##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937187_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 429648 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.779575373328864 31.0 31.0 34.0 30.0 34.0 2 31.937199754217406 31.0 31.0 34.0 30.0 34.0 3 31.899994879529288 31.0 31.0 34.0 30.0 34.0 4 35.737817934681416 37.0 35.0 37.0 33.0 37.0 5 35.69296261125386 37.0 35.0 37.0 33.0 37.0 6 35.78883877034223 37.0 35.0 37.0 35.0 37.0 7 35.69822971362604 37.0 35.0 37.0 33.0 37.0 8 35.709052526719546 37.0 35.0 37.0 33.0 37.0 9 37.26209594831118 39.0 37.0 39.0 34.0 39.0 10-11 37.17537495810524 39.0 37.0 39.0 33.0 39.0 12-13 37.049745372956465 39.0 37.0 39.0 33.0 39.0 14-15 37.873679151305254 40.0 37.0 41.0 32.5 41.0 16-17 37.9008909619037 39.0 37.0 41.0 32.5 41.0 18-19 38.1323920977172 40.0 37.0 41.0 33.5 41.0 20-21 38.16427051912263 40.0 37.0 41.0 33.5 41.0 22-23 38.02727465273898 40.0 37.0 41.0 33.0 41.0 24-25 37.95862776039921 40.0 37.0 41.0 33.0 41.0 26-27 37.681939634305294 39.5 36.0 41.0 33.0 41.0 28-29 37.42956327039809 39.0 36.0 41.0 33.0 41.0 30-31 37.01738632555022 38.5 35.0 40.0 32.0 41.0 32-33 36.49961480095334 38.0 35.0 40.0 31.5 41.0 34-35 36.149897125088444 38.0 35.0 40.0 31.0 41.0 36-37 35.776686962350574 38.0 35.0 40.0 30.5 41.0 38-39 35.498973578371135 38.0 35.0 40.0 30.0 41.0 40-41 35.09384193572413 37.5 35.0 40.0 29.0 41.0 42-43 34.56720966931069 37.0 34.0 40.0 26.0 41.0 44-45 33.79203091833315 37.0 33.0 40.0 21.5 41.0 46-47 32.85819205489145 37.0 33.0 40.0 10.5 41.0 48-49 32.11785461587159 36.0 33.0 40.0 2.0 41.0 50-51 31.701412551670202 36.0 32.0 40.0 2.0 41.0 52-53 31.42493855435147 35.5 31.0 40.0 2.0 41.0 54-55 31.168351767027893 35.0 31.0 40.0 2.0 41.0 56-57 30.790671666108068 35.0 31.0 39.0 2.0 41.0 58-59 30.5158408743902 35.0 30.5 39.0 2.0 40.5 60-61 30.12501629240681 35.0 29.0 39.0 2.0 40.0 62-63 29.711755902506237 35.0 28.0 38.5 2.0 40.0 64-65 29.26197142777343 35.0 28.0 38.0 2.0 40.0 66-67 28.775342838788966 35.0 26.5 38.0 2.0 40.0 68-69 28.298206904256507 34.0 26.0 37.0 2.0 39.5 70-71 27.822806111049047 34.0 25.5 36.5 2.0 39.0 72-73 27.30340185454139 33.5 24.5 36.0 2.0 39.0 74-75 26.775574423714296 33.0 23.0 36.0 2.0 38.5 76-77 25.521647953673707 31.5 20.5 34.5 2.0 36.5 78-79 26.06334720515399 33.0 20.5 35.0 2.0 37.0 80-81 25.760265380032024 33.0 19.0 35.0 2.0 37.0 82-83 25.23993711130972 33.0 15.0 35.0 2.0 36.0 84-85 24.65205354150374 32.0 6.0 35.0 2.0 36.0 86-87 24.22441393885227 32.0 2.0 35.0 2.0 35.5 88-89 23.646317450564183 31.5 2.0 35.0 2.0 35.0 90-91 22.92529931478792 31.0 2.0 34.0 2.0 35.0 92-93 22.240799445127173 30.5 2.0 34.0 2.0 35.0 94-95 21.565153334822924 30.0 2.0 34.0 2.0 35.0 96-97 20.72746643764198 29.0 2.0 34.0 2.0 35.0 98-99 19.67642581834432 28.0 2.0 34.0 2.0 35.0 100-101 17.281928462369194 21.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.04195248188542422 1101 2 0.1889723366887317 1101 3 -0.05649412988671543 1101 4 -0.035417401201442544 1101 5 0.013465779885819984 1101 6 0.12328076307905889 1101 7 0.17634916550638735 1101 8 -0.028699149367163557 1101 9 0.19636013107704997 1101 10-11 0.16104077817282558 1101 12-13 0.18521610479781714 1101 14-15 0.44638423429039165 1101 16-17 0.2214915340094521 1101 18-19 0.2677580646544939 1101 20-21 -0.0011289234935247805 1101 22-23 0.2641494996646614 1101 24-25 0.019205180580890158 1101 26-27 0.08915109226405349 1101 28-29 0.02832919618224139 1101 30-31 0.10358273767433701 1101 32-33 0.12154193009532577 1101 34-35 -0.01175878841108613 1101 36-37 0.13695675183312517 1101 38-39 0.2746204369042502 1101 40-41 0.40113455315562163 1101 42-43 0.3835620995163751 1101 44-45 0.48322868853427536 1101 46-47 0.6011219236018732 1101 48-49 0.2354386982268224 1101 50-51 -0.09839394194958118 1101 52-53 0.018830428249327014 1101 54-55 0.15768388059028027 1101 56-57 0.2789015964727497 1101 58-59 0.20364213411000165 1101 60-61 -0.15332368731389678 1101 62-63 0.15914190448804888 1101 64-65 0.04949685887611466 1101 66-67 -0.25084244408720124 1101 68-69 -0.13380895248194946 1101 70-71 0.25703371921345664 1101 72-73 0.27899430571434536 1101 74-75 0.5331914918459404 1101 76-77 0.614581154666265 1101 78-79 0.4980709585592997 1101 80-81 0.47254176822580973 1101 82-83 0.3080364592240912 1101 84-85 -0.018638469057950857 1101 86-87 -0.06751045511489906 1101 88-89 0.272753792454985 1101 90-91 0.22513982730466964 1101 92-93 0.09795761042542495 1101 94-95 0.13630500864187312 1101 96-97 0.6645346073192364 1101 98-99 1.193825639331834 1101 100-101 1.3198101618473927 1102 1 0.23069028943108805 1102 2 0.29736291457533426 1102 3 0.20711420401339709 1102 4 0.20776426240462342 1102 5 0.16074864732783567 1102 6 0.1479809376466079 1102 7 0.2040986043964068 1102 8 0.1399965816627855 1102 9 0.30655306222489287 1102 10-11 0.1319707471133995 1102 12-13 -0.016109021593621264 1102 14-15 0.16669641547790093 1102 16-17 0.4625704521440781 1102 18-19 0.46538213230508774 1102 20-21 0.45704287817960676 1102 22-23 0.3294649681631583 1102 24-25 0.07252196400377642 1102 26-27 0.21138238293035982 1102 28-29 0.25225741944733926 1102 30-31 0.49280035766118857 1102 32-33 0.7006130947102562 1102 34-35 0.4293813417210828 1102 36-37 0.6841466677233043 1102 38-39 0.618631863143797 1102 40-41 0.6278569907533864 1102 42-43 0.7565925468173802 1102 44-45 0.9883580272179913 1102 46-47 1.143159829698142 1102 48-49 1.4895478369513455 1102 50-51 1.4690072686877045 1102 52-53 1.1959031344997548 1102 54-55 1.1052285993551543 1102 56-57 1.3902564220185525 1102 58-59 1.2706636036513643 1102 60-61 1.4472056831093703 1102 62-63 1.4181820096819955 1102 64-65 1.4846234770266058 1102 66-67 1.4951334338515387 1102 68-69 1.5654644384360061 1102 70-71 1.4910052442990391 1102 72-73 1.5417014629838341 1102 74-75 1.438783321177823 1102 76-77 1.5461710796248944 1102 78-79 1.5904047226437292 1102 80-81 1.4075890374905846 1102 82-83 1.6065639706163282 1102 84-85 1.6872095438192396 1102 86-87 2.0712820815556228 1102 88-89 2.0299348666121872 1102 90-91 2.3724396980793436 1102 92-93 2.3527525502336317 1102 94-95 2.1782635658524185 1102 96-97 2.616024099259654 1102 98-99 2.7019192256747573 1102 100-101 3.1026549327558186 1103 1 0.2745330701953286 1103 2 0.26920272344149865 1103 3 0.2159352054265007 1103 4 0.11716507530615416 1103 5 0.21153314101255205 1103 6 0.21149069100820128 1103 7 0.3287216540882909 1103 8 0.11276146664741304 1103 9 0.39368503489508555 1103 10-11 0.3437002752517415 1103 12-13 0.3279610219650806 1103 14-15 0.4783305223788261 1103 16-17 0.5273876949801988 1103 18-19 0.43265483309031083 1103 20-21 0.4307656175703727 1103 22-23 0.4058857248317125 1103 24-25 0.3684188507948605 1103 26-27 0.40465045105093367 1103 28-29 0.4109553201801148 1103 30-31 0.6329566736730214 1103 32-33 0.40496750812459226 1103 34-35 0.5863055728881363 1103 36-37 0.47219497563525437 1103 38-39 0.4798048887982631 1103 40-41 0.7078687909369492 1103 42-43 0.7351737568460734 1103 44-45 1.4225028052725577 1103 46-47 1.489389598210586 1103 48-49 1.5374988297692624 1103 50-51 1.2960908751734337 1103 52-53 1.0793763218604475 1103 54-55 1.1367394598944678 1103 56-57 1.0974589044275405 1103 58-59 0.9906787545043301 1103 60-61 1.175389911654161 1103 62-63 1.168604485389647 1103 64-65 0.9490007624016634 1103 66-67 1.244732810335666 1103 68-69 1.4751129095109903 1103 70-71 1.504819471532695 1103 72-73 0.8509245447256006 1103 74-75 0.8823245416955743 1103 76-77 1.020032216230227 1103 78-79 1.0925280272778473 1103 80-81 1.179550490224294 1103 82-83 1.0007228797549104 1103 84-85 1.2142006426931218 1103 86-87 1.4692505561269549 1103 88-89 1.1701203188797145 1103 90-91 1.450921844838664 1103 92-93 1.70911809369764 1103 94-95 1.7991243965270698 1103 96-97 1.9802345795708334 1103 98-99 2.3501972729629017 1103 100-101 2.783150394783995 1104 1 0.2867136343477732 1104 2 0.24739855566951618 1104 3 0.24692085107745854 1104 4 0.2009479031423922 1104 5 0.09666150485256253 1104 6 0.038273194618895445 1104 7 0.12497537531327652 1104 8 0.1961758563665157 1104 9 0.18107413502182368 1104 10-11 0.0519230771499295 1104 12-13 0.11863659009665639 1104 14-15 0.016736511708856483 1104 16-17 -0.020745060642163082 1104 18-19 0.4660058514532679 1104 20-21 0.38530403829722104 1104 22-23 0.15301927628041767 1104 24-25 0.08858273206935507 1104 26-27 0.21818760435066054 1104 28-29 0.1888608231718294 1104 30-31 0.14331715352683005 1104 32-33 0.402675823036013 1104 34-35 0.457359600653632 1104 36-37 0.48207280155561705 1104 38-39 0.3948614808930557 1104 40-41 0.5021218931859011 1104 42-43 0.6047503477618719 1104 44-45 0.6752955662004325 1104 46-47 0.6655914433486814 1104 48-49 0.9423902142123239 1104 50-51 0.7072457076077754 1104 52-53 0.9840613809579217 1104 54-55 0.9446431943832216 1104 56-57 1.030793711513347 1104 58-59 1.0980047780254196 1104 60-61 1.3109898762841041 1104 62-63 1.3506755497336762 1104 64-65 0.981438054234129 1104 66-67 1.2244281857004218 1104 68-69 1.1222228809201908 1104 70-71 1.0953668231995088 1104 72-73 1.2432964019484594 1104 74-75 1.3497251370915748 1104 76-77 1.4034841866372787 1104 78-79 1.1796968014105467 1104 80-81 1.1511402270120996 1104 82-83 1.2417550959838763 1104 84-85 1.1047560999985144 1104 86-87 1.0068496659804609 1104 88-89 1.0602520723504014 1104 90-91 0.9602838573950336 1104 92-93 1.1460230661384685 1104 94-95 1.456152722049513 1104 96-97 1.7781131128923775 1104 98-99 2.0599785680965716 1104 100-101 2.0752112902359947 1105 1 0.040355582570679616 1105 2 0.21693928990967848 1105 3 0.1868872105918662 1105 4 -0.049425067458749083 1105 5 0.22192843479259494 1105 6 0.08158897639452789 1105 7 -0.11080054980007503 1105 8 -0.08387387304696858 1105 9 0.22855841175138636 1105 10-11 0.10600747088987816 1105 12-13 0.02910170637572662 1105 14-15 -0.11096569686215219 1105 16-17 -0.05028286962271267 1105 18-19 0.26174228556178747 1105 20-21 0.15111140995145433 1105 22-23 0.17737937573924256 1105 24-25 -0.14392421542223133 1105 26-27 0.06952956719985792 1105 28-29 0.10688042241126539 1105 30-31 0.07099742265071285 1105 32-33 0.11944487378926993 1105 34-35 0.11633283452813714 1105 36-37 -0.18655744562882148 1105 38-39 -0.030511489708082706 1105 40-41 -0.06332713416527724 1105 42-43 -0.0321481457294297 1105 44-45 -0.29518856426614803 1105 46-47 0.026619672103983305 1105 48-49 0.5866128264691639 1105 50-51 0.8059732196744704 1105 52-53 0.9014008573364514 1105 54-55 1.1989067602746992 1105 56-57 0.9873828588001672 1105 58-59 0.5592597144010227 1105 60-61 0.6265449442963877 1105 62-63 0.8576998293884976 1105 64-65 1.051651598186151 1105 66-67 0.5443382339672063 1105 68-69 0.925747818758424 1105 70-71 0.6297459368458469 1105 72-73 0.6130272780637149 1105 74-75 0.8927557060545688 1105 76-77 0.6867407319056689 1105 78-79 0.8861287159929638 1105 80-81 0.7106144115959836 1105 82-83 0.8191208996989552 1105 84-85 1.070973007999097 1105 86-87 1.3300798839123047 1105 88-89 1.440265236018675 1105 90-91 1.3763325629676508 1105 92-93 1.7148215157508702 1105 94-95 1.8209911014556859 1105 96-97 1.9342305620659914 1105 98-99 2.2717088591599044 1105 100-101 2.739223283800424 1106 1 -0.06549197657876604 1106 2 0.038742224920159885 1106 3 -0.11903522127396116 1106 4 0.01024100022618768 1106 5 0.07105242401092227 1106 6 -0.07786744768453246 1106 7 -0.007176710327179592 1106 8 -0.07634167777892031 1106 9 0.022343976291665513 1106 10-11 -0.23988201698074363 1106 12-13 -0.4990728967988787 1106 14-15 -0.20163281276511924 1106 16-17 -0.2758668768104897 1106 18-19 -0.16266247634296604 1106 20-21 -0.05099625701470245 1106 22-23 -0.1360864823020833 1106 24-25 -0.12021202614400295 1106 26-27 -0.20719999254855992 1106 28-29 -0.08333371355758601 1106 30-31 -0.016128213767380828 1106 32-33 0.005758141234615266 1106 34-35 -0.6999632121924293 1106 36-37 -0.2936516355060306 1106 38-39 -0.14108351726124368 1106 40-41 -0.41011509463248785 1106 42-43 -0.35759738202772695 1106 44-45 -0.33132588082799685 1106 46-47 -0.13516529345456973 1106 48-49 0.18101609091959858 1106 50-51 -0.04529212923238646 1106 52-53 -0.02618225137873864 1106 54-55 -0.02787895401101892 1106 56-57 -0.152299680882372 1106 58-59 0.3097987979589689 1106 60-61 0.3939079272254098 1106 62-63 0.2078645493645368 1106 64-65 -0.004326988216963201 1106 66-67 0.1607054097683438 1106 68-69 0.6118177498755273 1106 70-71 0.8571793001282657 1106 72-73 0.7710049659942335 1106 74-75 0.28231096211926143 1106 76-77 0.173394670210552 1106 78-79 0.4539446788561001 1106 80-81 0.33024229419556406 1106 82-83 0.1140557604357042 1106 84-85 0.4686819028778011 1106 86-87 0.4269539480033515 1106 88-89 0.7301948556652782 1106 90-91 0.8218102508981708 1106 92-93 0.6175992935286487 1106 94-95 0.483169148775243 1106 96-97 0.8142657522426902 1106 98-99 0.8225062535893741 1106 100-101 1.096736760889371 1107 1 0.22850623230270983 1107 2 0.24993980382201997 1107 3 0.30579184314454366 1107 4 0.24727855195245496 1107 5 0.1540846641443352 1107 6 0.30009716228396854 1107 7 0.27588177895698607 1107 8 0.16071903496272455 1107 9 0.3811389858648866 1107 10-11 0.26380058446450505 1107 12-13 0.1755997608504387 1107 14-15 0.44716513053671747 1107 16-17 0.3970301029931065 1107 18-19 0.4088239976240473 1107 20-21 0.44467649327867775 1107 22-23 0.2528217074329504 1107 24-25 0.3560170517387675 1107 26-27 0.5599854808625864 1107 28-29 0.31363836969854475 1107 30-31 0.47442595212725536 1107 32-33 0.7201056182280041 1107 34-35 0.8115130709278162 1107 36-37 0.82611783518756 1107 38-39 0.8327290946836641 1107 40-41 1.2247978199496856 1107 42-43 0.8432317823228388 1107 44-45 0.7301473141568735 1107 46-47 0.9744171906415318 1107 48-49 0.5441306298609874 1107 50-51 0.9746649456857739 1107 52-53 0.7271992568665837 1107 54-55 0.8179729081346174 1107 56-57 0.8397174891503596 1107 58-59 0.9905936840067753 1107 60-61 1.0550854332472746 1107 62-63 1.1877453473387192 1107 64-65 1.3970263871150124 1107 66-67 1.6827238150552297 1107 68-69 1.5470788517222296 1107 70-71 1.6417200943966854 1107 72-73 1.569322704243877 1107 74-75 1.7887728854099763 1107 76-77 1.7164008155999646 1107 78-79 1.6006504104353674 1107 80-81 1.6564150136075853 1107 82-83 1.6403087713676996 1107 84-85 1.4218503297816412 1107 86-87 1.6993699507209072 1107 88-89 1.6379599720879838 1107 90-91 1.9034451097703844 1107 92-93 1.8733818270349154 1107 94-95 2.013583422136989 1107 96-97 2.2976400033698567 1107 98-99 2.7768443117698425 1107 100-101 2.554247878176753 1108 1 -0.016841182927972653 1108 2 0.1285834947614326 1108 3 8.854136466744933E-4 1108 4 -0.0012669362817518959 1108 5 0.10081432877282737 1108 6 0.0476087427916525 1108 7 0.1001248769744052 1108 8 -0.028563900001145726 1108 9 -0.024163960216270652 1108 10-11 -0.12869154079026401 1108 12-13 0.13529218256620368 1108 14-15 0.07134337771107369 1108 16-17 -0.033724019667637606 1108 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-0.6436114361221676 2316 74-75 -0.37073136592307065 2316 76-77 -0.44201538269949836 2316 78-79 -0.23871405130263312 2316 80-81 -0.5655910391377681 2316 82-83 -0.5420950332150909 2316 84-85 -0.8903128061169063 2316 86-87 -0.9838264752770733 2316 88-89 -0.6310485305781057 2316 90-91 -0.7821580030700837 2316 92-93 -0.45184515091579414 2316 94-95 -0.3739075708014745 2316 96-97 0.08562818610512224 2316 98-99 0.032889851472972964 2316 100-101 0.2961415227941373 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 6.0 5 24.0 6 76.0 7 150.0 8 357.0 9 599.0 10 1091.0 11 1859.0 12 2825.0 13 3945.0 14 4942.0 15 7430.0 16 10217.0 17 13484.0 18 10379.0 19 5635.0 20 4689.0 21 4859.0 22 5006.0 23 5528.0 24 6247.0 25 6899.0 26 7868.0 27 8933.0 28 10590.0 29 12411.0 30 14169.0 31 17023.0 32 20119.0 33 24973.0 34 32737.0 35 46468.0 36 48364.0 37 56385.0 38 31679.0 39 1680.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.60765521272112 23.423190572324714 17.632183747459013 15.336970467495151 2 15.319982869697984 26.757717945853347 39.36524783078241 18.557051353666257 3 17.491527948460135 33.58609838751722 32.93742784791271 15.984945816109933 4 10.417597661341377 22.262875656351248 39.109922541243066 28.209604141064315 5 8.832640901209137 43.459134867157765 33.44202772056 14.266196511073096 6 22.27521133579116 43.35944773395896 17.63047890366067 16.73486202658921 7 21.104020035005398 41.31498342829479 22.09948609093956 15.48151044576025 8 25.738511525714074 34.462397125088444 20.22073883737385 19.57835251182363 9 27.667765240382824 17.446607455405356 22.284986779875616 32.6006405243362 10-11 22.481775779242543 31.809644173835327 25.755618552861876 19.952961494060254 12-13 25.93812446100687 31.481156043531445 27.61146174356205 14.969257751899642 14-15 22.327463411909285 30.923569992179644 30.654628905522664 16.09433769038841 16-17 15.42483614493725 34.320071314192084 31.358693628272444 18.89639891259822 18-19 14.851808922652962 29.70815644434514 39.912556325177825 15.52747830782408 20-21 16.866733775944233 28.152613479654832 40.77295922820451 14.207693516196418 22-23 16.54173751028469 28.798061648785563 40.60366326966427 14.056537571265478 24-25 15.890100733623806 29.652762261199868 40.653744460581684 13.803392544594645 26-27 14.893020278723343 29.70306362922231 41.545372552442466 13.858543539611881 28-29 14.516046440176153 29.97860940162187 41.517661791130934 13.987682367071047 30-31 14.884893877997518 30.244247021391978 40.73274106417594 14.13811803643456 32-33 14.151368272828307 31.27889723552447 40.60235425552342 13.9673802361238 34-35 14.660605891334303 30.768210255837335 40.596371913752655 13.97481193907571 36-37 14.879156891222584 30.650439429486465 39.808750418947604 14.661653260343352 38-39 14.003672773991733 31.657892041857522 40.06000260678509 14.278432577365658 40-41 14.073962871932371 31.52336331136186 40.120400886306925 14.282272930398838 42-43 14.178509538113465 31.66681602398407 38.62391866272053 15.530755775181934 44-45 14.672243324768184 31.630893196290916 37.95804937995755 15.738814098983353 46-47 15.194764085949428 32.38127490410755 36.95641548430343 15.467545525639592 48-49 15.070709045544259 32.698045842177784 35.739954567459876 16.49129054481808 50-51 15.012405504040515 33.010510929877476 34.934178676497964 17.042904889584033 52-53 15.34407235690612 33.7995289166946 34.0625349123003 16.793863814098984 54-55 15.655955572934122 33.264090604401744 33.47868487692251 17.60126894574163 56-57 16.332672327114288 33.43562637321715 32.765077458756934 17.46662384091163 58-59 16.190346516217925 32.69280899713254 33.577719435444834 17.539125051204707 60-61 16.64665028116039 33.542923509477525 33.36370703459576 16.44671917476632 62-63 16.877886083491603 33.274680668826576 33.99794715674226 15.84948609093956 64-65 16.836340446132645 33.45634100472945 33.69409376978364 16.013224779354264 66-67 16.96714519792947 33.5635217666555 33.026686962350574 16.442646073064463 68-69 16.543077123598852 33.46832756116635 33.55688842959818 16.431706885636615 70-71 17.007527091945036 33.49672289874502 33.43702286522921 16.058727144080738 72-73 16.499494351751963 33.17785667985193 33.54804472979531 16.774604238600805 74-75 15.548425688005066 34.34066957137005 32.76065523405206 17.350249506572823 76-77 15.054533013071165 33.35439708784866 34.33101050161993 17.260059397460246 78-79 14.645477227870257 33.246518079916584 34.152026775406846 17.955977916806315 80-81 15.052554649387407 33.78568037090828 33.19519699847317 17.966567981231147 82-83 15.10645354622103 33.28100361342729 33.109351270518275 18.50319156983341 84-85 14.62943459463863 33.065943593294506 32.88102456083185 19.423597251235027 86-87 14.383402226939262 33.34345790042081 32.84851785647786 19.42462201616207 88-89 14.638494767809929 32.614023572785165 32.85654768554724 19.89093397385767 90-91 14.675913694920226 32.217296629195175 33.01300814332816 20.093781532556438 92-93 15.067573307699739 32.71809600728044 32.67689915732658 19.537431527693244 94-95 14.76348080288981 31.838854131754367 32.918458831415485 20.479206233940342 96-97 14.854136409339738 31.770775146166162 32.70921777827431 20.665870666219792 98-99 14.886389423888188 32.02974531447291 32.140533809692826 20.943331451946072 100-101 14.814133749214403 31.52300924093946 33.0357766346221 20.62708037522404 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4941.0 1 3295.0 2 1905.0 3 2197.0 4 1887.5 5 1834.5 6 3032.5 7 5873.5 8 8257.0 9 8668.0 10 6971.5 11 4897.0 12 4423.5 13 4146.0 14 3700.0 15 3308.0 16 3006.5 17 2767.0 18 2663.0 19 2637.0 20 2637.0 21 2676.5 22 2754.0 23 2969.5 24 3186.0 25 3552.0 26 4435.5 27 5327.0 28 5535.5 29 6007.0 30 7310.0 31 8493.0 32 10336.5 33 13126.5 34 15109.5 35 16891.5 36 19821.0 37 21781.5 38 21085.0 39 19384.5 40 17418.5 41 15167.5 42 13171.5 43 12772.5 44 12806.0 45 11802.0 46 10198.5 47 9937.5 48 11558.0 49 10135.0 50 6824.5 51 4535.0 52 2888.0 53 2277.5 54 2057.0 55 1951.5 56 1922.0 57 1803.5 58 1717.5 59 1602.0 60 1368.5 61 1342.5 62 1152.0 63 699.5 64 412.0 65 312.5 66 289.5 67 239.0 68 161.0 69 115.0 70 105.0 71 80.5 72 59.5 73 90.0 74 86.5 75 57.5 76 46.5 77 37.5 78 36.0 79 22.5 80 11.5 81 8.5 82 5.5 83 1.0 84 0.5 85 0.5 86 0.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04398949838006927 2 0.0 3 0.0 4 0.0 5 6.982460060328455E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006400588388634417 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.1637433433880757E-4 22-23 0.0010473690090492683 24-25 0.0 26-27 0.0024438610211149595 28-29 0.004654973373552303 30-31 2.3274866867761514E-4 32-33 1.1637433433880757E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.006866085725989648 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 3.4912300301642274E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.1637433433880757E-4 84-85 1.1637433433880757E-4 86-87 0.0 88-89 0.0 90-91 0.007564331732022493 92-93 8.146203403716531E-4 94-95 0.0 96-97 0.0 98-99 1.1637433433880757E-4 100-101 0.008844449409749376 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 429648.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.33703525889384 #Duplication Level Percentage of deduplicated Percentage of total 1 76.85636980620446 23.31594400681408 2 9.8546114809204 5.979193919187645 3 3.750721252790787 3.413572886765897 4 1.8826416013053737 2.284550585546459 5 1.241538877238809 1.883230434704061 6 0.843748520431082 1.5358097168374347 7 0.6246634157305433 1.3265305247570987 8 0.48981543770491065 1.1887638563203513 9 0.43281006388105403 1.181715675152727 >10 3.2853099093545524 20.358280277661784 >50 0.4566778634882568 9.56317226638549 >100 0.26722971101252124 14.354687669881447 >500 0.005390801086707922 1.0345131506499632 >1k 0.005390801086707922 3.315675391604399 >5k 0.001540228881916549 3.73620919268162 >10k+ 0.001540228881916549 5.5281504450495556 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12653 2.944968904777865 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11009 2.5623300934718656 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8698 2.024447920157897 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7294 1.697668789334525 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3073 0.7152366588463114 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2613 0.6081722712546084 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2111 0.4913324395784456 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1910 0.44454995717424495 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1790 0.41662011693293116 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1507 0.35075224369716607 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1188 0.2765054183890068 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 781 0.18177671023721745 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 719 0.1673462927792053 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 663 0.15431236733325884 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 644 0.14989014262838418 No Hit GTACATGGGAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG 580 0.1349942278330168 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 524 0.12196030238707034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 517 0.12033106170632704 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 497 0.11567608833277472 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 491 0.11427959632070905 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 490 0.11404684765203142 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 462 0.1075298849290582 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 462 0.1075298849290582 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 449 0.1045041522362492 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 447 0.10403865489889397 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 445 0.10357315756153875 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 437 0.10171116821211784 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 435 0.1012456708747626 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.4912300301642274E-4 0.0 20-21 0.0 0.0 0.0 6.982460060328455E-4 0.0 22-23 0.0 0.0 0.0 0.0018619893494209215 0.0 24-25 0.0 0.0 0.0 0.004771347707891111 0.0 26-27 0.0 0.0 0.0 0.007913454735038915 0.0 28-29 0.0 0.0 0.0 0.013033925445946448 0.0 30-31 0.0 0.0 0.0 0.02990820392507355 0.0 32-33 0.0 0.0 0.0 0.055394183145272405 0.0 34-35 0.0 0.0 0.0 0.08076378803113246 0.0 36-37 0.0 0.0 0.0 0.11078836629054481 0.0 38-39 0.0 0.0 0.0 0.15373049566156483 0.0 40-41 0.0 0.0 0.0 0.21401240084906714 0.0 42-43 0.0 0.0 0.0 0.2795311510818158 0.0 44-45 0.0 0.0 0.0 0.3500539976911332 0.0 46-47 0.0 0.0 0.0 0.4375674971139165 0.0 48-49 0.0 0.0 0.0 0.5454465050459911 0.0 50-51 0.0 0.0 0.0 0.6678723047704167 0.0 52-53 0.0 0.0 0.0 0.8126419766878934 0.0 54-55 0.0 0.0 0.0 0.9748677987561911 0.0 56-57 0.0 0.0 0.0 1.178755632517782 0.0 58-59 0.0 0.0 0.0 1.4023107287826315 0.0 60-61 0.0 0.0 0.0 1.6883588425874203 0.0 62-63 0.0 0.0 0.0 1.9511320895244482 0.0 64-65 0.0 0.0 0.0 2.2305468662719248 0.0 66-67 0.0 0.0 0.0 2.5672178155140952 0.0 68-69 0.0 0.0 0.0 2.9531151081815814 0.0 70-71 0.0 0.0 0.0 3.409186124455368 0.0 72-73 0.0 0.0 0.0 3.8316249581052397 0.0 74-75 0.0 0.0 0.0 4.313065579264887 0.0 76-77 0.0 0.0 0.0 4.771696830894127 0.0 78-79 0.0 0.0 0.0 5.301200052135702 0.0 80-81 0.0 0.0 0.0 5.817436599262653 0.0 82-83 0.0 0.0 0.0 6.383481361486613 0.0 84-85 0.0 0.0 0.0 6.982809183331471 0.0 86-87 0.0 0.0 0.0 7.604480877369381 0.0 88-89 0.0 0.0 0.0 8.282361374892936 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCCAGA 35 2.7768302E-4 54.321854 1 GATAGTC 55 4.952258E-5 43.19044 7 CGCAGAA 45 9.6088834E-4 42.230656 9 AAGTTCG 35 4.3099044E-6 40.712936 14-15 AGATAGT 60 8.283694E-5 39.582024 6 CGACCGT 30 8.2906205E-5 39.582024 18-19 GGGCCGT 30 8.2906205E-5 39.582024 44-45 TCGACCG 30 8.2906205E-5 39.582024 18-19 GGTATCA 6125 0.0 36.783653 1 GCATCGA 55 0.002575311 34.552353 8 GTCAAGT 35 2.0504954E-4 33.935345 10-11 GCCGACC 35 2.0533017E-4 33.92745 52-53 GTGGTAC 100 1.2899563E-6 33.272133 1 GGTACTC 105 1.8984992E-6 31.66562 3 TCTAGAT 150 9.513315E-10 31.665619 3 TACACAG 75 3.07586E-4 31.665619 5 ACGCCAA 60 0.0039488617 31.665617 5 TAGATAG 95 3.486596E-5 29.99901 5 TCAGCGC 40 4.4935782E-4 29.686516 30-31 CCGCCAG 40 4.4935782E-4 29.686516 38-39 >>END_MODULE