##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937185_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 462398 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.975306986621913 33.0 31.0 34.0 31.0 34.0 2 31.990034558973004 33.0 31.0 34.0 30.0 34.0 3 31.94997599470586 33.0 31.0 34.0 30.0 34.0 4 35.68384378825168 37.0 35.0 37.0 35.0 37.0 5 35.725980648705224 37.0 35.0 37.0 35.0 37.0 6 35.88284551403769 37.0 35.0 37.0 35.0 37.0 7 35.84050536550764 37.0 35.0 37.0 35.0 37.0 8 35.85626019143682 37.0 35.0 37.0 35.0 37.0 9 37.468092422545084 39.0 37.0 39.0 35.0 39.0 10-11 37.412294170822534 39.0 37.0 39.0 35.0 39.0 12-13 37.32280524569743 39.0 37.0 39.0 35.0 39.0 14-15 38.28964333755769 40.0 38.0 41.0 34.0 41.0 16-17 38.32902283314374 40.0 38.0 41.0 34.5 41.0 18-19 38.49753675405171 40.0 38.0 41.0 34.5 41.0 20-21 38.577710111202904 40.0 38.0 41.0 35.0 41.0 22-23 38.45524959017989 40.0 38.0 41.0 35.0 41.0 24-25 38.30747970363194 40.0 38.0 41.0 34.0 41.0 26-27 38.08623739722057 40.0 37.0 41.0 34.0 41.0 28-29 37.8466137396788 40.0 37.0 41.0 34.0 41.0 30-31 37.47271073836824 39.0 36.0 41.0 34.0 41.0 32-33 36.97467873995995 38.5 35.0 41.0 33.0 41.0 34-35 36.6441193517273 38.0 35.0 40.0 33.0 41.0 36-37 36.38415715465897 38.0 35.0 40.0 33.0 41.0 38-39 36.19278521965926 38.0 35.0 41.0 32.5 41.0 40-41 35.878122310217606 38.0 35.0 40.5 31.5 41.0 42-43 35.385559626122955 38.0 35.0 40.0 30.0 41.0 44-45 34.76980004238773 38.0 35.0 40.0 25.5 41.0 46-47 33.95725219399738 38.0 35.0 40.0 20.0 41.0 48-49 33.28014610789839 38.0 34.0 40.0 4.5 41.0 50-51 32.19834644613515 36.0 32.0 39.5 2.0 40.5 52-53 32.30299871539236 37.0 33.0 39.5 2.0 40.5 54-55 32.585551840622145 37.0 33.0 40.0 2.0 41.0 56-57 32.33082323020428 37.0 33.0 40.0 2.0 41.0 58-59 32.10867369668554 36.0 33.0 40.0 2.0 41.0 60-61 31.8450036548601 36.0 33.0 40.0 2.0 41.0 62-63 31.527532558531828 35.5 33.0 40.0 2.0 41.0 64-65 31.1734220303721 35.0 32.5 39.5 2.0 41.0 66-67 30.779319979757698 35.0 31.5 39.0 2.0 41.0 68-69 30.301641659349738 35.0 31.0 39.0 2.0 40.5 70-71 29.863211994861572 35.0 31.0 38.0 2.0 40.0 72-73 29.438238270926778 35.0 30.0 37.5 2.0 40.0 74-75 28.98200467995104 35.0 29.0 37.0 2.0 39.0 76-77 28.542754293919955 35.0 29.0 36.5 2.0 39.0 78-79 28.046403747421053 35.0 27.5 36.0 2.0 38.5 80-81 27.530931145895963 34.5 26.5 35.5 2.0 37.5 82-83 27.023183491278076 34.0 25.5 35.0 2.0 37.0 84-85 26.45577403016449 34.0 23.0 35.0 2.0 36.5 86-87 25.977537748865696 34.0 19.5 35.0 2.0 36.0 88-89 25.488414742278295 33.5 12.0 35.0 2.0 36.0 90-91 24.958810375477402 33.0 2.0 35.0 2.0 35.0 92-93 24.441030238020062 33.0 2.0 35.0 2.0 35.0 94-95 23.855779004234446 33.0 2.0 35.0 2.0 35.0 96-97 23.24012971509392 32.0 2.0 35.0 2.0 35.0 98-99 22.63303907023819 32.0 2.0 35.0 2.0 35.0 100-101 21.215982768091557 29.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07166589114523347 1101 2 -0.0651436633703888 1101 3 0.17315608959883733 1101 4 0.020446146748788863 1101 5 0.028471666225925674 1101 6 -0.05119937881716652 1101 7 5.640770649790738E-4 1101 8 0.11156590799602384 1101 9 -0.08332169403570333 1101 10-11 0.054798337987669754 1101 12-13 -0.06677133630589083 1101 14-15 0.0067154525608685844 1101 16-17 0.15424536630064267 1101 18-19 0.23128774047307843 1101 20-21 0.023638343652351068 1101 22-23 0.2299944802827838 1101 24-25 0.3331461217502465 1101 26-27 0.3109144494552041 1101 28-29 0.21571631478238373 1101 30-31 0.28104638873013954 1101 32-33 0.1565666111284827 1101 34-35 0.349126207064387 1101 36-37 0.708355487922006 1101 38-39 0.6008060036854985 1101 40-41 0.8310090363179583 1101 42-43 0.9079653060668846 1101 44-45 1.0973463812642947 1101 46-47 1.5209765004622042 1101 48-49 1.2568783212589594 1101 50-51 1.1682882982675267 1101 52-53 1.0863288877326696 1101 54-55 1.1367908248491645 1101 56-57 1.284448026166224 1101 58-59 1.5995096062905212 1101 60-61 1.5132642852487486 1101 62-63 1.6519445814466778 1101 64-65 1.7460221682581114 1101 66-67 1.8406202932373503 1101 68-69 1.8712695466871416 1101 70-71 1.6633956134166503 1101 72-73 1.4701134616251785 1101 74-75 1.3568080325210694 1101 76-77 1.7404937614349016 1101 78-79 1.5921883352906576 1101 80-81 1.6809475478862943 1101 82-83 1.4871338299270178 1101 84-85 1.3297120352432898 1101 86-87 1.6107238927374965 1101 88-89 1.7336452485195082 1101 90-91 1.8418059081931695 1101 92-93 1.7472999699296707 1101 94-95 1.8542992665109281 1101 96-97 2.011337740432726 1101 98-99 2.2283353417804044 1101 100-101 2.3635836106621717 1102 1 0.2814873197166641 1102 2 0.1492981944538201 1102 3 0.17800363672579422 1102 4 0.022334064077888627 1102 5 0.32290869779530595 1102 6 0.17667792075130961 1102 7 0.1825706634833253 1102 8 0.17508084099624455 1102 9 0.24491331618460066 1102 10-11 0.23848701616164902 1102 12-13 0.22743502867253085 1102 14-15 0.32517729675801377 1102 16-17 0.3088877975974924 1102 18-19 0.4185570738821127 1102 20-21 0.17214504362964078 1102 22-23 0.0730205420588419 1102 24-25 0.20117644198644768 1102 26-27 0.13321245649584768 1102 28-29 0.09860156347644988 1102 30-31 0.21587775370856122 1102 32-33 0.15061541308170234 1102 34-35 0.24899561428673422 1102 36-37 0.29097671208734965 1102 38-39 0.40255694054673796 1102 40-41 0.3667836502175774 1102 42-43 0.3778721877384683 1102 44-45 0.24807812666515616 1102 46-47 0.33502019226325075 1102 48-49 0.47408462945791285 1102 50-51 0.3871341136207036 1102 52-53 0.6571967619093684 1102 54-55 0.7594380256213711 1102 56-57 0.9529375503542781 1102 58-59 0.8067688613438939 1102 60-61 0.7239445032819098 1102 62-63 0.9165191612786074 1102 64-65 1.201223167576753 1102 66-67 1.1217070522652932 1102 68-69 1.1087354790060182 1102 70-71 1.0734108019244886 1102 72-73 1.1174668286476646 1102 74-75 1.0309648574131849 1102 76-77 0.8139273578464383 1102 78-79 0.8877112741959508 1102 80-81 1.0531121231758682 1102 82-83 0.9275331315395547 1102 84-85 0.9510781492564604 1102 86-87 1.360205780087032 1102 88-89 1.2454234395256378 1102 90-91 1.000085774193625 1102 92-93 0.7790396928458634 1102 94-95 1.0017023155683873 1102 96-97 0.793655904184714 1102 98-99 1.064629487817193 1102 100-101 1.550884882522265 1103 1 0.06800288748223338 1103 2 -0.005100103326828531 1103 3 0.04564396208671084 1103 4 -0.07008944378679871 1103 5 0.06812120587546389 1103 6 -0.008579836197625923 1103 7 -0.13452155802065846 1103 8 -0.02104472461461171 1103 9 0.0865624758484671 1103 10-11 0.013515296704632362 1103 12-13 0.018418248883691035 1103 14-15 -0.0022440563986378947 1103 16-17 0.004309716364993221 1103 18-19 0.17792668711201998 1103 20-21 0.13726570727971676 1103 22-23 0.1599024101907105 1103 24-25 0.1918977305018572 1103 26-27 0.07086395940471135 1103 28-29 0.14993074899681602 1103 30-31 0.0246608823446266 1103 32-33 0.11629832086018865 1103 34-35 0.09840845634663964 1103 36-37 0.11561714518366273 1103 38-39 0.2687596716391667 1103 40-41 0.16743612274505182 1103 42-43 0.28473493283650697 1103 44-45 0.19251022642813354 1103 46-47 0.2748870043727081 1103 48-49 0.06615463053527293 1103 50-51 0.25728938726862083 1103 52-53 0.5068318082355958 1103 54-55 0.3705300585884075 1103 56-57 0.44163543335363187 1103 58-59 0.3590878658687835 1103 60-61 0.45888848087294676 1103 62-63 0.388208317710415 1103 64-65 0.5815830038189453 1103 66-67 0.6989264015434635 1103 68-69 0.6044395298571281 1103 70-71 0.8154350154560532 1103 72-73 0.5696828111945251 1103 74-75 0.5794592551722921 1103 76-77 0.29892856986970884 1103 78-79 0.19485143795376203 1103 80-81 0.16099902793777332 1103 82-83 0.2923016350948231 1103 84-85 0.40294735847860963 1103 86-87 0.49823503024863314 1103 88-89 0.5020592669335251 1103 90-91 0.11504618143344203 1103 92-93 0.05058677321647309 1103 94-95 -0.1687182565065939 1103 96-97 -0.04140456230957312 1103 98-99 0.22984509329015523 1103 100-101 0.2303047273832881 1104 1 0.06239454092324692 1104 2 0.007703668160672805 1104 3 0.10414152789688913 1104 4 0.19382427807938996 1104 5 0.11601481135590319 1104 6 -0.0028944795067857854 1104 7 0.01036128613092302 1104 8 0.02311221533757646 1104 9 0.22994194829558268 1104 10-11 -0.022942304195382235 1104 12-13 0.218870599701674 1104 14-15 0.2522191829823299 1104 16-17 0.23128368694042933 1104 18-19 0.14739384396492028 1104 20-21 0.1167224175408137 1104 22-23 0.15145975595281413 1104 24-25 0.27412111345650203 1104 26-27 0.11435794256961884 1104 28-29 0.07764836958463661 1104 30-31 0.03469563735196601 1104 32-33 0.0174240080919148 1104 34-35 -0.04182866789414419 1104 36-37 -0.14114300508654054 1104 38-39 -0.24658376006623683 1104 40-41 -0.4166087172412887 1104 42-43 -0.25015233102698886 1104 44-45 -0.07506649910106233 1104 46-47 -0.034265147558237175 1104 48-49 0.2412790186395526 1104 50-51 0.25396623266575347 1104 52-53 0.19851504882193893 1104 54-55 0.42577143512777127 1104 56-57 0.2760519309328302 1104 58-59 0.08163040559596269 1104 60-61 0.04737981890724896 1104 62-63 0.10630603352293733 1104 64-65 0.1034438364658854 1104 66-67 0.009262165718350701 1104 68-69 0.05111503594762823 1104 70-71 0.2607377322395763 1104 72-73 0.4010406672324578 1104 74-75 0.598089010547934 1104 76-77 0.3993313663419755 1104 78-79 0.32107104406367526 1104 80-81 0.5459994632124285 1104 82-83 0.48785379774022175 1104 84-85 0.6925422851850556 1104 86-87 0.9832331217366708 1104 88-89 0.6772054187707184 1104 90-91 0.6530155247044327 1104 92-93 0.9287600928888793 1104 94-95 0.8562280038144365 1104 96-97 0.8911613483184944 1104 98-99 0.9454550470171093 1104 100-101 0.8524294034384958 1105 1 0.2822886017679487 1105 2 0.19777394570106566 1105 3 0.05761916636960862 1105 4 0.13884264976068295 1105 5 0.21661373898338354 1105 6 0.1861228969666442 1105 7 0.144007605123889 1105 8 0.17816632075027883 1105 9 0.280419900475124 1105 10-11 0.3650932059748513 1105 12-13 0.31476933369631155 1105 14-15 0.37159364436214304 1105 16-17 0.21922941205392021 1105 18-19 0.2950019195718738 1105 20-21 0.1801651155637387 1105 22-23 0.3228356308162361 1105 24-25 0.24303889702763826 1105 26-27 0.19047511747740486 1105 28-29 0.3225145754267942 1105 30-31 0.21386911001439302 1105 32-33 0.31015574548684555 1105 34-35 0.5277547510775449 1105 36-37 0.49128634777594016 1105 38-39 0.3887230416025389 1105 40-41 0.2047705831564386 1105 42-43 0.1442023884578134 1105 44-45 0.31916562231430134 1105 46-47 0.16175654508840154 1105 48-49 0.14814955868404667 1105 50-51 -0.18276414893876236 1105 52-53 -0.04480245493713397 1105 54-55 -0.07417267457586973 1105 56-57 0.16035901746183612 1105 58-59 0.08492613016858286 1105 60-61 0.12301922192676429 1105 62-63 0.20442415315702078 1105 64-65 0.4132257200770475 1105 66-67 0.33030143676464974 1105 68-69 0.3521149313786829 1105 70-71 0.20272721044055686 1105 72-73 0.2484655669003608 1105 74-75 0.5204285422954236 1105 76-77 0.4865166228423803 1105 78-79 0.6669562562124263 1105 80-81 0.5629432375742915 1105 82-83 0.4062774029167464 1105 84-85 0.32616516631180303 1105 86-87 0.012112178741165991 1105 88-89 0.46085047572473314 1105 90-91 0.6081428283762413 1105 92-93 0.5060671902353526 1105 94-95 0.6721524112871506 1105 96-97 0.9025233046952188 1105 98-99 0.8489631547159071 1105 100-101 1.0472954289893757 1106 1 -0.44996188347058563 1106 2 -0.32613829488294854 1106 3 -0.2948329580714848 1106 4 -0.2829887622638054 1106 5 -0.23407659632233901 1106 6 -0.285143909574451 1106 7 -0.19834684296147742 1106 8 -0.21182008471268432 1106 9 -0.196021474066157 1106 10-11 -0.3640513597464903 1106 12-13 -0.30028131184774054 1106 14-15 -0.26403936600271294 1106 16-17 -0.5311674226978056 1106 18-19 -0.6042158051898241 1106 20-21 -0.5023042181467829 1106 22-23 -0.46420555355868487 1106 24-25 -0.5181667733873496 1106 26-27 -0.37298701034267623 1106 28-29 -0.3068574826918109 1106 30-31 0.04853242912454192 1106 32-33 0.24420063103341505 1106 34-35 0.09985687512573094 1106 36-37 -0.3491173235348697 1106 38-39 -0.30338033974387457 1106 40-41 0.11921143906219811 1106 42-43 0.12670040428405827 1106 44-45 0.026248533274014108 1106 46-47 0.30511408141253327 1106 48-49 0.5932586685954888 1106 50-51 0.6148226242839314 1106 52-53 0.3844568392470791 1106 54-55 0.6134594399639965 1106 56-57 0.48996956933716973 1106 58-59 0.6456339890682834 1106 60-61 0.7222161226866426 1106 62-63 0.9804875524199304 1106 64-65 0.7296975055191091 1106 66-67 0.6707168952463078 1106 68-69 0.4638298075741005 1106 70-71 0.6619573510222132 1106 72-73 0.6172507810732526 1106 74-75 0.34065257712258656 1106 76-77 0.621648678446391 1106 78-79 0.7479321553771072 1106 80-81 0.8737670036539562 1106 82-83 0.7523624905740043 1106 84-85 0.7565913904094934 1106 86-87 0.829043663846111 1106 88-89 0.5749326087311672 1106 90-91 0.736866592098476 1106 92-93 0.9213527933848837 1106 94-95 0.7637387287392343 1106 96-97 1.0342999456658504 1106 98-99 0.9264579094248795 1106 100-101 0.543458704879896 1107 1 0.017545012024353213 1107 2 0.08510136187463502 1107 3 -0.0042644628217161085 1107 4 0.20610103240368005 1107 5 0.11400032675458505 1107 6 0.16669488907709962 1107 7 -0.05860580710490382 1107 8 0.04433989077000433 1107 9 0.007441596727588262 1107 10-11 0.006784527473861601 1107 12-13 0.031238761704202034 1107 14-15 0.17760942345483954 1107 16-17 0.18984085189612898 1107 18-19 0.3081922548775964 1107 20-21 0.3270092970233023 1107 22-23 0.21580900359730038 1107 24-25 0.4382805143846369 1107 26-27 0.30438044292119315 1107 28-29 0.28262305668911836 1107 30-31 0.3511718713556178 1107 32-33 0.35031846738034034 1107 34-35 0.3579322658704456 1107 36-37 0.30723802430454583 1107 38-39 0.33936406724356516 1107 40-41 0.4468317271406548 1107 42-43 0.3504400610416383 1107 44-45 0.17900290042080513 1107 46-47 -0.27172471723901737 1107 48-49 -0.3552281533475181 1107 50-51 -0.1652838728046433 1107 52-53 -0.047243283339497566 1107 54-55 0.25198610254444986 1107 56-57 0.38284422456242595 1107 58-59 0.3840878658687821 1107 60-61 0.4251888471733132 1107 62-63 0.49535117485326907 1107 64-65 0.5759969232328679 1107 66-67 0.6564355590526212 1107 68-69 0.8397875152051135 1107 70-71 0.885444172965208 1107 72-73 0.9599300639417798 1107 74-75 0.9680123687254039 1107 76-77 0.796181317122457 1107 78-79 0.898514441616765 1107 80-81 0.9033525077912508 1107 82-83 0.6776038328970202 1107 84-85 0.8007953438265929 1107 86-87 1.0143064588200623 1107 88-89 1.2262350911093485 1107 90-91 1.4881231045103682 1107 92-93 1.4210080186377212 1107 94-95 1.2143861390977975 1107 96-97 1.4692639358589226 1107 98-99 1.021374397319459 1107 100-101 1.062310221888783 1108 1 -0.09899932150471003 1108 2 -0.23807832103787163 1108 3 -0.3913020435615877 1108 4 -0.2875278577900957 1108 5 -0.2741529322002023 1108 6 -0.3471630087502646 1108 7 -0.17749893687589946 1108 8 -0.1859008608447965 1108 9 0.070477415870279 1108 10-11 -0.07669315896183804 1108 12-13 -0.051703105588806864 1108 14-15 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-1.441510218065158 2316 100-101 -2.0608308037522427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1686.0 3 236.0 4 142.0 5 174.0 6 197.0 7 239.0 8 314.0 9 399.0 10 606.0 11 1212.0 12 2166.0 13 3421.0 14 4218.0 15 6058.0 16 9114.0 17 13207.0 18 9964.0 19 5102.0 20 4598.0 21 4257.0 22 4585.0 23 4773.0 24 5133.0 25 5508.0 26 6052.0 27 6877.0 28 7978.0 29 9103.0 30 10899.0 31 13028.0 32 15959.0 33 20629.0 34 28753.0 35 50398.0 36 53305.0 37 70003.0 38 70492.0 39 11613.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.99261050068469 22.262033164854195 16.490628031485127 16.25472830297598 2 14.474072402901669 25.21150775745375 41.1288223248065 19.185597514838083 3 17.059789529402323 34.25147570216523 31.931786874114632 16.756947894317815 4 9.797813259284972 20.69572197153766 39.98047552933009 29.525989239847277 5 8.813137283396848 43.17574001331774 32.95708172193675 15.054040981348649 6 22.03961769359138 42.467870223878975 18.719143494939424 16.773368587590213 7 21.61592165753784 40.961160397868035 22.20201621730583 15.220901727288293 8 25.551902097932413 33.35818744188365 20.564029313398027 20.52588114678591 9 28.78352428139806 16.788908106514775 22.02751732837432 32.400050283712844 10-11 22.1622998347197 31.458530901996912 25.81678272414447 20.562386539138917 12-13 25.763728802170206 31.16085043845246 27.652146959336797 15.423273800040535 14-15 22.44021368123354 30.375242450182583 30.611462069413893 16.57308179916998 16-17 15.506061949252375 34.294474072934136 30.8257190944628 19.373744883350692 18-19 14.727092063017347 29.979075848154157 39.40242096012708 15.891411128701414 20-21 17.008715666418514 28.37245793062793 39.96072911214634 14.65809729080721 22-23 16.52256081108172 29.167321364907668 39.841051909533526 14.469065914477087 24-25 15.707012208005253 30.11618571077202 39.79569171292388 14.381110368298843 26-27 14.606018309810311 30.246514894059718 40.791669556472584 14.355797239657383 28-29 14.34318082562017 30.524516863844088 40.670023938715154 14.462278371820588 30-31 14.63765681307069 30.989785135611132 39.839054921830545 14.533503129487633 32-33 13.878532628722292 31.812841666269275 39.92916318046048 14.379462524547948 34-35 14.368411470975795 31.65181357930508 39.60052708232193 14.379247867397185 36-37 14.47381110534397 31.440760337778606 38.96741951318357 15.118009043693851 38-39 13.817504534432432 32.56155884793995 38.97163189726721 14.649304720360416 40-41 13.66734674197697 32.48022346053956 39.23095509227607 14.6214747052074 42-43 13.684087348281443 32.66957480881457 37.77897686271706 15.867360980186925 44-45 14.056309332870953 32.5288265637869 37.42565780918115 15.989206294160995 46-47 14.698784997816617 33.09361248874446 36.58843683733187 15.619165676107052 48-49 14.46934953922444 33.40807029102983 35.57182983814337 16.550750331602355 50-51 14.402810050634669 33.597055559409036 35.089976243323854 16.910158146632448 52-53 14.736926535399103 34.349345432552134 34.2104977920949 16.70323023995387 54-55 15.112447840459545 33.77759713867664 33.83753319243483 17.272421828428982 56-57 15.568255687973998 34.011917659804986 33.085265438786564 17.33456121343445 58-59 15.43632885366588 33.38670225557719 33.80625131346045 17.37071757729647 60-61 15.917238420561688 33.964293955713586 33.851189063927016 16.26727855979771 62-63 15.992411857177371 33.92432875162916 34.33612959213191 15.747129799061558 64-65 16.181366510768257 33.879356948854706 34.19722069646179 15.742055843915248 66-67 16.062973356592593 34.137583565384155 33.64220471758422 16.157238360439035 68-69 15.77891918888789 33.89416800555562 34.177149363671134 16.14976344188536 70-71 16.190644379823965 33.78931219636922 34.1315672007246 15.888476223082224 72-73 15.648422761302793 33.515306437578495 34.3621423702954 16.474128430823313 74-75 14.778028902723634 34.461048065025714 33.87575599323046 16.885167039020192 76-77 14.39284924821318 33.74827169215966 34.8588313821695 17.000047677457665 78-79 14.11748516347001 33.477734640385485 34.92796007329143 17.47682012285308 80-81 14.556442264147673 33.77630241794816 34.04054543051351 17.626709887390657 82-83 14.76555956123213 33.08876673200724 34.049153877578 18.096519829182636 84-85 14.4943724059928 32.77232538966308 33.78549815230309 18.947804052041025 86-87 14.395776317300587 32.87915526346012 33.927816889963665 18.797251529275634 88-89 14.884116544408446 32.12014172562872 33.780758687195934 19.2149830427669 90-91 14.910060523059654 31.505578826247028 34.18036001794239 19.40400063275093 92-93 15.500530398497764 31.669937186514986 34.00358222151912 18.825950193468135 94-95 15.252514635977796 30.58922068226465 34.46776834251276 19.690496339244792 96-97 15.356471128677171 30.511207031046855 34.25338464472065 19.878937195555324 98-99 15.465146264982435 30.64077476341241 34.1439180682057 19.75016090339945 100-101 15.85185120987312 29.45057373639326 34.97625320668377 19.72132184704985 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4216.0 1 2867.0 2 2027.0 3 2428.5 4 1953.5 5 2040.0 6 3797.5 7 7695.5 8 11396.0 9 11605.5 10 8331.5 11 5331.5 12 4752.0 13 4596.0 14 4198.5 15 3703.5 16 3301.5 17 3069.0 18 2891.5 19 2831.0 20 2785.5 21 2867.0 22 2975.5 23 3104.5 24 3271.0 25 3650.5 26 4607.0 27 5522.5 28 5623.5 29 6033.5 30 7564.5 31 9027.0 32 10917.0 33 13725.5 34 15949.5 35 17731.5 36 20839.0 37 23277.0 38 22538.0 39 20655.0 40 18283.0 41 15736.0 42 13571.5 43 13206.5 44 13253.5 45 12232.0 46 10721.5 47 10201.5 48 12258.5 49 10933.0 50 7239.5 51 4983.5 52 3326.0 53 2744.5 54 2445.0 55 2270.0 56 2219.5 57 1989.0 58 1790.5 59 1672.0 60 1546.5 61 1559.0 62 1342.5 63 774.5 64 406.5 65 365.5 66 341.0 67 257.0 68 187.0 69 138.5 70 129.5 71 96.0 72 86.5 73 132.0 74 125.5 75 73.0 76 45.0 77 38.5 78 30.0 79 21.5 80 13.5 81 7.5 82 4.0 83 3.5 84 3.5 85 1.5 86 0.5 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34796863308232306 2 0.30752728169239485 3 0.3094736568929796 4 0.31098750427121225 5 0.2939026552883014 6 0.21366874424197335 7 0.22469820371195376 8 0.22469820371195376 9 0.21972413375490377 10-11 0.22934787780223964 12-13 0.22913161389106354 14-15 0.2082621464625712 16-17 0.22415754393401355 18-19 0.20966786188521577 20-21 0.21356061228638532 22-23 0.2139931401087375 24-25 0.20296368063875708 26-27 0.2175614946431429 28-29 0.2187509461546114 30-31 0.22934787780223964 32-33 0.22837469020194723 34-35 0.21410127206432555 36-37 0.21010038970756795 38-39 0.20090917348258427 40-41 0.2010173054381723 42-43 0.20220675694964077 44-45 0.22015666157725594 46-47 0.20728895886227883 48-49 0.2166964389984386 50-51 0.22794216237959508 52-53 0.23810656620487114 54-55 0.23205117669194072 56-57 0.19420499223612558 58-59 0.17971531018732778 60-61 0.190204109879368 62-63 0.19215048507995278 64-65 0.190204109879368 66-67 0.20177422912728862 68-69 0.19106916552407233 70-71 0.19463752005847776 72-73 0.21074918144109617 74-75 0.1987465343708234 76-77 0.20847841037374726 78-79 0.2059913753952223 80-81 0.192691144857893 82-83 0.20966786188521577 84-85 0.18977158205701583 86-87 0.19571883961435818 88-89 0.2039368682390495 90-91 0.19917906219317558 92-93 0.2059913753952223 94-95 0.20534258366169406 96-97 0.19507004788082993 98-99 0.20350434041669732 100-101 0.18641949143378647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 462398.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.032654298451273 #Duplication Level Percentage of deduplicated Percentage of total 1 76.18922624885649 22.88164693198396 2 10.354634920925099 6.219543419336296 3 3.824200967603585 3.4455271688352394 4 2.0352569532925813 2.444966739470211 5 1.22529854840396 1.8399483858305148 6 0.8357059812861747 1.5059081298669401 7 0.6605769560604267 1.3887215551220222 8 0.47616668254647726 1.1440439492286993 9 0.3969879409271159 1.0730341431466262 >10 3.2598075623624663 20.072730683039218 >50 0.4623975231240433 9.602348627781087 >100 0.2639189595435831 14.287437779677244 >500 0.007191252303639865 1.4273615546435123 >1k 0.005753001842911892 3.411715914465662 >5k 0.001438250460727973 3.3814797792485205 >10k+ 0.001438250460727973 5.873585238324249 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15500 3.352090623229339 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11696 2.5294227051155067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9358 2.0237976807858167 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6299 1.3622463764981683 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3100 0.6704181246458678 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2574 0.5566633073672463 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2339 0.505841288240866 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2134 0.46150718644976835 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1612 0.3486174248158513 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1578 0.34126445183586435 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1330 0.2876310018641949 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1130 0.24437821962897763 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 876 0.1894471861902517 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 822 0.177768934986743 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 800 0.17301112894086912 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 681 0.14727572351091484 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 671 0.14511308439915396 No Hit GTACATGGGAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG 635 0.13732758359681485 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 572 0.12370295719272142 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 547 0.11829635941331927 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 504 0.10899701123274755 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 501 0.1083482194992193 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 499 0.10791569167686711 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 481 0.10402294127569757 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 467 0.10099524651923233 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.081319555880432E-4 0.0 22-23 0.0 0.0 0.0 0.0017301112894086913 0.0 24-25 0.0 0.0 0.0 0.004433410179109771 0.0 26-27 0.0 0.0 0.0 0.007028577113222808 0.0 28-29 0.0 0.0 0.0 0.012759570759389098 0.0 30-31 0.0 0.0 0.0 0.02876310018641949 0.0 32-33 0.0 0.0 0.0 0.054498505616373774 0.0 34-35 0.0 0.0 0.0 0.08412666144749761 0.0 36-37 0.0 0.0 0.0 0.117647567679791 0.0 38-39 0.0 0.0 0.0 0.16165727360412457 0.0 40-41 0.0 0.0 0.0 0.22156237699990053 0.0 42-43 0.0 0.0 0.0 0.2788723134615634 0.0 44-45 0.0 0.0 0.0 0.35132072370555234 0.0 46-47 0.0 0.0 0.0 0.4442060735556815 0.0 48-49 0.0 0.0 0.0 0.5533112167440171 0.0 50-51 0.0 0.0 0.0 0.685664730383782 0.0 52-53 0.0 0.0 0.0 0.8342380373617533 0.0 54-55 0.0 0.0 0.0 0.9949221233655855 0.0 56-57 0.0 0.0 0.0 1.2041574574284493 0.0 58-59 0.0 0.0 0.0 1.4212864242492398 0.0 60-61 0.0 0.0 0.0 1.6802624578826033 0.0 62-63 0.0 0.0 0.0 1.9338318937365646 0.0 64-65 0.0 0.0 0.0 2.198538921016094 0.0 66-67 0.0 0.0 0.0 2.525529954714337 0.0 68-69 0.0 0.0 0.0 2.8890695894013385 0.0 70-71 0.0 0.0 0.0 3.3310048918896706 0.0 72-73 0.0 0.0 0.0 3.721685647429271 0.0 74-75 0.0 0.0 0.0 4.172595902231411 0.0 76-77 0.0 0.0 0.0 4.6014472380935905 0.0 78-79 0.0 0.0 0.0 5.091825656685366 0.0 80-81 0.0 0.0 0.0 5.599505188171229 0.0 82-83 0.0 0.0 0.0 6.151410689492602 0.0 84-85 0.0 0.0 0.0 6.7099122401048445 0.0 86-87 0.0 0.0 0.0 7.300853377393501 0.0 88-89 0.0 0.0 0.0 7.972569085506425 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACTA 25 0.0050050896 57.041058 5 AGATAGT 40 5.3829607E-4 47.503307 6 TCGACCG 25 0.0016159591 37.998528 18-19 GGTATCA 5715 0.0 37.69439 1 GTATGGT 55 0.002563482 34.585342 1 ATCCATC 55 0.0025771528 34.54786 7 GTATCAA 8825 0.0 33.409637 1 GGGATGC 100 1.2962537E-6 33.25231 7 GGTACTC 75 3.060701E-4 31.692913 3 TAGATAG 75 3.062649E-4 31.689474 5 TAGGCAT 75 3.062649E-4 31.689474 5 GTTCGAC 30 0.003949206 31.66887 16-17 CGACCGT 30 0.003951302 31.665438 18-19 GACTCCC 210 0.0 31.662004 9 GTACAGG 110 2.7043316E-6 30.262173 1 GTCACTA 95 3.4590026E-5 30.034637 1 GGGAACG 80 4.4876893E-4 29.689566 7 GCTTTGC 65 0.0058076466 29.26452 1 GTACATG 5990 0.0 28.580542 1 CATGGGT 320 0.0 28.226501 4 >>END_MODULE