FastQCFastQC Report
Thu 26 May 2016
SRR937189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118335
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107380.9601774065910482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89460.7999391953216165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68870.6158262059221968No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27640.24715313389994947No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21660.19368078438035113No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19800.17704891646957308No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18390.1644408875694671No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT13250.1184797042031234No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA12530.112041561786048No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT12240.10944842109028154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64400.037.052921
GTATCAA93100.029.4598661
CATTCCG2151.8189894E-1228.7222299
CATATAG5200.025.5826553
TCAACGC115550.023.6833674
CAACGCA116900.023.5724335
ATCAACG116250.023.499893
TATCAAC117700.023.2911152
AACGCAG119350.023.1221456
GAGTACT80450.023.02776512-13
GACATAT6500.021.9387821
CTGGTCG7000.021.0366659
AGTACTT83750.020.98602512-13
GTACTTT87650.020.94459314-15
CAGCGCA1604.9873946E-520.7821889
ATATGAG9150.020.769683
ACGCAGA134000.020.560637
AGAGTAC110950.020.48650410-11
CGCAGAG133900.020.4695578
GGTACTC3503.6379788E-1220.3617063
Produced by FastQC (version 0.11.5)