##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937191_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1100566 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.420395505585308 31.0 31.0 34.0 30.0 34.0 2 31.675150786050086 31.0 31.0 34.0 30.0 34.0 3 31.682209881097545 31.0 31.0 34.0 30.0 34.0 4 35.516426093482806 37.0 35.0 37.0 33.0 37.0 5 35.41831475804268 37.0 35.0 37.0 33.0 37.0 6 35.479128012313666 37.0 35.0 37.0 33.0 37.0 7 35.394884086915276 37.0 35.0 37.0 33.0 37.0 8 35.36601803072237 37.0 35.0 37.0 33.0 37.0 9 36.899623466470885 39.0 37.0 39.0 33.0 39.0 10-11 36.78446635640206 39.0 36.0 39.0 32.5 39.0 12-13 36.6601289700027 38.0 35.0 39.0 32.0 39.0 14-15 37.579109748983704 39.0 36.0 40.5 32.0 41.0 16-17 37.5088372710042 39.0 36.0 40.0 32.0 41.0 18-19 37.571851211104104 39.0 36.5 40.0 32.0 41.0 20-21 37.47784003867101 39.0 36.0 40.0 32.0 41.0 22-23 37.37213579194706 39.0 36.0 40.0 32.0 41.0 24-25 37.38441129382518 39.0 36.0 40.0 32.0 41.0 26-27 37.136055902144896 39.0 36.0 40.0 31.5 41.0 28-29 36.940451549475455 39.0 36.0 40.0 31.0 41.0 30-31 36.864656912897544 39.0 36.0 40.0 31.0 41.0 32-33 36.65467268659944 38.0 35.0 40.0 30.5 41.0 34-35 36.431452089197734 38.0 35.0 40.0 30.0 41.0 36-37 36.15727498396279 38.0 35.0 40.0 30.0 41.0 38-39 35.974623057590364 38.0 35.0 40.0 30.0 41.0 40-41 35.717078757657426 38.0 35.0 40.0 29.0 41.0 42-43 35.40985729161177 38.0 34.5 40.0 28.0 41.0 44-45 34.90391216882949 38.0 34.0 40.0 26.5 41.0 46-47 34.36411310180398 38.0 33.0 40.0 24.0 41.0 48-49 33.890838441311104 38.0 33.0 40.0 23.0 41.0 50-51 33.57819976266757 38.0 33.0 40.0 21.5 41.0 52-53 33.2919920295557 37.0 33.0 40.0 20.0 41.0 54-55 33.016594188808305 37.0 32.0 40.0 18.5 40.0 56-57 32.61403405156983 37.0 31.0 39.0 16.5 40.0 58-59 32.30593440102638 36.0 31.0 39.0 13.5 40.0 60-61 31.86210777000198 36.0 30.5 39.0 11.0 40.0 62-63 31.402393404848052 35.5 29.5 39.0 8.0 40.0 64-65 30.892649327709563 35.0 29.0 38.0 7.5 40.0 66-67 30.307779360801625 34.5 28.0 38.0 6.0 40.0 68-69 29.71612606604238 34.0 28.0 37.5 2.0 39.0 70-71 29.19166547031255 34.0 26.5 37.0 2.0 39.0 72-73 28.592174844579972 33.0 26.0 36.0 2.0 39.0 74-75 27.97020260484151 32.5 26.0 36.0 2.0 38.5 76-77 26.917744596871067 31.0 25.0 34.5 2.0 36.5 78-79 27.524789517393778 32.0 26.0 35.0 2.0 37.0 80-81 27.21894098127691 32.0 26.0 35.0 2.0 37.0 82-83 26.627649318623327 32.0 24.5 35.0 2.0 36.0 84-85 25.944646663625804 31.5 23.0 34.0 2.0 36.0 86-87 25.321932078585018 31.0 20.5 34.0 2.0 35.5 88-89 24.549897961594308 31.0 18.5 34.0 2.0 35.0 90-91 23.63566610271442 30.0 9.5 34.0 2.0 35.0 92-93 22.764802837812542 29.0 3.5 34.0 2.0 35.0 94-95 21.841432044965956 29.0 2.0 34.0 2.0 35.0 96-97 20.683435614038594 29.0 2.0 33.5 2.0 35.0 98-99 19.217388598230364 26.5 2.0 33.0 2.0 34.0 100-101 16.418982141916068 20.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2787930516413155 1101 2 0.3615233686236614 1101 3 0.3090436469928335 1101 4 0.25377320303568496 1101 5 0.18606356985859662 1101 6 0.3524256581405112 1101 7 0.2660063860377093 1101 8 0.280134465036177 1101 9 0.4727109424063727 1101 10-11 0.4431001899204787 1101 12-13 0.3498629789694405 1101 14-15 0.617354459675532 1101 16-17 0.4151221824949829 1101 18-19 0.4060895278665271 1101 20-21 0.6076662239645785 1101 22-23 0.5103760412197715 1101 24-25 0.40909390244546273 1101 26-27 0.343515942246583 1101 28-29 0.3879910700070681 1101 30-31 0.5510469591634006 1101 32-33 0.47268967098305126 1101 34-35 0.48926560400033736 1101 36-37 0.6114676894741677 1101 38-39 0.6551800985942791 1101 40-41 0.7162814856683326 1101 42-43 0.8687532057846781 1101 44-45 0.720561803157743 1101 46-47 0.6674775987438366 1101 48-49 0.6901581060098252 1101 50-51 0.6668768984358593 1101 52-53 0.8662427636975139 1101 54-55 0.8627959636533689 1101 56-57 0.9157513320345245 1101 58-59 1.0489291436372952 1101 60-61 1.0381933663289367 1101 62-63 0.9855022568029774 1101 64-65 1.1701861927611645 1101 66-67 1.140591993528151 1101 68-69 1.2510641827027342 1101 70-71 1.182211310620346 1101 72-73 1.3156459271238852 1101 74-75 1.3627657899523875 1101 76-77 1.2646176678355623 1101 78-79 1.4793040902192978 1101 80-81 1.365521454829377 1101 82-83 1.3876874535112265 1101 84-85 1.6038635682595341 1101 86-87 2.0807314782403274 1101 88-89 2.276682940310085 1101 90-91 2.613301747917429 1101 92-93 2.4412871837143335 1101 94-95 2.618112867144614 1101 96-97 3.1068057274942014 1101 98-99 3.301947674247703 1101 100-101 3.6051829483585287 1102 1 0.4147082367018733 1102 2 0.4052195895838402 1102 3 0.3049473408789325 1102 4 0.2853006822794626 1102 5 0.25969979406926313 1102 6 0.2217272831573922 1102 7 0.28524151332192105 1102 8 0.3101992230691266 1102 9 0.33860184034322316 1102 10-11 0.24113936521124657 1102 12-13 0.27056128528020906 1102 14-15 0.4419217665974742 1102 16-17 0.3546618945746829 1102 18-19 0.48582843556401656 1102 20-21 0.5033935443463022 1102 22-23 0.5168667235254247 1102 24-25 0.2786753673184137 1102 26-27 0.28952802400331734 1102 28-29 0.34004517466831885 1102 30-31 0.43266611600419225 1102 32-33 0.6474791329809122 1102 34-35 0.7440783917089036 1102 36-37 0.7952303394946583 1102 38-39 0.6800932852892387 1102 40-41 0.6648189933653654 1102 42-43 0.7685905667517758 1102 44-45 0.8918009042823627 1102 46-47 1.0321970607188078 1102 48-49 1.0901001923218274 1102 50-51 1.0769813490913407 1102 52-53 1.2345114420064363 1102 54-55 1.3020455350234101 1102 56-57 1.3731132376466562 1102 58-59 1.6121471989734815 1102 60-61 1.60843317020068 1102 62-63 1.5340238554038912 1102 64-65 1.5344987710190452 1102 66-67 1.6285886377389573 1102 68-69 1.5640613419562186 1102 70-71 1.4704835598918464 1102 72-73 1.5362198220139796 1102 74-75 1.4272474166631888 1102 76-77 1.506208165412577 1102 78-79 1.8068351816290722 1102 80-81 1.8789886763686923 1102 82-83 1.8025068337660919 1102 84-85 2.0387015129416604 1102 86-87 2.5991728675279013 1102 88-89 2.6532581125060197 1102 90-91 3.1332642299195506 1102 92-93 2.898855678668056 1102 94-95 3.0064825167095712 1102 96-97 3.1817398592581405 1102 98-99 3.7546530217641667 1102 100-101 3.9196039144870305 1103 1 0.1825612915504422 1103 2 0.17787340932286 1103 3 0.2604207515092938 1103 4 0.18894535834566284 1103 5 0.1695390054779864 1103 6 0.09415015445918584 1103 7 0.16055292338669602 1103 8 0.10891385698555212 1103 9 0.07595354182048197 1103 10-11 0.16077536892796473 1103 12-13 0.3616697704469871 1103 14-15 0.23421461998898252 1103 16-17 0.2884965139474147 1103 18-19 0.19465834170333807 1103 20-21 0.3791931107288349 1103 22-23 0.3539336110407447 1103 24-25 0.3051149234664834 1103 26-27 0.26388635094011903 1103 28-29 0.29982614397843577 1103 30-31 0.2689469035181702 1103 32-33 0.3929359126305201 1103 34-35 0.4798208606352219 1103 36-37 0.4155389889512833 1103 38-39 0.5606952998598587 1103 40-41 0.5648344797037126 1103 42-43 0.5945072906505615 1103 44-45 0.7680717940061754 1103 46-47 0.5587796971669192 1103 48-49 0.7326502535938673 1103 50-51 0.5686860582848396 1103 52-53 0.6746213950087636 1103 54-55 0.6986540269233785 1103 56-57 0.7779816711025376 1103 58-59 0.5951526259250954 1103 60-61 0.7564859314821426 1103 62-63 0.845955742447881 1103 64-65 1.0162753906880369 1103 66-67 0.8413574603732492 1103 68-69 0.8264335796647799 1103 70-71 0.8914224359876712 1103 72-73 0.6868059462548111 1103 74-75 0.9396752031941169 1103 76-77 1.107392870296831 1103 78-79 0.9827094864271437 1103 80-81 1.1858925533398335 1103 82-83 0.9202450877151094 1103 84-85 1.0704350401908833 1103 86-87 1.294332713472496 1103 88-89 1.4968006460709766 1103 90-91 1.7721665851896198 1103 92-93 1.9205921011892322 1103 94-95 2.0073443651050518 1103 96-97 2.244587460222334 1103 98-99 2.4447478250876706 1103 100-101 2.5486438288408486 1104 1 0.13467574848328567 1104 2 0.1529433643779896 1104 3 0.23049757406943883 1104 4 0.19817419429799799 1104 5 -0.03174623083319972 1104 6 0.05311597721299677 1104 7 0.05308648734353483 1104 8 0.09661300906572734 1104 9 0.03236547636436882 1104 10-11 0.07329815858196298 1104 12-13 0.06276272774825031 1104 14-15 0.09670618865178682 1104 16-17 0.07191605283396285 1104 18-19 0.20206567490755845 1104 20-21 0.23563776887845478 1104 22-23 0.17578641269339812 1104 24-25 0.04604720118527439 1104 26-27 0.1451249839393185 1104 28-29 0.1490752262705115 1104 30-31 0.0734264200992385 1104 32-33 0.16181904706220962 1104 34-35 0.002096843066880183 1104 36-37 0.4003993125810723 1104 38-39 0.44188217613569236 1104 40-41 0.5088977052717567 1104 42-43 0.43750187901208193 1104 44-45 0.253782486166088 1104 46-47 0.6426506325945596 1104 48-49 0.7351281762904662 1104 50-51 0.8810389968423706 1104 52-53 0.7434080281949491 1104 54-55 0.8043796561849632 1104 56-57 0.5953602988036266 1104 58-59 0.720437844427515 1104 60-61 1.0587335089335745 1104 62-63 0.8849101898530343 1104 64-65 0.9739612084553961 1104 66-67 1.2431193037585153 1104 68-69 1.1364880744857686 1104 70-71 1.1840866717579104 1104 72-73 1.1780671296027236 1104 74-75 1.3754693182877098 1104 76-77 1.2688028903984758 1104 78-79 1.4011136207319446 1104 80-81 1.3737062936924893 1104 82-83 1.1925363745542086 1104 84-85 1.1820225357665173 1104 86-87 1.4616040433642858 1104 88-89 1.8603763233049193 1104 90-91 2.1311395314494455 1104 92-93 2.488677514252405 1104 94-95 2.6854882243089584 1104 96-97 3.2053569769592336 1104 98-99 3.482195762276156 1104 100-101 3.6378899504257376 1105 1 0.28947961671972067 1105 2 0.1555392881294253 1105 3 0.2386461251719325 1105 4 0.3117972977375274 1105 5 0.19503998173941284 1105 6 0.03890688837941525 1105 7 0.06319339655949108 1105 8 0.21354795876793986 1105 9 0.2735203101096886 1105 10-11 0.26382233224108376 1105 12-13 0.29416072147405714 1105 14-15 0.19923277502615377 1105 16-17 0.3486995877436456 1105 18-19 0.38972108505505787 1105 20-21 0.4104905678817943 1105 22-23 0.31615457725411034 1105 24-25 0.3720216590994596 1105 26-27 0.30967272666945433 1105 28-29 0.4708246139312777 1105 30-31 0.32009301160343284 1105 32-33 0.2882033283352001 1105 34-35 0.34366093645248696 1105 36-37 0.2181423527713804 1105 38-39 0.5046336298248022 1105 40-41 0.38571562701071826 1105 42-43 0.2882717775709338 1105 44-45 0.4199929166497327 1105 46-47 0.5833866234848699 1105 48-49 0.7422755633142302 1105 50-51 0.7106144886163079 1105 52-53 0.8618509249189046 1105 54-55 0.8320199023044665 1105 56-57 1.0584807062593669 1105 58-59 1.1099741013929574 1105 60-61 1.0209483025985087 1105 62-63 1.1911652104710377 1105 64-65 1.4087330574803048 1105 66-67 1.4603865655240291 1105 68-69 1.3726210218598247 1105 70-71 1.5957539538172867 1105 72-73 1.4341737513222377 1105 74-75 1.652309225048672 1105 76-77 1.7319235797134738 1105 78-79 1.6337154353418377 1105 80-81 1.6042494082936472 1105 82-83 1.6599249767295987 1105 84-85 1.7080536473912389 1105 86-87 2.1463106419713363 1105 88-89 2.3485393619731916 1105 90-91 2.5913646806234567 1105 92-93 2.769377483529748 1105 94-95 2.8667998281726845 1105 96-97 3.1601174388701843 1105 98-99 3.155381081918648 1105 100-101 3.007660545176954 1106 1 -0.15539847796384976 1106 2 0.1100236570183526 1106 3 0.03401104714135528 1106 4 0.12591190726116963 1106 5 0.03254876697962317 1106 6 0.02799525245741563 1106 7 -0.053226401181490246 1106 8 -0.06245414674904026 1106 9 -0.006608138188987311 1106 10-11 -0.24365284118470498 1106 12-13 -0.34608700024482175 1106 14-15 -0.08765626481693545 1106 16-17 -0.06335214445577009 1106 18-19 0.02582129119575427 1106 20-21 0.18764541454076067 1106 22-23 0.1775768598580072 1106 24-25 0.30951926980818456 1106 26-27 0.26252855462872304 1106 28-29 0.13255243175112952 1106 30-31 0.15942177514152434 1106 32-33 0.22432617760546947 1106 34-35 -0.14430732787811706 1106 36-37 0.45987334651070455 1106 38-39 0.4492070714922818 1106 40-41 0.5419688066060289 1106 42-43 0.4489139014376775 1106 44-45 0.5630663244995446 1106 46-47 0.6755153423248359 1106 48-49 0.5857112612372362 1106 50-51 0.7331144077479905 1106 52-53 0.4900786736331 1106 54-55 0.5848133881407449 1106 56-57 0.7030712037906781 1106 58-59 0.7424443492028985 1106 60-61 0.72254537840006 1106 62-63 0.5459724645063666 1106 64-65 0.5646213004028553 1106 66-67 0.6203214591748356 1106 68-69 0.8272853595188785 1106 70-71 0.8536462607404971 1106 72-73 1.2636795135576122 1106 74-75 0.9664226583058415 1106 76-77 0.7558873624688154 1106 78-79 1.1351293076404652 1106 80-81 1.05104569952546 1106 82-83 1.035070760255401 1106 84-85 1.281561479855604 1106 86-87 1.4117381119103776 1106 88-89 1.5472951661130594 1106 90-91 1.5540952656101794 1106 92-93 1.7027202269948525 1106 94-95 1.416273595960039 1106 96-97 1.6619003647742154 1106 98-99 1.9499717264832626 1106 100-101 2.021969532734996 1107 1 0.21948926429931248 1107 2 0.2230865328941931 1107 3 0.20278550432180964 1107 4 0.14011294384160777 1107 5 0.10723565735374052 1107 6 0.14310358550216762 1107 7 0.0782975336686178 1107 8 0.09579924984109311 1107 9 0.2860763511970248 1107 10-11 0.06876246049714752 1107 12-13 0.08303479218514553 1107 14-15 0.33450035348366924 1107 16-17 0.3422569908422233 1107 18-19 0.31041514328103403 1107 20-21 0.33185995667925994 1107 22-23 0.4798087399009461 1107 24-25 0.3161834546842712 1107 26-27 0.35984014055062374 1107 28-29 0.4717794405585991 1107 30-31 0.4796994471065261 1107 32-33 0.46577554330597337 1107 34-35 0.48350402140793847 1107 36-37 0.6532524486796731 1107 38-39 0.4792321202623526 1107 40-41 0.69981386207116 1107 42-43 0.8635853239076923 1107 44-45 0.7732205995281163 1107 46-47 0.6794836100350068 1107 48-49 0.6805537541093045 1107 50-51 0.6850781966918973 1107 52-53 0.6704003829519323 1107 54-55 0.8946369501451059 1107 56-57 0.8785150402926192 1107 58-59 0.8896687838740931 1107 60-61 1.3142276284029428 1107 62-63 1.0092742848709015 1107 64-65 1.3280991547506034 1107 66-67 1.3575916049656023 1107 68-69 1.0891907398100003 1107 70-71 1.4099883900013879 1107 72-73 1.3533639221411455 1107 74-75 1.3858161202305546 1107 76-77 1.3229300492668479 1107 78-79 1.3262967303875222 1107 80-81 1.5876215211135758 1107 82-83 1.5210821187760217 1107 84-85 1.4489579661018688 1107 86-87 1.7520452767820736 1107 88-89 1.9184077008869842 1107 90-91 2.270324444541508 1107 92-93 2.566653461056564 1107 94-95 2.8650277948034066 1107 96-97 3.2018361267993605 1107 98-99 3.2686564351903087 1107 100-101 3.2457528781887 1108 1 0.1712042741556914 1108 2 0.1994678339361542 1108 3 0.15802628156765408 1108 4 0.05064800438793782 1108 5 0.17325649655396802 1108 6 -0.042565240180735486 1108 7 -0.011753156277876542 1108 8 0.20712282066621412 1108 9 0.20841090484388758 1108 10-11 0.05540565959599775 1108 12-13 0.14913166630510943 1108 14-15 0.15183913129273918 1108 16-17 0.2320556391103139 1108 18-19 0.16156411540156057 1108 20-21 0.27022943006703315 1108 22-23 0.3600931668229421 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1.8517603765613018 2316 100-101 2.1609664808341584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 8.0 5 26.0 6 92.0 7 247.0 8 547.0 9 1062.0 10 1792.0 11 2905.0 12 4351.0 13 6068.0 14 8069.0 15 11309.0 16 15029.0 17 19395.0 18 16341.0 19 11118.0 20 10616.0 21 11259.0 22 12385.0 23 14239.0 24 16421.0 25 19946.0 26 23348.0 27 28237.0 28 33909.0 29 40992.0 30 48627.0 31 57874.0 32 69168.0 33 83629.0 34 101705.0 35 123533.0 36 139073.0 37 124085.0 38 42058.0 39 1100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.08297596561999 25.188561082156095 17.34507450639626 19.38338844582766 2 15.570079395511039 25.215934346508977 39.353750706454676 19.860235551525307 3 17.722153873552333 29.015070427398264 33.210093715415525 20.052681983633875 4 12.02535786131863 21.79896525969365 40.655626286837865 25.520050592149857 5 10.695412137137955 40.818095580779165 36.6099498798789 11.876542402203977 6 25.86160212109042 43.10209474034269 18.28659071786699 12.749712420699893 7 24.19991168180736 36.3836425984448 23.346805189329853 16.069640530417985 8 24.311944944692097 37.04757370298555 21.156659391622128 17.483821960700222 9 25.265999494805403 17.375059741987307 23.128008679170538 34.23093208403676 10-11 22.222065737084375 29.750782779042783 29.500593331067833 18.526558152805013 12-13 23.65636244684331 28.23533420564824 29.48687892996038 18.62142441754807 14-15 21.146674141611744 29.193900771921342 28.870430701307647 20.788994385159263 16-17 18.26155814371878 32.2437727496579 29.852321441876274 19.64234766474705 18-19 17.926185253769425 30.358333802788746 33.81387395213009 17.901606991311745 20-21 19.127811624029476 29.214085492451602 34.070954464297884 17.587148419221034 22-23 18.964780154803222 29.168190514179848 33.62933456058901 18.23769477042792 24-25 18.616855259278307 29.692625920607107 34.006821045018256 17.683697775096324 26-27 18.548608028669474 29.64344435272209 34.25802796527353 17.549919653334918 28-29 18.08143211779116 29.458285325899297 34.56694265640731 17.893339899902227 30-31 18.54443787710761 29.293921437589525 34.42828657475323 17.73335411054963 32-33 18.708853501998295 29.691626433525702 33.73632349580601 17.863196568669988 34-35 18.856388440129898 29.308646641818846 34.05161525978451 17.783349658266747 36-37 19.134072438214716 29.01908155398293 33.62053417084217 18.226311836960182 38-39 18.182925050803426 30.157587167687883 33.70176513801314 17.95772264349555 40-41 18.442692214733146 29.729157542573546 33.98828421012461 17.839866032568697 42-43 18.507846373820794 29.54915438903057 33.447920060083064 18.49507917706557 44-45 18.97714448747281 29.57382837558129 33.07843418749989 18.370592949446014 46-47 19.261134725223204 29.945727925449265 32.34830986964889 18.444827479678636 48-49 18.91013351311961 30.224629872265723 32.231052022323055 18.634184592291604 50-51 19.026800755247756 30.10401011842997 31.808133269608547 19.061055856713725 52-53 19.166365306578616 30.700748523941318 31.419469618360008 18.71341655112006 54-55 19.507825973181074 30.27460415822404 31.259915352645823 18.957654515949066 56-57 19.59419062555085 30.614520165078694 30.952709787509335 18.838579421861116 58-59 19.38706992583816 30.14167255757492 31.515783696752397 18.95547381983452 60-61 19.594508643734223 30.899328163872042 30.979377883743453 18.526785308650275 62-63 19.405923860995163 30.467232315008825 31.471397444587602 18.655446379408414 64-65 19.51200564073395 30.165705646003964 31.272499786473507 19.04978892678858 66-67 19.528678879776407 30.53483389455971 30.461462556539086 19.475024669124796 68-69 19.447584243016774 30.14512532642295 30.993870426671364 19.41342000388891 70-71 19.585922152783205 30.31381125711679 31.391075137701876 18.70919145239813 72-73 19.872083767958973 29.72153368636445 31.297861823574095 19.108520722102483 74-75 19.394248050548534 30.701929734336698 30.61551965079786 19.288302564316908 76-77 19.294708359153383 30.282645475146424 31.251737742216278 19.17090842348392 78-79 19.21497665746534 30.191783137040396 30.914593036673853 19.678647168820408 80-81 19.260816707039833 30.40017590948657 30.661087113358036 19.67792027011556 82-83 19.479811460048836 30.308137884036572 30.81247089556477 19.399579760349823 84-85 19.001095345983497 30.106522510473027 30.73283689157983 20.15954525196365 86-87 18.903682287114083 30.253296939938178 30.95098340308532 19.892037369862418 88-89 19.297434229296563 29.681954557927465 31.124167019515415 19.89644419326056 90-91 19.055552930400268 29.670393579435583 31.34423297808924 19.929820512074905 92-93 19.278135300224207 30.117781750529847 30.92796572630565 19.676117222940295 94-95 19.078183407446716 29.541390520877442 31.060472520503087 20.31995355117276 96-97 19.470981295079078 29.381154787627455 30.770167350254322 20.37769656703914 98-99 19.15904027389593 29.465210564764977 30.625524730956126 20.750224430382968 100-101 19.133879268707705 29.13760052270215 31.353132464374333 20.37538774421581 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1560.0 1 991.5 2 479.5 3 564.5 4 518.0 5 509.0 6 967.5 7 2046.0 8 3090.0 9 3490.5 10 3012.5 11 2340.0 12 2185.0 13 2152.0 14 2092.0 15 2079.5 16 2204.5 17 2386.0 18 2591.0 19 2909.0 20 3300.5 21 3756.0 22 4406.5 23 5208.0 24 6069.0 25 7741.5 26 11171.0 27 14540.0 28 15937.5 29 18093.0 30 22163.0 31 26342.0 32 32474.0 33 40349.0 34 47751.0 35 52291.0 36 56795.0 37 61316.0 38 59694.5 39 55804.5 40 52223.5 41 47540.5 42 42897.5 43 41451.0 44 41081.0 45 38888.0 46 35223.5 47 31814.0 48 30628.0 49 27726.0 50 22860.5 51 18827.0 52 15833.0 53 13616.5 54 11580.0 55 9670.5 56 7997.0 57 6427.5 58 5257.5 59 4215.5 60 3232.5 61 2481.5 62 1875.5 63 1368.5 64 955.5 65 654.0 66 467.5 67 340.5 68 222.0 69 147.5 70 114.0 71 84.5 72 60.5 73 51.0 74 45.0 75 42.5 76 26.0 77 12.0 78 10.5 79 9.5 80 7.5 81 5.0 82 3.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035618036537563395 2 0.0 3 0.0 4 0.0 5 9.086233810602909E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004179667552877338 14-15 4.543116905301454E-5 16-17 0.0 18-19 0.0 20-21 9.086233810602908E-5 22-23 6.81467535795218E-4 24-25 1.362935071590436E-4 26-27 0.002044402607385654 28-29 0.003816218200453221 30-31 1.362935071590436E-4 32-33 1.362935071590436E-4 34-35 0.0 36-37 4.543116905301454E-5 38-39 4.543116905301454E-5 40-41 0.0 42-43 0.008132179260489602 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.8172467621205816E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.1801818337110177E-4 84-85 4.543116905301454E-5 86-87 0.0 88-89 0.0 90-91 0.009267958486814967 92-93 0.0012266415644313926 94-95 0.0 96-97 0.0 98-99 1.8172467621205816E-4 100-101 0.011857535122836795 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1100566.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.15972360159823 #Duplication Level Percentage of deduplicated Percentage of total 1 79.68172249030546 34.390411187808375 2 11.720824410652614 10.117350838932646 3 3.5062193585120074 4.539823751998539 4 1.4551581637944502 2.512168965839107 5 0.7481077565100285 1.6144061997592287 6 0.4437690799167331 1.1491770499285052 7 0.2946745106459949 0.8902649302342149 8 0.20919435537002756 0.7223016445427924 9 0.16005980159521513 0.6217323116918523 >10 1.313822736686769 12.206380092463668 >50 0.24657219210978196 7.49249946750475 >100 0.20547099192182633 16.885356125694546 >500 0.012074068570772398 3.421064168038093 >1k 0.0016946061151961263 1.1693968939972008 >5k 6.354772931985473E-4 2.2676663715664582 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9958 0.9048071628598376 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8345 0.7582462114948126 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6501 0.590696060027295 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2332 0.21189097246325983 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2114 0.19208298275614547 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1829 0.1661872163959272 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1657 0.1505588942416902 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1332 0.12102863435723074 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1311 0.11912052525700412 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1198 0.10885308105102284 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 9.086233810602908E-5 0.0 24-25 0.0 0.0 0.0 3.1801818337110177E-4 0.0 26-27 0.0 0.0 0.0 6.360363667422035E-4 0.0 28-29 0.0 0.0 0.0 0.0017263844240145525 0.0 30-31 0.0 0.0 0.0 0.004997428595831599 0.0 32-33 0.0 0.0 0.0 0.01081261823461746 0.0 34-35 0.0 0.0 0.0 0.019262815678478165 0.0 36-37 0.0 0.0 0.0 0.03361906509923076 0.0 38-39 0.0 0.0 0.0 0.056561805471003104 0.0 40-41 0.0 0.0 0.0 0.08327533287417566 0.0 42-43 0.0 0.0 0.0 0.11112463950367356 0.0 44-45 0.0 0.0 0.0 0.1511949306084324 0.0 46-47 0.0 0.0 0.0 0.2043493984004594 0.0 48-49 0.0 0.0 0.0 0.2609112038714625 0.0 50-51 0.0 0.0 0.0 0.3232427678121984 0.0 52-53 0.0 0.0 0.0 0.400066874680846 0.0 54-55 0.0 0.0 0.0 0.490384038758239 0.0 56-57 0.0 0.0 0.0 0.5991462574711558 0.0 58-59 0.0 0.0 0.0 0.7183576450662659 0.0 60-61 0.0 0.0 0.0 0.8581493522423916 0.0 62-63 0.0 0.0 0.0 1.0019844334642356 0.0 64-65 0.0 0.0 0.0 1.1657183667313 0.0 66-67 0.0 0.0 0.0 1.3563475520777493 0.0 68-69 0.0 0.0 0.0 1.5790511427756264 0.0 70-71 0.0 0.0 0.0 1.8477310765551542 0.0 72-73 0.0 0.0 0.0 2.112594792134229 0.0 74-75 0.0 0.0 0.0 2.4140760299700332 0.0 76-77 0.0 0.0 0.0 2.749539782257493 0.0 78-79 0.0 0.0 0.0 3.1357501503771696 0.0 80-81 0.0 0.0 0.0 3.555852170610395 0.0 82-83 0.0 0.0 0.0 3.9830414532158906 0.0 84-85 0.0 0.0 0.0 4.417636016377028 0.0 86-87 0.0 0.0 0.0 4.902568314848905 0.0 88-89 0.0 0.0 0.0 5.430296774568722 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATACCG 50 2.827852E-5 47.49734 5 GGTATCA 6880 0.0 31.626053 1 ACTCCCG 325 0.0 29.230463 6 GTATCAA 9830 0.0 28.272896 1 CTCCCGC 355 0.0 28.103405 7 GGTACTC 375 0.0 26.59851 3 ACCACCG 95 0.0012269218 24.9986 5 GTACATG 4720 0.0 24.659943 1 CATTCCG 255 1.8189894E-11 24.219833 9 TCGCTGA 50 0.0016542266 23.74975 26-27 GAGTACT 7600 0.0 23.659763 12-13 GACATAT 550 0.0 23.322174 1 GACAATG 695 0.0 23.241388 7 GTGGTAC 430 0.0 23.201645 1 ATCAACG 12105 0.0 22.836391 3 TCAACGC 12180 0.0 22.695774 4 CAACGCA 12310 0.0 22.533264 5 TATCAAC 12455 0.0 22.2328 2 AACGCAG 12515 0.0 22.203121 6 AGTACTT 8075 0.0 22.091515 12-13 >>END_MODULE