FastQCFastQC Report
Thu 26 May 2016
SRR937187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429648
Sequences flagged as poor quality0
Sequence length101
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140633.2731445276133018No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106042.468066882657431No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86162.005362529326332No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49741.1576918780024579No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26670.6207406993631996No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23130.5383476706513238No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20850.4852809741928276No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18890.439662235132015No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14410.3353908315644435No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13580.3160726920642014No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11450.26649722563586936No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11370.2646352362864484No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA8070.18782817562283544No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7990.18596618627341452No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7820.18200945890589507No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7790.1813112128998622No Hit
GTACATGGGAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG6040.14058019588127957No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6000.1396492012065691No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5170.12033106170632704No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA4840.11265035563996573No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC4800.11171936096525528No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4610.1072971362603806No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4540.1056678955796373No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT4530.10543514691095966No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4430.10310766022418351No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4410.10264216288682827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4370.10171116821211784No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.10147841954344021No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAGC200.002070984671.269093
CGTCACT900.058.1185951
ATGGGCG504.2278225E-757.0152665
ACTGGGC652.584944E-643.847668
GTCACTA1201.8189894E-1243.5584142
GAGGCGC1051.024091E-940.7156879
TAGGACA2750.039.7379154
CATTCCG608.280364E-539.5846949
GGACAAT1850.038.5103456
CCTTTAG756.8608297E-638.041261
TAGGCAT756.899436E-638.0101785
CATGGAT1152.4883775E-937.1838724
GGAGGCG1152.4938345E-937.1751948
GCGTCAA455.686379E-736.95218326-27
GACAATG1950.036.539727
GGTATCA54500.035.6854931
GTCGGAT401.0770338E-535.6262376-77
GCACTGG958.688585E-734.997126
GTATCAA84100.033.3596081
TTCGCGA501.2941946E-633.2647348-49
Produced by FastQC (version 0.11.5)