FastQCFastQC Report
Thu 26 May 2016
SRR937190_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937190_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074167
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99570.9269508372534253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72470.6746623197324066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58170.5415359064279577No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17720.16496503802481366No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15550.1447633375443483No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15140.14094642639366134No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14980.1394569000909542No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11950.11124899573343809No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11410.10622184446180155No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT11160.10389445961382168No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT10970.10212564712935698No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATG250.001617773237.9921358-59
GGTATCA42900.037.1328661
GTATCAA71550.035.423261
ACCGTCA550.0025792234.5463528
CATTCCG2700.031.667499
ATCAACG87750.028.5992763
TCAACGC87950.028.5382374
CAACGCA88800.028.2637465
GTACATG52650.028.1791021
AACGCAG90300.027.7222066
TATCAAC91450.027.443452
CTCCCGC2450.027.143567
TACATGG56200.025.6268272
ACATGGG53050.025.6241153
ACGCAGA99500.024.8725057
CGCAGAG99750.024.762558
GGTACTC2701.8189894E-1224.645213
CATGGGG15300.024.5450944
ATGGGTA6050.024.3565125
GCAGAGT104550.023.2622039
Produced by FastQC (version 0.11.5)