FastQCFastQC Report
Thu 26 May 2016
SRR937185_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937185_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462398
Sequences flagged as poor quality0
Sequence length101
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155003.352090623229339No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116962.5294227051155067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93582.0237976807858167No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62991.3622463764981683No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31000.6704181246458678No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25740.5566633073672463No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23390.505841288240866No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21340.46150718644976835No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16120.3486174248158513No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15780.34126445183586435No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13300.2876310018641949No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11300.24437821962897763No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.1894471861902517No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8220.177768934986743No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA8000.17301112894086912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6810.14727572351091484No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6710.14511308439915396No Hit
GTACATGGGAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG6350.13732758359681485No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5720.12370295719272142No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA5470.11829635941331927No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5040.10899701123274755No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.1083482194992193No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.10791569167686711No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4810.10402294127569757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4670.10099524651923233No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTA250.005005089657.0410585
AGATAGT405.3829607E-447.5033076
TCGACCG250.001615959137.99852818-19
GGTATCA57150.037.694391
GTATGGT550.00256348234.5853421
ATCCATC550.002577152834.547867
GTATCAA88250.033.4096371
GGGATGC1001.2962537E-633.252317
GGTACTC753.060701E-431.6929133
TAGATAG753.062649E-431.6894745
TAGGCAT753.062649E-431.6894745
GTTCGAC300.00394920631.6688716-17
CGACCGT300.00395130231.66543818-19
GACTCCC2100.031.6620049
GTACAGG1102.7043316E-630.2621731
GTCACTA953.4590026E-530.0346371
GGGAACG804.4876893E-429.6895667
GCTTTGC650.005807646629.264521
GTACATG59900.028.5805421
CATGGGT3200.028.2265014
Produced by FastQC (version 0.11.5)