##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937188_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1162852 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39714426255448 33.0 31.0 34.0 31.0 34.0 2 32.5394701991311 34.0 31.0 34.0 31.0 34.0 3 32.57446003446698 34.0 31.0 34.0 31.0 34.0 4 36.13243989776859 37.0 37.0 37.0 35.0 37.0 5 36.1676627808182 37.0 37.0 37.0 35.0 37.0 6 36.224644236755836 37.0 37.0 37.0 35.0 37.0 7 36.15350620715276 37.0 37.0 37.0 35.0 37.0 8 36.173777058473476 37.0 37.0 37.0 35.0 37.0 9 37.91513107428976 39.0 38.0 39.0 35.0 39.0 10-11 37.84971905281154 39.0 38.0 39.0 35.0 39.0 12-13 37.7603400948702 39.0 38.0 39.0 35.0 39.0 14-15 39.08782244000096 40.0 39.0 41.0 36.0 41.0 16-17 39.03972990543939 40.0 38.5 41.0 36.0 41.0 18-19 39.06932309528642 40.0 38.5 41.0 36.0 41.0 20-21 39.00239755360097 40.0 39.0 41.0 36.0 41.0 22-23 38.87295760767492 40.0 38.5 41.0 35.5 41.0 24-25 38.84653120087509 40.0 38.0 41.0 35.5 41.0 26-27 38.85956940350104 40.0 38.0 41.0 35.5 41.0 28-29 38.79492833137837 40.0 38.0 41.0 35.0 41.0 30-31 38.57459676725843 40.0 38.0 41.0 35.0 41.0 32-33 38.3368893891914 40.0 38.0 41.0 35.0 41.0 34-35 38.33244041374139 40.0 38.0 41.0 35.0 41.0 36-37 38.21635642368934 40.0 38.0 41.0 35.0 41.0 38-39 38.126209096256446 40.0 38.0 41.0 35.0 41.0 40-41 37.95450710838524 40.0 38.0 41.0 34.5 41.0 42-43 37.69886709572671 40.0 38.0 41.0 34.0 41.0 44-45 37.40646144135282 40.0 38.0 41.0 33.0 41.0 46-47 37.0131504267095 40.0 37.5 41.0 33.0 41.0 48-49 36.660402613574206 40.0 37.0 41.0 31.5 41.0 50-51 35.72156645901628 39.0 36.0 40.0 30.0 40.5 52-53 35.84422695235507 39.0 36.5 40.0 30.5 40.5 54-55 36.26769528710446 40.0 37.0 41.0 31.0 41.0 56-57 36.12374188632775 40.0 36.5 41.0 30.5 41.0 58-59 35.94323181281882 39.0 36.0 41.0 30.0 41.0 60-61 35.70652714188908 39.0 36.0 41.0 29.0 41.0 62-63 35.46125474264997 39.0 35.0 40.5 28.0 41.0 64-65 35.120264229669814 38.5 35.0 40.0 28.0 41.0 66-67 34.79597446622614 38.0 35.0 40.0 26.5 41.0 68-69 34.339383687691985 37.5 34.5 40.0 26.0 41.0 70-71 33.922513355095916 37.0 34.0 39.0 26.0 41.0 72-73 33.48297977730614 36.5 34.0 39.0 24.5 40.5 74-75 32.98610743241616 36.0 33.5 38.5 23.0 40.0 76-77 32.5107348140606 35.5 33.0 37.5 21.5 39.5 78-79 32.01872809265495 35.0 33.0 37.0 19.5 39.0 80-81 31.524841940332905 35.0 32.0 36.5 17.5 39.0 82-83 31.047995789661968 35.0 32.0 36.0 10.5 37.5 84-85 30.536878295776248 34.5 31.0 35.5 6.5 37.0 86-87 30.131079449491423 34.0 31.0 35.0 2.0 37.0 88-89 29.706092434806838 34.0 30.5 35.0 2.0 36.0 90-91 29.26017369364287 34.0 30.0 35.0 2.0 36.0 92-93 28.85621429038261 34.0 29.0 35.0 2.0 35.5 94-95 28.422216670737118 34.0 29.0 35.0 2.0 35.0 96-97 27.82009017484598 34.0 29.0 35.0 2.0 35.0 98-99 27.094856439168527 34.0 27.0 35.0 2.0 35.0 100-101 24.506500827276383 31.0 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19177466967977352 1101 2 0.1077074192449885 1101 3 0.11698758909881235 1101 4 0.14965951518233567 1101 5 0.053890717077358374 1101 6 -0.01028872568965511 1101 7 0.05969325816030846 1101 8 0.04576074782073647 1101 9 0.05107802763148328 1101 10-11 0.08505816441325464 1101 12-13 0.13496201815762277 1101 14-15 0.07180924449517789 1101 16-17 0.1324064981036841 1101 18-19 0.11701288839491752 1101 20-21 0.21945076742337477 1101 22-23 0.19676952588168461 1101 24-25 0.19609231376989555 1101 26-27 0.15476679510350522 1101 28-29 0.07371032736330818 1101 30-31 0.10450044032227623 1101 32-33 -0.0868763412451301 1101 34-35 0.07407380168078959 1101 36-37 0.1411158666461816 1101 38-39 0.09903079421988537 1101 40-41 0.08518042893073385 1101 42-43 -0.10052654314846166 1101 44-45 -0.03651197525699956 1101 46-47 0.0301275073981202 1101 48-49 -0.099055118809936 1101 50-51 0.09188348893817988 1101 52-53 0.038593043448948094 1101 54-55 0.00715633184351816 1101 56-57 0.09100364946832684 1101 58-59 -0.03202009282074414 1101 60-61 0.17205751200729225 1101 62-63 -0.13042494293566875 1101 64-65 -0.002389710732082051 1101 66-67 0.07556199528767849 1101 68-69 0.15353964719522395 1101 70-71 -0.01265622638388919 1101 72-73 0.025894023744541528 1101 74-75 -0.05343948446638791 1101 76-77 0.2442161194022816 1101 78-79 0.05140057198824266 1101 80-81 0.11874115473477787 1101 82-83 0.09434804001887187 1101 84-85 0.20208017856950988 1101 86-87 0.19488693590292883 1101 88-89 0.25577094225381103 1101 90-91 0.29929472962734494 1101 92-93 0.46246200479230026 1101 94-95 0.3984980083415799 1101 96-97 0.6136924887739355 1101 98-99 0.6539572546782892 1101 100-101 0.6400855081800145 1102 1 0.019807757915067725 1102 2 -0.05282857146708153 1102 3 -0.06107452390428847 1102 4 -0.10013731144304927 1102 5 -0.09966965443967268 1102 6 -0.10370010339863 1102 7 -0.043165681468174455 1102 8 -0.2483665741606913 1102 9 -0.11963356292579164 1102 10-11 -0.039882576685819515 1102 12-13 0.06516712651674794 1102 14-15 0.07201967406174248 1102 16-17 0.016363057701212824 1102 18-19 0.0501845698964658 1102 20-21 0.09326679877012367 1102 22-23 0.0030098509590814615 1102 24-25 0.020192545968036768 1102 26-27 0.13400562056789767 1102 28-29 0.0671628234933408 1102 30-31 -0.07149728607090822 1102 32-33 -0.1961255672513289 1102 34-35 -0.08490331712726373 1102 36-37 0.0866799146337911 1102 38-39 0.10241097030348101 1102 40-41 -0.05379499676586619 1102 42-43 -0.010901688272305421 1102 44-45 0.024319100594397014 1102 46-47 -0.07420202046564839 1102 48-49 -0.23725655069848273 1102 50-51 -0.22592199674294022 1102 52-53 -0.2778506968761363 1102 54-55 -0.38018886939486407 1102 56-57 -0.2801179159341487 1102 58-59 -0.3661314511798821 1102 60-61 -0.19017642762118925 1102 62-63 -0.11606493906570137 1102 64-65 -0.1251886658404402 1102 66-67 -0.2819626757649516 1102 68-69 -0.19629225102458747 1102 70-71 -0.12691464356654336 1102 72-73 -0.1303970946765105 1102 74-75 -0.12614289971406834 1102 76-77 -0.07541429855437798 1102 78-79 -0.1043608444204267 1102 80-81 -0.05276257011042418 1102 82-83 -0.048976263386698804 1102 84-85 0.08428678654164656 1102 86-87 0.07073470099580703 1102 88-89 -0.043999278334425185 1102 90-91 0.12186874278524229 1102 92-93 0.15768863985421788 1102 94-95 0.1114043550908086 1102 96-97 0.16181192569344205 1102 98-99 -0.10333642759725592 1102 100-101 0.6606713247434826 1103 1 -0.11297085362637205 1103 2 -0.18305465589775594 1103 3 -0.0854342740979348 1103 4 -0.11194259804258877 1103 5 -0.17445664127158267 1103 6 -0.15265682299584427 1103 7 -0.18260337511650704 1103 8 -0.09970032706541332 1103 9 -0.15654596126041298 1103 10-11 -0.16790621388566507 1103 12-13 -0.1683776062485478 1103 14-15 -0.2677357238855862 1103 16-17 -0.1753675532592851 1103 18-19 -0.23801165638161592 1103 20-21 -0.17891713083870542 1103 22-23 -0.19785430473417165 1103 24-25 -0.3584940342023728 1103 26-27 -0.38164388804944593 1103 28-29 -0.22888977623555462 1103 30-31 -0.2536377658346609 1103 32-33 -0.1905000831304875 1103 34-35 -0.17582877462145774 1103 36-37 -0.3958179068224368 1103 38-39 -0.2919886593440779 1103 40-41 -0.14680308158383326 1103 42-43 -0.04819502919019669 1103 44-45 -0.1543880547115677 1103 46-47 -0.08722608127123266 1103 48-49 -0.49447842327787583 1103 50-51 -0.5527583275911141 1103 52-53 -0.6218799740837255 1103 54-55 -0.4678825177294996 1103 56-57 -0.44295165673585757 1103 58-59 -0.43414135145098953 1103 60-61 -0.3044567819976436 1103 62-63 -0.4512756526598096 1103 64-65 -0.5120193687838963 1103 66-67 -0.26810774257362624 1103 68-69 -0.12768839500212437 1103 70-71 -0.20022082578963563 1103 72-73 -0.1550091928562196 1103 74-75 -0.19993834655760878 1103 76-77 -0.21690103364345248 1103 78-79 -0.21117847610129203 1103 80-81 -0.309933307426455 1103 82-83 -0.24524369173681748 1103 84-85 -0.2075361099533204 1103 86-87 -0.10658575068893228 1103 88-89 -0.19005308836540635 1103 90-91 -0.16930876011948115 1103 92-93 -0.26427501477397897 1103 94-95 -0.44790528859625667 1103 96-97 -0.3224943896628716 1103 98-99 0.0828343581308637 1103 100-101 0.4794122474778 1104 1 -0.02743000763879877 1104 2 0.024035047652795072 1104 3 0.0528694252611146 1104 4 0.035802559573639314 1104 5 0.08857010276822308 1104 6 0.034269357906374864 1104 7 0.08012481549692296 1104 8 0.13949856240986946 1104 9 0.12854905277982454 1104 10-11 0.18976402327624697 1104 12-13 0.13943685337562783 1104 14-15 0.1421175970511186 1104 16-17 0.06468556339164877 1104 18-19 0.16083221310586993 1104 20-21 0.2601312477458393 1104 22-23 0.16144592834907456 1104 24-25 0.23383537601355897 1104 26-27 0.2432804451126671 1104 28-29 0.24106622827330426 1104 30-31 0.18778346601558837 1104 32-33 0.19782359815079786 1104 34-35 0.26089464854041466 1104 36-37 0.059719083829548936 1104 38-39 0.2105322952276083 1104 40-41 0.24243271567723212 1104 42-43 0.33038423263057837 1104 44-45 0.30197701030608215 1104 46-47 0.40804143552827554 1104 48-49 0.4364370191042539 1104 50-51 0.25428653417512237 1104 52-53 0.2915803776686303 1104 54-55 0.31114269357933466 1104 56-57 0.34536162219179545 1104 58-59 0.3595597001858266 1104 60-61 0.28107025295543764 1104 62-63 0.5066576994016785 1104 64-65 0.5857962544934026 1104 66-67 0.5942406152062176 1104 68-69 0.48904834552320864 1104 70-71 0.518294717207354 1104 72-73 0.5981226796026959 1104 74-75 0.44002091382917996 1104 76-77 0.5604971202620135 1104 78-79 0.6175829150636396 1104 80-81 0.5761667973023279 1104 82-83 0.7329945256876549 1104 84-85 0.7932640484915687 1104 86-87 0.7971116689396602 1104 88-89 0.7796931059599608 1104 90-91 0.9898446058353194 1104 92-93 0.9261606381470848 1104 94-95 0.9002985129056782 1104 96-97 1.1512863898436692 1104 98-99 1.3499444667502445 1104 100-101 1.6090981551683683 1105 1 0.12904772705087453 1105 2 0.1350958112489664 1105 3 -0.007775912793171358 1105 4 0.06548768855694931 1105 5 0.13042293815292538 1105 6 0.13183230400877477 1105 7 0.19412212717379873 1105 8 0.0806071912714117 1105 9 0.09596250188902644 1105 10-11 0.04285758535122142 1105 12-13 0.08978132404761396 1105 14-15 0.0381751524568088 1105 16-17 0.08020372128145681 1105 18-19 0.0640397010692979 1105 20-21 0.040185201547899396 1105 22-23 0.147647196638097 1105 24-25 0.1358175459680382 1105 26-27 0.08109763445678908 1105 28-29 -0.036917037617776316 1105 30-31 -0.08730071971288567 1105 32-33 0.07254727225484459 1105 34-35 -0.030866858793935137 1105 36-37 -0.04273752363781114 1105 38-39 0.08300028357508893 1105 40-41 -0.0930889782473443 1105 42-43 0.1446586898141149 1105 44-45 -8.545105167172551E-4 1105 46-47 -0.16893581676195168 1105 48-49 -0.2248530013157648 1105 50-51 -0.17321655692811788 1105 52-53 -0.21088021076501917 1105 54-55 -0.1879820792714142 1105 56-57 -0.24052011500823056 1105 58-59 -0.11916964562432497 1105 60-61 -0.22862164367057147 1105 62-63 0.06777958253923799 1105 64-65 -0.22713696830957986 1105 66-67 -0.3301397590982802 1105 68-69 -0.20968441923446335 1105 70-71 -0.11019975159123874 1105 72-73 -0.03029099899750065 1105 74-75 -0.22240543829431658 1105 76-77 -0.1348664590482045 1105 78-79 -0.11529834442042741 1105 80-81 -0.13382449140672037 1105 82-83 -0.06718613992990896 1105 84-85 -0.12989627672995852 1105 86-87 -0.20148269869555335 1105 88-89 -0.3073191085813356 1105 90-91 0.0873876471062296 1105 92-93 0.05996969695298304 1105 94-95 0.14257719459697782 1105 96-97 0.20857600748356475 1105 98-99 0.27299941345212986 1105 100-101 0.499434827829905 1106 1 -0.03438632103230077 1106 2 -0.08120660790433476 1106 3 -0.02683236803383693 1106 4 0.054505907180434576 1106 5 0.11348824029717264 1106 6 0.07599372251230108 1106 7 -0.05519123814833904 1106 8 -0.0627769992619065 1106 9 -0.09582599713631623 1106 10-11 -0.33914402658459863 1106 12-13 -0.09852214473830401 1106 14-15 -0.08818718322570618 1106 16-17 -0.013884285416196462 1106 18-19 -0.1297006148201305 1106 20-21 -0.010264641007204034 1106 22-23 -0.02443195066035031 1106 24-25 -0.062082251602816996 1106 26-27 -0.2358023880353315 1106 28-29 0.05230642167148147 1106 30-31 -0.015593629694393485 1106 32-33 0.10594933153409869 1106 34-35 0.25497996981605553 1106 36-37 -0.05632995378725525 1106 38-39 0.11850566919012095 1106 40-41 0.05160100525842637 1106 42-43 -0.16577422116703389 1106 44-45 0.1325997180033056 1106 46-47 0.20162667386632904 1106 48-49 0.34182366185212487 1106 50-51 0.17669534890484329 1106 52-53 0.29265948784046714 1106 54-55 0.29405202129338903 1106 56-57 0.26252663113061914 1106 58-59 0.29640334133023316 1106 60-61 0.2973552019334633 1106 62-63 0.35018948907195124 1106 64-65 0.31978223496927427 1106 66-67 0.42890207625934096 1106 68-69 0.3298732980644772 1106 70-71 0.27980031088689117 1106 72-73 0.07882924641660338 1106 74-75 0.4393445117231778 1106 76-77 0.7104283127816586 1106 78-79 0.5587654207617554 1106 80-81 0.36905075225799777 1106 82-83 0.595127987625446 1106 84-85 0.38310542267524994 1106 86-87 0.43620629765572616 1106 88-89 0.7595337429206346 1106 90-91 0.7878574826637852 1106 92-93 0.9868368559473346 1106 94-95 1.2075835048883796 1106 96-97 1.303827044620565 1106 98-99 1.2719972642043658 1106 100-101 1.2445687184681802 1107 1 0.06021664106292235 1107 2 0.13655437765851275 1107 3 0.19162569469666835 1107 4 0.08069039125965105 1107 5 0.06734147433616755 1107 6 0.10250518276988885 1107 7 0.07370882171147741 1107 8 0.08084149420179187 1107 9 0.09841716243192167 1107 10-11 0.05753954672436379 1107 12-13 0.13194971793169685 1107 14-15 0.2248403715975158 1107 16-17 0.1408053768586015 1107 18-19 0.15163342224145282 1107 20-21 0.16940265429476398 1107 22-23 0.17521275238993184 1107 24-25 0.0768449704513472 1107 26-27 0.08305731472529487 1107 28-29 0.1501579049718771 1107 30-31 0.24391346015325155 1107 32-33 0.23270988584883412 1107 34-35 0.22572429117956716 1107 36-37 0.11872234309166885 1107 38-39 0.2628559971317799 1107 40-41 0.351727161421735 1107 42-43 0.14751755357983853 1107 44-45 0.12600580568183517 1107 46-47 0.1103195415375211 1107 48-49 -0.052720084084803887 1107 50-51 0.0832126664128694 1107 52-53 -0.017688857647200962 1107 54-55 0.05695341995330239 1107 56-57 0.11291604173675296 1107 58-59 0.0027887097899110813 1107 60-61 0.18348960973970208 1107 62-63 0.18720538088660277 1107 64-65 0.18466576579707805 1107 66-67 0.15343204800293364 1107 68-69 0.1148085786653894 1107 70-71 0.15947372090085565 1107 72-73 0.19833775230281958 1107 74-75 0.21927827973744485 1107 76-77 0.23127787155691237 1107 78-79 0.25836024063521634 1107 80-81 0.16624763954776256 1107 82-83 0.2358110975036034 1107 84-85 0.08984061702654245 1107 86-87 0.17513963442188185 1107 88-89 0.29949113303282715 1107 90-91 0.46870523920813767 1107 92-93 0.559127555792216 1107 94-95 0.6552338463467713 1107 96-97 0.7321449195328675 1107 98-99 1.0117725002644313 1107 100-101 1.2564669825336274 1108 1 0.047267339933995345 1108 2 0.0556789206464785 1108 3 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90-91 0.4372945188199573 2315 92-93 0.6899676159890618 2315 94-95 0.8274507502843989 2315 96-97 0.6254785854063947 2315 98-99 0.9055259096857924 2315 100-101 1.1119887062521627 2316 1 0.0826520375760893 2316 2 0.049343038702410524 2316 3 0.12496890829910257 2316 4 0.27459785804847314 2316 5 0.233381193017955 2316 6 0.25980625253357204 2316 7 0.2493925812436899 2316 8 0.3207541885511773 2316 9 0.16144429724370468 2316 10-11 -0.4074209771095454 2316 12-13 0.17014594007607542 2316 14-15 0.2633464749092056 2316 16-17 0.27548646871815663 2316 18-19 0.4274290084557819 2316 20-21 0.2827464068209693 2316 22-23 0.15782976621331812 2316 24-25 0.2823217832561795 2316 26-27 0.416043491330619 2316 28-29 0.3914610226458777 2316 30-31 0.33973814825112214 2316 32-33 0.4167981615622338 2316 34-35 0.12202862143205806 2316 36-37 -0.09009444977298386 2316 38-39 -0.06265126486601247 2316 40-41 -0.24529393744382588 2316 42-43 -0.0562737751367095 2316 44-45 -0.10001882313441968 2316 46-47 -0.07571155436396282 2316 48-49 -0.19316227103746542 2316 50-51 -0.25180255818361275 2316 52-53 -0.28396166085918395 2316 54-55 -0.41175666600504 2316 56-57 -0.32100112567992056 2316 58-59 -0.12059384524768291 2316 60-61 -0.2395143513500031 2316 62-63 -0.32402309584535516 2316 64-65 -0.36287404719637095 2316 66-67 -0.40104371390054183 2316 68-69 -0.43444770653306364 2316 70-71 -0.25489398678688957 2316 72-73 -0.4475448701002449 2316 74-75 -0.22595089759542475 2316 76-77 -0.3137087900798008 2316 78-79 -0.25650597153907384 2316 80-81 -0.3303248052799148 2316 82-83 -0.45798050067483587 2316 84-85 -0.24176591472953746 2316 86-87 -0.1674339960380884 2316 88-89 -0.4065919690123927 2316 90-91 -0.11749831229950303 2316 92-93 -0.2505542097220541 2316 94-95 0.09837469407385768 2316 96-97 -0.09192880523876212 2316 98-99 -0.3407900822582697 2316 100-101 -0.04429424729041642 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4086.0 3 688.0 4 346.0 5 326.0 6 363.0 7 409.0 8 458.0 9 583.0 10 899.0 11 1322.0 12 2478.0 13 3787.0 14 4104.0 15 6299.0 16 9525.0 17 15604.0 18 12430.0 19 5670.0 20 4662.0 21 4191.0 22 4289.0 23 4781.0 24 5373.0 25 6058.0 26 7172.0 27 8287.0 28 10011.0 29 12474.0 30 15771.0 31 20348.0 32 27729.0 33 39761.0 34 59926.0 35 97306.0 36 159262.0 37 280738.0 38 285595.0 39 39732.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.51710369107325 23.01916654741811 16.061487428903437 21.402242332605198 2 13.90551489187137 22.712226080316736 41.776010465979105 21.60624856183279 3 16.560887186076233 28.448863484578602 32.355250152313836 22.634999177031332 4 10.504907047050429 19.44428166139824 41.82546631597453 28.2253449755768 5 10.622888034934197 40.05393186383799 36.78216887447182 12.541011226755996 6 25.807502756401355 41.777734483766324 19.358945955568277 13.055816804264039 7 24.437674352847058 35.54220502687922 23.720349240175036 16.29977138009869 8 23.81552464972353 35.97743404976059 21.838202653955722 18.368838646560157 9 25.287014531726744 16.996604092327317 23.4120253055455 34.304356070400445 10-11 21.68611397016356 29.053030107115337 30.154052642195477 19.10680328052563 12-13 23.021049383035326 27.86103683167907 29.75589508583369 19.362018699451912 14-15 20.839763510266806 28.615576621492743 29.009941653281984 21.534718214958463 16-17 18.112883719045808 31.755528373076935 29.935193063123407 20.196394844753854 18-19 17.761377030669877 30.20132695212586 33.545298025517646 18.491997991686617 20-21 18.949871062048803 29.359671380601387 33.425569361033155 18.26488819631665 22-23 18.68524680871969 29.270144647241896 32.990611676065335 19.053996867973087 24-25 18.252894742171772 29.803207948728666 33.399664666241215 18.544232642858343 26-27 18.13128825419727 29.88657171287013 33.4778713353217 18.5042686976109 28-29 17.74510746193136 29.59960210618231 33.87984830729195 18.77544212459438 30-31 18.225430930078 29.51938915979302 33.77296232157896 18.48221758855002 32-33 18.08783770096771 29.841467004509504 33.339554031939336 18.731141262583446 34-35 18.42699389676218 29.72078549015551 33.24312092686459 18.609099686217718 36-37 18.544540345666714 29.265146889176275 33.10594559328162 19.084367171875396 38-39 17.665594071032636 30.398497935177993 33.0708512117228 18.86505678206658 40-41 17.915526168823558 30.05174470314881 33.47728886713229 18.555440260895345 42-43 17.970837369812763 29.798224868390662 32.88759683241227 19.343340929384308 44-45 18.29094368068698 29.9565421835624 32.62404958923463 19.128464546515982 46-47 18.586138528511704 30.039696564803243 32.152062411102975 19.222102495582085 48-49 18.19654006383662 30.394075823766105 32.00611210848346 19.40327200391382 50-51 18.40026069212725 30.235933271781022 31.799180854301206 19.564625181790518 52-53 18.32626812664173 30.93900929606744 31.442073855516274 19.29264872177456 54-55 18.757386808872706 30.383308031761878 31.412439752518733 19.446865406846683 56-57 18.771856113247797 30.828178360966568 30.910356269459076 19.489609256326556 58-59 18.674046003804566 30.322483352327446 31.482553066812585 19.520917577055403 60-61 18.903267113265787 31.03804996923671 31.01392189743157 19.04476102006594 62-63 18.646767772650712 30.70408079286487 31.48346754716802 19.165683887316394 64-65 18.663718465137098 30.372952950994907 31.399337257853745 19.563991326014246 66-67 18.711802217943376 30.60798912872542 30.719192202945393 19.961016450385806 68-69 18.6944736362473 30.262183322798432 31.199294053877512 19.844048987076757 70-71 18.755084313428103 30.48024356446565 31.45285548830091 19.311816633805343 72-73 18.982112248934207 29.92973130886425 31.444937663897797 19.643218778303744 74-75 18.54335397603195 30.75674478488906 30.93806500985024 19.761836229228752 76-77 18.454624903685545 30.449760485131804 31.34580810196318 19.749806509219468 78-79 18.40313462684522 30.300013918460227 31.091340864814693 20.205510589879857 80-81 18.495114506185075 30.47000363675849 30.85776654768204 20.17711530937439 82-83 18.67914154627839 30.464524668824524 31.054315191500947 19.802018593396138 84-85 18.596198675439616 30.063599581173488 30.81882306303511 20.52137868035178 86-87 18.333390070286313 30.31373524067169 31.148199363023565 20.20467532601843 88-89 18.878393686849428 29.812176531395334 31.118180686099233 20.191249095656 90-91 18.44391928113532 29.758574267802263 31.47954707250184 20.317959378560584 92-93 18.75879019359109 30.027301307153493 31.328465404555732 19.88544309469969 94-95 18.632159412209273 29.35312686086764 31.47745673307693 20.537256993846157 96-97 18.920288565416534 29.290742886593584 31.181216099574975 20.607752448414903 98-99 18.619102614931133 29.443486804681708 31.02425318463103 20.913157395756123 100-101 18.63738437914163 28.889229745776294 31.75818674093517 20.715199134146907 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2680.0 1 1660.0 2 733.0 3 820.5 4 664.5 5 682.0 6 1324.0 7 2613.5 8 4006.5 9 4333.5 10 3392.0 11 2576.0 12 2477.5 13 2442.5 14 2386.0 15 2347.0 16 2350.5 17 2422.5 18 2537.5 19 2823.5 20 3291.5 21 3850.5 22 4553.0 23 5311.0 24 6081.5 25 7671.5 26 11050.0 27 14711.5 28 16072.5 29 18272.5 30 22907.5 31 27227.5 32 33537.5 33 42129.0 34 50620.0 35 55121.5 36 59874.5 37 65765.5 38 63401.0 39 58268.0 40 53630.5 41 48799.5 42 44851.5 43 43896.5 44 43813.0 45 41519.5 46 37714.0 47 33559.0 48 32603.5 49 29826.0 50 24373.5 51 19778.0 52 16665.0 53 14505.0 54 12490.5 55 10692.5 56 9049.0 57 7471.5 58 5881.0 59 4603.5 60 3634.5 61 2818.0 62 2107.5 63 1518.5 64 1036.0 65 693.5 66 460.5 67 309.0 68 219.0 69 156.5 70 114.0 71 85.5 72 67.0 73 62.0 74 61.5 75 42.5 76 27.0 77 19.0 78 12.5 79 10.0 80 5.0 81 3.5 82 3.0 83 2.5 84 3.5 85 2.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24164726035643402 2 0.2167945705902385 3 0.2080230330256989 4 0.21326875647115887 5 0.21490267033122015 6 0.24293719235121924 7 0.24491508807655663 8 0.23192977266238524 9 0.22634006735164922 10-11 0.24027133289532976 12-13 0.24233522408698616 14-15 0.22638306508480874 16-17 0.24001334649637274 18-19 0.22999487467020738 20-21 0.22393219429471678 22-23 0.2219542985693794 24-25 0.21632159552548388 26-27 0.22388919656155726 28-29 0.23592856184621946 30-31 0.236057555045698 32-33 0.23618654824517651 34-35 0.24181925128907203 36-37 0.2212233371056678 38-39 0.22208329176885794 40-41 0.21842848445029978 42-43 0.2235882124294407 44-45 0.23747648023996173 46-47 0.2409592966258819 48-49 0.2459040359392253 50-51 0.24620502007134185 52-53 0.2631891246693474 54-55 0.24465710167759958 56-57 0.22053537337511567 58-59 0.20677609876407316 60-61 0.20449721890661923 62-63 0.21090388114738592 64-65 0.20385225290922662 66-67 0.2031642891786745 68-69 0.20841001262413444 70-71 0.2081090284920179 72-73 0.21060289701526935 74-75 0.21275278367324474 76-77 0.22272825776625058 78-79 0.21692356378971703 80-81 0.2130967655385208 82-83 0.2098289378183982 84-85 0.21215081540901162 86-87 0.2172675456549931 88-89 0.21331175420431836 90-91 0.22169631217042238 92-93 0.21223681087533067 94-95 0.2097429423520792 96-97 0.22019139150983963 98-99 0.22290024869888858 100-101 0.20449721890661923 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1162852.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.201214478037166 #Duplication Level Percentage of deduplicated Percentage of total 1 77.96166663094574 32.121153479268294 2 12.500950178087678 10.301086589332947 3 3.976997327274294 4.915713595788263 4 1.668966545060032 2.750537943187483 5 0.8275187451976982 1.7047388652743276 6 0.4757565007449639 1.1761047375908225 7 0.32237245303735235 0.9297495605582043 8 0.21927945572618154 0.7227663908801328 9 0.18142825351915132 0.6727557947056431 >10 1.3667747584653394 11.986038975824068 >50 0.2593744745100292 7.511979177824037 >100 0.22055267608097362 17.448660607654272 >500 0.01502345565050788 4.108682345185803 >1k 0.0027125683813417007 1.5158245545345683 >5k 4.1731821251410773E-4 1.1887083776967715 >10k+ 2.0865910625705386E-4 0.9454990046943729 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10998 0.9457781385765341 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7839 0.6741184604747638 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5988 0.5149408523182658 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2227 0.19151190349244787 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1738 0.14946012046244922 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1674 0.14395641061803222 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1670 0.14361242875275618 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1360 0.1169538341938613 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1356 0.11660985232858523 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1204 0.10353854144809485 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.599546631901566E-5 0.0 10-11 0.0 0.0 0.0 8.599546631901566E-5 0.0 12-13 0.0 0.0 0.0 8.599546631901566E-5 0.0 14-15 0.0 0.0 0.0 8.599546631901566E-5 0.0 16-17 0.0 0.0 0.0 8.599546631901566E-5 0.0 18-19 0.0 0.0 0.0 8.599546631901566E-5 0.0 20-21 0.0 0.0 0.0 2.1498866579753917E-4 0.0 22-23 0.0 0.0 0.0 5.159727979140939E-4 0.0 24-25 0.0 0.0 0.0 9.459501295091722E-4 0.0 26-27 0.0 0.0 0.0 0.0013759274611042506 0.0 28-29 0.0 0.0 0.0 0.0024508707900919463 0.0 30-31 0.0 0.0 0.0 0.005503709844417002 0.0 32-33 0.0 0.0 0.0 0.011824376618864653 0.0 34-35 0.0 0.0 0.0 0.02003694365233065 0.0 36-37 0.0 0.0 0.0 0.0323772930691094 0.0 38-39 0.0 0.0 0.0 0.055381080309446086 0.0 40-41 0.0 0.0 0.0 0.0801477746093226 0.0 42-43 0.0 0.0 0.0 0.10874126716039531 0.0 44-45 0.0 0.0 0.0 0.14481636528122238 0.0 46-47 0.0 0.0 0.0 0.1934038037514662 0.0 48-49 0.0 0.0 0.0 0.24530206767499219 0.0 50-51 0.0 0.0 0.0 0.30184408677974495 0.0 52-53 0.0 0.0 0.0 0.36922153464069374 0.0 54-55 0.0 0.0 0.0 0.4524651460375009 0.0 56-57 0.0 0.0 0.0 0.5512739368380499 0.0 58-59 0.0 0.0 0.0 0.6646159614465126 0.0 60-61 0.0 0.0 0.0 0.7935661631918766 0.0 62-63 0.0 0.0 0.0 0.9291810135769643 0.0 64-65 0.0 0.0 0.0 1.0868966988060391 0.0 66-67 0.0 0.0 0.0 1.277419654435818 0.0 68-69 0.0 0.0 0.0 1.4872055945210567 0.0 70-71 0.0 0.0 0.0 1.7360334763151286 0.0 72-73 0.0 0.0 0.0 1.992858936476869 0.0 74-75 0.0 0.0 0.0 2.268216419630357 0.0 76-77 0.0 0.0 0.0 2.5823578580937214 0.0 78-79 0.0 0.0 0.0 2.93893805918552 0.0 80-81 0.0 0.0 0.0 3.341439839291673 0.0 82-83 0.0 0.0 0.0 3.7503052839054325 0.0 84-85 0.0 0.0 0.0 4.177788746977259 0.0 86-87 0.0 0.0 0.0 4.658890383298992 0.0 88-89 0.0 0.0 0.0 5.179205952262197 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4635 0.0 40.0808 1 CATTCCG 305 0.0 35.82458 9 GTATCAA 7570 0.0 35.58742 1 ATCAACG 9290 0.0 28.842657 3 TCAACGC 9335 0.0 28.704857 4 CAACGCA 9500 0.0 28.207516 5 TATCAAC 9655 0.0 27.850695 2 AACGCAG 9705 0.0 27.708405 6 GCGCACT 35 0.008398281 27.139557 86-87 CGCCGCA 35 0.008398281 27.139557 86-87 GTACATG 5780 0.0 27.126606 1 TAGGCAT 480 0.0 26.725872 5 ACATGGG 5840 0.0 25.055885 3 TACATGG 6180 0.0 24.984276 2 CGCAGAG 10735 0.0 24.917068 8 ACGCAGA 10705 0.0 24.897058 7 CATGGGA 3635 0.0 24.310793 4 GTGTACG 100 0.001651092 23.756329 1 TTCGAGA 100 0.0016519212 23.754278 2 GAGTACT 6365 0.0 23.584305 12-13 >>END_MODULE