FastQCFastQC Report
Thu 26 May 2016
SRR937190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074167
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101310.9431494357953651No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84820.7896351312226124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65790.6124745965943843No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25520.2375794452817858No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21120.19661747195733997No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18030.1678509952363087No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16380.15249025523964152No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT13530.12595806797267092No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT12340.1148797160962867No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA11970.11143518652127649No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59450.033.4143831
GTATCAA86700.029.7090821
CATTCCG1951.6370905E-1129.2302979
CTCCCGC3050.028.0323337
GGTACTC3250.026.3072663
ACTCCCG3400.025.1454816
TACTCCC3650.024.7256265
CGTATAG2351.7462298E-1024.2673551
GGATATG7050.024.2673531
ATCAACG109950.023.4148123
TCAACGC110500.023.2982654
TATCAAC111350.023.1630742
CAACGCA112050.022.975985
AACGCAG114850.022.4561446
GAGTACT74300.022.43907212-13
AGTACTT77750.022.23758112-13
GATTGGC7050.022.2336859
TCCGGTG3059.094947E-1221.8029252
AGAGTAC100500.021.69367810-11
GTGGTAC4400.021.6016221
Produced by FastQC (version 0.11.5)