FastQCFastQC Report
Thu 26 May 2016
SRR937191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100566
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99580.9048071628598376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83450.7582462114948126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65010.590696060027295No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23320.21189097246325983No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21140.19208298275614547No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18290.1661872163959272No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16570.1505588942416902No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT13320.12102863435723074No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT13110.11912052525700412No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA11980.10885308105102284No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCG502.827852E-547.497345
GGTATCA68800.031.6260531
ACTCCCG3250.029.2304636
GTATCAA98300.028.2728961
CTCCCGC3550.028.1034057
GGTACTC3750.026.598513
ACCACCG950.001226921824.99865
GTACATG47200.024.6599431
CATTCCG2551.8189894E-1124.2198339
TCGCTGA500.001654226623.7497526-27
GAGTACT76000.023.65976312-13
GACATAT5500.023.3221741
GACAATG6950.023.2413887
GTGGTAC4300.023.2016451
ATCAACG121050.022.8363913
TCAACGC121800.022.6957744
CAACGCA123100.022.5332645
TATCAAC124550.022.23282
AACGCAG125150.022.2031216
AGTACTT80750.022.09151512-13
Produced by FastQC (version 0.11.5)