##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937187_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 429648 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00778544296727 33.0 31.0 34.0 31.0 34.0 2 32.04816268200946 33.0 31.0 34.0 30.0 34.0 3 31.995708114549586 33.0 31.0 34.0 30.0 34.0 4 35.71835781849328 37.0 35.0 37.0 35.0 37.0 5 35.77513918370387 37.0 35.0 37.0 35.0 37.0 6 35.90436822701374 37.0 35.0 37.0 35.0 37.0 7 35.86552945667151 37.0 35.0 37.0 35.0 37.0 8 35.87348480616691 37.0 35.0 37.0 35.0 37.0 9 37.46970077831155 39.0 37.0 39.0 35.0 39.0 10-11 37.3971600007448 39.0 37.0 39.0 35.0 39.0 12-13 37.26749804491118 39.0 37.0 39.0 34.5 39.0 14-15 38.19124376233568 40.0 38.0 41.0 33.5 41.0 16-17 38.23179323539269 40.0 38.0 41.0 34.0 41.0 18-19 38.390239219081664 40.0 38.0 41.0 34.5 41.0 20-21 38.433532798942394 40.0 38.0 41.0 35.0 41.0 22-23 38.41574032696533 40.0 38.0 41.0 34.5 41.0 24-25 38.34757289688303 40.0 38.0 41.0 34.0 41.0 26-27 38.201635990392134 40.0 38.0 41.0 34.0 41.0 28-29 37.99130800096823 40.0 37.0 41.0 34.0 41.0 30-31 37.48794827393587 39.0 36.0 41.0 34.0 41.0 32-33 36.92978321788999 38.5 35.0 40.5 33.0 41.0 34-35 36.658115480579454 38.0 35.0 41.0 33.0 41.0 36-37 36.38046377499721 38.0 35.0 40.5 32.5 41.0 38-39 36.10307042043719 38.0 35.0 40.0 31.5 41.0 40-41 35.73162798383793 38.0 35.0 40.0 31.0 41.0 42-43 35.21451164674338 38.0 35.0 40.0 29.5 41.0 44-45 34.568131354038655 38.0 35.0 40.0 25.0 41.0 46-47 33.678639258183445 38.0 34.5 40.0 16.0 41.0 48-49 32.96655634379771 37.5 33.0 40.0 2.0 41.0 50-51 31.858931031914498 36.0 32.0 39.0 2.0 40.5 52-53 31.940753826388114 36.5 33.0 39.5 2.0 40.0 54-55 32.17406691058727 37.0 33.0 40.0 2.0 41.0 56-57 31.885299128588983 36.0 33.0 40.0 2.0 41.0 58-59 31.63004599113693 36.0 33.0 40.0 2.0 41.0 60-61 31.313504310505344 35.5 32.5 40.0 2.0 41.0 62-63 30.93352581648233 35.0 31.5 39.0 2.0 41.0 64-65 30.500768070606636 35.0 31.0 39.0 2.0 41.0 66-67 30.064050106133394 35.0 30.5 39.0 2.0 40.0 68-69 29.573819731501136 35.0 29.0 38.0 2.0 40.0 70-71 29.10038682828734 35.0 29.0 37.5 2.0 40.0 72-73 28.602205759133057 35.0 27.5 37.0 2.0 39.5 74-75 28.054052387070346 35.0 26.0 36.5 2.0 39.0 76-77 27.536577617026033 34.0 26.0 36.0 2.0 39.0 78-79 26.981676861058354 34.0 24.5 35.5 2.0 38.0 80-81 26.37490806427587 34.0 21.0 35.0 2.0 37.0 82-83 25.780776356459242 33.0 19.0 35.0 2.0 37.0 84-85 25.212053820802147 33.0 10.0 35.0 2.0 36.0 86-87 24.624643894536923 33.0 2.0 35.0 2.0 36.0 88-89 23.999946467806204 32.0 2.0 35.0 2.0 35.0 90-91 23.376019439168807 31.5 2.0 35.0 2.0 35.0 92-93 22.65444968904778 31.0 2.0 34.5 2.0 35.0 94-95 21.871835781849327 31.0 2.0 34.0 2.0 35.0 96-97 21.053173760846086 29.5 2.0 34.0 2.0 35.0 98-99 20.039865191971103 29.0 2.0 34.0 2.0 35.0 100-101 17.450082393028712 22.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1776422578161423 1101 2 0.11366513971469061 1101 3 -0.0032407152522146987 1101 4 -0.03728434632796507 1101 5 0.10856768304694953 1101 6 0.03637895654627954 1101 7 -0.0654743082583309 1101 8 0.15546902755111347 1101 9 0.20393990366186898 1101 10-11 0.11085267871959559 1101 12-13 0.232717917858686 1101 14-15 0.29322435813028136 1101 16-17 0.15861525255770914 1101 18-19 0.2988073917190235 1101 20-21 0.20797224762887367 1101 22-23 0.21665626445128083 1101 24-25 0.17863891170944868 1101 26-27 0.37788858555048677 1101 28-29 0.19667932986089198 1101 30-31 0.2233220028530596 1101 32-33 0.2777915590672251 1101 34-35 0.3709387280044254 1101 36-37 0.39251701399345507 1101 38-39 0.22104430257914487 1101 40-41 0.5992277879664982 1101 42-43 0.4449066814338778 1101 44-45 0.9493985656700659 1101 46-47 0.8611630770998815 1101 48-49 0.561383924503815 1101 50-51 0.47841013367871454 1101 52-53 0.8641341979372932 1101 54-55 1.0879744516945173 1101 56-57 0.8665931174899448 1101 58-59 0.9866282986182213 1101 60-61 1.1755015404470903 1101 62-63 1.2546863317829597 1101 64-65 1.2695930385481198 1101 66-67 1.3368585507633242 1101 68-69 1.2398220648151899 1101 70-71 1.1637648532996927 1101 72-73 1.076949737623064 1101 74-75 1.1120069056496007 1101 76-77 1.3029573740544507 1101 78-79 1.244649016637009 1101 80-81 1.314341005485911 1101 82-83 1.2633402881995615 1101 84-85 1.4948057819542875 1101 86-87 1.4431476426244707 1101 88-89 1.4580054641715208 1101 90-91 1.572192289492996 1101 92-93 1.617926334792351 1101 94-95 1.7300973019639976 1101 96-97 1.2549529306917009 1101 98-99 1.4389077968953217 1101 100-101 1.5054155964926785 1102 1 0.20770414994700204 1102 2 0.3379504329654992 1102 3 0.07368236167086195 1102 4 0.05112164791357543 1102 5 0.21175524114166677 1102 6 0.22232740244167815 1102 7 0.15013928533934973 1102 8 0.0018722559172061892 1102 9 0.36385057948766075 1102 10-11 0.222672262024588 1102 12-13 0.2837732580386927 1102 14-15 0.5593831919283687 1102 16-17 0.39585141751270925 1102 18-19 0.504184979059481 1102 20-21 0.27683574487433305 1102 22-23 0.5377471982743174 1102 24-25 0.519215664753041 1102 26-27 0.4947128164330721 1102 28-29 0.3222206026170156 1102 30-31 0.7570792859472064 1102 32-33 0.884266428141558 1102 34-35 0.8345572639041379 1102 36-37 0.6950629796693875 1102 38-39 0.7999737727553011 1102 40-41 0.688841018826416 1102 42-43 0.8196034553348923 1102 44-45 0.48766739969486395 1102 46-47 0.6587837440839408 1102 48-49 1.009217699923795 1102 50-51 0.838914792591865 1102 52-53 0.540764817538733 1102 54-55 0.8310144208618091 1102 56-57 0.8181392872058701 1102 58-59 0.9238916689054619 1102 60-61 1.0979946824362479 1102 62-63 1.0402006259179437 1102 64-65 0.9420124994291186 1102 66-67 0.9968259043646306 1102 68-69 1.0950670261609474 1102 70-71 1.1510123538097936 1102 72-73 1.3190261846066704 1102 74-75 1.3192945340247633 1102 76-77 1.5210931286623541 1102 78-79 1.5120547614963336 1102 80-81 1.7731532660223088 1102 82-83 2.0794265744450016 1102 84-85 1.3414867314203818 1102 86-87 1.5723049574581793 1102 88-89 1.4655662794246425 1102 90-91 1.687525894879652 1102 92-93 1.8614423972739331 1102 94-95 1.5961960573200464 1102 96-97 1.6016055412700396 1102 98-99 1.6169440253294276 1102 100-101 1.320504353889124 1103 1 0.08653374655554202 1103 2 0.2212014563764768 1103 3 0.13832114494082504 1103 4 0.0701694750773072 1103 5 -0.014320250896851405 1103 6 0.05795521591137032 1103 7 -0.020803126851255627 1103 8 0.09610619091422024 1103 9 -0.09656781714418372 1103 10-11 0.16972804338612946 1103 12-13 0.24949445320789465 1103 14-15 0.26626153349270254 1103 16-17 0.12146801352395187 1103 18-19 0.00621359086257911 1103 20-21 0.04928887192298248 1103 22-23 0.0441061860807892 1103 24-25 0.01961993030087683 1103 26-27 -0.06822513791041729 1103 28-29 -0.33942734400536523 1103 30-31 -0.18044165573657267 1103 32-33 -0.19235828231690277 1103 34-35 0.12457226907581997 1103 36-37 0.14418379315365115 1103 38-39 0.3699444908183196 1103 40-41 0.42347164394477943 1103 42-43 0.31040004056573167 1103 44-45 0.7353364992388904 1103 46-47 0.654420589792899 1103 48-49 0.5588003230608933 1103 50-51 0.545581896302366 1103 52-53 0.7300812809800199 1103 54-55 0.9887945295400407 1103 56-57 1.1208941201822888 1103 58-59 0.888691429988171 1103 60-61 1.059061360757422 1103 62-63 1.0568702062101814 1103 64-65 0.9628194280307234 1103 66-67 1.2322395663549273 1103 68-69 1.341129989609513 1103 70-71 1.1410805326481324 1103 72-73 0.9768754918921907 1103 74-75 1.2855656760203047 1103 76-77 1.1030169956262093 1103 78-79 1.175527366131913 1103 80-81 1.004771930707708 1103 82-83 1.1486943803392649 1103 84-85 1.4691253835771931 1103 86-87 1.695933732424308 1103 88-89 1.8791699972108695 1103 90-91 1.678894279128592 1103 92-93 1.8635147134605816 1103 94-95 1.7537940644001573 1103 96-97 1.4725829169674896 1103 98-99 1.2120083436138849 1103 100-101 0.4542179153593402 1104 1 0.10712943730332469 1104 2 0.18313443011420816 1104 3 0.19383740043055298 1104 4 0.05009696645585393 1104 5 -0.04236576037308026 1104 6 -0.09222752939472656 1104 7 -0.032276289818810255 1104 8 0.0699912971717751 1104 9 0.10565312782589587 1104 10-11 0.2585695265706107 1104 12-13 0.24219569410747965 1104 14-15 0.30167580531161065 1104 16-17 0.18361983950326533 1104 18-19 0.16532727612799647 1104 20-21 0.07244520758484185 1104 22-23 0.23345021885705108 1104 24-25 0.12857813468087187 1104 26-27 0.06236406832650232 1104 28-29 0.17307376130899144 1104 30-31 0.21241653980090547 1104 32-33 0.2596673904510993 1104 34-35 0.4647474982443285 1104 36-37 0.4007677823068292 1104 38-39 0.4226348259496362 1104 40-41 0.6193186094884453 1104 42-43 0.8322226302435709 1104 44-45 0.7170067029114833 1104 46-47 0.7032460090754782 1104 48-49 0.9537512471036962 1104 50-51 0.8354063723833605 1104 52-53 1.0570037910752212 1104 54-55 1.351385533754975 1104 56-57 1.6197409088756594 1104 58-59 1.7301377706607894 1104 60-61 1.6507435018250085 1104 62-63 1.6140333800834945 1104 64-65 1.5967282617030207 1104 66-67 1.7124433863213255 1104 68-69 1.5075792777870731 1104 70-71 1.71222593306668 1104 72-73 1.8959775345131114 1104 74-75 1.676585365353283 1104 76-77 1.5683064406110319 1104 78-79 1.7383534338655728 1104 80-81 1.9093022457959883 1104 82-83 1.9362050650446605 1104 84-85 1.8449617381030876 1104 86-87 1.4060392531010564 1104 88-89 1.240114083042215 1104 90-91 1.5679722995842766 1104 92-93 1.7967426734924103 1104 94-95 1.6575432906801133 1104 96-97 1.5537350966473902 1104 98-99 1.5287545929138524 1104 100-101 1.5133338112534673 1105 1 0.10304138698885978 1105 2 -0.006992818679123047 1105 3 0.11896538053878913 1105 4 0.08515620296309123 1105 5 0.10452615128408382 1105 6 0.1904037416305755 1105 7 0.19762717661384244 1105 8 0.15495546274146932 1105 9 0.24241632805991742 1105 10-11 0.3581490205006972 1105 12-13 0.380827403921721 1105 14-15 0.3596398246183199 1105 16-17 0.11254700053881095 1105 18-19 0.4374568651290218 1105 20-21 0.21668610271428435 1105 22-23 0.4041427740713246 1105 24-25 0.2747350748593149 1105 26-27 0.37356049950977166 1105 28-29 0.3381571312080638 1105 30-31 0.16505658660505418 1105 32-33 0.2746264368164617 1105 34-35 0.4366247836033992 1105 36-37 0.5874688207739993 1105 38-39 0.5058401791747329 1105 40-41 0.7338059577406071 1105 42-43 0.6692600735666545 1105 44-45 0.9377577633180891 1105 46-47 1.0784251812270043 1105 48-49 1.0774458499996982 1105 50-51 1.0774313833530869 1105 52-53 0.9259740996901122 1105 54-55 0.8687213535589855 1105 56-57 0.5973809556798138 1105 58-59 0.5889281758092437 1105 60-61 0.8867534477077008 1105 62-63 0.8912126984980056 1105 64-65 0.8021802143138146 1105 66-67 0.6725903806170734 1105 68-69 0.6881957762513125 1105 70-71 0.753940135202118 1105 72-73 1.0219648096337792 1105 74-75 0.9108364988913848 1105 76-77 0.8129495589377811 1105 78-79 1.0412494408861974 1105 80-81 1.5055359740393683 1105 82-83 1.6112562570879518 1105 84-85 1.3816298673995604 1105 86-87 1.5035454692777428 1105 88-89 1.4082287532191948 1105 90-91 1.7336004990869824 1105 92-93 1.6146123531764829 1105 94-95 1.34014530910925 1105 96-97 1.1124889360506387 1105 98-99 1.315071691056314 1105 100-101 0.9481773098639685 1106 1 -0.0793784992046156 1106 2 -0.756603480553931 1106 3 -0.24973033674183753 1106 4 -0.007581143547838565 1106 5 -0.09058466033616241 1106 6 0.014130905452077513 1106 7 -0.09267680661466926 1106 8 -0.11430179991202039 1106 9 -0.006565402997281922 1106 10-11 -0.43988931433008815 1106 12-13 -0.23381021213098307 1106 14-15 -0.3839323526802687 1106 16-17 -0.1171138260559843 1106 18-19 -0.24690971169038534 1106 20-21 -0.22395106410212406 1106 22-23 -0.017910984539035724 1106 24-25 0.02674166365834907 1106 26-27 0.035832137724803204 1106 28-29 0.011944193202673148 1106 30-31 -0.16878962483549032 1106 32-33 0.07498208221927882 1106 34-35 0.05089552334583658 1106 36-37 0.0967118576113748 1106 38-39 0.14596446788392825 1106 40-41 0.1280856822090115 1106 42-43 -0.2785424122075213 1106 44-45 -0.08174283566363982 1106 46-47 -0.07270914042618415 1106 48-49 0.21286438848427736 1106 50-51 0.0027421051260709817 1106 52-53 -0.014517065329357592 1106 54-55 -0.03994890699807385 1106 56-57 -0.04122716052264508 1106 58-59 -0.21188419316349894 1106 60-61 -0.030665689758603065 1106 62-63 0.021932974029599706 1106 64-65 0.41026646768308694 1106 66-67 0.4566986465649592 1106 68-69 0.6646483069491254 1106 70-71 0.7953746964479969 1106 72-73 0.6119243783669326 1106 74-75 0.516886214375063 1106 76-77 0.5103514756793217 1106 78-79 0.6446149694875736 1106 80-81 0.2171187832636896 1106 82-83 -0.0013410774433424422 1106 84-85 0.024779013850597664 1106 86-87 -0.07924129322600137 1106 88-89 0.329653581011943 1106 90-91 0.0028652490121068297 1106 92-93 -0.2281511987851843 1106 94-95 -0.2540707735502288 1106 96-97 -0.1406632053090533 1106 98-99 0.4184971178307997 1106 100-101 0.08240911579388666 1107 1 0.20083890552640682 1107 2 0.14693861073134684 1107 3 0.32854617490043125 1107 4 0.4479342662477279 1107 5 0.17446227932166636 1107 6 0.29804635230527055 1107 7 0.2412509927800528 1107 8 0.16892653681826175 1107 9 0.22058239516218947 1107 10-11 0.21527404803171635 1107 12-13 0.2965384383538563 1107 14-15 0.5532819947967198 1107 16-17 0.7307762054429929 1107 18-19 0.6459474311667606 1107 20-21 0.6890523019654466 1107 22-23 0.6803743282890764 1107 24-25 0.5709174403244006 1107 26-27 0.5281967229882198 1107 28-29 0.46337554395309155 1107 30-31 0.5650015245684941 1107 32-33 0.446735679897003 1107 34-35 0.47153044398076105 1107 36-37 0.5034771902035544 1107 38-39 0.6042360385095762 1107 40-41 0.42360479776703386 1107 42-43 0.17793417972627168 1107 44-45 0.06636799751527889 1107 46-47 0.18133245675743837 1107 48-49 -0.32378019801438995 1107 50-51 -0.2523571784265215 1107 52-53 -0.08406187417377708 1107 54-55 0.2250131089367038 1107 56-57 0.01172278080278133 1107 58-59 0.05819254700078602 1107 60-61 0.09499439917969354 1107 62-63 0.03137494796574103 1107 64-65 0.11343489458691991 1107 66-67 0.5198703160109481 1107 68-69 0.6556541126436457 1107 70-71 0.5730907115181019 1107 72-73 0.7294202093841626 1107 74-75 0.7949419243373903 1107 76-77 0.7755991441371073 1107 78-79 0.7445972127091203 1107 80-81 0.9683617577556909 1107 82-83 0.8578901312528444 1107 84-85 1.049121241013836 1107 86-87 1.085811822702599 1107 88-89 1.5091244290139194 1107 90-91 1.6828186181678113 1107 92-93 1.5643994466351145 1107 94-95 1.7055479961366338 1107 96-97 1.8540020408153381 1107 98-99 2.3396917630040406 1107 100-101 2.2939155082341323 1108 1 0.04093896111284678 1108 2 0.17212667817622673 1108 3 0.22796807595657498 1108 4 0.10591091994422186 1108 5 -0.23574339049489623 1108 6 0.025480111801286398 1108 7 0.10835493941707597 1108 8 0.03696804135781662 1108 9 0.04121237478049267 1108 10-11 0.19265036820959125 1108 12-13 0.1894437561229836 1108 14-15 0.07840891716099208 1108 16-17 0.1868147453725868 1108 18-19 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-1.4632702464042282 2316 94-95 -1.1574437894232474 2316 96-97 -0.9056102952561389 2316 98-99 -0.4635796017459235 2316 100-101 0.4038376872224596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1709.0 3 219.0 4 172.0 5 180.0 6 200.0 7 258.0 8 365.0 9 421.0 10 610.0 11 1151.0 12 2121.0 13 3586.0 14 3983.0 15 6016.0 16 8856.0 17 12681.0 18 9617.0 19 5140.0 20 4530.0 21 4324.0 22 4669.0 23 5196.0 24 5548.0 25 5857.0 26 6571.0 27 7660.0 28 8922.0 29 10322.0 30 12230.0 31 14467.0 32 17730.0 33 22642.0 34 30560.0 35 48334.0 36 50448.0 37 61696.0 38 45854.0 39 4803.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.86562730610071 22.183871540265102 16.55348285428713 16.397018299347053 2 14.409428417369464 25.05257850595345 41.17673147264167 19.361261604035416 3 17.00044815416503 33.927604429256995 32.182604149160646 16.889343267417324 4 9.689978572528956 20.494979249431637 40.21735578471694 29.59768639332247 5 8.664069484352716 42.9331042708788 33.295908387467236 15.10691785730125 6 22.0428915763856 42.4130070070397 18.811206596837383 16.73289481973731 7 21.44633807154924 40.7747123003836 22.38270348972868 15.396246138338482 8 25.529909185092542 33.18010845521322 20.739026143119816 20.55095621657442 9 28.51998870523223 16.59794127187486 22.258860208579822 32.623209814313086 10-11 22.185243833704607 31.149170845211604 26.051015395171518 20.614569925912267 12-13 25.65776500499982 30.979626174549114 27.812996262678947 15.549612557772122 14-15 22.446053405262127 30.132743156342457 30.635109153187795 16.78609428520762 16-17 15.587689723158768 34.04540522883442 30.93108718223923 19.435817865767586 18-19 14.834493787903355 29.63294330083905 39.63843742818849 15.894125483069105 20-21 16.982646390458548 28.08832655956175 40.074752574577595 14.85427447540211 22-23 16.642111488673706 28.752305328765253 39.9442171680939 14.661366014467141 24-25 15.823296452047526 29.640072090568147 39.89886323206047 14.637768225323851 26-27 14.75135047492625 29.882968560267166 40.83000987995908 14.5356710848475 28-29 14.498771458639107 29.99169305366614 40.775206098605324 14.734329389089432 30-31 14.718213142624442 30.527658273561777 39.94571844301078 14.808410140803 32-33 14.033409212093776 31.339133722022382 40.051034397463916 14.576422668419928 34-35 14.57086620144746 31.19939982755543 39.51792525146222 14.711808719534888 36-37 14.613207591191829 30.956691755988075 39.10831027386713 15.321790378952969 38-39 13.947504400294402 32.021079422252136 39.12255388538122 14.90886229207224 40-41 13.871331578315067 31.948991719844035 39.2910254959754 14.888651205865507 42-43 13.904789457291274 32.00946762680565 37.9638303059714 16.121912609931677 44-45 14.195162635529607 32.00907516170235 37.50048117020594 16.2952810325621 46-47 14.904121542874943 32.54279444550088 36.75871667411357 15.7943673375106 48-49 14.594679733986698 32.95251429238129 35.605296931513244 16.847509042118773 50-51 14.641486448167903 33.01344622734311 35.113522192751425 17.231545131737555 52-53 14.920452264356172 33.793315936156404 34.234703166504055 17.051528632983366 54-55 15.241818718506048 33.14024237290706 33.926140082533266 17.691798826053628 56-57 15.774236855580218 33.39231414338052 33.08800115706586 17.745447843973402 58-59 15.583849113464632 32.8396538995777 33.875296085202564 17.701200901755108 60-61 16.186481893789807 33.31148466375014 33.85532992851753 16.646703513942523 62-63 16.2447892245713 33.37602261832281 34.38306984946779 15.996118307638115 64-65 16.45460525088168 33.26316452389394 34.19708999645463 16.085140228769756 66-67 16.34052638085752 33.49467344372884 33.623551146370076 16.541249029043552 68-69 16.025379348954385 33.1683364629865 34.27273469482966 16.53354949322945 70-71 16.501941940073944 33.05520241663654 34.12041194788836 16.32244369540116 72-73 15.96337551758325 32.88913512567796 34.20505044614218 16.942438910596604 74-75 15.045730089243953 33.78054456190446 33.81892027765822 17.35480507119337 76-77 14.639879518493935 32.880438903580966 34.9343872743159 17.545294303609197 78-79 14.42901504599656 32.634344254454426 34.92169035903681 18.014950340512204 80-81 14.771866998722713 33.02518998932677 34.037455455303665 18.165487556646855 82-83 15.079068856038463 32.194286140847304 33.967299388583946 18.759345614530286 84-85 14.705031709120425 31.879619630499533 33.85377185760168 19.56157680277837 86-87 14.759352301512882 31.83829463656763 33.9427082604416 19.459644801477893 88-89 15.151667137115115 31.153407407580207 33.85572551315817 19.839199942146504 90-91 15.217971971283104 30.543719782815355 34.16237526754419 20.075932978357354 92-93 15.824570612078267 30.743300382002158 34.00600705683375 19.42612194908582 94-95 15.5801969758469 29.67164471566336 34.35168726468571 20.39647104380403 96-97 15.601433815509983 29.520828934798253 34.304724289128686 20.573012960563076 98-99 15.886489910330903 29.72168401062451 33.90733065581975 20.484495423224843 100-101 16.47621113622527 28.207362103431464 35.00859557415403 20.307831186189233 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2960.0 1 2099.0 2 1626.0 3 1912.0 4 1576.5 5 1669.0 6 3057.5 7 6373.0 8 9555.5 9 9862.0 10 7256.5 11 4716.0 12 4167.0 13 4002.5 14 3645.5 15 3305.0 16 3046.0 17 2911.5 18 2774.5 19 2663.5 20 2664.0 21 2680.0 22 2714.0 23 2907.5 24 3087.5 25 3437.5 26 4226.0 27 5134.5 28 5438.5 29 5793.5 30 7095.0 31 8481.5 32 10489.0 33 13186.5 34 15223.5 35 17010.5 36 20046.0 37 22059.5 38 21098.5 39 19245.5 40 17311.0 41 15171.5 42 12966.0 43 12404.0 44 12412.5 45 11581.0 46 10265.5 47 9834.5 48 11376.5 49 10221.0 50 7001.0 51 4785.0 52 3242.0 53 2602.0 54 2273.0 55 2086.5 56 1983.5 57 1865.5 58 1764.0 59 1605.0 60 1444.0 61 1421.5 62 1245.5 63 757.5 64 404.5 65 342.0 66 307.5 67 225.5 68 156.5 69 134.0 70 111.0 71 83.5 72 83.0 73 105.5 74 110.5 75 71.0 76 43.0 77 37.0 78 28.0 79 23.0 80 17.0 81 8.5 82 5.5 83 4.5 84 2.0 85 1.5 86 1.0 87 0.0 88 0.5 89 1.0 90 0.5 91 1.0 92 1.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3342270882210554 2 0.2888410978289204 3 0.28488437046140097 4 0.2853498677987562 5 0.2874446058168547 6 0.2511358135031468 7 0.2504375674971139 8 0.2522995568465348 9 0.26323874427438276 10-11 0.27219956801847095 12-13 0.2635878672773992 14-15 0.2504375674971139 16-17 0.24741183480430493 18-19 0.23472703236137488 20-21 0.24066212341265408 22-23 0.2377527650541839 24-25 0.2375200163855063 26-27 0.23333054034930922 28-29 0.25392879752727815 30-31 0.2656826052954977 32-33 0.2591656425725245 34-35 0.25707090455442594 36-37 0.24345510743678547 38-39 0.22890831564443453 40-41 0.22634808028898076 42-43 0.2396147544036048 44-45 0.23402878635534205 46-47 0.24636446579525567 48-49 0.2555580382080215 50-51 0.27080307600640524 52-53 0.2853498677987562 54-55 0.2545106691989722 56-57 0.2275118236323688 58-59 0.2161071388671657 60-61 0.21913287155997468 62-63 0.2255334599486091 64-65 0.21412877518340595 66-67 0.22041298923770156 68-69 0.22180948124976724 70-71 0.22180948124976724 72-73 0.22646445462331954 74-75 0.23077030499385545 76-77 0.2407784977469929 78-79 0.239498380069266 80-81 0.23402878635534205 82-83 0.22599895728596434 84-85 0.21110304249059697 86-87 0.21645626187018213 88-89 0.22786094663538523 90-91 0.22821006963840168 92-93 0.22937381298178974 94-95 0.22483521394257625 96-97 0.23309779168063158 98-99 0.23658902171079582 100-101 0.21855099988828064 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 429648.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.428443377107616 #Duplication Level Percentage of deduplicated Percentage of total 1 76.50800096864499 23.280193753701088 2 10.196015120914886 6.204977375577833 3 3.79266960799502 3.462150972448602 4 1.9316382016514497 2.351069745760365 5 1.2147449493194458 1.8481398953997106 6 0.865443818744001 1.580046494083177 7 0.6179989282213969 1.3163321776148578 8 0.45961265726074274 1.1188238173488385 9 0.3815932857236223 1.0450160718953119 >10 3.2920496981406533 20.533032204072434 >50 0.4672336967226264 9.846395824615065 >100 0.2584697045699635 14.108761802384674 >500 0.005352922875709303 1.1373755267680885 >1k 0.006882329411626247 4.42315290268711 >5k 7.647032679584719E-4 2.0048337844995605 >10k+ 0.0015294065359169437 5.739697651143298 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14063 3.2731445276133018 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10604 2.468066882657431 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8616 2.005362529326332 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4974 1.1576918780024579 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2667 0.6207406993631996 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2313 0.5383476706513238 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2085 0.4852809741928276 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1889 0.439662235132015 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1441 0.3353908315644435 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1358 0.3160726920642014 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1145 0.26649722563586936 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1137 0.2646352362864484 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 807 0.18782817562283544 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 799 0.18596618627341452 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 782 0.18200945890589507 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 779 0.1813112128998622 No Hit GTACATGGGAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG 604 0.14058019588127957 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 600 0.1396492012065691 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 517 0.12033106170632704 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 484 0.11265035563996573 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 480 0.11171936096525528 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 461 0.1072971362603806 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 454 0.1056678955796373 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 453 0.10543514691095966 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 443 0.10310766022418351 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 441 0.10264216288682827 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 437 0.10171116821211784 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 436 0.10147841954344021 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.4912300301642274E-4 0.0 20-21 0.0 0.0 0.0 6.982460060328455E-4 0.0 22-23 0.0 0.0 0.0 0.0017456150150821138 0.0 24-25 0.0 0.0 0.0 0.004538599039213496 0.0 26-27 0.0 0.0 0.0 0.007680706066361301 0.0 28-29 0.0 0.0 0.0 0.012568428108591219 0.0 30-31 0.0 0.0 0.0 0.029209957919040703 0.0 32-33 0.0 0.0 0.0 0.055161434476594794 0.0 34-35 0.0 0.0 0.0 0.08076378803113246 0.0 36-37 0.0 0.0 0.0 0.1104392432875284 0.0 38-39 0.0 0.0 0.0 0.1532649983242096 0.0 40-41 0.0 0.0 0.0 0.21401240084906714 0.0 42-43 0.0 0.0 0.0 0.2795311510818158 0.0 44-45 0.0 0.0 0.0 0.34947212601943917 0.0 46-47 0.0 0.0 0.0 0.4364037537705284 0.0 48-49 0.0 0.0 0.0 0.5430026440248762 0.0 50-51 0.0 0.0 0.0 0.6636828287342196 0.0 52-53 1.1637433433880758E-4 0.0 0.0 0.8072887573083083 0.0 54-55 2.3274866867761517E-4 0.0 0.0 0.9683508360332179 0.0 56-57 2.3274866867761517E-4 0.0 0.0 1.1697948087736938 0.0 58-59 2.3274866867761517E-4 0.0 0.0 1.3920697873608163 0.0 60-61 2.3274866867761517E-4 0.0 0.0 1.6761395374818457 0.0 62-63 2.3274866867761517E-4 0.0 0.0 1.9375162924068072 0.0 64-65 2.3274866867761517E-4 0.0 0.0 2.2161164488139127 0.0 66-67 2.3274866867761517E-4 0.0 0.0 2.549761665363274 0.0 68-69 2.3274866867761517E-4 0.0 0.0 2.93565895803076 0.0 70-71 2.3274866867761517E-4 0.0 0.0 3.389984359289465 0.0 72-73 2.3274866867761517E-4 0.0 0.0 3.811608572598965 0.0 74-75 2.3274866867761517E-4 0.0 0.0 4.290838081406174 0.0 76-77 2.3274866867761517E-4 0.0 0.0 4.748421964026365 0.0 78-79 2.3274866867761517E-4 0.0 0.0 5.280718169292072 0.0 80-81 2.3274866867761517E-4 0.0 0.0 5.796721967750344 0.0 82-83 2.3274866867761517E-4 0.0 0.0 6.362533981305627 0.0 84-85 2.3274866867761517E-4 0.0 0.0 6.95848694745466 0.0 86-87 2.3274866867761517E-4 0.0 0.0 7.582020630841992 0.0 88-89 2.3274866867761517E-4 0.0 0.0 8.26339235839571 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAGAGC 20 0.0020709846 71.26909 3 CGTCACT 90 0.0 58.118595 1 ATGGGCG 50 4.2278225E-7 57.015266 5 ACTGGGC 65 2.584944E-6 43.84766 8 GTCACTA 120 1.8189894E-12 43.558414 2 GAGGCGC 105 1.024091E-9 40.715687 9 TAGGACA 275 0.0 39.737915 4 CATTCCG 60 8.280364E-5 39.584694 9 GGACAAT 185 0.0 38.510345 6 CCTTTAG 75 6.8608297E-6 38.04126 1 TAGGCAT 75 6.899436E-6 38.010178 5 CATGGAT 115 2.4883775E-9 37.183872 4 GGAGGCG 115 2.4938345E-9 37.175194 8 GCGTCAA 45 5.686379E-7 36.952183 26-27 GACAATG 195 0.0 36.53972 7 GGTATCA 5450 0.0 35.685493 1 GTCGGAT 40 1.0770338E-5 35.62623 76-77 GCACTGG 95 8.688585E-7 34.99712 6 GTATCAA 8410 0.0 33.359608 1 TTCGCGA 50 1.2941946E-6 33.26473 48-49 >>END_MODULE