##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937190_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1074167 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.323378953179535 33.0 31.0 34.0 31.0 34.0 2 32.48098945508473 34.0 31.0 34.0 31.0 34.0 3 32.50708316304634 34.0 31.0 34.0 31.0 34.0 4 36.0902997392398 37.0 37.0 37.0 35.0 37.0 5 36.11560492921492 37.0 37.0 37.0 35.0 37.0 6 36.192025076175305 37.0 37.0 37.0 35.0 37.0 7 36.16055231635304 37.0 37.0 37.0 35.0 37.0 8 36.1650264809848 37.0 37.0 37.0 35.0 37.0 9 37.8910085675691 39.0 38.0 39.0 35.0 39.0 10-11 37.81718810948391 39.0 38.0 39.0 35.0 39.0 12-13 37.74520069970498 39.0 38.0 39.0 35.0 39.0 14-15 39.056449323056846 40.0 38.5 41.0 36.0 41.0 16-17 39.02867477775802 40.0 38.5 41.0 36.0 41.0 18-19 39.070718519559804 40.0 38.5 41.0 36.0 41.0 20-21 39.05069276937385 40.0 39.0 41.0 36.0 41.0 22-23 38.913854642713844 40.0 38.5 41.0 36.0 41.0 24-25 38.80035553130938 40.0 38.0 41.0 35.0 41.0 26-27 38.65664696457813 40.0 38.0 41.0 35.0 41.0 28-29 38.70378442085821 40.0 38.0 41.0 35.0 41.0 30-31 38.53054925351458 40.0 38.0 41.0 35.0 41.0 32-33 38.318079963357654 40.0 38.0 41.0 35.0 41.0 34-35 38.20631568461887 40.0 38.0 41.0 35.0 41.0 36-37 38.185053162124696 40.0 38.0 41.0 35.0 41.0 38-39 38.100703149510274 40.0 38.0 41.0 35.0 41.0 40-41 37.968144152631766 40.0 38.0 41.0 34.5 41.0 42-43 37.73184104520061 40.0 38.0 41.0 34.0 41.0 44-45 37.44209513045923 40.0 38.0 41.0 33.0 41.0 46-47 37.03757376646276 40.0 37.5 41.0 33.0 41.0 48-49 36.69215634068073 40.0 37.0 41.0 31.5 41.0 50-51 35.83694667588932 39.0 36.0 40.0 30.5 40.5 52-53 35.932941991329095 39.0 36.5 40.0 31.0 40.5 54-55 36.300095795160345 40.0 37.0 41.0 31.0 41.0 56-57 36.17181499710939 40.0 36.5 41.0 30.5 41.0 58-59 36.00762963300865 39.5 36.0 41.0 30.0 41.0 60-61 35.788905263334286 39.0 36.0 41.0 29.0 41.0 62-63 35.5218699699395 39.0 35.5 40.5 28.5 41.0 64-65 35.22302072210373 39.0 35.0 40.0 28.0 41.0 66-67 34.88817055448548 38.0 35.0 40.0 27.5 41.0 68-69 34.43723368898877 37.5 35.0 40.0 26.0 41.0 70-71 34.037127374048914 37.0 34.0 39.5 26.0 41.0 72-73 33.64268777573692 36.5 34.0 39.0 25.5 40.5 74-75 33.17374951939503 36.0 34.0 39.0 24.5 40.0 76-77 32.72732405668765 35.5 33.5 38.0 22.5 39.5 78-79 32.247742669435944 35.0 33.0 37.0 20.5 39.0 80-81 31.76086772354764 35.0 33.0 37.0 19.0 39.0 82-83 31.28003187586288 35.0 32.0 36.0 15.5 38.0 84-85 30.83541106736662 35.0 32.0 36.0 8.5 37.0 86-87 30.45450335003775 34.5 31.0 35.0 4.5 37.0 88-89 30.069464990080686 34.0 31.0 35.0 2.0 36.0 90-91 29.69065145363803 34.0 31.0 35.0 2.0 36.0 92-93 29.319056999516835 34.0 30.5 35.0 2.0 36.0 94-95 28.880450618944728 34.0 30.0 35.0 2.0 35.0 96-97 28.387664581019525 34.0 29.0 35.0 2.0 35.0 98-99 27.800267090685153 34.0 29.0 35.0 2.0 35.0 100-101 25.9238693797147 32.5 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04728803314903729 1101 2 -0.037140582139358 1101 3 -0.016766257779735838 1101 4 -0.11307154139399245 1101 5 -0.13840748292471972 1101 6 -0.05769411496188326 1101 7 -0.013097835093212495 1101 8 -0.014639008910371842 1101 9 0.017638205070589663 1101 10-11 0.06624317105884359 1101 12-13 -0.007843525935818718 1101 14-15 0.05283071024812358 1101 16-17 0.12627683207436746 1101 18-19 -0.06464529509131722 1101 20-21 0.032015606229180094 1101 22-23 0.07082213566505402 1101 24-25 0.01534461436813217 1101 26-27 0.061251104251539346 1101 28-29 -0.14588215288608808 1101 30-31 -0.1348342236830078 1101 32-33 0.0021056388029023765 1101 34-35 -0.02667825024142445 1101 36-37 -0.0946158240903685 1101 38-39 -0.06125486353940346 1101 40-41 -0.16596669323536162 1101 42-43 -0.14698799580367705 1101 44-45 0.08279831806722626 1101 46-47 0.18134346729657125 1101 48-49 0.3458501947512431 1101 50-51 0.29252726775525417 1101 52-53 0.2460618789538742 1101 54-55 0.37703715613491084 1101 56-57 0.271343229017063 1101 58-59 0.41746370858808746 1101 60-61 0.2824633357739188 1101 62-63 0.34059664855728755 1101 64-65 0.4786390254070483 1101 66-67 0.47409251425568044 1101 68-69 0.33808919964533146 1101 70-71 0.3529481003752011 1101 72-73 0.15353756213998082 1101 74-75 0.10871443730793118 1101 76-77 0.1850280466635681 1101 78-79 -0.03544417544074463 1101 80-81 0.14070804653298907 1101 82-83 0.12257827200740223 1101 84-85 0.2773626250120671 1101 86-87 0.3473093945795469 1101 88-89 0.24186859911513992 1101 90-91 0.2912330288600735 1101 92-93 0.38249000099903085 1101 94-95 0.48276485387367885 1101 96-97 0.3715785876166393 1101 98-99 0.4863266363291672 1101 100-101 1.0516523430382527 1102 1 -0.0014264726592188026 1102 2 0.018164461112597508 1102 3 0.03515390121840767 1102 4 0.06742554150302738 1102 5 0.025765933327001278 1102 6 -0.008926136845495591 1102 7 0.06051835156338825 1102 8 -0.006528821489176551 1102 9 0.10629462803984069 1102 10-11 0.09599477208178797 1102 12-13 0.11555752882784276 1102 14-15 0.22045193564508736 1102 16-17 0.26418148068039926 1102 18-19 0.23990168130828948 1102 20-21 0.16958485329129758 1102 22-23 0.02041158043006419 1102 24-25 0.174164072169269 1102 26-27 0.08560184997086395 1102 28-29 0.18213402712184035 1102 30-31 0.17843081833419205 1102 32-33 0.23181532947363337 1102 34-35 0.12275590064059116 1102 36-37 0.16290013864860242 1102 38-39 0.13925767751018014 1102 40-41 0.32803941404089443 1102 42-43 0.2659405761337226 1102 44-45 0.052003113656304834 1102 46-47 0.278827777627896 1102 48-49 0.36392269847330283 1102 50-51 0.34887413350202934 1102 52-53 0.2817675994201281 1102 54-55 0.2960575699194905 1102 56-57 0.21720941982908215 1102 58-59 0.30486258353851525 1102 60-61 0.29663687075184697 1102 62-63 0.268450886014449 1102 64-65 0.2610631534223984 1102 66-67 0.32975290505486754 1102 68-69 0.3439750515177593 1102 70-71 0.294616132393287 1102 72-73 0.282759169360439 1102 74-75 0.3068275216486569 1102 76-77 0.3538276296971148 1102 78-79 0.46954469991334946 1102 80-81 0.5447238418679952 1102 82-83 0.36933526557481855 1102 84-85 0.4486049998212138 1102 86-87 0.22991707331592082 1102 88-89 0.27978381435388755 1102 90-91 0.4120701911136848 1102 92-93 0.3615823153765376 1102 94-95 0.4046815935569654 1102 96-97 0.5219910424709973 1102 98-99 0.5896694915045622 1102 100-101 0.756494235344082 1103 1 0.055599264161038775 1103 2 0.016777301848726722 1103 3 -0.012635638009484751 1103 4 0.036468712943452886 1103 5 0.07284978554783805 1103 6 0.009992501593686143 1103 7 -0.08360438262783987 1103 8 0.026908289345193737 1103 9 -0.0481574267546776 1103 10-11 -0.006210499815267667 1103 12-13 0.04046758832694053 1103 14-15 0.03445152053507883 1103 16-17 -0.11167641332817624 1103 18-19 -0.06032144031894404 1103 20-21 -0.07756991355524434 1103 22-23 -0.112513561399183 1103 24-25 -0.02023367239970497 1103 26-27 0.036153600351383375 1103 28-29 -0.07056245828012209 1103 30-31 -0.004911163124226903 1103 32-33 0.005298448333462602 1103 34-35 0.06163960065443064 1103 36-37 0.1430803655754005 1103 38-39 0.0021208987638132726 1103 40-41 0.13080681406857053 1103 42-43 0.07120209543634104 1103 44-45 0.2731944793682217 1103 46-47 0.17851644512478515 1103 48-49 0.035300863687091066 1103 50-51 0.09878938187618047 1103 52-53 0.15237954075124804 1103 54-55 -0.029437102835451867 1103 56-57 0.10595474984153697 1103 58-59 0.17843910214929082 1103 60-61 0.09249441883542175 1103 62-63 -0.1308849379789052 1103 64-65 -0.02782400584147382 1103 66-67 0.1629617053869623 1103 68-69 0.19161411336914824 1103 70-71 0.23146060451173156 1103 72-73 0.2271240510540835 1103 74-75 0.31958523577623765 1103 76-77 0.32895562194839556 1103 78-79 0.23759333696883544 1103 80-81 0.2328793697495506 1103 82-83 0.18249944158146292 1103 84-85 0.36222233758238787 1103 86-87 0.3671334148130825 1103 88-89 0.30652727637132315 1103 90-91 0.3550029432930124 1103 92-93 0.4459378570950925 1103 94-95 0.4813005225731537 1103 96-97 0.34931046961919066 1103 98-99 0.3826835360597016 1103 100-101 0.5847528488766685 1104 1 0.04855473348845862 1104 2 -0.006268707020453235 1104 3 0.0595650271789836 1104 4 -0.06955031883776996 1104 5 0.0389390316675744 1104 6 -0.0025675501431905445 1104 7 0.03796804900557049 1104 8 -0.043657120854362574 1104 9 -0.03016962187663097 1104 10-11 0.10499145879522587 1104 12-13 0.04368728529663457 1104 14-15 0.0813565464035193 1104 16-17 0.047370611801170526 1104 18-19 0.09211065502474014 1104 20-21 0.015937569815037023 1104 22-23 0.11669236986467268 1104 24-25 0.11118262471781293 1104 26-27 -0.005919570380321204 1104 28-29 -0.01017812716408173 1104 30-31 0.026629856831426935 1104 32-33 0.06794395831128952 1104 34-35 0.17844022888798605 1104 36-37 0.08367303002477655 1104 38-39 0.16962367038244253 1104 40-41 0.23043957460071596 1104 42-43 0.16026344059524433 1104 44-45 0.11476413938300567 1104 46-47 0.2555582041787403 1104 48-49 0.41722067152152675 1104 50-51 0.37227654004321664 1104 52-53 0.2880664402338837 1104 54-55 0.35745314106699055 1104 56-57 0.30114137216229864 1104 58-59 0.38181354782556554 1104 60-61 0.5283360670246324 1104 62-63 0.3502911521910832 1104 64-65 0.377890147890966 1104 66-67 0.5017967016914682 1104 68-69 0.5744416263033685 1104 70-71 0.770953860239743 1104 72-73 0.7051266378981396 1104 74-75 0.5893884508538392 1104 76-77 0.6556139737591877 1104 78-79 0.5699450553723864 1104 80-81 0.6399054229646275 1104 82-83 0.569442808174216 1104 84-85 0.6169294698809793 1104 86-87 0.5710945197650403 1104 88-89 0.5346615151000726 1104 90-91 0.5286425404844373 1104 92-93 0.640965111344908 1104 94-95 0.7726600015088536 1104 96-97 0.6408778199517862 1104 98-99 0.8639889684322064 1104 100-101 1.2324085214561222 1105 1 0.14788342653954345 1105 2 0.1583982280922953 1105 3 0.14862508240217664 1105 4 0.09189181185712414 1105 5 0.08949644077939922 1105 6 0.15267117512141937 1105 7 0.175026263455635 1105 8 0.20586197716680488 1105 9 0.20910672418871457 1105 10-11 0.21718715598805005 1105 12-13 0.2650787947305915 1105 14-15 0.3085367120730993 1105 16-17 0.22720580508234178 1105 18-19 0.29062624407213633 1105 20-21 0.23276885145332926 1105 22-23 0.26228918302619775 1105 24-25 0.3750879399502054 1105 26-27 0.4113894494079844 1105 28-29 0.30569969151976295 1105 30-31 0.1943898660352943 1105 32-33 0.04124613272454525 1105 34-35 0.22610072129479875 1105 36-37 0.19682679210484366 1105 38-39 0.42048710802625067 1105 40-41 0.07674163396564637 1105 42-43 0.0891980931493137 1105 44-45 -0.10847504607488645 1105 46-47 0.09536607348384507 1105 48-49 0.24045237883860437 1105 50-51 0.08085770663318215 1105 52-53 0.08821859163747092 1105 54-55 0.2422610103720899 1105 56-57 0.35275434961283025 1105 58-59 0.26295453954208625 1105 60-61 0.41215279505040314 1105 62-63 0.39255702885928656 1105 64-65 0.3884038386410822 1105 66-67 0.5431732548437935 1105 68-69 0.5148831242325045 1105 70-71 0.462915721721572 1105 72-73 0.599322450139276 1105 74-75 0.510631547954624 1105 76-77 0.45783498158247227 1105 78-79 0.37313793388135963 1105 80-81 0.4352292839862564 1105 82-83 0.5948250562183972 1105 84-85 0.6969979236315567 1105 86-87 0.5761914479748889 1105 88-89 0.6162385399505119 1105 90-91 0.7530618340877524 1105 92-93 0.8643926884272837 1105 94-95 0.7351855997601184 1105 96-97 0.8660385999793938 1105 98-99 0.9283567779121888 1105 100-101 0.983703677921838 1106 1 0.04820388176565871 1106 2 0.023306883378310772 1106 3 0.053994953621106845 1106 4 0.08232008379482636 1106 5 -0.02610403840598252 1106 6 0.034920351521535054 1106 7 0.03764381362035607 1106 8 0.10122286365976407 1106 9 0.00620765261039935 1106 10-11 -0.21892695003171525 1106 12-13 -0.3355785877192332 1106 14-15 -0.20559176950821012 1106 16-17 -0.26820599485775887 1106 18-19 0.01383079633329487 1106 20-21 -0.0866067100920418 1106 22-23 -0.03145295533857961 1106 24-25 -0.07151433618037117 1106 26-27 -0.1747590980613154 1106 28-29 0.09803422281655827 1106 30-31 -0.015932086645143784 1106 32-33 0.03421258974760377 1106 34-35 -0.1260046994898687 1106 36-37 -0.013719778724741616 1106 38-39 0.0425790517219653 1106 40-41 0.05194678520853557 1106 42-43 -0.05589025665601355 1106 44-45 0.02799245916620663 1106 46-47 0.17065208726042158 1106 48-49 0.17823015661637953 1106 50-51 0.20919702978382304 1106 52-53 0.06520421607591942 1106 54-55 -0.0016593250576804053 1106 56-57 -0.0949290885423082 1106 58-59 0.0381252493354296 1106 60-61 -0.03176604292504237 1106 62-63 -0.03503356712753458 1106 64-65 0.08723732171985432 1106 66-67 0.1904508828761422 1106 68-69 -0.06273292847789236 1106 70-71 0.12500317305429576 1106 72-73 0.19541409434412316 1106 74-75 0.23812780431880753 1106 76-77 0.23516052815330823 1106 78-79 0.32873691561241714 1106 80-81 0.361559023429205 1106 82-83 0.24997779655981844 1106 84-85 0.10711771997776864 1106 86-87 0.13338521856488583 1106 88-89 0.385170855014902 1106 90-91 0.48862487691494394 1106 92-93 0.8031348267920642 1106 94-95 0.6830681993408341 1106 96-97 0.7802808880896102 1106 98-99 0.9226005634767311 1106 100-101 1.007119371243192 1107 1 0.018536459241090597 1107 2 -0.031152678224110275 1107 3 -0.05072915824586488 1107 4 -0.006115290354834713 1107 5 -0.06060895148232959 1107 6 0.011828892715790573 1107 7 -0.0429306383826642 1107 8 0.05976405791524542 1107 9 -0.004676701131096195 1107 10-11 0.08230131905369831 1107 12-13 0.08518860804795736 1107 14-15 0.18735985558627277 1107 16-17 0.19165795072046876 1107 18-19 0.023959292176073177 1107 20-21 0.0381665325383409 1107 22-23 0.1173943613658821 1107 24-25 0.10242746276142611 1107 26-27 0.16276924660989067 1107 28-29 0.19718388268050546 1107 30-31 0.2880172708756348 1107 32-33 0.2352141014634057 1107 34-35 0.20135735644361574 1107 36-37 0.15912602626391958 1107 38-39 0.2809577365292242 1107 40-41 0.35599062496665823 1107 42-43 0.5476244825730063 1107 44-45 0.5582078786673392 1107 46-47 0.6075379527177205 1107 48-49 0.6925914566919644 1107 50-51 0.5303421545003815 1107 52-53 0.49099786686957003 1107 54-55 0.4859823982983329 1107 56-57 0.6357398456980619 1107 58-59 0.7337601699703598 1107 60-61 0.9017373584355042 1107 62-63 0.7736021093652852 1107 64-65 0.838163247645781 1107 66-67 0.9847063628459054 1107 68-69 0.9557100042507543 1107 70-71 0.9053130747927725 1107 72-73 0.8194881684182036 1107 74-75 0.8380144256339932 1107 76-77 0.7867478297406052 1107 78-79 0.730305320752251 1107 80-81 0.8654327951601211 1107 82-83 0.9753935184041111 1107 84-85 1.040035331466811 1107 86-87 0.9063632986858252 1107 88-89 0.8770642790511829 1107 90-91 0.9878501225687621 1107 92-93 1.0893026272455764 1107 94-95 1.2513977533846692 1107 96-97 1.3631418102362467 1107 98-99 1.4756617879665264 1107 100-101 1.6275842238509028 1108 1 -0.07772184081648703 1108 2 0.02825972795452003 1108 3 -0.030427895713351916 1108 4 -0.04258993658022092 1108 5 0.02794900992069671 1108 6 0.029679572145475674 1108 7 0.05566902011597108 1108 8 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-2.621785248359746 2316 72-73 -2.5053584276430385 2316 74-75 -2.3634581767180904 2316 76-77 -2.6363169036402674 2316 78-79 -2.6680468838752063 2316 80-81 -2.6693517601854033 2316 82-83 -2.7418948759700967 2316 84-85 -2.507605096492135 2316 86-87 -2.570424569354717 2316 88-89 -2.4457782245614688 2316 90-91 -2.686678849678298 2316 92-93 -2.7874919064303434 2316 94-95 -3.0760924588214884 2316 96-97 -2.8932757503172795 2316 98-99 -3.0581669404188965 2316 100-101 -3.5218964225396583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3628.0 3 470.0 4 351.0 5 395.0 6 410.0 7 404.0 8 527.0 9 646.0 10 814.0 11 1260.0 12 2275.0 13 3550.0 14 3812.0 15 5804.0 16 8916.0 17 14232.0 18 11271.0 19 5352.0 20 4176.0 21 3832.0 22 3849.0 23 4129.0 24 4775.0 25 5426.0 26 5950.0 27 7140.0 28 8751.0 29 10472.0 30 13233.0 31 17241.0 32 23565.0 33 34089.0 34 51578.0 35 85390.0 36 141700.0 37 256602.0 38 282531.0 39 45603.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.45963415180906 22.959010207284088 16.12653744414389 21.45481819676296 2 13.883090160169392 22.594845002782147 41.86343962745398 21.65862520959448 3 16.535374998132852 28.371697014055897 32.490626913827356 22.602301073983895 4 10.443346718289465 19.433891978642574 42.01958168355424 28.103179619513718 5 10.622128570935093 39.985136955671344 36.93643901250382 12.456295460889748 6 25.800014181009388 41.74161454523742 19.372098490842056 13.086272782911138 7 24.330957667120657 35.466986324371724 23.877590999832083 16.324465008675535 8 23.833321982888506 35.959142130270145 21.906203033647383 18.30133285319397 9 25.084888376449165 16.996798495516774 23.508110104888452 34.410203023145606 10-11 21.70893894467345 28.96479881169865 30.248548678742505 19.077713564885396 12-13 22.993914549944446 27.76511077049093 29.86647087744111 19.374503802123517 14-15 20.800956031238936 28.653183528984826 29.048031520670865 21.497828919105373 16-17 18.065989814566905 31.769539416543914 29.98433656148809 20.18013420740109 18-19 17.711595383152968 30.19668148960762 33.59928805106778 18.49243507617163 20-21 18.836085391858184 29.327659447449083 33.54823393772282 18.28802122296991 22-23 18.663981452245174 29.252357763467618 33.06628882130249 19.01737196298471 24-25 18.204152671926604 29.686772767083063 33.47227805609213 18.636796504898197 26-27 18.085730022203638 29.877257381742652 33.56942286858346 18.467589727470255 28-29 17.7774958440691 29.49087747513438 34.02895811605262 18.7026685647439 30-31 18.21201259140806 29.426725207633215 33.88045999317064 18.480802207788088 32-33 18.036364823703373 29.753665169346448 33.416688823274235 18.793281183675944 34-35 18.457993081844624 29.6539289571219 33.25276924476415 18.63530871626933 36-37 18.619310770040123 29.176260551329868 33.09149825969225 19.112930418937758 38-39 17.723292943067168 30.38988996598338 33.09051293554937 18.79630415540008 40-41 17.93237600845801 29.957901148146266 33.58893295010491 18.52078989329081 42-43 17.94295975001166 29.650156242712562 33.00531691618861 19.40156709108717 44-45 18.327298643232513 29.796272655740946 32.65752266915051 19.21890603187604 46-47 18.55958766733523 30.004804328560102 32.19403890106815 19.24156910303652 48-49 18.19062594897118 30.260676406833014 32.17697429153981 19.371723352656 50-51 18.30859635758392 30.207115124549432 31.905870586517526 19.578417931349122 52-53 18.349810407501398 30.874486359931343 31.524331153964415 19.251372078602852 54-55 18.722947913056416 30.293812660901466 31.525421673202626 19.457817752839492 56-57 18.753246922826534 30.730649738987044 30.98961077964388 19.526492558542543 58-59 18.64148155886018 30.223108691921414 31.58982547511211 19.545584274106297 60-61 18.880157509910823 30.942424662127344 31.165193741946943 19.012224086014886 62-63 18.59655913777906 30.640882741123853 31.62328668099971 19.139271440097378 64-65 18.657528331125228 30.272625144972448 31.529216232437175 19.54063029146515 66-67 18.674233592300016 30.566167075479616 30.80385372268492 19.955745609535448 68-69 18.660894397124558 30.138036452217897 31.383078433914385 19.817990716743157 70-71 18.736140304077356 30.31656186735079 31.636412985943384 19.31088484262847 72-73 18.956905693955157 29.82431349724895 31.575535589201753 19.64324521959414 74-75 18.49034411225902 30.604276498658695 31.112488480466226 19.79289090861605 76-77 18.369710251654368 30.3312562290974 31.518101867808117 19.780931651440113 78-79 18.354563503260195 30.130813539987226 31.29670454720345 20.217918409549124 80-81 18.539486454331726 30.272396442294554 30.964638877786538 20.22347822558718 82-83 18.731162627585817 30.311830068072194 31.136092627058847 19.820914677283138 84-85 18.50395389807999 29.8975595789348 31.066755080285724 20.53173144269949 86-87 18.302339646784354 30.06054179379604 31.346034845922233 20.291083713497375 88-89 18.79651598750993 29.690956639172107 31.28285264481278 20.229674728505184 90-91 18.39254726629411 29.67144789853224 31.643432059234613 20.292572775939043 92-93 18.735916596405094 29.915714093849566 31.422871447438126 19.925497862307214 94-95 18.5241126787886 29.26523200549143 31.56971169988137 20.6409436158386 96-97 18.798501215955007 29.13684681764872 31.281637563799748 20.783014402596525 98-99 18.602978759919562 29.28406602221385 31.10274557793688 21.01020963992971 100-101 18.53981991392053 28.950492648738884 31.745930343711677 20.763757093628907 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2322.0 1 1409.5 2 609.0 3 737.0 4 603.5 5 581.5 6 1110.5 7 2348.5 8 3607.5 9 3889.5 10 3091.0 11 2277.0 12 2213.0 13 2242.5 14 2183.0 15 2184.5 16 2208.5 17 2312.0 18 2526.5 19 2762.5 20 3094.0 21 3654.5 22 4300.5 23 4987.5 24 5701.0 25 7167.0 26 10383.5 27 13751.0 28 15008.5 29 16815.0 30 20910.5 31 25118.5 32 30964.5 33 38796.0 34 46555.0 35 50954.5 36 55807.5 37 61227.0 38 58796.0 39 53818.5 40 49749.0 41 45580.0 42 41722.5 43 40467.0 44 40241.5 45 38169.0 46 34676.0 47 30739.0 48 29830.5 49 27499.5 50 22421.5 51 18145.5 52 15393.5 53 13419.0 54 11474.0 55 9804.5 56 8337.5 57 6856.5 58 5433.5 59 4235.5 60 3273.5 61 2569.0 62 1925.0 63 1300.0 64 855.0 65 603.0 66 425.0 67 284.5 68 205.0 69 149.0 70 100.0 71 72.5 72 59.0 73 58.0 74 45.5 75 31.5 76 30.0 77 26.0 78 16.0 79 11.0 80 11.0 81 7.0 82 6.0 83 5.5 84 2.5 85 2.5 86 2.5 87 2.5 88 2.5 89 1.5 90 1.0 91 1.5 92 1.0 93 1.5 94 2.0 95 2.0 96 3.0 97 2.0 98 2.5 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33132650695841526 2 0.28403404684746414 3 0.2806826126663731 4 0.28524428696841364 5 0.2843133330292217 6 0.2151434553472598 7 0.2035996265012796 8 0.21002320868170407 9 0.2021101001985725 10-11 0.2220325144972802 12-13 0.210954162620896 14-15 0.20811475310636057 16-17 0.2089060639546737 18-19 0.20294795874384522 20-21 0.1974553305026127 22-23 0.1976880689874107 24-25 0.18972841280731953 26-27 0.1904266282617135 28-29 0.20527534359182512 30-31 0.2164467908621285 32-33 0.20965082710602728 34-35 0.2187276280131488 36-37 0.1942435394124005 38-39 0.1902869851708347 40-41 0.19061281904955188 42-43 0.19708294892693592 44-45 0.2020635525016129 46-47 0.20639248831885543 48-49 0.2124902366205627 50-51 0.2163071477712497 52-53 0.2352055127368463 54-55 0.21193166425704757 56-57 0.18600459705055172 58-59 0.1860976924444709 60-61 0.18437542765696582 62-63 0.19103174832218825 64-65 0.18437542765696582 66-67 0.18218768589986473 68-69 0.17818458396133935 70-71 0.18977496050427914 72-73 0.18898364965596598 74-75 0.19317294238232974 76-77 0.19377806244280452 78-79 0.192567822321855 80-81 0.19284710850361256 82-83 0.18586495395967295 84-85 0.18004649183972324 86-87 0.17990684874884447 88-89 0.18200149511202635 90-91 0.1883785295954912 92-93 0.18460816614176379 94-95 0.18162911353634956 96-97 0.1828859013542587 98-99 0.1901938897769155 100-101 0.17706743923430898 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1074167.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.851998124216564 #Duplication Level Percentage of deduplicated Percentage of total 1 78.56707273785769 32.88188980850007 2 12.320655566244929 10.312881072952024 3 3.694106007292667 4.638171530636096 4 1.5833560926829768 2.650664648837393 5 0.7758701024496968 1.623585703618021 6 0.4716814544018276 1.1844486806911818 7 0.311555930086345 0.9127466741093583 8 0.22633112271093306 0.757792777851984 9 0.1755690176918215 0.6613122779197794 >10 1.3833315116782017 12.37850870178278 >50 0.2543531474073868 7.492080534086982 >100 0.21899769290872262 17.281556597870267 >500 0.014451624391543445 3.9749385337226584 >1k 0.0020009941465214003 1.1073029765274505 >5k 6.669980488404667E-4 2.1421194808939865 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9957 0.9269508372534253 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7247 0.6746623197324066 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5817 0.5415359064279577 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1772 0.16496503802481366 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1555 0.1447633375443483 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1514 0.14094642639366134 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1498 0.1394569000909542 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1195 0.11124899573343809 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1141 0.10622184446180155 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1116 0.10389445961382168 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1097 0.10212564712935698 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 9.309539391919506E-5 0.0 2 0.0 9.309539391919506E-5 0.0 9.309539391919506E-5 0.0 3 0.0 9.309539391919506E-5 0.0 9.309539391919506E-5 0.0 4 0.0 9.309539391919506E-5 0.0 1.8619078783839013E-4 0.0 5 0.0 9.309539391919506E-5 0.0 1.8619078783839013E-4 0.0 6 0.0 9.309539391919506E-5 0.0 1.8619078783839013E-4 0.0 7 0.0 9.309539391919506E-5 0.0 1.8619078783839013E-4 0.0 8 0.0 9.309539391919506E-5 0.0 1.8619078783839013E-4 0.0 9 0.0 9.309539391919506E-5 0.0 3.7238157567678025E-4 0.0 10-11 0.0 9.309539391919506E-5 0.0 3.7238157567678025E-4 0.0 12-13 0.0 9.309539391919506E-5 0.0 3.7238157567678025E-4 0.0 14-15 0.0 9.309539391919506E-5 0.0 4.189292726363778E-4 0.0 16-17 0.0 9.309539391919506E-5 0.0 4.654769695959753E-4 0.0 18-19 0.0 9.309539391919506E-5 0.0 4.654769695959753E-4 0.0 20-21 0.0 9.309539391919506E-5 0.0 6.051200604747679E-4 0.0 22-23 0.0 9.309539391919506E-5 0.0 6.516677574343654E-4 0.0 24-25 0.0 9.309539391919506E-5 0.0 9.77501636151548E-4 0.0 26-27 0.0 9.309539391919506E-5 0.0 0.0010705970300707432 0.0 28-29 0.0 9.309539391919506E-5 0.0 0.0021411940601414863 0.0 30-31 0.0 9.309539391919506E-5 0.0 0.0049806035746769355 0.0 32-33 0.0 9.309539391919506E-5 0.0 0.01093870878550542 0.0 34-35 0.0 9.309539391919506E-5 0.0 0.01903800805647539 0.0 36-37 0.0 9.309539391919506E-5 0.0 0.03290922175043545 0.0 38-39 0.0 9.309539391919506E-5 0.0 0.05418151926097152 0.0 40-41 0.0 9.309539391919506E-5 0.0 0.07927072792219458 0.0 42-43 0.0 9.309539391919506E-5 0.0 0.1075251799766703 0.0 44-45 0.0 9.309539391919506E-5 0.0 0.14532190990786348 0.0 46-47 0.0 9.309539391919506E-5 0.0 0.19224198844313778 0.0 48-49 0.0 9.309539391919506E-5 0.0 0.24586493534059414 0.0 50-51 0.0 9.309539391919506E-5 0.0 0.30544598744887896 0.0 52-53 0.0 9.309539391919506E-5 0.0 0.37280050494941663 0.0 54-55 0.0 9.309539391919506E-5 0.0 0.45546921474966184 0.0 56-57 0.0 9.309539391919506E-5 0.0 0.5665320196952615 0.0 58-59 0.0 9.309539391919506E-5 0.0 0.6881611518506898 0.0 60-61 0.0 1.8619078783839013E-4 0.0 0.8273387657598865 0.0 62-63 0.0 1.8619078783839013E-4 0.0 0.9765706822123561 0.0 64-65 0.0 1.8619078783839013E-4 0.0 1.1445147728425842 0.0 66-67 0.0 1.8619078783839013E-4 0.0 1.340340933951611 0.0 68-69 0.0 1.8619078783839013E-4 0.0 1.56944869838675 0.0 70-71 0.0 1.8619078783839013E-4 0.0 1.8389598637828195 0.0 72-73 0.0 1.8619078783839013E-4 0.0 2.1143825866927584 0.0 74-75 0.0 1.8619078783839013E-4 0.0 2.416849521536223 0.0 76-77 0.0 1.8619078783839013E-4 0.0 2.766003796430164 0.0 78-79 0.0 1.8619078783839013E-4 0.0 3.16663982416142 0.0 80-81 0.0 1.8619078783839013E-4 0.0 3.6053518680056267 0.0 82-83 0.0 1.8619078783839013E-4 0.0 4.04671713057653 0.0 84-85 0.0 1.8619078783839013E-4 0.0 4.50944778605189 0.0 86-87 0.0 1.8619078783839013E-4 0.0 5.026592699273018 0.0 88-89 0.0 1.8619078783839013E-4 0.0 5.590424952544623 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGATG 25 0.0016177732 37.99213 58-59 GGTATCA 4290 0.0 37.132866 1 GTATCAA 7155 0.0 35.42326 1 ACCGTCA 55 0.00257922 34.546352 8 CATTCCG 270 0.0 31.66749 9 ATCAACG 8775 0.0 28.599276 3 TCAACGC 8795 0.0 28.538237 4 CAACGCA 8880 0.0 28.263746 5 GTACATG 5265 0.0 28.179102 1 AACGCAG 9030 0.0 27.722206 6 TATCAAC 9145 0.0 27.44345 2 CTCCCGC 245 0.0 27.14356 7 TACATGG 5620 0.0 25.626827 2 ACATGGG 5305 0.0 25.624115 3 ACGCAGA 9950 0.0 24.872505 7 CGCAGAG 9975 0.0 24.76255 8 GGTACTC 270 1.8189894E-12 24.64521 3 CATGGGG 1530 0.0 24.545094 4 ATGGGTA 605 0.0 24.356512 5 GCAGAGT 10455 0.0 23.262203 9 >>END_MODULE