##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937186_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 420732 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03346073034616 33.0 31.0 34.0 31.0 34.0 2 32.07277792038637 33.0 31.0 34.0 30.0 34.0 3 32.01806613235979 33.0 31.0 34.0 30.0 34.0 4 35.733868590932 37.0 35.0 37.0 35.0 37.0 5 35.79977990739948 37.0 35.0 37.0 35.0 37.0 6 35.93250335130202 37.0 35.0 37.0 35.0 37.0 7 35.891897930273906 37.0 35.0 37.0 35.0 37.0 8 35.91232185809494 37.0 36.0 37.0 35.0 37.0 9 37.5378530751167 39.0 37.0 39.0 35.0 39.0 10-11 37.45818715952198 39.0 37.0 39.0 35.0 39.0 12-13 37.337441649315956 39.0 37.0 39.0 35.0 39.0 14-15 38.31909148816824 40.0 38.0 41.0 34.0 41.0 16-17 38.37736611429604 40.0 38.0 41.0 34.5 41.0 18-19 38.57114861717198 40.0 38.0 41.0 35.0 41.0 20-21 38.658682962075616 40.0 38.0 41.0 35.0 41.0 22-23 38.52269378131447 40.0 38.0 41.0 35.0 41.0 24-25 38.366521918941274 40.0 38.0 41.0 35.0 41.0 26-27 38.15217644486276 40.0 37.5 41.0 34.0 41.0 28-29 38.0487281690007 40.0 37.0 41.0 34.0 41.0 30-31 37.60671876634057 39.0 36.5 41.0 34.0 41.0 32-33 37.07368110816387 39.0 35.0 41.0 33.0 41.0 34-35 36.754877213998455 38.5 35.0 41.0 33.0 41.0 36-37 36.52505276518069 38.0 35.0 41.0 33.0 41.0 38-39 36.29712263388571 38.0 35.0 41.0 33.0 41.0 40-41 35.950707338638374 38.0 35.0 41.0 31.5 41.0 42-43 35.44302786571974 38.0 35.0 40.5 30.5 41.0 44-45 34.80420077388932 38.0 35.0 40.0 26.0 41.0 46-47 33.95208351159408 38.0 35.0 40.0 18.0 41.0 48-49 33.265341119762695 38.0 34.0 40.0 2.0 41.0 50-51 32.22345697498645 37.0 32.5 39.5 2.0 40.5 52-53 32.27140317351663 37.0 33.0 39.5 2.0 40.5 54-55 32.50330495422264 37.0 33.0 40.0 2.0 41.0 56-57 32.27266407119021 37.0 33.0 40.0 2.0 41.0 58-59 32.0399399142447 37.0 33.0 40.0 2.0 41.0 60-61 31.753037563104304 36.0 33.0 40.0 2.0 41.0 62-63 31.415849044047043 36.0 33.0 40.0 2.0 41.0 64-65 31.04273147752013 35.0 32.0 39.5 2.0 41.0 66-67 30.654568704068147 35.0 31.5 39.0 2.0 41.0 68-69 30.184127900896534 35.0 31.0 39.0 2.0 40.5 70-71 29.73345383759733 35.0 30.5 38.0 2.0 40.0 72-73 29.32199713832083 35.0 29.5 37.5 2.0 40.0 74-75 28.85468540543624 35.0 29.0 37.0 2.0 39.0 76-77 28.41683066655258 35.0 28.5 36.5 2.0 39.0 78-79 27.90145864826065 35.0 27.5 36.0 2.0 39.0 80-81 27.357378331099135 34.5 26.0 35.5 2.0 37.5 82-83 26.802502543186637 34.0 24.5 35.0 2.0 37.0 84-85 26.24999049276024 34.0 21.0 35.0 2.0 36.5 86-87 25.763573961571737 34.0 16.5 35.0 2.0 36.0 88-89 25.24554942338591 33.5 4.5 35.0 2.0 36.0 90-91 24.726805900192996 33.0 2.0 35.0 2.0 35.0 92-93 24.20266701843454 33.0 2.0 35.0 2.0 35.0 94-95 23.618869018757785 32.5 2.0 35.0 2.0 35.0 96-97 23.010312978333 32.0 2.0 35.0 2.0 35.0 98-99 22.38770761434833 31.5 2.0 35.0 2.0 35.0 100-101 20.881000256695472 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10882557771304846 1101 2 0.03519322530327429 1101 3 0.08584232073921072 1101 4 0.012064447093635522 1101 5 0.017555609559209984 1101 6 0.276049413986172 1101 7 0.19667169934393058 1101 8 0.25412379574663646 1101 9 0.27579966725387806 1101 10-11 0.26125836703121763 1101 12-13 0.18523840889973542 1101 14-15 0.0973857192559251 1101 16-17 0.48979609097398935 1101 18-19 0.2810578769762486 1101 20-21 0.2954272782014087 1101 22-23 0.23271259704773684 1101 24-25 0.22736207033025835 1101 26-27 0.19374233292484888 1101 28-29 0.23348487777499827 1101 30-31 0.3791415197498722 1101 32-33 0.42451720460704223 1101 34-35 0.412940838051135 1101 36-37 0.6027797421665966 1101 38-39 0.5293847153477174 1101 40-41 0.765691434224884 1101 42-43 0.9960514576435173 1101 44-45 1.2273430437741268 1101 46-47 1.5986715568912473 1101 48-49 1.6004246873447059 1101 50-51 1.5365732732792559 1101 52-53 1.2833405988962099 1101 54-55 1.0046060033877424 1101 56-57 0.9154812222731863 1101 58-59 1.340302539962508 1101 60-61 1.1308287010276104 1101 62-63 1.38330207944648 1101 64-65 1.2378571944616645 1101 66-67 1.078844995084438 1101 68-69 1.1496503042663555 1101 70-71 1.2074310021072954 1101 72-73 0.9220865972171204 1101 74-75 1.3884597695208818 1101 76-77 1.5592868818737315 1101 78-79 1.707637431303688 1101 80-81 1.5195956206569647 1101 82-83 1.8690866703925693 1101 84-85 2.0339473318809738 1101 86-87 1.8991330028849163 1101 88-89 1.8727529481936678 1101 90-91 2.1527449731588426 1101 92-93 2.057928104624846 1101 94-95 1.828534333122068 1101 96-97 1.5879504121415664 1101 98-99 1.5626155466676863 1101 100-101 1.7526499704464236 1102 1 -0.12397586741716182 1102 2 -0.11042622374083066 1102 3 -0.017638787193948957 1102 4 -0.16996927261131844 1102 5 -0.22335587621528674 1102 6 -0.4772021289914008 1102 7 -0.1065045764350856 1102 8 -0.057828635383877725 1102 9 -0.1513454479049372 1102 10-11 0.018380525694475125 1102 12-13 0.04859833664322366 1102 14-15 0.3215841235913075 1102 16-17 0.16597214095141055 1102 18-19 0.012596714850701574 1102 20-21 0.07719568103145491 1102 22-23 0.0537497790438195 1102 24-25 -9.158161668025855E-4 1102 26-27 -0.06456152075572419 1102 28-29 -0.1410766907934189 1102 30-31 0.31872077610994864 1102 32-33 0.3433490576854723 1102 34-35 0.21838453818661918 1102 36-37 0.28734432142295674 1102 38-39 0.44259787205403 1102 40-41 0.6914497512503246 1102 42-43 0.9076426062209677 1102 44-45 0.47067813334209774 1102 46-47 0.888312908690132 1102 48-49 1.0535256959251669 1102 50-51 0.7680914126741065 1102 52-53 1.0316564953285479 1102 54-55 1.225799364820837 1102 56-57 1.2055045551047385 1102 58-59 1.1828943659447475 1102 60-61 1.155744025429236 1102 62-63 1.00277370371262 1102 64-65 1.1902412829758866 1102 66-67 1.1775221740698356 1102 68-69 1.2391956903870813 1102 70-71 1.4829014220982621 1102 72-73 1.4273797629554927 1102 74-75 1.520171721952014 1102 76-77 1.4009886732330585 1102 78-79 1.3017590630965508 1102 80-81 1.2775325467445775 1102 82-83 1.3230400799966588 1102 84-85 1.4560777699360727 1102 86-87 1.4053651269252612 1102 88-89 1.4284807820036924 1102 90-91 1.2629060449637457 1102 92-93 1.330935405543105 1102 94-95 1.314636244908911 1102 96-97 1.5349566593190467 1102 98-99 1.756409766146838 1102 100-101 1.8021682603756695 1103 1 0.012163464948585556 1103 2 0.019109335040013775 1103 3 0.14299256258834703 1103 4 0.021395023469956698 1103 5 0.06971378996758659 1103 6 0.15044663733661423 1103 7 0.04124578640961829 1103 8 0.2153208566335394 1103 9 0.27009867401088883 1103 10-11 0.11542334136936461 1103 12-13 0.1652166240162245 1103 14-15 0.10075683766968524 1103 16-17 0.15847939784546128 1103 18-19 0.22437102544576248 1103 20-21 0.026742125153369045 1103 22-23 0.12261770357212498 1103 24-25 0.09719739138036232 1103 26-27 0.2966685227856445 1103 28-29 0.4121707692936596 1103 30-31 0.3399471912042884 1103 32-33 0.21257982691624022 1103 34-35 0.4508409968223219 1103 36-37 0.34324054783805025 1103 38-39 0.4266689896157132 1103 40-41 0.7897988078540976 1103 42-43 0.7844205452630533 1103 44-45 1.0109176108457163 1103 46-47 1.2589769144956477 1103 48-49 1.6517114724128348 1103 50-51 1.4495500483780255 1103 52-53 1.4079445940222968 1103 54-55 1.4109771587250393 1103 56-57 1.4961794462513183 1103 58-59 1.3266352948271845 1103 60-61 1.3098985972724932 1103 62-63 1.0725850244673438 1103 64-65 1.288916899811884 1103 66-67 1.2067130303833338 1103 68-69 1.004725443652685 1103 70-71 1.130942060704939 1103 72-73 1.342274537991777 1103 74-75 1.2426136667996168 1103 76-77 1.292098978022608 1103 78-79 1.3864470166524292 1103 80-81 1.3108779749013273 1103 82-83 1.4586170030735843 1103 84-85 1.6098694970768541 1103 86-87 1.6941169411487742 1103 88-89 1.827174541074811 1103 90-91 2.024734782264183 1103 92-93 1.8166937509712646 1103 94-95 1.4145273914981722 1103 96-97 1.5101562239054012 1103 98-99 1.4948350201381295 1103 100-101 1.8099331370084677 1104 1 -0.0010298153595797999 1104 2 -0.041260890019728436 1104 3 0.03930101213715176 1104 4 -0.10384305478377343 1104 5 -0.16306432466664944 1104 6 0.04092350718716631 1104 7 -0.059100184985325654 1104 8 -0.009449981901255455 1104 9 0.06553327828380873 1104 10-11 -0.006403173593007239 1104 12-13 -0.014394247332347732 1104 14-15 0.24136745934818293 1104 16-17 0.227457890551527 1104 18-19 0.2069565374477591 1104 20-21 0.030850981307736447 1104 22-23 0.08692558244646875 1104 24-25 -0.1505377443331568 1104 26-27 -0.33392497865887805 1104 28-29 -0.24202368803059215 1104 30-31 0.06659412204567872 1104 32-33 0.17987661330565174 1104 34-35 0.048406713553781344 1104 36-37 0.014778158852074341 1104 38-39 0.07521020301229697 1104 40-41 -0.23659845290845993 1104 42-43 -0.14373153948210415 1104 44-45 -0.23755583495803734 1104 46-47 0.2597812088210034 1104 48-49 0.5034966134764218 1104 50-51 0.5919208441122343 1104 52-53 0.5106535143775446 1104 54-55 0.7400334058270843 1104 56-57 0.9308390032652696 1104 58-59 0.7728082091903445 1104 60-61 0.49455637092872706 1104 62-63 0.5803729475689572 1104 64-65 0.6981208089319715 1104 66-67 0.8541271617097941 1104 68-69 1.1810816871880156 1104 70-71 1.1743293703315025 1104 72-73 1.2103756187065464 1104 74-75 1.2126193735442783 1104 76-77 1.140326320462954 1104 78-79 1.3662130401214192 1104 80-81 1.435955550961534 1104 82-83 1.6339478159280247 1104 84-85 1.2154514066962854 1104 86-87 1.299854002888619 1104 88-89 1.1609513360243398 1104 90-91 1.1859538855919318 1104 92-93 1.3432354637080053 1104 94-95 1.4760692825706805 1104 96-97 1.4695884028118478 1104 98-99 1.5778253543393603 1104 100-101 1.7706065328782152 1105 1 0.21593704985424722 1105 2 0.3115621652286933 1105 3 0.29016237390909794 1105 4 0.330828985734108 1105 5 0.2961288843072083 1105 6 0.386295693940383 1105 7 0.27332125810773533 1105 8 0.3153208566335337 1105 9 0.23802320231277463 1105 10-11 0.586178058350491 1105 12-13 0.3708770013747227 1105 14-15 0.5290587244621392 1105 16-17 0.44149826576999374 1105 18-19 0.4205974405401065 1105 20-21 0.37957231383261814 1105 22-23 0.21695732621363817 1105 24-25 0.3434238064747035 1105 26-27 0.526857202030925 1105 28-29 0.45745378816158677 1105 30-31 0.52107926667599 1105 32-33 0.422013789180383 1105 34-35 0.5083881666336438 1105 36-37 0.6064480950078561 1105 38-39 0.7351595556534463 1105 40-41 0.6369686191748585 1105 42-43 0.7683828094139997 1105 44-45 0.9731817617891139 1105 46-47 1.104259933363572 1105 48-49 1.0337869441109504 1105 50-51 0.8816255200761383 1105 52-53 0.783416292135513 1105 54-55 0.9289016870269293 1105 56-57 0.8301417104022661 1105 58-59 0.985125860864926 1105 60-61 1.0787665218007874 1105 62-63 1.1084340810711097 1105 64-65 1.130426333774146 1105 66-67 1.1170903888739012 1105 68-69 1.0386877078036285 1105 70-71 1.096979796553999 1105 72-73 1.1790669908219655 1105 74-75 0.9029910252901843 1105 76-77 1.1307782233056258 1105 78-79 1.2090885260863935 1105 80-81 1.4278591069767934 1105 82-83 1.2822019087339598 1105 84-85 1.223077044246665 1105 86-87 1.4893999600166943 1105 88-89 1.3120802014521686 1105 90-91 1.3086970464151264 1105 92-93 1.434618279273149 1105 94-95 1.4805651273472265 1105 96-97 1.4318543371129486 1105 98-99 1.3844576616475628 1105 100-101 1.3344614388952643 1106 1 -0.09529645434468392 1106 2 -0.043114826082614854 1106 3 -0.08895566877094652 1106 4 -0.020532485891294527 1106 5 -0.07197378046902969 1106 6 -0.03513157911738318 1106 7 -0.14355808169668194 1106 8 -0.12368012493479341 1106 9 -0.06972028116415885 1106 10-11 -0.4923001471971844 1106 12-13 -0.5296883092734817 1106 14-15 -0.2023005659003161 1106 16-17 -0.36293515117841935 1106 18-19 -0.04987734769561314 1106 20-21 -0.008114456807582826 1106 22-23 0.022054210643048577 1106 24-25 -0.14089654834406673 1106 26-27 -0.34961969475897803 1106 28-29 0.1322911022998099 1106 30-31 0.14699630597507252 1106 32-33 0.21778213905496813 1106 34-35 0.16709322352824785 1106 36-37 0.1586111807678563 1106 38-39 -0.0639643037278006 1106 40-41 -0.020616722738118654 1106 42-43 -0.09499560335365942 1106 44-45 -0.1516391928769636 1106 46-47 0.4992084919123059 1106 48-49 0.49089786133201585 1106 50-51 0.37913706259258717 1106 52-53 0.47955000355442223 1106 54-55 0.441378511108077 1106 56-57 0.3464375623672211 1106 58-59 0.3589670649220693 1106 60-61 0.5527186067246248 1106 62-63 0.8063382509187882 1106 64-65 0.8720720966708626 1106 66-67 0.8440980596486121 1106 68-69 0.7228699159688219 1106 70-71 0.6434097961904506 1106 72-73 0.6331804892041966 1106 74-75 0.7704811187209479 1106 76-77 0.6540159446129294 1106 78-79 0.6094884242941241 1106 80-81 0.8558228980118052 1106 82-83 0.8232605483529696 1106 84-85 0.8967310232338335 1106 86-87 1.1667257316384685 1106 88-89 1.2091536736592445 1106 90-91 0.7273050371084047 1106 92-93 0.7048367691121022 1106 94-95 0.7230288638506615 1106 96-97 0.5206299215096486 1106 98-99 0.6305204820205574 1106 100-101 0.451857010931473 1107 1 0.13454221024985458 1107 2 -0.017962304681162777 1107 3 0.013230875058688696 1107 4 0.04409857443525311 1107 5 0.11069039666077174 1107 6 0.0978536662771603 1107 7 -0.09043553908944801 1107 8 0.17131881291250295 1107 9 0.158307133556157 1107 10-11 0.15449308334958545 1107 12-13 0.16099232563296084 1107 14-15 0.12973023280125062 1107 16-17 0.17659370024318832 1107 18-19 0.27802782629244405 1107 20-21 0.015633041543281934 1107 22-23 0.32096447923724725 1107 24-25 0.31059288789509765 1107 26-27 0.08662691846463133 1107 28-29 0.23055513482776746 1107 30-31 0.20774398694167218 1107 32-33 0.18666106482726263 1107 34-35 -0.12660690653886775 1107 36-37 0.12595650710961337 1107 38-39 0.28550443357761424 1107 40-41 0.33131919032188506 1107 42-43 0.17339175069351853 1107 44-45 -0.15494677366579168 1107 46-47 0.06271217963195852 1107 48-49 -0.24340184461939174 1107 50-51 -0.15376917354818076 1107 52-53 -0.1900646027223445 1107 54-55 -0.15397485032571723 1107 56-57 -0.14955793559605723 1107 58-59 -0.3119892079281925 1107 60-61 -0.17773871523435503 1107 62-63 -0.18649128661619585 1107 64-65 -0.0522914502483367 1107 66-67 0.17241318731191413 1107 68-69 -0.015000113808721238 1107 70-71 -0.03467780718309044 1107 72-73 -0.10466717946378878 1107 74-75 0.06897584336251938 1107 76-77 0.04115010754343018 1107 78-79 0.26140048550393047 1107 80-81 0.24155203789172575 1107 82-83 0.4194487245436065 1107 84-85 0.5413719969856174 1107 86-87 0.47294435620661446 1107 88-89 0.22249952921393046 1107 90-91 0.6469857950009441 1107 92-93 0.3892805178775802 1107 94-95 0.448808987060378 1107 96-97 0.5817521510613481 1107 98-99 0.5774743398709816 1107 100-101 0.6775365091481724 1108 1 0.05846989195167396 1108 2 -0.09829604622603583 1108 3 0.1417793587626548 1108 4 0.03565202916382759 1108 5 -0.07271485057426474 1108 6 -5.74910056485578E-4 1108 7 0.05917589693769543 1108 8 0.06386761708160549 1108 9 -0.13808114810217376 1108 10-11 -0.00935616069606482 1108 12-13 0.02453522027241206 1108 14-15 0.24337899538618046 1108 16-17 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Count 2 1492.0 3 207.0 4 154.0 5 162.0 6 191.0 7 225.0 8 319.0 9 425.0 10 566.0 11 1083.0 12 1961.0 13 3335.0 14 3868.0 15 5766.0 16 8502.0 17 12316.0 18 9467.0 19 4807.0 20 4218.0 21 3942.0 22 4192.0 23 4533.0 24 4727.0 25 5089.0 26 5591.0 27 6220.0 28 7088.0 29 8290.0 30 9634.0 31 11503.0 32 14256.0 33 17857.0 34 25276.0 35 43174.0 36 47411.0 37 65308.0 38 66857.0 39 10718.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.91042529796617 22.11878576250003 16.566609184298965 16.404179755234832 2 14.362513708100892 25.160205979116007 41.312163257521576 19.165117055261526 3 16.903621063672556 34.007962049154926 32.17335335764857 16.915063529523948 4 9.608929490969427 20.62751511452711 40.39607689813667 29.367478496366793 5 8.708753811420793 42.80210650283816 33.22939091171331 15.259748774027745 6 21.800991896179628 42.56196969047313 18.78218779329303 16.854850620054215 7 21.420048159024816 40.931122114805206 22.34392974784392 15.304899978326056 8 25.23854439093174 33.330951462230665 20.736569819787633 20.693934327049956 9 28.615320125944944 16.56004534780144 22.225662473265277 32.59897205298834 10-11 22.106671177008693 31.233205328556185 26.163358553133097 20.49676494130203 12-13 25.788840588340378 30.980408503543146 27.840647889001367 15.390103019115106 14-15 22.348934143146362 30.20554960104799 30.81005120876503 16.63546504704061 16-17 15.443594247774437 34.04007502902909 31.10608271057254 19.41024801262393 18-19 14.780359541570206 29.501533815389596 39.77714424533425 15.940962397705945 20-21 16.86661482898925 28.022710142045554 40.34658210695153 14.764092922013667 22-23 16.44264133957937 28.78507143486555 40.14498931870739 14.627297906847698 24-25 15.775724836553836 29.687014438984665 40.08684584327028 14.45041488119122 26-27 14.604497978195951 29.926152952214935 40.99963802801473 14.469711041574387 28-29 14.323942588472915 29.9858757734979 41.078633594222644 14.611548043806538 30-31 14.633431713748724 30.530682540930304 40.1562414393555 14.679644305965475 32-33 13.99094615211465 31.350705002006805 40.24877239493451 14.409576450944032 34-35 14.292673514502422 31.213434272211 39.92798814651065 14.565904066775929 36-37 14.46480070681981 30.988738036098297 39.28755992369775 15.258901333384136 38-39 13.752827986949583 32.19939987140101 39.31330523207354 14.734466909575861 40-41 13.781229647075072 32.03566755212432 39.51732833889176 14.665774461908851 42-43 13.782887490399442 32.204645181264475 38.05797843521336 15.954488893122726 44-45 14.156676364216636 32.02132587697727 37.712791358184944 16.10920640062115 46-47 14.776674864087555 32.59457302949285 36.880414912557534 15.748337193862058 48-49 14.586291006450505 32.96177812925786 35.80662296456509 16.645307899726543 50-51 14.51029294762769 33.153010228631594 35.33737333314277 16.99932349059795 52-53 14.761633025533746 33.955842215680505 34.44726524572938 16.835259513056368 54-55 15.175005984586562 33.21546774456713 34.14489487969492 17.464631391151386 56-57 15.608416786423984 33.52772317426148 33.38556220851942 17.478297830795114 58-59 15.453231448430888 32.94253377179718 34.00670554421825 17.597529235553672 60-61 16.038103129899955 33.51441963685112 34.042796372559174 16.404680860689748 62-63 16.09800473187059 33.546509620319185 34.522294207090184 15.833191440720046 64-65 16.216798644621527 33.36333644079804 34.49523938788898 15.924625526691456 66-67 16.160244016267754 33.55747526025545 33.867614983856065 16.414665739620737 68-69 15.853911155239043 33.286665666645234 34.50859661278456 16.35082656533116 70-71 16.330685729730117 33.14155967704872 34.39733580670549 16.130418786515676 72-73 15.722082783649016 32.9249794017212 34.55998266410756 16.79295515052222 74-75 14.915710737444721 33.86418462432398 34.14125923085899 17.078845407372302 76-77 14.377554896410969 33.1186019678284 35.20812470454591 17.295718431214716 78-79 14.193313313122605 32.72197071270204 35.263830972901275 17.820885001274085 80-81 14.688652016117508 33.101144038331476 34.28602387144096 17.924180074110062 82-83 14.860680008715313 32.26904123224046 34.38476677608433 18.485511982959896 84-85 14.545337655797407 32.084533301268046 34.14119599549023 19.228933047444322 86-87 14.482666660317022 32.06519021551488 34.36963504622938 19.082508077938716 88-89 14.947947919345467 31.294349039441478 34.165428465297026 19.59227457591603 90-91 15.057157451186074 30.701070280863892 34.4463984435742 19.79537382437583 92-93 15.643833594071829 30.917037534437718 34.24484654527444 19.194282326216005 94-95 15.317515119767608 29.970355731225297 34.74903566836516 19.963093480641938 96-97 15.327524278144134 29.9088163266278 34.4981207281836 20.265538667044467 98-99 15.642517967451408 30.003857822568737 34.138633949791156 20.2149902601887 100-101 16.113417391190474 28.812336528262083 35.089532213382036 19.98471386716541 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3244.0 1 2270.0 2 1673.0 3 2037.5 4 1692.0 5 1718.0 6 3146.0 7 6582.0 8 9918.5 9 10158.0 10 7315.0 11 4621.5 12 4091.5 13 3995.5 14 3652.5 15 3280.0 16 3003.0 17 2862.5 18 2699.5 19 2587.5 20 2630.5 21 2726.5 22 2819.5 23 2918.5 24 3051.0 25 3455.5 26 4218.0 27 5102.0 28 5387.5 29 5729.5 30 7052.0 31 8382.5 32 10286.0 33 12859.5 34 14752.5 35 16373.0 36 19176.0 37 21316.5 38 20723.5 39 19005.5 40 16848.0 41 14553.0 42 12505.0 43 11957.0 44 11883.5 45 11107.5 46 9668.5 47 9325.5 48 11355.5 49 10106.5 50 6715.0 51 4573.5 52 3039.5 53 2526.0 54 2194.5 55 2007.0 56 1898.0 57 1678.5 58 1574.0 59 1498.0 60 1385.0 61 1380.0 62 1195.0 63 681.5 64 344.0 65 312.0 66 297.0 67 229.5 68 181.5 69 121.5 70 90.0 71 83.5 72 87.0 73 106.0 74 99.5 75 70.0 76 44.0 77 33.0 78 25.0 79 17.5 80 15.0 81 8.0 82 4.0 83 1.5 84 1.0 85 1.0 86 0.0 87 0.0 88 0.5 89 1.0 90 1.0 91 0.5 92 0.5 93 2.0 94 2.0 95 1.0 96 1.0 97 2.0 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3501041042754057 2 0.3023302244659308 3 0.2951997946436211 4 0.30137950048962286 5 0.30161718148369987 6 0.22104332449160036 7 0.20749550782921197 8 0.21248680870482872 9 0.205831740870673 10-11 0.2272230303376021 12-13 0.21557666162782962 14-15 0.20963463677590488 16-17 0.21260564920186723 18-19 0.20535637888251906 20-21 0.1976317465750169 22-23 0.19941435403059432 24-25 0.1883621878060143 26-27 0.19252160520236158 28-29 0.21058536075221282 30-31 0.22175636747383132 32-33 0.21640854510709906 34-35 0.22342013443237027 36-37 0.1956114581253625 38-39 0.19537377713128548 40-41 0.18978827377047622 42-43 0.19834478955724782 44-45 0.2059505813677115 46-47 0.2102288392610973 48-49 0.21866651455083047 50-51 0.22104332449160036 52-53 0.24540562638449176 54-55 0.21474477814856013 56-57 0.18669842084747534 58-59 0.18562885637412888 60-61 0.18218248196001255 62-63 0.19335348868163107 64-65 0.18432161090670546 66-67 0.18063755549851213 68-69 0.1761216166110493 70-71 0.1883621878060143 72-73 0.18871870929712975 74-75 0.1922839242082846 76-77 0.1966810225987089 78-79 0.1956114581253625 80-81 0.19394769116682353 82-83 0.18574769687116738 84-85 0.17956799102516569 86-87 0.1808752364925891 88-89 0.18253900345112803 90-91 0.18907523078824526 92-93 0.18325204643335902 94-95 0.1787361075458962 96-97 0.17956799102516569 98-99 0.19157088122605362 100-101 0.1767158190962418 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 420732.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.611348715988985 #Duplication Level Percentage of deduplicated Percentage of total 1 76.43376556717928 23.397406514530765 2 10.181973455589342 6.2336788013197735 3 3.8267880675231467 3.5142943199140992 4 2.0202370630609003 2.473687249052906 5 1.1805831050138533 1.806962055789205 6 0.8885976426911026 1.6320703385173871 7 0.6351544637140518 1.3610054344087468 8 0.48602366212673087 1.1902271844466694 9 0.40759638140046556 1.1229367469782408 >10 3.2164841796270105 20.290122315250102 >50 0.4619375565887329 9.77669227201074 >100 0.24533154265889012 13.450616279814085 >500 0.0062109251306048125 1.2153727521771336 >1k 0.0062109251306048125 3.3640205919177406 >5k 0.0015527312826512031 3.2530352428237395 >10k+ 0.0015527312826512031 5.917871901048652 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14219 3.3795860547807157 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10682 2.538908378730403 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8516 2.024091345559644 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5172 1.2292861013661904 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2677 0.6362720211441012 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2411 0.573048876719622 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1991 0.47322285920728635 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1908 0.45349533669889625 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1495 0.3553330861450995 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1422 0.33798237357747923 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1179 0.2802258920167708 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1072 0.2547940256505329 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 775 0.18420277040966695 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 754 0.17921146953405018 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 748 0.17778538356958823 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 639 0.15187815521519638 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 596 0.14165787246988581 No Hit GTACATGGGAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG 596 0.14165787246988581 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 503 0.1195535400207258 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 503 0.1195535400207258 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 457 0.10862021429318426 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 447 0.10624340435241436 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 445 0.10576804236426039 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 436 0.10362891341756748 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 425 0.10101442248272058 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 425 0.10101442248272058 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1884049703849482E-4 0.0 12-13 0.0 0.0 0.0 4.7536198815397926E-4 0.0 14-15 0.0 0.0 0.0 5.94202485192474E-4 0.0 16-17 0.0 0.0 0.0 9.507239763079585E-4 0.0 18-19 0.0 0.0 0.0 9.507239763079585E-4 0.0 20-21 0.0 0.0 0.0 0.0010695644733464532 0.0 22-23 0.0 0.0 0.0 0.0019014479526159168 0.0 24-25 0.0 0.0 0.0 0.004278257893385813 0.0 26-27 0.0 0.0 0.0 0.008199994295656142 0.0 28-29 0.0 0.0 0.0 0.015686945609081314 0.0 30-31 0.0 0.0 0.0 0.03529562762043296 0.0 32-33 0.0 0.0 0.0 0.058113003051823965 0.0 34-35 0.0 0.0 0.0 0.07867240903948355 0.0 36-37 0.0 0.0 0.0 0.10707528783168382 0.0 38-39 0.0 0.0 0.0 0.1448665658899252 0.0 40-41 0.0 0.0 0.0 0.19406653166386204 0.0 42-43 0.0 0.0 0.0 0.24635635036079975 0.0 44-45 0.0 0.0 0.0 0.3117186237319719 0.0 46-47 0.0 0.0 0.0 0.40037363452268904 0.0 48-49 0.0 0.0 0.0 0.4999619710409477 0.0 50-51 0.0 0.0 0.0 0.6247444929313672 0.0 52-53 0.0 0.0 0.0 0.7718690282650238 0.0 54-55 0.0 0.0 0.0 0.925648631432836 0.0 56-57 0.0 0.0 0.0 1.1381354401376649 0.0 58-59 0.0 0.0 0.0 1.3633381820256125 0.0 60-61 0.0 0.0 0.0 1.6170626432027988 0.0 62-63 0.0 0.0 0.0 1.866390005989561 0.0 64-65 0.0 0.0 0.0 2.1361579342669446 0.0 66-67 0.0 0.0 0.0 2.4654649515606133 0.0 68-69 0.0 0.0 0.0 2.8462299040719508 0.0 70-71 0.0 0.0 0.0 3.3000817622619625 0.0 72-73 0.0 0.0 0.0 3.7136466919559243 0.0 74-75 0.0 0.0 0.0 4.16405217573182 0.0 76-77 0.0 0.0 0.0 4.602335928809788 0.0 78-79 0.0 0.0 0.0 5.128323968702167 0.0 80-81 0.0 0.0 0.0 5.658352585493853 0.0 82-83 0.0 0.0 0.0 6.241859425952863 0.0 84-85 0.0 0.0 0.0 6.855314071665573 0.0 86-87 0.0 0.0 0.0 7.478157116644325 0.0 88-89 0.0 0.0 0.0 8.162440698591977 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 50 2.8010183E-5 47.565414 1 GTTCGAC 20 5.390394E-4 47.494545 16-17 TCGACCG 20 5.391978E-4 47.491714 18-19 GTACCCT 45 9.609404E-4 42.22996 8 GGTATCA 5340 0.0 36.342117 1 GTATCAA 8290 0.0 33.508087 1 TCTAGAT 115 1.0256008E-7 33.073193 3 GTACAGA 90 2.3867726E-5 31.710278 1 GTGGTAC 105 1.8775845E-6 31.710278 1 CGTCACT 60 0.003921602 31.710278 1 TGTACCC 60 0.0039445767 31.67247 7 GGACAAT 120 1.5011028E-7 31.668692 6 CATGGGG 815 0.0 30.917347 4 GGTACTC 95 3.4642868E-5 30.026978 3 GTATAGG 255 0.0 29.844967 1 CCCAGTA 65 0.0058109057 29.26055 2 GGATGCC 65 0.0058346684 29.236128 8 TGGGAGA 325 0.0 29.232641 6 GTACATG 4975 0.0 28.87388 1 ATGGGAG 890 0.0 28.315332 5 >>END_MODULE