##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937188_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1162852 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.376243924420304 31.0 31.0 34.0 30.0 34.0 2 31.6322971452945 31.0 31.0 34.0 30.0 34.0 3 31.6228238847248 31.0 31.0 34.0 30.0 34.0 4 35.47318145387375 37.0 35.0 37.0 33.0 37.0 5 35.37331405888282 37.0 35.0 37.0 33.0 37.0 6 35.43827073436688 37.0 35.0 37.0 33.0 37.0 7 35.351969124187775 37.0 35.0 37.0 33.0 37.0 8 35.33448022620247 37.0 35.0 37.0 32.0 37.0 9 36.873839491182025 39.0 37.0 39.0 33.0 39.0 10-11 36.757968769886446 39.0 36.0 39.0 32.0 39.0 12-13 36.66099426238249 38.0 35.0 39.0 32.0 39.0 14-15 37.52548217657965 39.0 36.0 40.5 32.0 41.0 16-17 37.4777207245634 39.0 36.0 40.0 32.0 41.0 18-19 37.54828989415678 39.0 36.0 40.0 32.0 41.0 20-21 37.48292903998101 39.0 36.0 40.0 32.0 41.0 22-23 37.27449924839962 39.0 36.0 40.0 32.0 41.0 24-25 37.18057285019934 39.0 36.0 40.0 31.5 41.0 26-27 37.047656107570006 39.0 36.0 40.0 31.0 41.0 28-29 36.877115488471446 39.0 36.0 40.0 31.0 41.0 30-31 36.82262403126107 39.0 35.5 40.0 31.0 41.0 32-33 36.65636039667989 38.0 35.0 40.0 31.0 41.0 34-35 36.45220887954787 38.0 35.0 40.0 30.0 41.0 36-37 36.16165384760916 38.0 35.0 40.0 30.0 41.0 38-39 35.979805684644305 38.0 35.0 40.0 30.0 41.0 40-41 35.75371414419032 38.0 35.0 40.0 29.5 41.0 42-43 35.43488380292591 38.0 34.5 40.0 28.0 41.0 44-45 34.95327135353425 38.0 34.0 40.0 26.5 41.0 46-47 34.42784120421172 38.0 33.0 40.0 24.5 41.0 48-49 33.974642516846515 38.0 33.0 40.0 23.0 41.0 50-51 33.67690643349283 38.0 33.0 40.0 21.5 41.0 52-53 33.38864189079952 37.5 33.0 40.0 19.5 41.0 54-55 33.11717914231562 37.0 32.0 40.0 19.0 41.0 56-57 32.78731085297183 37.0 31.5 39.5 17.5 40.0 58-59 32.48122245995191 36.5 31.0 39.0 14.5 40.0 60-61 32.08345903003993 36.0 31.0 39.0 12.0 40.0 62-63 31.66356122705211 36.0 30.5 39.0 8.5 40.0 64-65 31.18720224069787 35.0 29.5 38.5 8.0 40.0 66-67 30.669158241977485 35.0 28.5 38.0 7.0 40.0 68-69 30.11305995947894 34.0 28.0 38.0 3.5 39.5 70-71 29.66673230987262 34.0 28.0 37.0 2.0 39.0 72-73 29.08132720242989 34.0 26.5 36.5 2.0 39.0 74-75 28.48366215133138 33.0 26.0 36.0 2.0 39.0 76-77 27.485202760110486 31.5 26.0 34.5 2.0 37.0 78-79 28.068998892378396 33.0 26.0 35.0 2.0 37.5 80-81 27.795537179279908 33.0 26.0 35.0 2.0 37.0 82-83 27.324042526478003 32.5 26.0 35.0 2.0 36.5 84-85 26.767991541485934 32.0 25.5 35.0 2.0 36.0 86-87 26.17297472077272 32.0 24.5 34.0 2.0 36.0 88-89 25.414415162032657 31.0 21.0 34.0 2.0 35.0 90-91 24.612532807270398 31.0 19.0 34.0 2.0 35.0 92-93 23.932978573369613 30.5 13.5 34.0 2.0 35.0 94-95 23.113404801298877 30.0 4.5 34.0 2.0 35.0 96-97 22.21966122945998 29.5 2.0 34.0 2.0 35.0 98-99 20.997561168575196 29.0 2.0 34.0 2.0 35.0 100-101 18.37533151252266 23.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.010615244719886618 1101 2 -0.016325476847185882 1101 3 -0.07742561557583727 1101 4 -0.012749429168202653 1101 5 -0.07978632742724301 1101 6 -0.0709172982827937 1101 7 -0.19535209518871 1101 8 -0.09139291672273231 1101 9 -0.06384272875594377 1101 10-11 0.024186695449536444 1101 12-13 0.0756099891892319 1101 14-15 -0.13033180091922958 1101 16-17 -0.06577044875858462 1101 18-19 0.0033541170082571625 1101 20-21 -0.06914623039111234 1101 22-23 -0.01683109753702894 1101 24-25 -0.0015582317908240384 1101 26-27 0.109755644936115 1101 28-29 -0.1483957524526076 1101 30-31 -0.1366959733766393 1101 32-33 0.12277200932938115 1101 34-35 -0.10360976233526742 1101 36-37 0.039781073003887 1101 38-39 -0.06404062531000676 1101 40-41 -0.2213687107279796 1101 42-43 -0.18016204015835768 1101 44-45 0.09626456553232998 1101 46-47 -0.15279032589737085 1101 48-49 -0.34865370574745924 1101 50-51 -0.30841072753795373 1101 52-53 -0.07739919486284919 1101 54-55 -0.1915051791452953 1101 56-57 -0.1900484721160609 1101 58-59 -0.30617793260437765 1101 60-61 -0.10045315646489428 1101 62-63 -0.21030741031648148 1101 64-65 -0.5382440184864414 1101 66-67 -0.44906835373644327 1101 68-69 -0.20813149054062663 1101 70-71 -0.0810505512795352 1101 72-73 0.15376634283672175 1101 74-75 -0.12905416522972502 1101 76-77 0.01267324413459292 1101 78-79 -0.040703397389069806 1101 80-81 -0.13110984846178297 1101 82-83 -0.30426573478256813 1101 84-85 -0.06333899314995506 1101 86-87 0.11396482631093363 1101 88-89 0.15175278822426463 1101 90-91 0.1102223838777796 1101 92-93 0.4705073319566502 1101 94-95 0.7329939053920675 1101 96-97 0.6920841223937337 1101 98-99 0.8070298607013981 1101 100-101 1.271912227525707 1102 1 -0.07412498360828579 1102 2 -0.07399769046947924 1102 3 -0.005303905049522939 1102 4 -0.10294147638182949 1102 5 -0.1247505302136176 1102 6 -0.2144036566419274 1102 7 -0.18993655726301029 1102 8 -0.13053523483419127 1102 9 -0.27917748107793017 1102 10-11 -0.1494092314080504 1102 12-13 -0.2451439291544233 1102 14-15 0.026891738167456936 1102 16-17 -0.07947376725704203 1102 18-19 -0.21019682145923468 1102 20-21 0.04920709778225785 1102 22-23 -0.1753450294255714 1102 24-25 -0.04496960949980178 1102 26-27 -0.08330259809794427 1102 28-29 -0.03411677605941321 1102 30-31 0.10103743413110067 1102 32-33 -0.010144496088578592 1102 34-35 -0.17971028478109474 1102 36-37 -0.12268748349765701 1102 38-39 -0.07959911021712429 1102 40-41 -0.03166887520166739 1102 42-43 -0.006561275839473524 1102 44-45 -0.3194571836936788 1102 46-47 0.11031798486114042 1102 48-49 0.23044362397389762 1102 50-51 0.3463916621679246 1102 52-53 0.11641369213405284 1102 54-55 0.48302195901259637 1102 56-57 0.44574995087155145 1102 58-59 0.6431750569467098 1102 60-61 0.3534917932255084 1102 62-63 0.6163179766804205 1102 64-65 0.7878915755538038 1102 66-67 0.6211050692511719 1102 68-69 0.6010306611621594 1102 70-71 0.8118432180703081 1102 72-73 0.8645502534404379 1102 74-75 0.8023312836866836 1102 76-77 0.9837875533451204 1102 78-79 0.9936534621310535 1102 80-81 0.77487650989735 1102 82-83 0.6713849037623234 1102 84-85 0.7452390351008162 1102 86-87 0.998087068957993 1102 88-89 1.1438834480539875 1102 90-91 1.067541462825151 1102 92-93 1.1172517205015389 1102 94-95 1.273158137203211 1102 96-97 1.2001469610160207 1102 98-99 1.2469582662741558 1102 100-101 2.114628220172765 1103 1 -0.07739582501029929 1103 2 -0.010439886441591995 1103 3 -0.03751367460800381 1103 4 -0.028657370068891908 1103 5 -0.055551508137710925 1103 6 -0.22787976527864373 1103 7 -0.022493078370679598 1103 8 -0.10669772999298033 1103 9 0.001828037899606727 1103 10-11 -0.145623709041665 1103 12-13 -0.05626872340306477 1103 14-15 -0.1327365175451689 1103 16-17 -0.19751846371327986 1103 18-19 -0.2010962574584596 1103 20-21 -0.34264272793423345 1103 22-23 -0.2668245027021001 1103 24-25 -0.09667613544867493 1103 26-27 -0.0872566550305578 1103 28-29 -0.16409565628791967 1103 30-31 -0.23724472359546667 1103 32-33 -0.06949710549988453 1103 34-35 0.05350948187266624 1103 36-37 0.04807827463557146 1103 38-39 -0.09740719261449016 1103 40-41 0.19228451606479524 1103 42-43 0.08650520605440448 1103 44-45 -0.13376537501822838 1103 46-47 0.18746778395099284 1103 48-49 0.11037318439218069 1103 50-51 0.19766641284569886 1103 52-53 0.37519443380717377 1103 54-55 0.32740101352460016 1103 56-57 0.2317706591403379 1103 58-59 0.1900570161837365 1103 60-61 0.3309662558513793 1103 62-63 0.21991983570443097 1103 64-65 0.1957769962548106 1103 66-67 0.5231961686895907 1103 68-69 0.33245252405913917 1103 70-71 0.5738941959943986 1103 72-73 0.5737446158532933 1103 74-75 0.8154122286905583 1103 76-77 0.8189943334574359 1103 78-79 0.6176994415075043 1103 80-81 0.7133042064504131 1103 82-83 0.770859027503608 1103 84-85 0.6029713525872609 1103 86-87 0.7268731964754203 1103 88-89 0.7993403976085922 1103 90-91 0.8013125220815418 1103 92-93 1.1954190810747356 1103 94-95 1.444322992451081 1103 96-97 1.715633078851031 1103 98-99 2.130655497102971 1103 100-101 2.3305022398280713 1104 1 0.118490484904612 1104 2 -0.025851825522590133 1104 3 0.030552980382950068 1104 4 -0.07043839406012609 1104 5 -0.05586064022879356 1104 6 -0.0742540454886651 1104 7 0.05033397574153753 1104 8 -0.028630122922201906 1104 9 -0.0405536191659408 1104 10-11 -0.08593770731852146 1104 12-13 0.06964706572418322 1104 14-15 0.09593278046639142 1104 16-17 -0.26398450796265394 1104 18-19 -0.11722155116332544 1104 20-21 -0.09632116473670749 1104 22-23 -0.21085337540433358 1104 24-25 0.07448695347440548 1104 26-27 -0.345164794607804 1104 28-29 -0.10743750348733272 1104 30-31 -0.24707582933627492 1104 32-33 -0.08344566262878317 1104 34-35 0.0406508210610923 1104 36-37 0.10578724146440521 1104 38-39 -0.05646687861621302 1104 40-41 -0.03675885528512168 1104 42-43 0.27472962419846425 1104 44-45 0.3938252518146683 1104 46-47 0.4438684638065098 1104 48-49 0.514247208951133 1104 50-51 0.18325445143197072 1104 52-53 0.361327861329805 1104 54-55 0.38997792923262864 1104 56-57 0.43037568588673025 1104 58-59 0.28439696039890805 1104 60-61 0.4689520075654201 1104 62-63 0.7589583408685883 1104 64-65 0.4639188185355927 1104 66-67 0.39574882399320543 1104 68-69 0.2875111239846184 1104 70-71 0.5400606877212972 1104 72-73 0.5934547251779172 1104 74-75 0.5424488861146024 1104 76-77 0.38408926521917053 1104 78-79 0.44909198166823217 1104 80-81 0.4826214359216294 1104 82-83 0.4042792038381968 1104 84-85 0.3051184689020303 1104 86-87 0.35191448265298675 1104 88-89 0.5501399683498889 1104 90-91 0.47393135280997356 1104 92-93 0.830987020198048 1104 94-95 0.7569148946007829 1104 96-97 0.9880292874576 1104 98-99 1.3640722647567038 1104 100-101 2.0677146911515187 1105 1 0.02873959972504636 1105 2 3.4644533299044156E-4 1105 3 0.07604254556776269 1105 4 0.05140844645767828 1105 5 0.019636043860458585 1105 6 -0.14379215972834203 1105 7 -0.148009473929676 1105 8 -0.09985429347616304 1105 9 -0.05467902428780036 1105 10-11 -0.005596538506814852 1105 12-13 0.07524090880853862 1105 14-15 0.06713575822919182 1105 16-17 0.062197721940492556 1105 18-19 -0.01378960695305409 1105 20-21 0.08273333235015912 1105 22-23 -0.0064407084379212165 1105 24-25 -0.1109804119689386 1105 26-27 -0.09944843143127002 1105 28-29 0.2023560248665106 1105 30-31 0.0027590776496211333 1105 32-33 -0.01698284485400592 1105 34-35 -0.056822553299610945 1105 36-37 0.13519253193444314 1105 38-39 0.015284184535964584 1105 40-41 0.10879698127981641 1105 42-43 0.21638914854324298 1105 44-45 0.1289610261828713 1105 46-47 0.030779983317927417 1105 48-49 0.15951384002327984 1105 50-51 0.25842387667410804 1105 52-53 0.10262125386244492 1105 54-55 0.3014488494446965 1105 56-57 0.4459283845135289 1105 58-59 0.44995071281091015 1105 60-61 0.3317614691514308 1105 62-63 0.5545895816186857 1105 64-65 0.3167061974673864 1105 66-67 0.49400726832524455 1105 68-69 0.3774581302979634 1105 70-71 0.6796480020209259 1105 72-73 0.7349640265885853 1105 74-75 0.864985604674338 1105 76-77 0.9742148295796902 1105 78-79 0.7220340562668568 1105 80-81 0.6196199512553768 1105 82-83 0.672629116725286 1105 84-85 0.957242314421805 1105 86-87 0.5533484501308337 1105 88-89 0.7168435174984289 1105 90-91 0.9356163085041622 1105 92-93 1.3528805785262286 1105 94-95 1.538701154178522 1105 96-97 1.6140696076209586 1105 98-99 1.9179845008420564 1105 100-101 2.51955202418511 1106 1 -0.32013017085525064 1106 2 -0.06887370261522818 1106 3 -0.07823533217502998 1106 4 -0.0853143255721136 1106 5 -0.24629025693426598 1106 6 -0.13115783174313833 1106 7 -0.05755738216179651 1106 8 -0.030044976737023887 1106 9 -0.09662434342610737 1106 10-11 -0.22467017523396038 1106 12-13 -0.29650241852689163 1106 14-15 -0.3250329202333475 1106 16-17 -0.14224103699388024 1106 18-19 0.06660131872295949 1106 20-21 -0.1944796431084228 1106 22-23 -0.13040575817051092 1106 24-25 -0.21163205889251202 1106 26-27 -0.27003716287526913 1106 28-29 -0.25674297798249057 1106 30-31 -0.1298788740672805 1106 32-33 -0.148489005197888 1106 34-35 -0.08058642344505529 1106 36-37 0.007850851522583469 1106 38-39 -0.2594621542454618 1106 40-41 -0.10598409534336639 1106 42-43 -0.2965647550904791 1106 44-45 0.14993805922130576 1106 46-47 0.10152022423361018 1106 48-49 0.23669994988483722 1106 50-51 0.30794435700598655 1106 52-53 0.51531930954296 1106 54-55 0.6257012985874937 1106 56-57 0.3341656264788426 1106 58-59 0.30996876899124715 1106 60-61 0.2714984986910878 1106 62-63 0.5505285029962153 1106 64-65 0.6041887035902427 1106 66-67 0.7679595100406473 1106 68-69 0.8659218554941432 1106 70-71 0.9451997464103918 1106 72-73 0.828212639574037 1106 74-75 0.7850741986664396 1106 76-77 0.798705632798562 1106 78-79 0.8555635709367237 1106 80-81 0.7558513327718437 1106 82-83 0.4125001618594908 1106 84-85 0.6040937289267276 1106 86-87 0.7124168386948355 1106 88-89 0.9420600042280753 1106 90-91 0.9440280518939765 1106 92-93 1.0102489371007302 1106 94-95 1.238316601272036 1106 96-97 1.3924056275139982 1106 98-99 1.660584278419904 1106 100-101 1.9212751710836962 1107 1 -0.03641010880701501 1107 2 -0.15993270636773005 1107 3 -0.01868576117034948 1107 4 -0.005009491087712092 1107 5 0.07662317805347385 1107 6 -0.05160929058469321 1107 7 -0.08766236211197764 1107 8 -0.03558168753689017 1107 9 9.844823560953841E-4 1107 10-11 -0.0021709315272886442 1107 12-13 0.11600409846879245 1107 14-15 -0.016701142396932767 1107 16-17 0.04754642849017898 1107 18-19 -0.08658605536225394 1107 20-21 0.07723273371230732 1107 22-23 0.1411891518144941 1107 24-25 -0.07517131856180015 1107 26-27 0.013445712712865543 1107 28-29 -0.02369907435035401 1107 30-31 0.04137489935367711 1107 32-33 -0.11249073019032352 1107 34-35 -0.10910093462210568 1107 36-37 -0.12063437340226812 1107 38-39 -0.15751606669565632 1107 40-41 -0.1741550884369616 1107 42-43 -0.3311222108553764 1107 44-45 0.015235781330176223 1107 46-47 -0.32982572042502767 1107 48-49 -0.298950752019735 1107 50-51 -0.047878136123010506 1107 52-53 -0.05065457098200454 1107 54-55 -0.0234857665438426 1107 56-57 -0.17229891140984677 1107 58-59 -0.16330285938874312 1107 60-61 0.06069444624617404 1107 62-63 0.22323371594909958 1107 64-65 0.20270862146000468 1107 66-67 0.14280680315419403 1107 68-69 0.11650661505722937 1107 70-71 0.306981830947894 1107 72-73 0.4761243045135686 1107 74-75 0.543368644576983 1107 76-77 0.6069958949587928 1107 78-79 0.49779756487350113 1107 80-81 0.5022053552868577 1107 82-83 0.42826710765739406 1107 84-85 0.5344561704346802 1107 86-87 0.5964767499993258 1107 88-89 0.8673414418934122 1107 90-91 0.9645232792003462 1107 92-93 1.2775632268608383 1107 94-95 1.1825797141110037 1107 96-97 1.6679648018347812 1107 98-99 2.0432967002964126 1107 100-101 2.4171824977959773 1108 1 -0.04453852856096674 1108 2 0.04231737262200497 1108 3 -0.08244802965520037 1108 4 0.02676820421753945 1108 5 -0.035707746301945065 1108 6 -0.0664418072728381 1108 7 -0.1386771921210581 1108 8 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88-89 -0.6711390625392362 2316 90-91 -0.7998579015816283 2316 92-93 -0.37784891509167906 2316 94-95 -0.34455240305102564 2316 96-97 -0.602839002967027 2316 98-99 -0.2589209710139819 2316 100-101 -0.9669461972001159 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 16.0 5 57.0 6 98.0 7 293.0 8 636.0 9 1182.0 10 1915.0 11 3049.0 12 4656.0 13 6563.0 14 8375.0 15 11632.0 16 15974.0 17 20683.0 18 16779.0 19 10987.0 20 10134.0 21 10614.0 22 11649.0 23 13451.0 24 15504.0 25 18522.0 26 22347.0 27 27234.0 28 32682.0 29 39071.0 30 47123.0 31 55875.0 32 67879.0 33 82635.0 34 103933.0 35 132816.0 36 158542.0 37 152050.0 38 56130.0 39 1754.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.982749155441745 25.443188721554776 17.40056757068389 19.17349455231959 2 15.744307960084344 25.381217902192198 39.11030810455673 19.76416603316673 3 17.859366454200533 29.102413720748643 33.246191260796735 19.792028564254093 4 12.104807834530963 22.005809853704513 40.46671459480656 25.422667716957964 5 10.654922843009777 40.826212737267205 36.57810640303262 11.940758016690403 6 25.799843832233165 43.27653046131408 18.211861870642178 12.711763835810578 7 24.07554873707058 36.442986725739814 23.427142921025204 16.054321616164398 8 24.37386700973125 37.093370437510536 21.07361899880638 17.459143553951836 9 25.25428859390533 17.304523705510245 23.145937746162023 34.2952499544224 10-11 22.1980288102259 29.712104130279805 29.56444978763401 18.52541727186028 12-13 23.64952549675566 28.337798150146842 29.48013192351188 18.53254442958561 14-15 21.134976764025 29.24232834444968 28.935754507022388 20.68694038450293 16-17 18.20915301345313 32.25492152053744 29.984125236917507 19.551800229091924 18-19 17.853131782892405 30.28476538716879 34.01198088836757 17.85012194157124 20-21 19.057283300024423 29.15237708667999 34.34882512993915 17.44151448335644 22-23 18.89884580341207 29.093123881244555 33.8626392975495 18.145391017793884 24-25 18.58889189874833 29.62905632306971 34.23371128568296 17.548340492499 26-27 18.443436384744377 29.545513178827882 34.475469751042695 17.535580685385046 28-29 18.057907351333778 29.337654999944103 34.78431369176565 17.820123956956476 30-31 18.523283312551058 29.16425162316722 34.674549597970504 17.63791546631122 32-33 18.599443437341982 29.609657978831354 33.965715327487935 17.825183256338725 34-35 18.721900981380262 29.28545507080867 34.33330294826857 17.659340999542504 36-37 19.031871639727154 28.96017721945699 33.864799647762574 18.143151493053285 38-39 18.124115050240853 30.057784827516226 33.96482860836988 17.853271513873047 40-41 18.42592043176641 29.6740941014799 34.22003334907052 17.679952117683175 42-43 18.52512501983503 29.404957618609195 33.714266301601725 18.35565105995405 44-45 18.929018759521334 29.43859029048901 33.308652581438956 18.3237383685507 46-47 19.200594744645063 29.866784423125214 32.55362677279654 18.378994059433186 48-49 18.88899017243811 30.142141906278702 32.45967672584301 18.509191195440177 50-51 19.04696384406614 29.999647418588093 32.00114889943002 18.952239837915748 52-53 19.118383078844083 30.67041205587641 31.549285721656755 18.66191914362275 54-55 19.47530726180116 30.191460306212658 31.53664438810786 18.796588043878327 56-57 19.604859431810755 30.52060795354869 31.109891886499746 18.76464072814081 58-59 19.380282271518645 30.090974603818886 31.641730847949695 18.887012276712774 60-61 19.642396452859003 30.94392063650404 30.9772008819695 18.43648202866745 62-63 19.407456838875454 30.465183875506085 31.60019503771761 18.527164247900853 64-65 19.51362663246339 30.172898259150028 31.40869663925273 18.90477846913385 66-67 19.488722554546925 30.505902728808138 30.654631887806875 19.350742828838065 68-69 19.46627773783766 30.092135542614194 31.16522996907603 19.276356750472114 70-71 19.609245200593026 30.34926198690805 31.472663761166515 18.568829051332415 72-73 19.962041601166785 29.72377396263669 31.343670561687986 18.970513874508534 74-75 19.442456993667292 30.63468093962086 30.743895181846014 19.17896688486583 76-77 19.332855771843708 30.323420349279186 31.28239879193569 19.061325086941416 78-79 19.187050458699815 30.25316205329655 31.031893998548398 19.527893489455238 80-81 19.2620633520818 30.361512507411742 30.805593668317638 19.570830472188817 82-83 19.419210671037543 30.392007213321428 30.883859763331166 19.304922352309866 84-85 19.035821212552495 30.15033394404841 30.826305666836795 19.9875391765623 86-87 18.903093428914428 30.310607024797655 30.94688748009205 19.83941206619587 88-89 19.356633518280915 29.728374720084755 31.14050627250931 19.774485489125013 90-91 19.161387227261216 29.68500527406429 31.325963833636273 19.827643665038224 92-93 19.351551377404014 30.173891693609033 31.003253723808854 19.471303205178096 94-95 19.12835855293709 29.629952908882647 31.115825573675753 20.125862964504513 96-97 19.543587662058457 29.524049492110777 30.767887916949014 20.16447492888175 98-99 19.212099921701025 29.58011369475268 30.72828364437217 20.479502739174123 100-101 19.06765175234564 29.326466802810426 31.298272229898043 20.307609214945884 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1970.0 1 1232.5 2 575.5 3 668.0 4 573.5 5 597.0 6 1157.5 7 2523.0 8 3765.0 9 4129.5 10 3549.5 11 2659.5 12 2394.5 13 2364.0 14 2344.5 15 2316.0 16 2348.5 17 2510.0 18 2726.0 19 3015.0 20 3468.0 21 4041.0 22 4760.0 23 5526.5 24 6304.0 25 8099.5 26 11781.5 27 15343.5 28 16800.5 29 19161.5 30 23797.0 31 28398.5 32 34570.5 33 42886.5 34 50528.5 35 54698.5 36 59602.5 37 64927.0 38 63485.5 39 59003.5 40 54795.0 41 50373.5 42 45518.0 43 43332.0 44 42819.0 45 40685.0 46 36903.0 47 33303.5 48 32323.0 49 29147.5 50 23880.5 51 19562.0 52 16287.0 53 14055.0 54 12013.5 55 10010.5 56 8373.5 57 6954.5 58 5554.0 59 4398.5 60 3474.5 61 2652.5 62 1943.0 63 1420.0 64 1006.5 65 692.5 66 495.0 67 366.0 68 257.0 69 178.5 70 121.5 71 80.5 72 54.5 73 48.0 74 41.5 75 33.0 76 30.5 77 19.0 78 7.5 79 6.0 80 4.5 81 4.5 82 3.0 83 2.5 84 2.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06071279922122506 2 0.0 3 0.0 4 0.0 5 0.0024078730569324384 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.599546631901566E-5 12-13 0.0049017415801838925 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.309614637116331E-4 24-25 6.019682642331096E-4 26-27 1.7199093263803132E-4 28-29 0.0024938685232514545 30-31 1.7199093263803132E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.009841321165548E-4 40-41 8.599546631901566E-5 42-43 0.011738381152545638 44-45 2.1498866579753917E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.299773315950783E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.2899319947852348E-4 82-83 3.009841321165548E-4 84-85 6.449659973926174E-4 86-87 0.0 88-89 0.0 90-91 0.007438607836594855 92-93 0.0026228617227299776 94-95 0.0 96-97 0.0 98-99 1.2899319947852348E-4 100-101 0.00950249902825123 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1162852.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.37674407800723 #Duplication Level Percentage of deduplicated Percentage of total 1 79.06326232030501 33.50443633319918 2 12.03013125010467 10.195955864210454 3 3.6602271931659933 4.653255330964741 4 1.545227332048168 2.6192681277018846 5 0.7873729234721785 1.6683150435966438 6 0.4584887403341037 1.1657556007071734 7 0.29485568084255803 0.8746516608908163 8 0.21135485112843594 0.7165224348732037 9 0.16374741758152428 0.6245174167458153 >10 1.3096583439091716 11.90160763140167 >50 0.25194123569895466 7.516750756747484 >100 0.20669413692414756 16.73669935539099 >500 0.014198812070945768 3.9174930442814637 >1k 0.002231241896862906 1.4653959718905187 >5k 4.056803448841648E-4 1.4727023220553883 >10k+ 2.028401724420824E-4 0.9666731053426075 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11246 0.9671050142236501 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9623 0.8275343723878879 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7510 0.6458259520558076 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2721 0.23399366385404163 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2247 0.1932318128188282 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1967 0.16915308224950382 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1902 0.1635633769387678 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1409 0.12116761204349308 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1354 0.1164378613959472 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1294 0.11127813341680627 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.599546631901566E-5 0.0 10-11 0.0 0.0 0.0 8.599546631901566E-5 0.0 12-13 0.0 0.0 0.0 8.599546631901566E-5 0.0 14-15 0.0 0.0 0.0 8.599546631901566E-5 0.0 16-17 0.0 0.0 0.0 8.599546631901566E-5 0.0 18-19 0.0 0.0 0.0 8.599546631901566E-5 0.0 20-21 0.0 0.0 0.0 2.1498866579753917E-4 0.0 22-23 0.0 0.0 0.0 4.2997733159507833E-4 0.0 24-25 0.0 0.0 0.0 8.599546631901567E-4 0.0 26-27 0.0 0.0 0.0 0.0012039365284662192 0.0 28-29 0.0 0.0 0.0 0.002235882124294407 0.0 30-31 0.0 0.0 0.0 0.00515972797914094 0.0 32-33 0.0 0.0 0.0 0.011394399287269576 0.0 34-35 0.0 0.0 0.0 0.01943497538809754 0.0 36-37 0.0 0.0 0.0 0.031990313470673826 0.0 38-39 0.0 0.0 0.0 0.05477911204521298 0.0 40-41 0.0 0.0 0.0 0.07885784261453736 0.0 42-43 0.0 0.0 0.0 0.10697836010085549 0.0 44-45 0.0 0.0 0.0 0.14223650129165188 0.0 46-47 0.0 0.0 0.0 0.18966300096658906 0.0 48-49 0.0 0.0 0.0 0.23997034876321321 0.0 50-51 0.0 0.0 0.0 0.2948784540079047 0.0 52-53 0.0 0.0 0.0 0.36096596987406826 0.0 54-55 0.0 0.0 0.0 0.44175871048078347 0.0 56-57 0.0 0.0 0.0 0.5379446395586025 0.0 58-59 0.0 0.0 0.0 0.6473308727163904 0.0 60-61 0.0 0.0 0.0 0.7706053736846994 0.0 62-63 0.0 0.0 0.0 0.9011464915569651 0.0 64-65 0.0 0.0 0.0 1.051681555348402 0.0 66-67 0.0 0.0 0.0 1.2348089008747458 0.0 68-69 0.0 0.0 0.0 1.435135339664893 0.0 70-71 0.0 0.0 0.0 1.672654817638014 0.0 72-73 0.0 0.0 0.0 1.9171399283829755 0.0 74-75 0.0 0.0 0.0 2.183166903440851 0.0 76-77 0.0 0.0 0.0 2.485268976619553 0.0 78-79 0.0 0.0 0.0 2.8234461479190816 0.0 80-81 0.0 0.0 0.0 3.206168970771861 0.0 82-83 0.0 0.0 0.0 3.594567494401695 0.0 84-85 0.0 0.0 0.0 3.9968112881088906 0.0 86-87 0.0 0.0 0.0 4.44768551801949 0.0 88-89 0.0 0.0 0.0 4.933258918589812 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6790 0.0 34.862392 1 CGTCAGA 70 2.0542427E-4 33.926052 2 GTATCAA 9775 0.0 28.544271 1 GTGTAGC 350 0.0 27.161863 1 CCACGCG 95 1.1910743E-8 25.002443 94-95 ACAGCGA 95 0.0012261721 25.00137 8 TAATACC 615 0.0 23.941313 4 CATTCCG 300 0.0 23.7513 9 CATATAG 620 0.0 23.748236 3 GAGTACT 8310 0.0 22.608038 12-13 GACATAT 720 0.0 22.446264 1 TATCAAC 12430 0.0 22.315317 2 ATCAACG 12415 0.0 22.304022 3 TCAACGC 12430 0.0 22.277107 4 AGGCTTA 815 0.0 22.145596 6 CAACGCA 12575 0.0 22.020235 5 AGTACTT 8680 0.0 21.726421 12-13 AACGCAG 12815 0.0 21.607838 6 GTACTTT 8845 0.0 21.130426 14-15 GTATGGT 315 1.4551915E-11 21.125896 1 >>END_MODULE