FastQCFastQC Report
Thu 26 May 2016
SRR937186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420732
Sequences flagged as poor quality0
Sequence length101
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142193.3795860547807157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106822.538908378730403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85162.024091345559644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51721.2292861013661904No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26770.6362720211441012No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24110.573048876719622No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19910.47322285920728635No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19080.45349533669889625No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14950.3553330861450995No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14220.33798237357747923No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.2802258920167708No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10720.2547940256505329No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7750.18420277040966695No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA7540.17921146953405018No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7480.17778538356958823No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6390.15187815521519638No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5960.14165787246988581No Hit
GTACATGGGAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG5960.14165787246988581No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA5030.1195535400207258No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5030.1195535400207258No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4570.10862021429318426No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4470.10624340435241436No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4450.10576804236426039No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC4360.10362891341756748No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC4250.10101442248272058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4250.10101442248272058No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGT502.8010183E-547.5654141
GTTCGAC205.390394E-447.49454516-17
TCGACCG205.391978E-447.49171418-19
GTACCCT459.609404E-442.229968
GGTATCA53400.036.3421171
GTATCAA82900.033.5080871
TCTAGAT1151.0256008E-733.0731933
GTACAGA902.3867726E-531.7102781
GTGGTAC1051.8775845E-631.7102781
CGTCACT600.00392160231.7102781
TGTACCC600.003944576731.672477
GGACAAT1201.5011028E-731.6686926
CATGGGG8150.030.9173474
GGTACTC953.4642868E-530.0269783
GTATAGG2550.029.8449671
CCCAGTA650.005810905729.260552
GGATGCC650.005834668429.2361288
TGGGAGA3250.029.2326416
GTACATG49750.028.873881
ATGGGAG8900.028.3153325
Produced by FastQC (version 0.11.5)