##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937186_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 420732 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.748956580436 31.0 31.0 34.0 30.0 34.0 2 31.923972980424594 31.0 31.0 34.0 30.0 34.0 3 31.889563902912066 31.0 31.0 34.0 30.0 34.0 4 35.71644895087609 37.0 35.0 37.0 33.0 37.0 5 35.59656741108354 37.0 35.0 37.0 33.0 37.0 6 35.738767196219925 37.0 35.0 37.0 33.0 37.0 7 35.67584352984798 37.0 35.0 37.0 33.0 37.0 8 35.69840658661571 37.0 35.0 37.0 33.0 37.0 9 37.24157658556991 39.0 37.0 39.0 34.0 39.0 10-11 37.171512982135894 39.0 37.0 39.0 33.0 39.0 12-13 37.071845022484624 39.0 37.0 39.0 33.0 39.0 14-15 37.86783391802858 40.0 37.0 41.0 32.5 41.0 16-17 37.922371010524515 39.0 37.0 41.0 32.5 41.0 18-19 38.169659070382096 40.0 37.0 41.0 33.5 41.0 20-21 38.21998920928287 40.0 37.0 41.0 34.0 41.0 22-23 38.06080830552466 40.0 37.0 41.0 33.0 41.0 24-25 37.87735304184136 40.0 36.5 41.0 33.0 41.0 26-27 37.56411920177215 39.0 36.0 41.0 33.0 41.0 28-29 37.34676706311856 39.0 35.5 41.0 32.5 41.0 30-31 36.99428258368748 38.5 35.0 40.0 32.0 41.0 32-33 36.51988439196448 38.0 35.0 40.0 31.5 41.0 34-35 36.190652244183944 38.0 35.0 40.0 31.0 41.0 36-37 35.83403568067083 38.0 35.0 40.0 30.5 41.0 38-39 35.521264130135094 38.0 35.0 40.0 30.0 41.0 40-41 35.15038433016743 38.0 35.0 40.0 29.5 41.0 42-43 34.60790479450101 37.0 34.5 40.0 26.0 41.0 44-45 33.85386897122159 37.0 33.0 40.0 21.5 41.0 46-47 32.96305724309062 37.0 33.0 40.0 11.5 41.0 48-49 32.22247059886104 36.5 33.0 40.0 2.0 41.0 50-51 31.84064083549623 36.0 33.0 40.0 2.0 41.0 52-53 31.583574579542322 36.0 32.0 40.0 2.0 41.0 54-55 31.342793036897596 35.5 31.5 40.0 2.0 41.0 56-57 31.04276594126427 35.0 31.0 40.0 2.0 41.0 58-59 30.7810553986861 35.0 31.0 39.5 2.0 41.0 60-61 30.430979816129984 35.0 30.5 39.0 2.0 41.0 62-63 30.020673492864816 35.0 29.0 39.0 2.0 40.0 64-65 29.645509492978903 35.0 28.5 38.5 2.0 40.0 66-67 29.224578829278496 35.0 28.0 38.0 2.0 40.0 68-69 28.734896561231377 35.0 26.5 37.5 2.0 40.0 70-71 28.34258744283772 34.5 26.0 37.0 2.0 39.5 72-73 27.8675843529848 34.0 26.0 36.5 2.0 39.0 74-75 27.38408060237871 34.0 25.0 36.0 2.0 39.0 76-77 26.122175161385393 32.0 23.0 35.0 2.0 37.0 78-79 26.627692925662892 33.0 24.0 35.0 2.0 37.5 80-81 26.344498398030098 33.0 21.5 35.0 2.0 37.0 82-83 25.90673160111425 33.0 19.5 35.0 2.0 37.0 84-85 25.41513600106481 33.0 15.0 35.0 2.0 36.0 86-87 24.96150399779432 33.0 4.5 35.0 2.0 36.0 88-89 24.41664289856726 32.5 2.0 35.0 2.0 35.0 90-91 23.784770352623525 32.0 2.0 35.0 2.0 35.0 92-93 23.189007491704935 31.0 2.0 34.5 2.0 35.0 94-95 22.591639095671354 31.0 2.0 34.0 2.0 35.0 96-97 22.023065752070202 31.0 2.0 34.0 2.0 35.0 98-99 21.29173202894004 30.0 2.0 34.0 2.0 35.0 100-101 19.929785469134746 27.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.01858483701078839 1101 2 0.15401102283081514 1101 3 0.12598059361776492 1101 4 0.08131394253638291 1101 5 -0.20064446503237576 1101 6 -0.061825547084680466 1101 7 -0.06585317517892264 1101 8 -0.023568236074574145 1101 9 -0.04015989897372663 1101 10-11 0.09848813480593321 1101 12-13 0.22024795588107082 1101 14-15 0.40613650736889895 1101 16-17 0.12883440195250984 1101 18-19 0.21343620681249575 1101 20-21 0.048899982175733214 1101 22-23 0.10008259487661064 1101 24-25 0.026765014603284953 1101 26-27 0.0485899174826514 1101 28-29 0.09771532289896356 1101 30-31 0.14037147105333503 1101 32-33 0.11184737220960272 1101 34-35 0.18237315926720044 1101 36-37 0.036504251845848046 1101 38-39 0.03883306617409943 1101 40-41 0.301006414584549 1101 42-43 0.5350326452972496 1101 44-45 0.6539940118246435 1101 46-47 0.6279146096961412 1101 48-49 0.5877041818055488 1101 50-51 0.7441601554399035 1101 52-53 0.7925717191187118 1101 54-55 0.7555138055915016 1101 56-57 0.7567473208935844 1101 58-59 0.7241928364399648 1101 60-61 0.9312552571661037 1101 62-63 1.2112375975524294 1101 64-65 1.0176372841071668 1101 66-67 0.6990296343625744 1101 68-69 0.8978288641653123 1101 70-71 0.9661922977151356 1101 72-73 0.6369133906812507 1101 74-75 1.0550541271096527 1101 76-77 0.9193697726123382 1101 78-79 1.089726663021981 1101 80-81 1.0946642546849397 1101 82-83 0.8628919710848635 1101 84-85 0.9935946424412982 1101 86-87 0.9939263107440581 1101 88-89 1.1142945973746414 1101 90-91 1.148104311107641 1101 92-93 1.5021573921716609 1101 94-95 1.50927189093591 1101 96-97 1.2877321086761384 1101 98-99 0.7342062221197416 1101 100-101 0.6400787359172604 1102 1 0.20207121364784797 1102 2 0.14398919534323795 1102 3 0.13043641177221232 1102 4 0.2004920247281632 1102 5 0.0258495888589394 1102 6 0.10172631201512417 1102 7 0.1644042386401381 1102 8 0.2917373487515533 1102 9 0.37602254871556795 1102 10-11 0.2893619869811559 1102 12-13 0.4023832863040724 1102 14-15 0.46620725853554035 1102 16-17 0.379122298986978 1102 18-19 0.21248595843074725 1102 20-21 0.32449082968439313 1102 22-23 0.25948723133603835 1102 24-25 0.4032929387339408 1102 26-27 0.38770439889165687 1102 28-29 0.451145625473103 1102 30-31 0.5804136206845314 1102 32-33 0.6187945346362156 1102 34-35 0.649577359176881 1102 36-37 0.4931145935589214 1102 38-39 0.39392865551626954 1102 40-41 0.7908810946989462 1102 42-43 0.8761078372286022 1102 44-45 0.6534464429551505 1102 46-47 0.7780877242528135 1102 48-49 0.6664750684531526 1102 50-51 0.9218490761082769 1102 52-53 1.019635922039086 1102 54-55 0.9253617658504218 1102 56-57 1.1424220913286298 1102 58-59 0.8574082511622301 1102 60-61 0.5855172622842701 1102 62-63 0.7913708204938708 1102 64-65 0.9652851841523251 1102 66-67 0.9799437544890246 1102 68-69 0.9105603108008715 1102 70-71 1.0798231873734245 1102 72-73 1.1512235668064932 1102 74-75 0.8532590044241211 1102 76-77 0.9044574588986549 1102 78-79 1.2864770017243714 1102 80-81 1.4678183266403835 1102 82-83 1.3253110588464168 1102 84-85 1.4920196763115392 1102 86-87 1.745926536545653 1102 88-89 1.9735769246364185 1102 90-91 1.5485032272599852 1102 92-93 1.2724795357814784 1102 94-95 1.688069121103002 1102 96-97 1.8218432030612064 1102 98-99 1.6730422148940889 1102 100-101 1.064339235691456 1103 1 0.02253565486700637 1103 2 -0.05815158840132284 1103 3 -0.0859641687793129 1103 4 -0.027809862064287927 1103 5 -0.10970919198285856 1103 6 -0.14025482006034906 1103 7 -0.13211245221617673 1103 8 -0.09102752788124491 1103 9 -0.046473822837405976 1103 10-11 -0.23875847746005974 1103 12-13 -0.07660800542306845 1103 14-15 0.062215966808402356 1103 16-17 -0.2592485282989401 1103 18-19 -0.14660330136607058 1103 20-21 -0.16172107162185512 1103 22-23 0.054153414209778816 1103 24-25 -0.11524842556213599 1103 26-27 -0.047437110542304595 1103 28-29 -0.13029486799569412 1103 30-31 8.780619036841131E-4 1103 32-33 0.1037001950135803 1103 34-35 0.08069129241345507 1103 36-37 0.21120240197692652 1103 38-39 0.07905202271510348 1103 40-41 -0.09397013897303452 1103 42-43 -0.09100029049272962 1103 44-45 0.4682686490509482 1103 46-47 0.47932139624120396 1103 48-49 0.5769612803544533 1103 50-51 0.5081154041198808 1103 52-53 0.30764390462253743 1103 54-55 0.5164357861697262 1103 56-57 0.5606187531573674 1103 58-59 0.3021107185062064 1103 60-61 0.55926544806076 1103 62-63 0.47805441991331676 1103 64-65 0.8189499156472486 1103 66-67 0.8915729272031037 1103 68-69 1.1240944182029011 1103 70-71 0.9674864674895325 1103 72-73 0.9118367743536595 1103 74-75 0.7551095124067082 1103 76-77 1.077371101859466 1103 78-79 1.27031227022945 1103 80-81 0.8021615777289206 1103 82-83 0.9024518425909704 1103 84-85 0.8856154673129915 1103 86-87 0.9834991054861462 1103 88-89 1.1095166924158093 1103 90-91 0.989286971817311 1103 92-93 1.155570972646501 1103 94-95 0.8427135332945852 1103 96-97 0.781132027444368 1103 98-99 0.8770153643861072 1103 100-101 1.030685389537613 1104 1 0.0749663714201354 1104 2 0.2552913619856696 1104 3 0.13095989584957124 1104 4 0.06041641036143375 1104 5 0.012122673052630262 1104 6 0.04799285265784192 1104 7 0.061685029682742254 1104 8 -0.016162171388039326 1104 9 0.0880626824948294 1104 10-11 0.22893956441317442 1104 12-13 0.13125125198678234 1104 14-15 -0.04476409525244662 1104 16-17 -0.1172914715524982 1104 18-19 0.17187050110923963 1104 20-21 0.0415549564838642 1104 22-23 0.09683273082710286 1104 24-25 -0.08459785667102437 1104 26-27 0.3589473100447762 1104 28-29 -0.03542737147324715 1104 30-31 0.00926340983677676 1104 32-33 0.11038243633753808 1104 34-35 0.09143245587602422 1104 36-37 0.5315415966125769 1104 38-39 0.07248034956889882 1104 40-41 0.2517997365485911 1104 42-43 -0.011813858278159728 1104 44-45 -0.03044733340081507 1104 46-47 0.26247044927826124 1104 48-49 0.12158267351350105 1104 50-51 -0.008587524153465864 1104 52-53 0.22952213695837997 1104 54-55 0.22931697887936053 1104 56-57 0.4270720113518962 1104 58-59 0.32730064610188236 1104 60-61 0.45286676061203224 1104 62-63 0.5171705878342792 1104 64-65 0.8967560289974621 1104 66-67 0.9167057873521891 1104 68-69 0.9228585513127214 1104 70-71 0.7519694720905967 1104 72-73 0.751058160379273 1104 74-75 0.644152562661727 1104 76-77 0.752056339224378 1104 78-79 0.9406157977109935 1104 80-81 0.9634359651455462 1104 82-83 0.9175533156444402 1104 84-85 0.8250918734905426 1104 86-87 1.150032614777441 1104 88-89 1.3877418948269096 1104 90-91 1.4504008570404103 1104 92-93 1.608272759570923 1104 94-95 1.4694781657445617 1104 96-97 1.5428843547261764 1104 98-99 1.4835246200125987 1104 100-101 1.4826702666642682 1105 1 0.12064886241417838 1105 2 0.1588295436741518 1105 3 -0.0029453008547832837 1105 4 0.17219013793571492 1105 5 0.2223662797152528 1105 6 0.2729527271094625 1105 7 0.22071773646307236 1105 8 0.17501020796780864 1105 9 0.0950356111248567 1105 10-11 0.1065245414078575 1105 12-13 0.18848633419957395 1105 14-15 0.14712162718576138 1105 16-17 0.40678920755011205 1105 18-19 0.360000472218843 1105 20-21 0.26280723026494 1105 22-23 0.28434209345505934 1105 24-25 0.2545628951925778 1105 26-27 0.2619968517218396 1105 28-29 0.2564975848344915 1105 30-31 0.19804787322443929 1105 32-33 0.13860585539093506 1105 34-35 0.19012525467760355 1105 36-37 0.2413910812222042 1105 38-39 0.3026369283754846 1105 40-41 0.16074684215903545 1105 42-43 0.10239593592236673 1105 44-45 0.3465705358433979 1105 46-47 0.2029063019015851 1105 48-49 -0.15511419134365667 1105 50-51 0.22698332864818482 1105 52-53 0.2633042819810285 1105 54-55 0.26077540881123795 1105 56-57 0.25684516825170434 1105 58-59 0.10682769963828065 1105 60-61 0.33285035372113825 1105 62-63 0.21579026896992204 1105 64-65 0.3859310477227176 1105 66-67 0.28025217248611867 1105 68-69 0.09956611631611167 1105 70-71 0.09861854296122985 1105 72-73 0.2146669630329079 1105 74-75 0.4154868708972721 1105 76-77 0.33491827167078725 1105 78-79 0.4250292513615257 1105 80-81 0.444614407917598 1105 82-83 0.3883008991947463 1105 84-85 0.2639173541054447 1105 86-87 0.8514236337880341 1105 88-89 1.154799711283733 1105 90-91 1.0930605567229748 1105 92-93 1.2102879537785753 1105 94-95 1.1379965521625088 1105 96-97 1.0122641029160668 1105 98-99 1.2260719681596903 1105 100-101 1.044836332933837 1106 1 -0.01196465414524539 1106 2 0.06309027730558725 1106 3 -0.09120283530056028 1106 4 0.013401465961308645 1106 5 -0.08670413872368954 1106 6 0.024317834173118058 1106 7 0.007979160827773057 1106 8 0.035449368908665235 1106 9 0.036123334249879235 1106 10-11 0.048399336914464186 1106 12-13 -0.1622861964289939 1106 14-15 0.18935087792193173 1106 16-17 0.07417750870982331 1106 18-19 0.07728698110749121 1106 20-21 -0.02229292605890265 1106 22-23 -0.18987174302292686 1106 24-25 -0.18681748071173843 1106 26-27 -0.042136641570770905 1106 28-29 -0.0830232644038773 1106 30-31 -0.005298547686599875 1106 32-33 0.13815869648593093 1106 34-35 0.17414568584057832 1106 36-37 0.1766239310422506 1106 38-39 0.3049854214863288 1106 40-41 0.2904901802184412 1106 42-43 0.1243867019092022 1106 44-45 0.832375967188149 1106 46-47 0.7300812028358834 1106 48-49 0.4963290776424216 1106 50-51 0.9566812237294364 1106 52-53 0.8874308679409637 1106 54-55 1.1460228003842907 1106 56-57 1.0825677116043089 1106 58-59 1.0797218720307633 1106 60-61 1.2208570429275198 1106 62-63 1.1690312621716537 1106 64-65 0.9148146046355059 1106 66-67 1.4065018543852723 1106 68-69 1.4227620300835149 1106 70-71 1.3488094034694633 1106 72-73 1.4801012161321196 1106 74-75 1.4349582781754187 1106 76-77 1.069651248731173 1106 78-79 1.1055651149598837 1106 80-81 1.4013054320205534 1106 82-83 1.3405548709110349 1106 84-85 1.602981955843184 1106 86-87 1.7851532298908417 1106 88-89 1.5777102431483492 1106 90-91 1.5831630760816822 1106 92-93 2.05012145070663 1106 94-95 1.7678453179312221 1106 96-97 1.887368621765816 1106 98-99 2.1039466909575246 1106 100-101 2.1541921332755685 1107 1 0.11067112437541837 1107 2 0.06669786964765478 1107 3 0.1263893949592365 1107 4 0.1051341910724588 1107 5 0.13369068393407701 1107 6 0.06976306249868003 1107 7 0.10530589018008385 1107 8 0.06922209804481838 1107 9 -0.21048243566175984 1107 10-11 -0.062047773471157086 1107 12-13 0.09833998917567044 1107 14-15 0.3538951026063657 1107 16-17 0.17931758242694684 1107 18-19 0.3236952278846985 1107 20-21 0.29365464459288404 1107 22-23 0.15571175149673877 1107 24-25 0.3688598186625285 1107 26-27 0.20924695208314148 1107 28-29 0.06584395905441198 1107 30-31 0.21976788344304765 1107 32-33 0.053320646821902073 1107 34-35 0.2951341959571252 1107 36-37 0.19277424242070396 1107 38-39 0.2459893607684549 1107 40-41 0.17461677391335684 1107 42-43 0.35109853801718316 1107 44-45 0.24066746661234362 1107 46-47 0.25053230095270607 1107 48-49 0.3571572586034222 1107 50-51 0.7811017914780116 1107 52-53 0.9270957494457193 1107 54-55 0.48158851782185863 1107 56-57 0.7435956518107041 1107 58-59 0.8470452099481243 1107 60-61 0.9466856152079437 1107 62-63 0.9471102937865332 1107 64-65 0.9129008663424756 1107 66-67 0.8506419392829514 1107 68-69 0.6039801887952194 1107 70-71 0.4798455054808457 1107 72-73 0.8621761780250594 1107 74-75 0.936829157675124 1107 76-77 1.0035033598062562 1107 78-79 1.0027636406173919 1107 80-81 0.9957475782398468 1107 82-83 1.058840295567144 1107 84-85 1.0505656516128319 1107 86-87 0.9588394215330034 1107 88-89 1.1788681759383088 1107 90-91 1.4875078396688544 1107 92-93 1.6328053801498754 1107 94-95 1.711689118127257 1107 96-97 1.9185996381155093 1107 98-99 1.4291703952244 1107 100-101 1.4277840355724258 1108 1 0.10128225901274135 1108 2 0.08516276536295919 1108 3 -0.04983972235007528 1108 4 0.006606423150252283 1108 5 -0.13470267938563296 1108 6 -0.11183384577580568 1108 7 -0.06518156017643406 1108 8 -0.008410580242525612 1108 9 -0.009184551876145974 1108 10-11 -0.08396148441924112 1108 12-13 0.07925705356245771 1108 14-15 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-1.349253182059595 2316 64-65 -1.4047993993139976 2316 66-67 -2.061386440212541 2316 68-69 -1.8329203786341743 2316 70-71 -1.8041059540245996 2316 72-73 -1.6649071385876972 2316 74-75 -1.5548881388889946 2316 76-77 -1.5302895886595955 2316 78-79 -1.7455649708258996 2316 80-81 -1.574230029567726 2316 82-83 -1.5758539587086418 2316 84-85 -1.6741668860887167 2316 86-87 -1.591980421641388 2316 88-89 -1.9824037647986295 2316 90-91 -2.0602902614090226 2316 92-93 -1.9626784597499594 2316 94-95 -1.6141172817058056 2316 96-97 -1.779210100481727 2316 98-99 -2.3377501566347973 2316 100-101 -2.2311403366034703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 3.0 5 37.0 6 73.0 7 129.0 8 291.0 9 602.0 10 1094.0 11 1899.0 12 2737.0 13 3778.0 14 4960.0 15 7049.0 16 9994.0 17 12783.0 18 10290.0 19 5573.0 20 4307.0 21 4365.0 22 4895.0 23 5116.0 24 5488.0 25 6029.0 26 6891.0 27 7952.0 28 9361.0 29 10848.0 30 12734.0 31 15092.0 32 17717.0 33 21764.0 34 28823.0 35 43194.0 36 45547.0 37 61205.0 38 44788.0 39 3319.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.674768986393616 23.40929951633384 17.71849392920497 15.19743756806757 2 15.236777806299498 26.951598642366157 39.26846543642984 18.5431581149045 3 17.56890372018292 33.51967523268969 33.05809874219218 15.853322304935208 4 10.511441963054867 22.307074337107707 39.08830324291948 28.093180456917942 5 8.762438136169363 43.63866293304681 33.32303259944566 14.275866331338161 6 22.327752583592407 43.32663072930036 17.56177329036061 16.783843396746622 7 21.093950543338753 41.30182634075849 22.13927155528935 15.464951560613407 8 25.874428377209245 34.319947139746915 20.163429451527335 19.6421950315165 9 27.700056092714604 17.48119943336851 22.174686023406824 32.644058450510066 10-11 22.49210899099664 31.89726476712016 25.641976365001952 19.968649876881244 12-13 25.97407227815967 31.514019224735446 27.64589691662626 14.866011580478622 14-15 22.340230835781448 30.96187121493017 30.707552551267792 15.990345398020592 16-17 15.319134270747172 34.307468887557874 31.4284390063033 18.94495783539165 18-19 14.778172328227946 29.710480781114818 40.038433016742246 15.472913873914987 20-21 16.783843396746622 28.117423918313794 40.962417881216545 14.136314803723035 22-23 16.425317695266866 28.76638093008387 40.84028660017042 13.968014774478846 24-25 15.796572865527933 29.632032857137762 40.83243311335879 13.738961163975514 26-27 14.814955665656898 29.74459472789136 41.7439244539466 13.69652515250515 28-29 14.379719576631544 30.046871917494016 41.66335885301661 13.91004965285783 30-31 14.803643688610798 30.296331948826733 40.84353887017131 14.056485492391154 32-33 14.060494566612475 31.34620138235266 40.76692526358822 13.826378787446641 34-35 14.48653774849548 30.897697346529384 40.75456585189622 13.86119905307892 36-37 14.718430541129605 30.726877743736498 40.112565986342815 14.44212572879108 38-39 13.97463931737694 31.877213414779078 40.04337698761676 14.104770280227225 40-41 13.990972875844957 31.69844461557476 40.17438654535429 14.136195963225997 42-43 14.039499910265294 31.840250829303194 38.70596610999454 15.414283150436967 44-45 14.468592833442667 31.814432940684327 38.11892130857648 15.59805291729652 46-47 15.185795233069982 32.45664698668036 37.1292176492399 15.228340131009762 48-49 15.011693904908588 32.928324919426146 35.86736925168516 16.19261192398011 50-51 14.931120047916489 33.106466824486844 35.09514370192902 16.867269425667647 52-53 15.226611809697657 33.73549100909014 34.314127452133846 16.723769729078352 54-55 15.660563018738769 33.29043191390244 33.61082589391822 17.438179173440574 56-57 16.195226414914956 33.54534478005001 32.90467566051548 17.354753144519552 58-59 16.022670013215063 32.90729015145033 33.658956295218815 17.411083540115797 60-61 16.47390738047023 33.70649249403421 33.502205679625035 16.31739444587053 62-63 16.781941948794007 33.489727427435994 34.004306779612676 15.724023844157326 64-65 16.82365496325452 33.61403458733826 33.79823735774792 15.764073091659297 66-67 16.90185201030585 33.74784898700361 33.09886103267638 16.251437970014166 68-69 16.445029139689872 33.523002766606766 33.73037943393894 16.30158865976441 70-71 16.861802762803876 33.68724033351398 33.535956380783965 15.915000522898188 72-73 16.40200887976194 33.32727246798437 33.697460616259285 16.57325803599441 74-75 15.426684920567013 34.516984683836746 32.965046633011035 17.091283762585206 76-77 14.878592548225475 33.61748096175237 34.4585151592938 17.04541133072835 78-79 14.536213076257571 33.4860433720278 34.336228287841195 17.64151526387344 80-81 14.911154844414021 33.98695606704506 33.38764344047993 17.714245648061 82-83 14.948880577947165 33.37587838295536 33.33927537936993 18.335965659727545 84-85 14.465401330777466 33.30722800646018 33.03829164205675 19.189079020705606 86-87 14.364013196048791 33.53001435593204 32.96766112394589 19.138311324073282 88-89 14.600743466149474 32.89219740832644 32.96385822804066 19.543200897483434 90-91 14.603530014392458 32.47275713591304 33.040492599947946 19.883220249746557 92-93 15.041339796848503 33.07207516629211 32.58814343489323 19.29844160196615 94-95 14.664560813059143 32.18854282536151 33.000936463116666 20.145959898462678 96-97 14.745372351045322 32.10000665506784 32.70300333704116 20.451617656845688 98-99 14.885406825082745 32.3500365438437 32.17997397365278 20.58458265742078 100-101 14.623748184525862 32.000836722614565 32.86335036095548 20.51206473190409 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5028.0 1 3339.0 2 1942.5 3 2248.5 4 1903.5 5 1901.0 6 3158.0 7 6073.5 8 8599.5 9 8857.0 10 6890.0 11 4791.0 12 4360.0 13 4172.0 14 3746.5 15 3326.5 16 3038.5 17 2845.0 18 2707.0 19 2557.5 20 2509.5 21 2586.0 22 2712.0 23 2942.5 24 3133.0 25 3468.0 26 4383.5 27 5317.5 28 5527.0 29 6025.0 30 7234.5 31 8356.0 32 10198.5 33 12713.5 34 14627.0 35 16394.0 36 19231.5 37 21175.0 38 20379.0 39 18770.5 40 16914.5 41 14790.0 42 12787.0 43 12247.5 44 12360.0 45 11420.0 46 9760.5 47 9463.5 48 11420.5 49 10069.0 50 6567.5 51 4348.0 52 2734.0 53 2217.0 54 1980.0 55 1856.5 56 1851.0 57 1715.0 58 1597.5 59 1514.0 60 1366.0 61 1301.5 62 1113.0 63 650.0 64 340.5 65 274.5 66 270.5 67 232.0 68 164.5 69 107.5 70 85.0 71 74.5 72 73.5 73 101.5 74 97.0 75 61.5 76 40.5 77 30.5 78 28.5 79 24.0 80 14.5 81 6.0 82 3.5 83 3.0 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04611011285093599 2 0.0 3 0.0 4 0.0 5 0.011884049703849481 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005704343857847751 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0015449264615004328 24-25 3.5652149111548443E-4 26-27 0.002020288449654412 28-29 0.002614490934846886 30-31 0.0010695644733464532 32-33 0.0 34-35 0.0 36-37 2.376809940769896E-4 38-39 4.753619881539792E-4 40-41 0.0 42-43 0.011289847218657008 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.5652149111548443E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.5652149111548443E-4 84-85 1.188404970384948E-4 86-87 0.0 88-89 0.0 90-91 0.006060865348963235 92-93 0.0027333314318853806 94-95 0.0 96-97 0.0 98-99 0.0010695644733464532 100-101 0.010220282745310554 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 420732.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.283467668989672 #Duplication Level Percentage of deduplicated Percentage of total 1 76.50619694940104 23.168729417945425 2 10.092512920362836 6.11272577445337 3 3.7954876738007783 3.44821584772784 4 1.9484411246457438 2.3602221521255697 5 1.2247083949587079 1.854420854133613 6 0.8227340901356172 1.4949144731278559 7 0.600551681403021 1.2730751199128036 8 0.5186565194764682 1.2565374351101066 9 0.4234169969768474 1.1540281444604148 >10 3.3393049003685156 20.64842759898432 >50 0.45725563552574766 9.573694347218098 >100 0.2549928156804514 13.495264881439178 >500 0.007083133768901427 1.2965445305799608 >1k 0.0055091040424788875 3.3874609215317983 >5k 0.0015740297264225393 3.8207546635528224 >10k+ 0.0015740297264225393 5.654983837696826 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12782 3.0380384662920816 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10945 2.6014184801726516 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8765 2.083273913084814 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7266 1.7269901029634067 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3193 0.7589154140878278 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2579 0.6129792837245562 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2133 0.5069735603662189 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1754 0.4168924636110398 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1742 0.4140402916821159 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1530 0.36365192093779414 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1282 0.3047070344067007 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 801 0.1903824762556687 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 763 0.18135059848074309 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 663 0.1575824990730441 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 594 0.14118251048173186 No Hit GTACATGGGAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG 540 0.1283477368015744 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 540 0.1283477368015744 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 521 0.12383179791411159 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 516 0.12264339294372664 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 502 0.11931585902664879 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 479 0.11384919616287803 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 477 0.11337383417472406 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 471 0.11194774821026211 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 448 0.10648108534649135 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 447 0.10624340435241436 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 446 0.10600572335833737 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 424 0.10077674148864361 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1884049703849482E-4 0.0 12-13 0.0 0.0 0.0 4.7536198815397926E-4 0.0 14-15 0.0 0.0 0.0 4.7536198815397926E-4 0.0 16-17 0.0 0.0 0.0 7.130429822309689E-4 0.0 18-19 0.0 0.0 0.0 7.130429822309689E-4 0.0 20-21 0.0 0.0 0.0 8.318834792694637E-4 0.0 22-23 0.0 0.0 0.0 0.0019014479526159168 0.0 24-25 0.0 0.0 0.0 0.004515938887462803 0.0 26-27 0.0 0.0 0.0 0.008437675289733132 0.0 28-29 0.0 0.0 0.0 0.0160434671001968 0.0 30-31 0.0 0.0 0.0 0.03624635159674092 0.0 32-33 0.0 0.0 0.0 0.05846952454293945 0.0 34-35 0.0 0.0 0.0 0.07926661152467604 0.0 36-37 0.0 0.0 0.0 0.1077883308139148 0.0 38-39 2.3768099407698963E-4 0.0 0.0 0.14593613036327163 0.0 40-41 2.3768099407698963E-4 0.0 0.0 0.19525493663424698 0.0 42-43 2.3768099407698963E-4 0.0 0.0 0.2473070743371077 0.0 44-45 2.3768099407698963E-4 0.0 0.0 0.3138577526786648 0.0 46-47 2.3768099407698963E-4 0.0 0.0 0.4052460949012673 0.0 48-49 2.3768099407698963E-4 0.0 0.0 0.504121388437295 0.0 50-51 2.3768099407698963E-4 0.0 0.0 0.6317560822566384 0.0 52-53 2.3768099407698963E-4 0.0 0.0 0.779355979578449 0.0 54-55 2.3768099407698963E-4 0.0 0.0 0.9355123926870312 0.0 56-57 2.3768099407698963E-4 0.0 0.0 1.1509702138178222 0.0 58-59 2.3768099407698963E-4 0.0 0.0 1.3768859986880009 0.0 60-61 2.3768099407698963E-4 0.0 0.0 1.6313235028474182 0.0 62-63 2.3768099407698963E-4 0.0 0.0 1.8832653565690274 0.0 64-65 2.3768099407698963E-4 0.0 0.0 2.153627487331603 0.0 66-67 2.3768099407698963E-4 0.0 0.0 2.4811518971696946 0.0 68-69 2.3768099407698963E-4 0.0 0.0 2.858589315763954 0.0 70-71 2.3768099407698963E-4 0.0 0.0 3.3087571185457723 0.0 72-73 2.3768099407698963E-4 0.0 0.0 3.7175684283581947 0.0 74-75 2.3768099407698963E-4 0.0 0.0 4.165359421199243 0.0 76-77 2.3768099407698963E-4 0.0 0.0 4.605306941235751 0.0 78-79 2.3768099407698963E-4 0.0 0.0 5.135692079518553 0.0 80-81 2.3768099407698963E-4 0.0 0.0 5.669523592215472 0.0 82-83 2.3768099407698963E-4 0.0 0.0 6.2527927516804045 0.0 84-85 2.3768099407698963E-4 0.0 0.0 6.8636329064582675 0.0 86-87 2.3768099407698963E-4 0.0 0.0 7.486119429945903 0.0 88-89 2.3768099407698963E-4 0.0 0.0 8.172423300343212 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGCA 25 0.005018274 57.002277 4 TATCACG 25 0.005018274 57.002277 2 AACAGCG 35 2.7837208E-4 54.294342 7 CAGCGCA 40 5.3800445E-4 47.507545 9 ACAGCGC 40 5.3800445E-4 47.507545 8 GTATGGT 65 2.5720692E-6 43.8792 1 CATGGGT 205 0.0 39.391823 4 GGTACTC 75 6.9042962E-6 38.00604 8 GGTATCA 5815 0.0 36.132114 1 TGGTACT 85 1.6279819E-5 33.53474 7 CATGAGA 100 1.2961937E-6 33.25133 4 GTATCAA 8595 0.0 31.082167 1 ATGGGTA 155 1.3515091E-9 30.646389 5 TGGGTAT 80 4.490925E-4 29.685158 6 CTGGTCG 155 4.5658453E-8 27.58503 9 CCGCACC 35 0.008383814 27.147171 90-91 CAGAGTC 70 0.008383992 27.143942 4 CCGGCTA 35 0.008393542 27.140717 32-33 GTACATG 4940 0.0 26.173561 1 TAGGACA 200 7.1486284E-10 26.126045 4 >>END_MODULE