FastQCFastQC Report
Thu 26 May 2016
SRR937188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162852
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112460.9671050142236501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96230.8275343723878879No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75100.6458259520558076No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27210.23399366385404163No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22470.1932318128188282No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.16915308224950382No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19020.1635633769387678No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT14090.12116761204349308No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT13540.1164378613959472No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA12940.11127813341680627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67900.034.8623921
CGTCAGA702.0542427E-433.9260522
GTATCAA97750.028.5442711
GTGTAGC3500.027.1618631
CCACGCG951.1910743E-825.00244394-95
ACAGCGA950.001226172125.001378
TAATACC6150.023.9413134
CATTCCG3000.023.75139
CATATAG6200.023.7482363
GAGTACT83100.022.60803812-13
GACATAT7200.022.4462641
TATCAAC124300.022.3153172
ATCAACG124150.022.3040223
TCAACGC124300.022.2771074
AGGCTTA8150.022.1455966
CAACGCA125750.022.0202355
AGTACTT86800.021.72642112-13
AACGCAG128150.021.6078386
GTACTTT88450.021.13042614-15
GTATGGT3151.4551915E-1121.1258961
Produced by FastQC (version 0.11.5)