##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937189_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1118335 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28881864557579 33.0 31.0 34.0 31.0 34.0 2 32.434809784188104 34.0 31.0 34.0 31.0 34.0 3 32.47279750700819 34.0 31.0 34.0 31.0 34.0 4 36.06343805746936 37.0 35.0 37.0 35.0 37.0 5 36.074474106595964 37.0 37.0 37.0 35.0 37.0 6 36.16248351343739 37.0 37.0 37.0 35.0 37.0 7 36.132040041669086 37.0 37.0 37.0 35.0 37.0 8 36.13607729347646 37.0 37.0 37.0 35.0 37.0 9 37.858484264554 39.0 38.0 39.0 35.0 39.0 10-11 37.80767122552723 39.0 38.0 39.0 35.0 39.0 12-13 37.74916684177818 39.0 38.0 39.0 35.0 39.0 14-15 39.05060111683887 40.0 38.5 41.0 36.0 41.0 16-17 39.01053441052994 40.0 38.0 41.0 36.0 41.0 18-19 39.02988549942549 40.0 38.5 41.0 36.0 41.0 20-21 39.00710878225219 40.0 39.0 41.0 36.0 41.0 22-23 38.881498388228934 40.0 38.5 41.0 36.0 41.0 24-25 38.772898997169904 40.0 38.0 41.0 35.0 41.0 26-27 38.623692364094836 40.0 38.0 41.0 35.0 41.0 28-29 38.465300200744856 40.0 38.0 41.0 35.0 41.0 30-31 38.42386091824007 40.0 38.0 41.0 35.0 41.0 32-33 38.254051335243915 40.0 38.0 41.0 35.0 41.0 34-35 38.08742103215941 40.0 38.0 41.0 34.5 41.0 36-37 38.05586966338352 40.0 38.0 41.0 34.5 41.0 38-39 38.03203244108429 40.0 38.0 41.0 34.5 41.0 40-41 37.90705870781116 40.0 38.0 41.0 34.5 41.0 42-43 37.67579973800337 40.0 38.0 41.0 33.5 41.0 44-45 37.39572400041133 40.0 38.0 41.0 33.0 41.0 46-47 37.01093768861745 40.0 37.5 41.0 33.0 41.0 48-49 36.67528513370323 40.0 37.0 41.0 31.5 41.0 50-51 35.7792383319846 39.0 36.0 40.0 30.5 40.5 52-53 35.9044235403524 39.0 36.5 40.0 31.0 40.5 54-55 36.307690450535844 40.0 37.0 41.0 31.0 41.0 56-57 36.16131972977686 40.0 36.5 41.0 30.5 41.0 58-59 35.99858942088015 39.5 36.0 41.0 30.0 41.0 60-61 35.78609808331135 39.0 36.0 41.0 29.0 41.0 62-63 35.53123750933307 39.0 35.5 40.5 28.5 41.0 64-65 35.24093406716234 39.0 35.0 40.0 28.0 41.0 66-67 34.889158436425575 38.0 35.0 40.0 27.5 41.0 68-69 34.42104870186483 37.5 35.0 40.0 26.0 41.0 70-71 34.03504048429138 37.0 34.0 39.5 26.0 41.0 72-73 33.61998640836601 36.5 34.0 39.0 25.5 40.5 74-75 33.15419395798218 36.0 34.0 39.0 24.5 40.0 76-77 32.7138607841121 35.5 33.5 38.0 22.5 39.5 78-79 32.24138071329253 35.0 33.0 37.0 20.5 39.0 80-81 31.77685174835805 35.0 33.0 37.0 19.0 39.0 82-83 31.32540338986082 35.0 32.0 36.0 16.0 38.5 84-85 30.861883067238352 35.0 32.0 36.0 8.5 37.0 86-87 30.46826889974829 34.5 31.0 35.0 4.5 37.0 88-89 30.101529058824056 34.0 31.0 35.0 2.0 36.0 90-91 29.712439921848105 34.0 31.0 35.0 2.0 36.0 92-93 29.36233329011432 34.0 30.5 35.0 2.0 36.0 94-95 28.916208917721434 34.0 30.0 35.0 2.0 35.0 96-97 28.431837508438882 34.0 29.0 35.0 2.0 35.0 98-99 27.905461243723927 34.0 29.0 35.0 2.0 35.0 100-101 26.131231697121166 32.5 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1953848579945543 1101 2 0.1329919185289441 1101 3 0.2914265991701015 1101 4 0.13131271217083906 1101 5 0.19977539221046214 1101 6 0.13609967547098023 1101 7 0.22104111560694406 1101 8 0.20402426013910713 1101 9 0.24238261865974664 1101 10-11 0.18307987540009663 1101 12-13 0.2672970760922837 1101 14-15 0.28970798159389943 1101 16-17 0.3167261411338984 1101 18-19 0.34512177015773915 1101 20-21 0.33901170085814414 1101 22-23 0.253696271357164 1101 24-25 0.24930595489772145 1101 26-27 0.31445460920053137 1101 28-29 0.2861209432740921 1101 30-31 0.32644771590254607 1101 32-33 0.38044333287526655 1101 34-35 0.26925961454299596 1101 36-37 0.32497527270914617 1101 38-39 0.3789946280791767 1101 40-41 0.509761764088239 1101 42-43 0.5528848913823694 1101 44-45 0.3719543311113398 1101 46-47 0.5388610057951126 1101 48-49 0.6384618358433798 1101 50-51 0.5244985824802058 1101 52-53 0.5913456910139345 1101 54-55 0.698094198204906 1101 56-57 0.7649647632152394 1101 58-59 0.8344450680664579 1101 60-61 0.7225611604849504 1101 62-63 0.8101790624091976 1101 64-65 0.7324396601971443 1101 66-67 0.7950671254287727 1101 68-69 0.7382468198125238 1101 70-71 0.8146802150625589 1101 72-73 0.7332930457605187 1101 74-75 0.8038483662473794 1101 76-77 0.8556458410962264 1101 78-79 0.825458852809696 1101 80-81 0.9244798563462773 1101 82-83 0.9697296388640275 1101 84-85 0.919173215965511 1101 86-87 1.1023452024010716 1101 88-89 1.0301849639304734 1101 90-91 1.0678157304474034 1101 92-93 1.1877874371030792 1101 94-95 0.9713242045893544 1101 96-97 1.0134466630576853 1101 98-99 1.3173912642842502 1101 100-101 1.804924801064029 1102 1 -0.07311435522338172 1102 2 -0.05914026085736168 1102 3 0.030888636935635816 1102 4 -0.1125503877504812 1102 5 -0.03788688158969222 1102 6 -0.029852330823274542 1102 7 -0.013115060632237885 1102 8 0.012595267220142148 1102 9 0.1006290781404644 1102 10-11 -0.04462714662980005 1102 12-13 0.08582825233146707 1102 14-15 0.11631842612576548 1102 16-17 0.13254046056741942 1102 18-19 0.1329241895125719 1102 20-21 0.1529131564049564 1102 22-23 0.1973872627025628 1102 24-25 0.14906992027930954 1102 26-27 0.21417677678510927 1102 28-29 0.1521958842654314 1102 30-31 0.156965025107894 1102 32-33 0.12436790014513832 1102 34-35 0.093851471348664 1102 36-37 0.2886234827799541 1102 38-39 0.3219947264269294 1102 40-41 0.20538037148399724 1102 42-43 0.2169781250566345 1102 44-45 0.1934482335503631 1102 46-47 0.24896132050794506 1102 48-49 0.4275944086207275 1102 50-51 0.35049435352662783 1102 52-53 0.24051711115555463 1102 54-55 0.3220467945857095 1102 56-57 0.3627052234827417 1102 58-59 0.3851531719216865 1102 60-61 0.392542966149783 1102 62-63 0.3530832716932224 1102 64-65 0.46631554532696384 1102 66-67 0.6128951152006081 1102 68-69 0.49261100155918314 1102 70-71 0.44958875164793 1102 72-73 0.4351911574835725 1102 74-75 0.5013355810388873 1102 76-77 0.3928139174140881 1102 78-79 0.39042984022118077 1102 80-81 0.40689035988679123 1102 82-83 0.4053462793046272 1102 84-85 0.4769623583730649 1102 86-87 0.3788376296237317 1102 88-89 0.42214011735297774 1102 90-91 0.395434239495394 1102 92-93 0.35730454961291613 1102 94-95 0.3493164351164957 1102 96-97 0.4489133231678366 1102 98-99 0.4749394546855079 1102 100-101 0.5395137074369636 1103 1 0.04836685349342673 1103 2 -0.013650992198734002 1103 3 0.003710920250370009 1103 4 -0.008766057521583548 1103 5 -0.060014555276403314 1103 6 -0.05577954433397281 1103 7 -0.020725576065970586 1103 8 0.0043243351578610145 1103 9 0.03778771993506069 1103 10-11 0.08901573436483545 1103 12-13 0.04928319762266398 1103 14-15 0.07302568602000292 1103 16-17 0.11971751397711472 1103 18-19 0.0868228204203021 1103 20-21 0.043015826889650555 1103 22-23 0.016177516668491876 1103 24-25 0.06817317170192183 1103 26-27 -0.03897749882646906 1103 28-29 0.0037691053145962883 1103 30-31 0.06431343233165165 1103 32-33 0.0326657634829175 1103 34-35 0.11763733397285137 1103 36-37 0.2873252351997664 1103 38-39 0.19490798141750787 1103 40-41 0.2454519366313832 1103 42-43 0.2951485823050959 1103 44-45 0.36563400102881616 1103 46-47 0.5021393253750119 1103 48-49 0.4440413557233498 1103 50-51 0.4172967820300926 1103 52-53 0.3951904021917301 1103 54-55 0.4448402597202801 1103 56-57 0.5943064736428454 1103 58-59 0.6332353906471013 1103 60-61 0.5767434560588427 1103 62-63 0.6069720106462526 1103 64-65 0.6701647164612083 1103 66-67 0.6725896310551818 1103 68-69 0.6321890553714127 1103 70-71 0.6306967191885917 1103 72-73 0.5761006226547423 1103 74-75 0.4730531674476808 1103 76-77 0.5093780241419879 1103 78-79 0.4993148168006911 1103 80-81 0.5056595262637522 1103 82-83 0.4851835023298925 1103 84-85 0.34226023772095004 1103 86-87 0.42722329692470495 1103 88-89 0.4728143712823112 1103 90-91 0.6820599164939125 1103 92-93 0.6591584048450194 1103 94-95 0.7356621640792262 1103 96-97 0.8207047275738155 1103 98-99 0.8113522545318084 1103 100-101 1.259294643524644 1104 1 0.06185951237243614 1104 2 0.06543795296988719 1104 3 0.1318158788208379 1104 4 -0.03799968171710333 1104 5 0.014424919253883672 1104 6 -0.020976482666299034 1104 7 -0.03179056035715888 1104 8 0.034705802705197186 1104 9 0.05104742583898059 1104 10-11 0.03763663019029906 1104 12-13 0.007064842281955919 1104 14-15 0.13191935680612232 1104 16-17 0.11883928690154022 1104 18-19 0.07914129477573084 1104 20-21 0.13857815732188072 1104 22-23 0.07270245617404214 1104 24-25 0.12721039341335683 1104 26-27 0.17398043990633028 1104 28-29 0.061205832917551106 1104 30-31 0.04659687913319743 1104 32-33 -0.062198554100241665 1104 34-35 0.032760099626671035 1104 36-37 -0.15819535547421992 1104 38-39 -0.03771953636419312 1104 40-41 -0.11368596814556753 1104 42-43 0.11565775095063913 1104 44-45 0.17251824088577195 1104 46-47 0.2599621457417598 1104 48-49 0.229102752651535 1104 50-51 0.09048208963518789 1104 52-53 0.1336510511885649 1104 54-55 0.10197183584740088 1104 56-57 0.09657706008645306 1104 58-59 0.004397166053365709 1104 60-61 0.21909887574083342 1104 62-63 0.14203086934588782 1104 64-65 0.08483172907902059 1104 66-67 0.09731366920757267 1104 68-69 0.22379738978584385 1104 70-71 0.09813886167909658 1104 72-73 0.16429898803556142 1104 74-75 0.2347724506519384 1104 76-77 0.2476237697889232 1104 78-79 0.18461480721182966 1104 80-81 0.27600483815563237 1104 82-83 0.3604524135426601 1104 84-85 0.29926659457331795 1104 86-87 0.31020052619442495 1104 88-89 0.17759582980483657 1104 90-91 0.402247576190792 1104 92-93 0.559316057040018 1104 94-95 0.6079086091491384 1104 96-97 0.49881532665215644 1104 98-99 0.5351574539519639 1104 100-101 0.7891171367419361 1105 1 0.03756823944303278 1105 2 0.08830445775324591 1105 3 0.011550234164452888 1105 4 0.041746376034275556 1105 5 -0.0015927513818496664 1105 6 0.012398784294880727 1105 7 0.03416713543875005 1105 8 0.04130303508576105 1105 9 0.1499795811299336 1105 10-11 0.08991274850163933 1105 12-13 0.20966716520989337 1105 14-15 0.25649441312395993 1105 16-17 0.2783255512004317 1105 18-19 0.23043148523242252 1105 20-21 0.23372897210202126 1105 22-23 0.31061386894722176 1105 24-25 0.25479735693111394 1105 26-27 0.1756100001883567 1105 28-29 0.17011429083276397 1105 30-31 0.08142763306081946 1105 32-33 0.183862122884058 1105 34-35 0.2743007216096416 1105 36-37 0.3066418347776789 1105 38-39 0.25108418500148133 1105 40-41 0.38556507775712845 1105 42-43 0.3589113128710082 1105 44-45 0.38586483694790985 1105 46-47 0.4562012844445462 1105 48-49 0.3277818433744457 1105 50-51 0.19074685661099977 1105 52-53 0.1935893209875701 1105 54-55 0.33492581612884464 1105 56-57 0.3739314382497625 1105 58-59 0.4580000448456758 1105 60-61 0.5209639469792435 1105 62-63 0.5369739663881106 1105 64-65 0.385039446816954 1105 66-67 0.4524554146216886 1105 68-69 0.5309636517442371 1105 70-71 0.4526179582532208 1105 72-73 0.5861093505176385 1105 74-75 0.6227701686824219 1105 76-77 0.6260079598360306 1105 78-79 0.7733878097571036 1105 80-81 0.5955025123020938 1105 82-83 0.532049207082931 1105 84-85 0.5388324352450375 1105 86-87 0.5846205256093064 1105 88-89 0.7232030384880268 1105 90-91 0.693416646727055 1105 92-93 0.7115746217397074 1105 94-95 0.7709962894393598 1105 96-97 0.8438125473303089 1105 98-99 1.0363130667192912 1105 100-101 1.1823472938467567 1106 1 -0.05559185173485304 1106 2 -0.014101346527851888 1106 3 -0.0016818461523513406 1106 4 -9.836929135076389E-4 1106 5 0.06660755149928121 1106 6 0.03917146980957398 1106 7 0.09748246103596614 1106 8 0.06354441818022849 1106 9 -0.009900269749707036 1106 10-11 -0.04937996447930004 1106 12-13 -0.14406570366389104 1106 14-15 -0.08646140573682004 1106 16-17 -0.21172483492598815 1106 18-19 -0.1672495614946783 1106 20-21 -0.037159635992992435 1106 22-23 0.027563924914389304 1106 24-25 -0.04805179509578039 1106 26-27 0.026435711849281063 1106 28-29 0.08165199263594758 1106 30-31 0.12198266430523574 1106 32-33 0.17566928229170742 1106 34-35 0.020785764110200944 1106 36-37 -0.05041300677410021 1106 38-39 -0.03428972896899296 1106 40-41 0.25603103184837295 1106 42-43 0.1675638053695252 1106 44-45 0.07241766713067932 1106 46-47 0.23386113576314926 1106 48-49 0.1596235550604561 1106 50-51 -0.03289009990392344 1106 52-53 0.21027098539151723 1106 54-55 0.2021917652034233 1106 56-57 0.35251999962708425 1106 58-59 0.3269917265884601 1106 60-61 0.28184932559633324 1106 62-63 0.218119459071616 1106 64-65 0.31189333959201093 1106 66-67 0.4118411240251163 1106 68-69 0.2535148138859782 1106 70-71 0.2351072900021265 1106 72-73 0.39464802743503213 1106 74-75 0.40710406534540056 1106 76-77 0.2742852058125038 1106 78-79 0.21629220780437208 1106 80-81 0.2741130215876204 1106 82-83 0.04451844081874512 1106 84-85 0.08743827276153837 1106 86-87 0.06530918826055299 1106 88-89 0.1897252670159162 1106 90-91 0.43673680086407884 1106 92-93 0.40379722369558024 1106 94-95 0.33251282718819297 1106 96-97 0.6123009948920561 1106 98-99 0.4184712985958079 1106 100-101 0.6689321052671957 1107 1 1.0942950866166257E-4 1107 2 -0.07719007882653983 1107 3 -0.09343169486977132 1107 4 -0.0725012488600214 1107 5 -0.022845170122693048 1107 6 0.0423186461069065 1107 7 -0.1369042727122931 1107 8 0.021672639751407985 1107 9 0.03557486973875257 1107 10-11 0.04419275367767028 1107 12-13 0.15317169875027048 1107 14-15 0.05359884555118555 1107 16-17 0.0929782305483684 1107 18-19 0.07621256924961983 1107 20-21 0.11517771887076833 1107 22-23 0.004351474311036441 1107 24-25 0.07095228875496673 1107 26-27 0.1730261981534511 1107 28-29 0.048273850990575795 1107 30-31 0.1526040664751065 1107 32-33 0.25778004669409427 1107 34-35 0.3107363627903794 1107 36-37 0.21638029478832976 1107 38-39 0.2200990239577223 1107 40-41 0.24941261357555788 1107 42-43 0.216671596469304 1107 44-45 0.2656704950711273 1107 46-47 0.2610137887704411 1107 48-49 0.27447866342614446 1107 50-51 0.21775008561120757 1107 52-53 0.32690128814987673 1107 54-55 0.3237780916260604 1107 56-57 0.26478731891216967 1107 58-59 0.39536646866022807 1107 60-61 0.3409374047780389 1107 62-63 0.39831804901199774 1107 64-65 0.2509291070948336 1107 66-67 0.34810363716690773 1107 68-69 0.1838939769614356 1107 70-71 0.22980009131932633 1107 72-73 0.21109753893717453 1107 74-75 0.23148726553137067 1107 76-77 0.25910054758026746 1107 78-79 0.18337243213260024 1107 80-81 0.20786901134963998 1107 82-83 0.05873495283753272 1107 84-85 0.20594628684343874 1107 86-87 0.004534097942823223 1107 88-89 0.24514579520558044 1107 90-91 0.17264602009637997 1107 92-93 0.07418472523021435 1107 94-95 0.2392741459610086 1107 96-97 0.34016434885964486 1107 98-99 0.22329775830914755 1107 100-101 0.5577108949192784 1108 1 -0.10909906233518285 1108 2 -0.1886329549809389 1108 3 -0.1317470069705351 1108 4 -0.1472253089067337 1108 5 -0.03703138025541364 1108 6 0.0586019584633064 1108 7 0.0010476560714423044 1108 8 0.025388132094086302 1108 9 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-0.510103396502867 2316 82-83 -0.70913360239539 2316 84-85 -0.8821547732174153 2316 86-87 -1.1138393639500954 2316 88-89 -1.0671252543426561 2316 90-91 -0.9409562417372612 2316 92-93 -0.827151233941926 2316 94-95 -0.8123077250996538 2316 96-97 -0.7783874107302218 2316 98-99 -0.9997711850107116 2316 100-101 -1.743447808555441 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3836.0 3 490.0 4 293.0 5 341.0 6 377.0 7 395.0 8 542.0 9 659.0 10 866.0 11 1364.0 12 2343.0 13 3588.0 14 4241.0 15 6072.0 16 9101.0 17 14596.0 18 11807.0 19 5453.0 20 4371.0 21 3930.0 22 3980.0 23 4270.0 24 5171.0 25 5664.0 26 6402.0 27 7519.0 28 9173.0 29 11221.0 30 14070.0 31 18452.0 32 25306.0 33 35985.0 34 54614.0 35 90732.0 36 148560.0 37 263448.0 38 290470.0 39 48619.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.45736093382645 22.9638925342502 16.138789445027005 21.439957086896342 2 13.865798653144306 22.72590320931859 41.689398219137544 21.718899918399558 3 16.56354411582725 28.427121051527198 32.35169632112542 22.657638511520133 4 10.497601219566874 19.397031789445364 41.97507061830247 28.130296372685287 5 10.62586355147058 40.14966282826163 36.79964350622743 12.42483011404035 6 25.869377639314123 41.70691998706159 19.3654087560044 13.05829361761989 7 24.363662757615213 35.446258233964215 23.857191630143458 16.33288737827712 8 23.799790858903236 36.07235887680299 21.884545870807433 18.24330439348634 9 25.204991662343275 17.047887091730352 23.490597399152882 34.25652384677349 10-11 21.728299144345588 28.991974851457908 30.190303240482486 19.08942276371402 12-13 22.953470533309197 27.87049997401289 29.77943219067726 19.396597302000647 14-15 20.82904766853862 28.65299912912902 28.99391107081292 21.524042131519437 16-17 18.028529082854234 31.825545885962526 29.981209408020188 20.164715623163058 18-19 17.735447734950476 30.249519878400555 33.52518784883573 18.489844537813244 20-21 18.898619858854392 29.396509125901648 33.43053352286549 18.27433749237847 22-23 18.668846295825077 29.221963780763595 33.060133035627885 19.049056887783443 24-25 18.291749873901285 29.76821129754907 33.381085102970914 18.558953725578732 26-27 18.14965496323834 29.812762171265287 33.547841677788576 18.489741187707796 28-29 17.75862555604489 29.564944295827306 33.88508099472281 18.791349153404997 30-31 18.262687487342532 29.50538648065939 33.81345087827145 18.418475153726625 32-33 18.04149971033272 29.80687503892474 33.346685359503304 18.804939891239233 34-35 18.465226528539564 29.612297039576696 33.28118623011836 18.641290201765383 36-37 18.584979072018864 29.200032793069013 33.08959541327107 19.125392721641052 38-39 17.697073131400003 30.441673161860223 33.02523718655092 18.836016520188853 40-41 17.973646583265545 30.001379666228278 33.47849691635639 18.546476834149786 42-43 17.96233102221203 29.75294683644554 32.88738457458674 19.39733756675569 44-45 18.31699913755446 29.908939191989337 32.60877454329589 19.165287127160312 46-47 18.55678554581508 30.031756953805257 32.15762272165471 19.253834778724947 48-49 18.195498827329605 30.343064892242005 32.06356388757597 19.39787239285242 50-51 18.344220215055305 30.201026842260664 31.85630481058439 19.59844813209964 52-53 18.414307696306036 30.910330896619335 31.40318520370624 19.27217620336838 54-55 18.75473831931478 30.431883803416426 31.42036666302238 19.39301121424641 56-57 18.776768957192065 30.823281210106916 30.977366485556747 19.422583347144272 58-59 18.674982622840393 30.29362921082615 31.51406116760181 19.517326998731647 60-61 18.867368447455572 31.02329743806117 31.072029726696066 19.037304387787188 62-63 18.58755857588595 30.65878219490149 31.578662118254535 19.174997110958024 64-65 18.66085649429643 30.345251084368435 31.443862770285886 19.550029651049254 66-67 18.70067215108505 30.60471200084215 30.76754233700728 19.92707351106552 68-69 18.648258365970683 30.21858662662993 31.3258527216503 19.807302285749095 70-71 18.786637097940954 30.389121981743656 31.562718501222154 19.26152241909324 72-73 18.96889952903324 29.938263170650526 31.49402589443589 19.598811405880344 74-75 18.50901067161665 30.745400597725205 30.99261623031771 19.752972500340434 76-77 18.420598751104844 30.345222431730306 31.507163518893584 19.727015298271265 78-79 18.372296590830693 30.226710520303513 31.182148786394503 20.21884410247129 80-81 18.43074880585944 30.43342912735469 30.94019980578419 20.19562226100168 82-83 18.693736574614345 30.420047978604497 31.07427724107345 19.81193820570771 84-85 18.50404792564806 30.001679637198365 30.973988018587985 20.52028441856559 86-87 18.306099166379624 30.30873650814696 31.198884146431595 20.186280179041823 88-89 18.843969890215035 29.769965829945296 31.22228274600379 20.16378153383588 90-91 18.361446020594467 29.729480336348864 31.59281934955591 20.316254293500755 92-93 18.790504113405916 29.954778292684566 31.432199916231212 19.822517677678306 94-95 18.594427325020146 29.3218333805928 31.518166673162046 20.56557262122501 96-97 18.878379921925568 29.244854948633474 31.222874957726482 20.65389017171448 98-99 18.620612111606754 29.390307381767457 31.065008972655573 20.924071533970213 100-101 18.50578851755904 29.05765048174942 31.634266375717967 20.802294624973573 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2548.0 1 1557.0 2 649.0 3 779.0 4 665.5 5 677.0 6 1288.5 7 2546.5 8 3942.5 9 4196.0 10 3236.0 11 2452.5 12 2357.0 13 2328.5 14 2277.0 15 2277.0 16 2238.5 17 2339.5 18 2540.0 19 2896.0 20 3366.5 21 3833.5 22 4408.0 23 5113.0 24 5838.0 25 7343.5 26 10751.5 27 14336.5 28 15640.5 29 17615.5 30 21777.0 31 25907.5 32 32115.0 33 40416.5 34 48424.5 35 52679.5 36 57771.0 37 63371.5 38 60778.5 39 55914.0 40 51472.5 41 47305.5 42 43278.5 43 41852.0 44 42025.0 45 40123.0 46 36388.5 47 32257.0 48 31457.0 49 28718.0 50 23441.0 51 19116.0 52 16132.0 53 14014.5 54 11912.0 55 10149.0 56 8664.0 57 7124.5 58 5608.5 59 4446.0 60 3583.5 61 2792.5 62 1982.5 63 1354.0 64 937.5 65 650.0 66 444.5 67 310.5 68 207.0 69 134.5 70 113.5 71 91.5 72 57.0 73 64.0 74 61.5 75 39.0 76 29.0 77 22.5 78 18.0 79 10.0 80 6.0 81 5.5 82 4.0 83 3.5 84 2.0 85 0.5 86 0.5 87 1.0 88 1.5 89 1.0 90 1.0 91 1.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31511130385796743 2 0.2812216375236401 3 0.2822946612598193 4 0.2847983833109041 5 0.2707596560958926 6 0.20369567258469062 7 0.21263753705285088 8 0.20941846584431317 9 0.20691474379322833 10-11 0.21509654978159493 12-13 0.21433649130180132 14-15 0.19770462339102324 16-17 0.21022323364644763 18-19 0.1977493327133641 20-21 0.20150491578999138 22-23 0.20056602002083454 24-25 0.1918924114867191 26-27 0.20517108022193709 28-29 0.2061546853134347 30-31 0.21397881672307492 32-33 0.21456003791350534 34-35 0.2022202649474442 36-37 0.20078956663253855 38-39 0.19001461994840543 40-41 0.18983578265904225 42-43 0.1911770623292663 44-45 0.20767480227302193 46-47 0.19685514626654804 48-49 0.20499224293257387 50-51 0.21366585146668934 52-53 0.22247358796782718 54-55 0.21755556251033906 56-57 0.1850965944909173 58-59 0.1723097283014481 60-61 0.1816986859930164 62-63 0.18312938430792206 64-65 0.18035740632279237 66-67 0.1915794462303335 68-69 0.1812515927696084 70-71 0.1846942105898501 72-73 0.1990906123835881 74-75 0.18804740976541018 76-77 0.19926944967295132 78-79 0.19654218101016244 80-81 0.18339764024196686 82-83 0.19783875135804566 84-85 0.18084920886854117 86-87 0.18415769872176047 88-89 0.19251834199949033 90-91 0.1889415962122262 92-93 0.19381491234737355 94-95 0.1928313072558759 96-97 0.18710851399625336 98-99 0.19368078438035113 100-101 0.1776748469823443 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1118335.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.647980134986156 #Duplication Level Percentage of deduplicated Percentage of total 1 78.40048539866557 32.652218584568956 2 12.352346223812905 10.289005402996624 3 3.818142805474931 4.770538071448808 4 1.564622599107627 2.6065348370553942 5 0.8252738555242256 1.7185494570398192 6 0.47186302586208534 1.1791285156523155 7 0.30318382931879784 0.8838895870502829 8 0.22159167148488473 0.7383076425664686 9 0.17124905251620048 0.6418959423596927 >10 1.3722956314572978 12.06431353562955 >50 0.2649079343413343 7.78098775547838 >100 0.2151777695641973 16.92684930816924 >500 0.015645395562552043 4.199333201173176 >1k 0.002571845845898966 1.4278946822082068 >5k 4.2864097431649434E-4 1.1853748440160647 >10k+ 2.1432048715824717E-4 0.9351786325870715 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10477 0.93683914032915 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7475 0.6684043689949791 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5805 0.5190752323767028 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2059 0.18411298939941967 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1667 0.14906088068423148 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1622 0.14503704167355935 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1547 0.13833064332243916 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1318 0.11785377369035219 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1313 0.11740668046694416 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1185 0.10596109394769905 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1178 0.10533516343492781 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 1.7883728936320513E-4 0.0 26-27 0.0 0.0 0.0 6.259305127712179E-4 0.0 28-29 0.0 0.0 0.0 0.0016989542489504487 0.0 30-31 0.0 0.0 0.0 0.005275700036214552 0.0 32-33 0.0 0.0 0.0 0.010327853460725095 0.0 34-35 0.0 0.0 0.0 0.018017856903342917 0.0 36-37 0.0 0.0 0.0 0.0313859442832425 0.0 38-39 0.0 0.0 0.0 0.0524440351057599 0.0 40-41 0.0 0.0 0.0 0.07940375647726307 0.0 42-43 0.0 0.0 0.0 0.10658702446047025 0.0 44-45 0.0 0.0 0.0 0.14700425185655464 0.0 46-47 0.0 0.0 0.0 0.19788346068038648 0.0 48-49 0.0 0.0 0.0 0.2516687754563704 0.0 50-51 0.0 0.0 0.0 0.30929909195366323 0.0 52-53 0.0 0.0 0.0 0.3785091229372236 0.0 54-55 0.0 0.0 0.0 0.4625179396155893 0.0 56-57 0.0 0.0 0.0 0.5637845547175042 0.0 58-59 0.0 0.0 0.0 0.6832031546897843 0.0 60-61 0.0 8.941864468160257E-5 0.0 0.8209525768217931 0.0 62-63 0.0 8.941864468160257E-5 0.0 0.9582549057303938 0.0 64-65 0.0 8.941864468160257E-5 0.0 1.1227852119445425 0.0 66-67 0.0 8.941864468160257E-5 0.0 1.3200427421121579 0.0 68-69 0.0 8.941864468160257E-5 0.0 1.5475237741821548 0.0 70-71 0.0 8.941864468160257E-5 0.0 1.8101463336120214 0.0 72-73 0.0 8.941864468160257E-5 0.0 2.0684320887748306 0.0 74-75 0.0 8.941864468160257E-5 0.0 2.361680534008146 0.0 76-77 0.0 8.941864468160257E-5 0.0 2.6860019582683186 0.0 78-79 0.0 8.941864468160257E-5 0.0 3.065002883751291 0.0 80-81 0.0 8.941864468160257E-5 0.0 3.4742273111366453 0.0 82-83 0.0 8.941864468160257E-5 0.0 3.8936901733380425 0.0 84-85 0.0 8.941864468160257E-5 0.0 4.332333334823645 0.0 86-87 0.0 8.941864468160257E-5 0.0 4.822347507678826 0.0 88-89 0.0 8.941864468160257E-5 0.0 5.357965189321625 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCTAT 20 0.0020726637 71.2621 6 GGTATCA 4280 0.0 38.87445 1 GTATCAA 7350 0.0 37.77161 1 ATCAACG 9080 0.0 30.460768 3 TCAACGC 9105 0.0 30.37849 4 CAACGCA 9150 0.0 30.279669 5 AACGCAG 9335 0.0 29.619385 6 TATCAAC 9480 0.0 29.1755 2 TTCGATA 100 4.9351675E-5 28.515066 4 GTACATG 5385 0.0 27.454573 1 TAATGCG 35 0.0083945375 27.141994 34-35 ACGCAGA 10405 0.0 26.482153 7 CGCAGAG 10405 0.0 26.435308 8 ACATGGG 5340 0.0 25.80836 3 GTAAGAC 185 8.922143E-9 25.688097 3 CATGGGG 1425 0.0 25.013216 4 CTCCCGC 285 0.0 25.004246 7 TACATGG 5760 0.0 24.999067 2 GCAGAGT 11050 0.0 24.720285 9 GAGTACT 6465 0.0 23.991785 12-13 >>END_MODULE