FastQCFastQC Report
Thu 26 May 2016
SRR937188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162852
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109980.9457781385765341No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78390.6741184604747638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59880.5149408523182658No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22270.19151190349244787No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17380.14946012046244922No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16740.14395641061803222No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16700.14361242875275618No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13600.1169538341938613No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT13560.11660985232858523No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12040.10353854144809485No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46350.040.08081
CATTCCG3050.035.824589
GTATCAA75700.035.587421
ATCAACG92900.028.8426573
TCAACGC93350.028.7048574
CAACGCA95000.028.2075165
TATCAAC96550.027.8506952
AACGCAG97050.027.7084056
GCGCACT350.00839828127.13955786-87
CGCCGCA350.00839828127.13955786-87
GTACATG57800.027.1266061
TAGGCAT4800.026.7258725
ACATGGG58400.025.0558853
TACATGG61800.024.9842762
CGCAGAG107350.024.9170688
ACGCAGA107050.024.8970587
CATGGGA36350.024.3107934
GTGTACG1000.00165109223.7563291
TTCGAGA1000.001651921223.7542782
GAGTACT63650.023.58430512-13