FastQCFastQC Report
Thu 26 May 2016
SRR937189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118335
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104770.93683914032915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74750.6684043689949791No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58050.5190752323767028No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20590.18411298939941967No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16670.14906088068423148No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16220.14503704167355935No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15470.13833064332243916No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13180.11785377369035219No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT13130.11740668046694416No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11850.10596109394769905No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT11780.10533516343492781No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAT200.002072663771.26216
GGTATCA42800.038.874451
GTATCAA73500.037.771611
ATCAACG90800.030.4607683
TCAACGC91050.030.378494
CAACGCA91500.030.2796695
AACGCAG93350.029.6193856
TATCAAC94800.029.17552
TTCGATA1004.9351675E-528.5150664
GTACATG53850.027.4545731
TAATGCG350.008394537527.14199434-35
ACGCAGA104050.026.4821537
CGCAGAG104050.026.4353088
ACATGGG53400.025.808363
GTAAGAC1858.922143E-925.6880973
CATGGGG14250.025.0132164
CTCCCGC2850.025.0042467
TACATGG57600.024.9990672
GCAGAGT110500.024.7202859
GAGTACT64650.023.99178512-13
Produced by FastQC (version 0.11.5)