FastQCFastQC Report
Thu 26 May 2016
SRR937186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420732
Sequences flagged as poor quality0
Sequence length101
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127823.0380384662920816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109452.6014184801726516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87652.083273913084814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72661.7269901029634067No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31930.7589154140878278No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25790.6129792837245562No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21330.5069735603662189No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17540.4168924636110398No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17420.4140402916821159No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15300.36365192093779414No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.3047070344067007No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8010.1903824762556687No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7630.18135059848074309No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6630.1575824990730441No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5940.14118251048173186No Hit
GTACATGGGAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG5400.1283477368015744No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.1283477368015744No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5210.12383179791411159No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5160.12264339294372664No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.11931585902664879No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4790.11384919616287803No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4770.11337383417472406No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4710.11194774821026211No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT4480.10648108534649135No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4470.10624340435241436No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4460.10600572335833737No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA4240.10077674148864361No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA250.00501827457.0022774
TATCACG250.00501827457.0022772
AACAGCG352.7837208E-454.2943427
CAGCGCA405.3800445E-447.5075459
ACAGCGC405.3800445E-447.5075458
GTATGGT652.5720692E-643.87921
CATGGGT2050.039.3918234
GGTACTC756.9042962E-638.006048
GGTATCA58150.036.1321141
TGGTACT851.6279819E-533.534747
CATGAGA1001.2961937E-633.251334
GTATCAA85950.031.0821671
ATGGGTA1551.3515091E-930.6463895
TGGGTAT804.490925E-429.6851586
CTGGTCG1554.5658453E-827.585039
CCGCACC350.00838381427.14717190-91
CAGAGTC700.00838399227.1439424
CCGGCTA350.00839354227.14071732-33
GTACATG49400.026.1735611
TAGGACA2007.1486284E-1026.1260454
Produced by FastQC (version 0.11.5)