101 2 0.2240001173178925 1101 3 0.15475748852122706 1101 4 0.10682199361901468 1101 5 -0.01954537178396265 1101 6 0.08053925403763884 1101 7 0.17012064008690686 1101 8 0.1452664626823008 1101 9 0.25505142839077166 1101 10-11 0.2404780316332804 1101 12-13 0.3223065754880352 1101 14-15 0.44864472166703706 1101 16-17 0.3341637188378641 1101 18-19 0.33247096194866543 1101 20-21 0.43529917730271706 1101 22-23 0.3842938118456303 1101 24-25 0.44361677804140953 1101 26-27 0.38032080238031796 1101 28-29 0.3372610490365915 1101 30-31 0.44883708566818115 1101 32-33 0.29060681559635526 1101 34-35 0.3865954576058286 1101 36-37 0.4939152461757885 1101 38-39 0.4479333380219188 1101 40-41 0.4222105139674568 1101 42-43 0.4470347241107788 1101 44-45 0.5441232440425026 1101 46-47 0.6815239238403024 1101 48-49 0.6978784945032643 1101 50-51 0.6921379242539629 1101 52-53 0.6372076348972584 1101 54-55 0.7459748791778438 1101 56-57 0.7788501854447247 1101 58-59 0.724971604163855 1101 60-61 0.8120704484853078 1101 62-63 0.7442570236983812 1101 64-65 0.6162808651640219 1101 66-67 0.9917539511840445 1101 68-69 0.7890745616951023 1101 70-71 0.827485066539424 1101 72-73 0.7849892869444517 1101 74-75 0.7491336979260872 1101 76-77 0.9537302125449365 1101 78-79 0.9828756247205668 1101 80-81 1.143340137796372 1101 82-83 0.9387581978164974 1101 84-85 1.1095902157857687 1101 86-87 1.1929308678085206 1101 88-89 1.3771232953662143 1101 90-91 1.2771748035743116 1101 92-93 1.3616756186070837 1101 94-95 1.4932571371184125 1101 96-97 1.7657426139911507 1101 98-99 1.9876665955091433 1101 100-101 2.2858335155701113 1102 1 0.14381990925188148 1102 2 0.09772996484236529 1102 3 0.07010621819706842 1102 4 0.018903063625906213 1102 5 0.03309084555947095 1102 6 0.017148945538522753 1102 7 -0.0479484305700808 1102 8 0.016795361154798627 1102 9 0.06165175756026997 1102 10-11 0.09368857879173476 1102 12-13 0.14385573230593707 1102 14-15 0.2745654462977498 1102 16-17 0.3306442605254176 1102 18-19 0.30777008729174327 1102 20-21 0.3703826256622449 1102 22-23 0.3408865870558202 1102 24-25 0.3160412785072353 1102 26-27 0.34089714658448145 1102 28-29 0.2529748867454984 1102 30-31 0.37702811843176676 1102 32-33 0.3776158368550284 1102 34-35 0.40130278746433845 1102 36-37 0.5398713599613103 1102 38-39 0.46424760104162743 1102 40-41 0.4904462295448653 1102 42-43 0.7076245738785474 1102 44-45 0.6101076508392396 1102 46-47 0.6438302391791879 1102 48-49 0.8917136625330713 1102 50-51 0.6316890817265417 1102 52-53 0.7359373107408445 1102 54-55 0.8876276396921483 1102 56-57 0.9144660734813073 1102 58-59 1.0432221987520194 1102 60-61 0.9263956166753573 1102 62-63 0.9889263319617285 1102 64-65 0.9204570601202633 1102 66-67 0.9423859657792164 1102 68-69 1.0445225687088424 1102 70-71 1.0580319045207318 1102 72-73 1.2427975628791401 1102 74-75 1.075687352783394 1102 76-77 1.2163161388320312 1102 78-79 1.3060288533514388 1102 80-81 1.4931210729765354 1102 82-83 1.6572868009978592 1102 84-85 1.8493282765241794 1102 86-87 1.6439846451315354 1102 88-89 1.5255568788958342 1102 90-91 1.6157981974995543 1102 92-93 1.7978103263031961 1102 94-95 2.003326793779518 1102 96-97 1.9306541296587483 1102 98-99 2.205523926397742 1102 100-101 2.0306899560071443 1103 1 0.2372866944226999 1103 2 0.1969569945626688 1103 3 0.1288836054497935 1103 4 0.13041143298180913 1103 5 0.061467877299733686 1103 6 0.10204774568395436 1103 7 0.059052067686018006 1103 8 0.08278399552908411 1103 9 0.15201117527394814 1103 10-11 0.15727346412550247 1103 12-13 0.10510622046225393 1103 14-15 0.12673993019869556 1103 16-17 0.09156717895954358 1103 18-19 0.011518117833496433 1103 20-21 0.09846272369750153 1103 22-23 0.0923678889855637 1103 24-25 0.1458245371021505 1103 26-27 0.10100703225490548 1103 28-29 -0.0028324901381111545 1103 30-31 0.10365384729963978 1103 32-33 0.22775184730493692 1103 34-35 0.3403219635911441 1103 36-37 0.3125554790579912 1103 38-39 0.30481069777064107 1103 40-41 0.41059227244875274 1103 42-43 0.3597458655738279 1103 44-45 0.5621521841617962 1103 46-47 0.4483152174309808 1103 48-49 0.4808678391312 1103 50-51 0.42278511049073586 1103 52-53 0.417979218119072 1103 54-55 0.5903774747289887 1103 56-57 0.5343589484190261 1103 58-59 0.5164341999909254 1103 60-61 0.43693305994822396 1103 62-63 0.5414031361201452 1103 64-65 0.46574786662183243 1103 66-67 0.7340021335164622 1103 68-69 0.6625150006026175 1103 70-71 0.7877953002034062 1103 72-73 0.7685486502894179 1103 74-75 0.8560018601158355 1103 76-77 0.8590614626311641 1103 78-79 0.837860011864219 1103 80-81 0.9625776371640384 1103 82-83 0.8635698777966283 1103 84-85 0.8796495170472163 1103 86-87 1.0721364516465712 1103 88-89 1.2069540832751038 1103 90-91 1.066120717328804 1103 92-93 1.2528696457122912 1103 94-95 1.2388066682728436 1103 96-97 1.0911447772570213 1103 98-99 1.2386242913366665 1103 100-101 0.9786137766888103 1104 1 0.12930603028932808 1104 2 0.157172213488046 1104 3 -0.006272121069905268 1104 4 -0.026293455746802863 1104 5 0.018662840980979922 1104 6 0.02382831053346024 1104 7 -0.01678600166576416 1104 8 0.05857130990053605 1104 9 0.008409085415372886 1104 10-11 0.07476768184210414 1104 12-13 0.05159700331331152 1104 14-15 0.2245209024236985 1104 16-17 0.1894850560657133 1104 18-19 0.21167221930338798 1104 20-21 0.1072457840002059 1104 22-23 0.027851742000919444 1104 24-25 0.11706866855755749 1104 26-27 0.10972219664093785 1104 28-29 0.1879995576249911 1104 30-31 0.08088033249499205 1104 32-33 -0.028078853670315596 1104 34-35 0.13400491637343492 1104 36-37 0.1292131300373569 1104 38-39 0.06877805653044078 1104 40-41 0.024053862281725458 1104 42-43 0.09663110143979026 1104 44-45 0.07775572320863233 1104 46-47 0.1401467821313389 1104 48-49 0.34392678391734677 1104 50-51 0.29840034767008916 1104 52-53 0.2864342187089832 1104 54-55 0.46766339667761514 1104 56-57 0.5431205825513103 1104 58-59 0.5608275555406976 1104 60-61 0.6168583521789301 1104 62-63 0.6000716081503654 1104 64-65 0.6299825238142418 1104 66-67 0.6570190162257106 1104 68-69 0.6577144253795026 1104 70-71 0.7048081247403246 1104 72-73 0.8155916161611501 1104 74-75 0.7078632656280952 1104 76-77 0.6324880582408632 1104 78-79 0.5776633808373397 1104 80-81 0.6492889036566254 1104 82-83 0.7657672011392442 1104 84-85 0.8720899745543882 1104 86-87 0.6763196748994744 1104 88-89 0.7859557127771026 1104 90-91 0.9007468556984541 1104 92-93 1.0619530063152958 1104 94-95 1.181761298217836 1104 96-97 1.1000995251850796 1104 98-99 1.3134450349861737 1104 100-101 1.4417376678149267 1105 1 0.13513149740058594 1105 2 0.11761834031907625 1105 3 0.21886465113426112 1105 4 0.21740374849123612 1105 5 0.169257986800865 1105 6 0.21574966052655498 1105 7 0.15434146354575518 1105 8 0.19735655287730935 1105 9 0.1997063990148007 1105 10-11 0.24609498526641715 1105 12-13 0.31589248200896947 1105 14-15 0.3519060659161539 1105 16-17 0.3051860259434136 1105 18-19 0.38983911944347227 1105 20-21 0.31302671734894716 1105 22-23 0.3517080276032658 1105 24-25 0.29813759160152387 1105 26-27 0.45100189403684254 1105 28-29 0.476306344514029 1105 30-31 0.3692215062133073 1105 32-33 0.5886762698263155 1105 34-35 0.5143308584175301 1105 36-37 0.5789652348213536 1105 38-39 0.5503681373394826 1105 40-41 0.4672008772512726 1105 42-43 0.4730096045980261 1105 44-45 0.5727611771852423 1105 46-47 0.7716758404113762 1105 48-49 0.9532887391186335 1105 50-51 0.9629334337189093 1105 52-53 0.9734577004036353 1105 54-55 1.0432605859771158 1105 56-57 1.1204278119593738 1105 58-59 1.1560641341075524 1105 60-61 1.1865762392491987 1105 62-63 1.0838793575887635 1105 64-65 1.2581033399659063 1105 66-67 1.3464357648664418 1105 68-69 1.2655385564778854 1105 70-71 1.3577190262107806 1105 72-73 1.3628749157183435 1105 74-75 1.3984649505827313 1105 76-77 1.1880709854619802 1105 78-79 1.3177119170012936 1105 80-81 1.4029780185340037 1105 82-83 1.4988822814550389 1105 84-85 1.562309458357344 1105 86-87 1.551191076613744 1105 88-89 1.797772943960883 1105 90-91 1.9505993023777286 1105 92-93 1.9441597951773453 1105 94-95 2.202269941431936 1105 96-97 1.9979280814737805 1105 98-99 2.0356332775091524 1105 100-101 2.2461011309632397 1106 1 0.05854008238142683 1106 2 -0.501049596715383 1106 3 -0.09100275422775184 1106 4 0.02119897226122447 1106 5 0.03772081236937197 1106 6 0.046645475664519154 1106 7 0.09353260390669504 1106 8 0.10939122921288913 1106 9 0.16466071576572006 1106 10-11 -0.1611737533936477 1106 12-13 -0.15069772733705378 1106 14-15 -0.07135636795561595 1106 16-17 0.04593424904587806 1106 18-19 0.15324017631889575 1106 20-21 0.017343321232388575 1106 22-23 -0.03049917761700982 1106 24-25 0.06413466857933514 1106 26-27 0.2190856677349302 1106 28-29 0.18219708700283377 1106 30-31 0.2206020019508088 1106 32-33 0.12363961442240878 1106 34-35 0.32999696341695284 1106 36-37 0.30078733039889016 1106 38-39 0.38128189054113903 1106 40-41 0.43457536226711113 1106 42-43 0.15266842850799378 1106 44-45 0.34071467937162225 1106 46-47 0.22894619643783187 1106 48-49 0.2806542845092892 1106 50-51 0.272291809765413 1106 52-53 0.34196875289746487 1106 54-55 0.3777443079194853 1106 56-57 0.2750459811594226 1106 58-59 0.15682459038131924 1106 60-61 0.2699911760689844 1106 62-63 0.3284416247247606 1106 64-65 0.32819500053069106 1106 66-67 0.44102905603062226 1106 68-69 0.497147777230758 1106 70-71 0.4741852864773861 1106 72-73 0.4218411539299538 1106 74-75 0.4444746136117921 1106 76-77 0.4700097078601537 1106 78-79 0.3922572164934408 1106 80-81 0.4077545426077691 1106 82-83 0.27679023272231973 1106 84-85 0.10776539394499451 1106 86-87 0.3730925701780947 1106 88-89 0.344124606466508 1106 90-91 0.4947401720503457 1106 92-93 0.6936282697655791 1106 94-95 0.7440026995709665 1106 96-97 0.5940737503136049 1106 98-99 0.7785499478516549 1106 100-101 0.6136435280178674 1107 1 0.06857171078234359 1107 2 0.15738906842758382 1107 3 0.07973520331631789 1107 4 0.041159494476140424 1107 5 0.017220644992796963 1107 6 0.1086700396196818 1107 7 0.09445836951023523 1107 8 0.06806479872033577 1107 9 0.17897545298449558 1107 10-11 0.10881418394979647 1107 12-13 0.1445060680710597 1107 14-15 0.17541210984101951 1107 16-17 0.28336830844724403 1107 18-19 0.1758316851321382 1107 20-21 0.27727250362669054 1107 22-23 0.34848034472487655 1107 24-25 0.27555090526136183 1107 26-27 0.21727367202900183 1107 28-29 0.28188917182235684 1107 30-31 0.3640018819014088 1107 32-33 0.284734949810435 1107 34-35 0.3722546425387421 1107 36-37 0.3619571195044031 1107 38-39 0.4302600276975781 1107 40-41 0.4290943566224783 1107 42-43 0.43222020558548024 1107 44-45 0.4420421487532522 1107 46-47 0.4715481518579523 1107 48-49 0.46105362192891874 1107 50-51 0.33150502443452723 1107 52-53 0.47462392252496954 1107 54-55 0.5710697341525801 1107 56-57 0.6070786830790666 1107 58-59 0.7195111575454973 1107 60-61 0.6599687822376765 1107 62-63 0.7146976993400784 1107 64-65 0.5479875690107932 1107 66-67 0.8197658823399081 1107 68-69 0.7590335340614516 1107 70-71 0.7381202520208987 1107 72-73 0.8438422274486683 1107 74-75 0.8479811528414025 1107 76-77 0.8723766434836584 1107 78-79 0.9056808177447273 1107 80-81 0.9001319825542069 1107 82-83 1.0156775825162754 1107 84-85 0.784430790858341 1107 86-87 1.1209005142166006 1107 88-89 1.0856013872955437 1107 90-91 1.1179550143123542 1107 92-93 1.446531964747745 1107 94-95 1.2111230144697878 1107 96-97 1.417775138962007 1107 98-99 1.7655762432886206 1107 100-101 1.8683430582091383 1108 1 0.05130051956859916 1108 2 0.08027276316146015 1108 3 0.09813002167734197 1108 4 0.05524003516890019 1108 5 0.032131937586434844 1108 6 0.07401782897479592 1108 7 0.11215479305289477 1108 8 1.4871874286370712E-4 1108 9 0.1692824087716005 1108 10-11 0.1821836573375819 1108 12-13 0.10731654399333479 1108 14-15 0.2106979448170847 1108 16-17 0.14858655682798627 1108 18-19 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56-57 -0.11218130440865082 2316 58-59 -0.16802570901987224 2316 60-61 -0.22404453441260586 2316 62-63 -0.10129345268423151 2316 64-65 -0.11089839041364513 2316 66-67 -0.08058490861549927 2316 68-69 -0.0010947168993098444 2316 70-71 0.12515886136936416 2316 72-73 0.26457911188874306 2316 74-75 0.2947190203898806 2316 76-77 0.33374048296886727 2316 78-79 0.338563559723454 2316 80-81 0.14177779540611368 2316 82-83 0.3644397596545339 2316 84-85 0.4840308444824366 2316 86-87 0.6154911836240586 2316 88-89 0.6925032552015189 2316 90-91 0.7614642644687564 2316 92-93 0.952535629060911 2316 94-95 1.2215551353027472 2316 96-97 1.259065692183924 2316 98-99 1.6044983989772454 2316 100-101 2.2275798085889775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4003.0 3 421.0 4 372.0 5 346.0 6 407.0 7 468.0 8 563.0 9 634.0 10 927.0 11 1466.0 12 2404.0 13 3913.0 14 4303.0 15 6358.0 16 9605.0 17 15265.0 18 12038.0 19 5810.0 20 4979.0 21 4804.0 22 5228.0 23 5855.0 24 6779.0 25 7973.0 26 9267.0 27 11013.0 28 13572.0 29 17204.0 30 21666.0 31 27629.0 32 36831.0 33 51277.0 34 74198.0 35 111956.0 36 166351.0 37 243372.0 38 190960.0 39 20347.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.516700843016636 22.99339257233994 16.027067669172933 21.462838915470495 2 13.911401261583647 22.60920424026208 41.872588068523456 21.60680642963082 3 16.575824796505405 28.35086248912494 32.44188959592601 22.631423118443646 4 10.452951197514782 19.461505525239367 41.97341690276854 28.112126374477313 5 10.635613293491728 40.012025362582904 36.84789738357262 12.504463960352744 6 25.79128982404246 41.71835683107038 19.428367950792726 13.061985394094435 7 24.397303153030492 35.49415631548702 23.84777003760395 16.260770493878542 8 23.805585938887457 36.006236754410494 21.920352056043356 18.267825250658692 9 25.16693278149043 16.944121352002966 23.63236259867729 34.25658326782931 10-11 21.702749405402255 28.935708748671207 30.27989590098752 19.08164594493902 12-13 23.004104712289863 27.85848602471389 29.78204902246092 19.355360240535322 14-15 20.766543731753863 28.635759442225524 29.029746180919698 21.567950645100918 16-17 18.079194575863685 31.761113969700972 29.97923601276803 20.180455441667313 18-19 17.798291283176674 30.21177981091657 33.546850848098124 18.443078057808627 20-21 18.899945589196154 29.320684528891228 33.4616426598915 18.317727222021123 22-23 18.674338936069077 29.2222675508171 33.04099425035104 19.06239926276278 24-25 18.25863454546696 29.715312132188053 33.433715876727085 18.5923374456179 26-27 18.164976381537763 29.745466962608845 33.54732229241363 18.542234363439757 28-29 17.761084199204575 29.46421171792679 33.99716036126616 18.777543721602473 30-31 18.277320205031046 29.370993708343107 33.913920660908616 18.437765425717235 32-33 18.05104095608212 29.685030979588902 33.44383919211717 18.820088872211816 34-35 18.453951188191176 29.64564876353891 33.29854256745834 18.601857480811574 36-37 18.640194407550094 29.15202592100668 33.071685735113796 19.13609393632943 38-39 17.76366528597145 30.355368913323964 33.07476787839441 18.806197922310172 40-41 18.03509155307893 29.91422874156628 33.519853952124706 18.53082575323008 42-43 17.997596835727467 29.65817970550993 32.942064071328176 19.402159387434427 44-45 18.41558778601625 29.760532887742535 32.61424145223748 19.20963787400374 46-47 18.613342100445976 29.945229831618615 32.200697058311924 19.240731009623484 48-49 18.239989508297054 30.221796603948686 32.12337222640403 19.41484166135023 50-51 18.48188305634586 30.13434852646551 31.814809535152005 19.56895888203663 52-53 18.387989516652837 30.7890476897556 31.493813203660974 19.329149589930594 54-55 18.79206855834486 30.256255894322738 31.470070843440865 19.481604703891538 56-57 18.817067718534343 30.73225542392034 30.910359057289092 19.54031780025623 58-59 18.719491015796958 30.224487854220598 31.52221365882927 19.533807471153175 60-61 18.927532827468912 30.826044915953304 31.104698274998682 19.141723981579105 62-63 18.666364977739818 30.530388254527725 31.5384815000799 19.26476526765256 64-65 18.724453029081495 30.19011794569008 31.409817229475816 19.675611795752605 66-67 18.7001134021698 30.463073276193693 30.80683071664617 20.02998260499033 68-69 18.722522413424976 30.076168996746283 31.292614871506075 19.90869371832267 70-71 18.88615632836131 30.25987045174355 31.509781676406156 19.34419154348899 72-73 19.022561833683362 29.66264010376522 31.586567156704515 19.7282309058469 74-75 18.554232226107224 30.524976325757574 31.01375837703963 19.90703307109557 76-77 18.499551500084234 30.2063554942378 31.450271148933844 19.843821856744118 78-79 18.429963887903607 30.060554125571382 31.236348685034308 20.273133301490702 80-81 18.606316703902397 30.224457023803396 30.881951877805484 20.287274394488716 82-83 18.741369914651784 30.206266153311745 31.090660682685563 19.961703249350908 84-85 18.625491343030838 29.79422201080215 30.995705087206986 20.584581558960025 86-87 18.375920915106466 30.040132415119214 31.25330036308546 20.330646306688855 88-89 18.916667235739602 29.513533692532167 31.255761863463473 20.31403720826476 90-91 18.481897466551274 29.44134289308199 31.61768957160957 20.459070068757168 92-93 18.85503582366661 29.737809094936246 31.415559540768683 19.99159554062846 94-95 18.672486681435227 29.08641611102461 31.52555042935356 20.7155467781866 96-97 19.00416456662692 28.957191970267537 31.255102129087454 20.783541334018093 98-99 18.753548557470126 29.078797895565984 31.056747324495802 21.11090622246809 100-101 19.007334413472268 28.22007883891286 31.91378069416302 20.858806053451854 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2359.0 1 1401.5 2 535.5 3 642.5 4 549.5 5 551.5 6 1040.5 7 2222.5 8 3463.5 9 3767.0 10 3024.5 11 2273.0 12 2199.0 13 2217.0 14 2154.5 15 2141.0 16 2170.5 17 2295.0 18 2466.5 19 2676.0 20 3119.5 21 3694.5 22 4284.0 23 4958.5 24 5718.0 25 7295.5 26 10511.0 27 14011.5 28 15392.0 29 17267.0 30 21579.5 31 25830.5 32 31665.0 33 39719.0 34 47653.0 35 52307.5 36 57063.0 37 62320.5 38 60021.0 39 55058.0 40 50969.5 41 46610.0 42 42731.5 43 41795.0 44 41533.0 45 39268.5 46 35877.0 47 31958.0 48 30895.0 49 28282.5 50 23149.0 51 18948.0 52 16023.0 53 13790.5 54 11916.0 55 10228.5 56 8443.5 57 6920.5 58 5591.5 59 4371.0 60 3431.0 61 2711.5 62 2022.0 63 1434.0 64 967.0 65 632.5 66 432.0 67 304.0 68 212.5 69 147.0 70 115.0 71 78.5 72 68.0 73 66.0 74 51.5 75 42.5 76 30.0 77 21.5 78 17.5 79 8.0 80 8.5 81 8.5 82 4.0 83 2.0 84 1.5 85 2.5 86 2.5 87 1.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3012995131595924 2 0.26331905583127224 3 0.2608657727024095 4 0.26132008439293963 5 0.2622287077739999 6 0.22870050501287517 7 0.23106292580363197 8 0.23251672321332845 9 0.24251158040499163 10-11 0.2512343648631704 12-13 0.24364735963131695 14-15 0.23256215438238145 16-17 0.228155330984239 18-19 0.21566175949466002 20-21 0.2215678114715519 22-23 0.21915995951174214 24-25 0.22111349978102177 26-27 0.21611607118519016 28-29 0.23069947645120784 30-31 0.24382908430752903 32-33 0.23487914400408516 34-35 0.23383422711586582 36-37 0.22356678290988452 38-39 0.21143666077272968 40-41 0.20861992829144274 42-43 0.22084091276670367 44-45 0.21793331794731072 46-47 0.22924567904151136 48-49 0.23219870502995732 50-51 0.2464640921126039 52-53 0.25668610514953216 54-55 0.23456112582071406 56-57 0.21161838544894174 58-59 0.20185068410254361 60-61 0.20425853606235336 62-63 0.20939225816534401 64-65 0.20185068410254361 66-67 0.2055760399648908 68-69 0.20757501140322346 70-71 0.2078930295865945 72-73 0.2105734685607224 74-75 0.21134579843462364 76-77 0.22361221407893755 78-79 0.22329419589556646 80-81 0.2164795205376143 82-83 0.20902880881291988 84-85 0.1974892918734542 86-87 0.20007886850947604 88-89 0.20793846075564754 90-91 0.20721156205079932 92-93 0.21257243999905506 94-95 0.20780216724848852 96-97 0.215570897156554 98-99 0.21884194132837104 100-101 0.20448569190761845 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1100566.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.67910485604974 #Duplication Level Percentage of deduplicated Percentage of total 1 78.42304568458216 32.68602344218479 2 12.430376618537103 10.361739409683938 3 3.739374778638134 4.675613804847798 4 1.5610983195131678 2.602607221983695 5 0.8042966030460499 1.6761181226860464 6 0.459471259052011 1.1490210470621978 7 0.31091037620067935 0.9070926319351391 8 0.2186267251499997 0.7289732961489284 9 0.1655901375844373 0.6211483836758519 >10 1.392542757763646 12.326883829027667 >50 0.2593425907098097 7.671286798618206 >100 0.21829698445917722 17.36643274048038 >500 0.013752710001402267 3.725804174321379 >1k 0.0026195638097909076 1.4122572013141987 >5k 4.365939682984847E-4 1.1769719853240868 >10k+ 2.1829698414924234E-4 0.9120259107056777 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10024 0.9108040771748356 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7142 0.6489388187532596 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5794 0.5264563869863325 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1816 0.1650060060005488 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1634 0.14846906046525152 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1624 0.14756043708419123 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1503 0.13656609417336169 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1285 0.11675810446624736 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1201 0.10912566806534091 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1181 0.10730842130322034 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 9.086233810602908E-5 0.0 24-25 0.0 0.0 0.0 3.1801818337110177E-4 0.0 26-27 0.0 0.0 0.0 6.81467535795218E-4 0.0 28-29 0.0 0.0 0.0 0.0018626779311735962 0.0 30-31 0.0 0.0 0.0 0.00540630911730873 0.0 32-33 0.0 0.0 0.0 0.011221498756094591 0.0 34-35 0.0 0.0 0.0 0.020080576721432427 0.0 36-37 0.0 0.0 0.0 0.03470941315650311 0.0 38-39 0.0 0.0 0.0 0.057743015866381475 0.0 40-41 0.0 0.0 0.0 0.08541059781966734 0.0 42-43 0.0 0.0 0.0 0.1143502525064376 0.0 44-45 0.0 0.0 0.0 0.1552837358232037 0.0 46-47 0.0 0.0 0.0 0.21007372570113925 0.0 48-49 0.0 0.0 0.0 0.2682256220889978 0.0 50-51 0.0 0.0 0.0 0.3329650379895436 0.0 52-53 0.0 0.0 0.0 0.41192440980368283 0.0 54-55 0.0 0.0 0.0 0.5052400310385747 0.0 56-57 0.0 0.0 0.0 0.6181364861353158 0.0 58-59 0.0 0.0 0.0 0.7419818529738335 0.0 60-61 0.0 0.0 0.0 0.8866801264076848 0.0 62-63 0.0 0.0 0.0 1.0359215167468374 0.0 64-65 0.0 0.0 0.0 1.2082419409649217 0.0 66-67 0.0 0.0 0.0 1.409184001686405 0.0 68-69 0.0 0.0 0.0 1.6425639171117408 0.0 70-71 0.0 0.0 0.0 1.9232831106903174 0.0 72-73 0.0 0.0 0.0 2.1985959951515857 0.0 74-75 0.0 0.0 0.0 2.5112078694053785 0.0 76-77 0.0 0.0 0.0 2.8605735594230604 0.0 78-79 0.0 0.0 0.0 3.2649109639948897 0.0 80-81 0.0 0.0 0.0 3.7055478726400777 0.0 82-83 0.0 0.0 0.0 4.1549984280815515 0.0 84-85 0.0 0.0 0.0 4.614080391362263 0.0 86-87 0.0 0.0 0.0 5.128134069197122 0.0 88-89 0.0 0.0 0.0 5.694887903133479 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGAG 45 9.6207974E-4 42.22587 7 CATTCCG 270 0.0 42.225864 9 TAGGACG 60 8.272962E-5 39.597572 4 GGTATCA 4300 0.0 38.36791 1 GTATCAA 7115 0.0 36.352234 1 AGATCGA 60 0.0039492073 31.669397 6 GTACATG 5375 0.0 29.279028 1 ATCAACG 8810 0.0 29.177721 3 TCAACGC 8855 0.0 28.977104 4 CAACGCA 8935 0.0 28.715038 5 TATCAAC 9140 0.0 28.076109 2 AACGCAG 9205 0.0 27.867695 6 ACATGGG 5320 0.0 27.151384 3 TACATGG 5755 0.0 26.836594 2 CCCTATA 595 0.0 26.356415 2 GACAATG 610 0.0 25.698938 7 GGACAAT 655 0.0 25.38387 6 ATGGGTA 470 0.0 25.272743 5 TTAGGCA 465 0.0 24.524946 4 ACGCAGA 10345 0.0 24.475288 7 >>END_MODULE