##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937185_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 462398 Sequences flagged as poor quality 0 Sequence length 101 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67261969126164 31.0 31.0 34.0 30.0 34.0 2 31.83796642719043 31.0 31.0 34.0 30.0 34.0 3 31.81136380347666 31.0 31.0 34.0 30.0 34.0 4 35.63480162111428 37.0 35.0 37.0 33.0 37.0 5 35.5510836984589 37.0 35.0 37.0 33.0 37.0 6 35.68751811210256 37.0 35.0 37.0 33.0 37.0 7 35.61098663921557 37.0 35.0 37.0 33.0 37.0 8 35.63564937564609 37.0 35.0 37.0 33.0 37.0 9 37.16139126899338 39.0 37.0 39.0 33.0 39.0 10-11 37.092448496749554 39.0 37.0 39.0 33.0 39.0 12-13 36.9984731767871 39.0 37.0 39.0 33.0 39.0 14-15 37.77058291774618 39.5 37.0 41.0 32.0 41.0 16-17 37.82754575063041 39.0 37.0 41.0 32.5 41.0 18-19 38.06329720284258 40.0 37.0 41.0 33.0 41.0 20-21 38.100015571001606 40.0 37.0 41.0 33.0 41.0 22-23 37.93934662347155 40.0 36.5 41.0 33.0 41.0 24-25 37.761508484033236 40.0 36.0 41.0 33.0 41.0 26-27 37.50349049952638 39.0 36.0 41.0 32.5 41.0 28-29 37.276839432696505 39.0 35.5 41.0 32.0 41.0 30-31 36.86477125766115 38.5 35.0 40.0 32.0 41.0 32-33 36.35330386377104 38.0 35.0 40.0 31.0 41.0 34-35 36.01439020064966 38.0 35.0 40.0 31.0 41.0 36-37 35.641643129944335 38.0 35.0 40.0 30.0 41.0 38-39 35.33595301017738 37.0 35.0 40.0 30.0 41.0 40-41 34.972031669687155 37.0 35.0 40.0 28.0 41.0 42-43 34.44019978460115 37.0 34.0 40.0 25.0 41.0 44-45 33.703964982547504 37.0 33.0 40.0 21.0 41.0 46-47 32.798763186691986 36.0 33.0 40.0 11.0 41.0 48-49 32.086463393007755 36.0 33.0 40.0 2.0 41.0 50-51 31.717279270239057 36.0 32.0 40.0 2.0 41.0 52-53 31.46161099312713 35.0 31.5 40.0 2.0 41.0 54-55 31.189527203837386 35.0 31.0 40.0 2.0 41.0 56-57 30.89217513916583 35.0 31.0 39.5 2.0 41.0 58-59 30.623989506875027 35.0 31.0 39.0 2.0 41.0 60-61 30.272475226968975 35.0 29.5 39.0 2.0 40.5 62-63 29.870471974359752 35.0 28.5 39.0 2.0 40.0 64-65 29.497332384655643 35.0 28.0 38.0 2.0 40.0 66-67 29.054669354106203 35.0 27.5 38.0 2.0 40.0 68-69 28.623666192327825 35.0 26.0 37.5 2.0 40.0 70-71 28.195676668151677 34.0 26.0 37.0 2.0 39.0 72-73 27.70730734129473 34.0 25.5 36.0 2.0 39.0 74-75 27.218626594405684 34.0 24.5 36.0 2.0 39.0 76-77 25.95028308945973 32.0 22.5 35.0 2.0 37.0 78-79 26.494742624319308 33.0 23.5 35.0 2.0 37.0 80-81 26.242829770024954 33.0 21.0 35.0 2.0 37.0 82-83 25.799104883671642 33.0 19.0 35.0 2.0 36.5 84-85 25.27587057037444 33.0 14.5 35.0 2.0 36.0 86-87 24.779431355671953 33.0 4.5 35.0 2.0 36.0 88-89 24.208946621741443 32.0 2.0 35.0 2.0 35.0 90-91 23.58273500317908 31.5 2.0 34.5 2.0 35.0 92-93 23.02387769843295 31.0 2.0 34.0 2.0 35.0 94-95 22.409722792918657 31.0 2.0 34.0 2.0 35.0 96-97 21.75336290381879 30.5 2.0 34.0 2.0 35.0 98-99 21.196363306069664 30.0 2.0 34.0 2.0 35.0 100-101 19.56647628233686 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.009371064585828037 1101 2 0.03642558760267178 1101 3 -0.04380763778254604 1101 4 0.1154647003421232 1101 5 -0.02626941851397646 1101 6 0.04122287260450008 1101 7 -0.21454924483257543 1101 8 0.1572959169009991 1101 9 0.3237215095833861 1101 10-11 -0.12398601054718483 1101 12-13 -0.04097612567818487 1101 14-15 -0.0809918259565805 1101 16-17 -0.17396143859984647 1101 18-19 -0.08367524705997909 1101 20-21 0.011615236017881614 1101 22-23 0.053003852056988876 1101 24-25 0.1294274040025627 1101 26-27 0.06305218822729586 1101 28-29 0.07291707723294394 1101 30-31 0.35498556480251153 1101 32-33 0.36162702901156507 1101 34-35 0.2657253770315364 1101 36-37 0.2944011502525399 1101 38-39 0.2925312849740038 1101 40-41 -0.04992532234827962 1101 42-43 0.5075590995338715 1101 44-45 0.3908977449350175 1101 46-47 0.47543756052444053 1101 48-49 0.8573288260422736 1101 50-51 0.5888802237693547 1101 52-53 0.3187792490374939 1101 54-55 0.027380761766440997 1101 56-57 0.31182410750270506 1101 58-59 -0.1298965602595885 1101 60-61 0.01945381598136109 1101 62-63 0.14178769632828292 1101 64-65 0.31478370674278366 1101 66-67 0.37001560756720053 1101 68-69 0.19089671201719227 1101 70-71 0.31986669322598615 1101 72-73 0.802782025514734 1101 74-75 0.7434917673323831 1101 76-77 0.5072247042828266 1101 78-79 0.7345817883885601 1101 80-81 0.8896296501622736 1101 82-83 0.9698466385016786 1101 84-85 0.8250926208024829 1101 86-87 0.8183590145193662 1101 88-89 0.9371442030085007 1101 90-91 1.3433847636213443 1101 92-93 1.0342353080542637 1101 94-95 1.0050633103905682 1101 96-97 1.2351847256447783 1101 98-99 1.331475891080327 1101 100-101 1.5132543107209635 1102 1 -0.08375216837869814 1102 2 -0.020531316780701303 1102 3 0.046684640209733175 1102 4 -0.0408278842592793 1102 5 0.011964456116956512 1102 6 0.029143040671726794 1102 7 -0.09568852486891188 1102 8 0.11156005498866506 1102 9 -0.03031976909024081 1102 10-11 -0.07020875640228041 1102 12-13 0.11289978279330626 1102 14-15 0.047290737068635735 1102 16-17 -0.0681919348524005 1102 18-19 -0.0024628634578931496 1102 20-21 -0.18803202153378606 1102 22-23 -0.12051775253079455 1102 24-25 -0.005335115870252594 1102 26-27 -0.20584472864756265 1102 28-29 -0.024065803757295612 1102 30-31 0.014095830530415299 1102 32-33 0.027405333576631108 1102 34-35 0.2169234238178248 1102 36-37 0.3089012070320081 1102 38-39 0.07602407398150035 1102 40-41 0.13245033061561173 1102 42-43 -0.009517325630788775 1102 44-45 0.1456672202927365 1102 46-47 0.05697997478744554 1102 48-49 0.2242803363761432 1102 50-51 0.33889441863648884 1102 52-53 0.3836426944156486 1102 54-55 0.23421417080573192 1102 56-57 0.38300426875639815 1102 58-59 0.6070406132584658 1102 60-61 0.6887347040122513 1102 62-63 0.4980291794080003 1102 64-65 0.570099684485232 1102 66-67 0.5577995049099229 1102 68-69 0.6128497837864408 1102 70-71 0.4255119918859904 1102 72-73 0.1626644920148479 1102 74-75 0.2751214800282682 1102 76-77 0.22473975084199083 1102 78-79 0.0943755369690713 1102 80-81 0.07765486024630164 1102 82-83 0.4704428229213917 1102 84-85 0.4619843707457001 1102 86-87 0.44927472539831115 1102 88-89 0.6178235693496319 1102 90-91 0.8879885848795759 1102 92-93 0.7317568842523094 1102 94-95 0.4307815706676479 1102 96-97 0.39346459164523395 1102 98-99 0.8493578749549577 1102 100-101 1.0716165069508072 1103 1 -0.03268558790035314 1103 2 0.08097563215271819 1103 3 0.3074447134698062 1103 4 0.22995831483574136 1103 5 -0.1823430745876351 1103 6 -0.017385186003558317 1103 7 0.14600911572578923 1103 8 -0.06886980926472575 1103 9 -0.023471837609960744 1103 10-11 -0.06931320587437995 1103 12-13 -0.044861879563939056 1103 14-15 -0.082823327788077 1103 16-17 0.09217557753716932 1103 18-19 -0.05937072275546029 1103 20-21 -0.14455742015477568 1103 22-23 -0.04829799924485911 1103 24-25 0.10831563289078616 1103 26-27 0.20796358313869234 1103 28-29 0.09501884933471416 1103 30-31 -0.08883187901493983 1103 32-33 0.17179433917888076 1103 34-35 0.32430868561484516 1103 36-37 0.3056624115138007 1103 38-39 0.464915207357933 1103 40-41 0.6276561052331431 1103 42-43 0.4515497935245705 1103 44-45 0.39911475315203404 1103 46-47 0.5771601622470399 1103 48-49 0.9590761777896297 1103 50-51 0.9307523156414454 1103 52-53 0.9506473809056288 1103 54-55 0.7981955325812145 1103 56-57 0.5895936352722337 1103 58-59 0.6718804915174665 1103 60-61 0.47525027177782064 1103 62-63 0.7090087635493489 1103 64-65 0.5340689260280023 1103 66-67 0.7486662362178294 1103 68-69 1.1064496275701075 1103 70-71 1.0632584366177298 1103 72-73 1.02676974950246 1103 74-75 0.8530848769254895 1103 76-77 0.7158179128760338 1103 78-79 0.572097875904646 1103 80-81 0.7577615182941386 1103 82-83 0.8989873176423586 1103 84-85 1.1773844730943317 1103 86-87 1.341401287561638 1103 88-89 0.9739227397870387 1103 90-91 1.1200405402771203 1103 92-93 1.1239541477731017 1103 94-95 1.0580036133308681 1103 96-97 1.091758332218383 1103 98-99 0.9959150555194896 1103 100-101 1.15103769850435 1104 1 0.13621772085670258 1104 2 0.008050385552881067 1104 3 0.036744055200593095 1104 4 -0.09151545819196372 1104 5 -0.016623967369440606 1104 6 -0.11730415795177862 1104 7 0.04224633371803321 1104 8 -0.1686448057490466 1104 9 0.016496420822647906 1104 10-11 0.06504436221262466 1104 12-13 0.14268625176846683 1104 14-15 0.054030373050999 1104 16-17 -0.03648993732578276 1104 18-19 -0.04927737308258173 1104 20-21 -0.05118430941420371 1104 22-23 -0.08277007804966985 1104 24-25 0.00806518254892552 1104 26-27 -0.003543069867049553 1104 28-29 -0.07199938532519923 1104 30-31 -0.21999520076326462 1104 32-33 -0.32084951909569526 1104 34-35 -0.3014522079705486 1104 36-37 -0.6411034977749566 1104 38-39 -0.40020656909840113 1104 40-41 -0.1576346247559428 1104 42-43 -0.013422315414636898 1104 44-45 0.2737723928919422 1104 46-47 0.05108888434760672 1104 48-49 -0.11749700527350271 1104 50-51 -0.088470082922818 1104 52-53 -0.03340147700063767 1104 54-55 0.09646414392112135 1104 56-57 0.2343693348925413 1104 58-59 0.43218478198628674 1104 60-61 0.5400258374674642 1104 62-63 0.22662566827777297 1104 64-65 0.19256917045146693 1104 66-67 0.15984676805649656 1104 68-69 0.1220056742047646 1104 70-71 0.17014515136550656 1104 72-73 0.4719921078875231 1104 74-75 0.4823463832436694 1104 76-77 0.5322224102677353 1104 78-79 0.6675100810060641 1104 80-81 0.7517999296086018 1104 82-83 0.7455396075218843 1104 84-85 1.0546035367850592 1104 86-87 1.18995416673609 1104 88-89 1.2547435338619444 1104 90-91 1.4273863023760818 1104 92-93 1.201061379359313 1104 94-95 1.022088431620439 1104 96-97 1.2159555853004633 1104 98-99 0.9449343482316515 1104 100-101 1.0570752928417626 1105 1 0.15229609708133296 1105 2 0.1948105152183679 1105 3 0.2282674804464193 1105 4 0.15698001108820847 1105 5 0.04983500374429184 1105 6 0.12378133977835404 1105 7 0.10966084860829284 1105 8 -0.03829781715427316 1105 9 0.18433317788736758 1105 10-11 0.12052900319860527 1105 12-13 0.21063262593056464 1105 14-15 0.26234432507188643 1105 16-17 0.42022573251040285 1105 18-19 0.4705672879517877 1105 20-21 0.17412389852654542 1105 22-23 0.041234263364323454 1105 24-25 0.12634888169326786 1105 26-27 0.1311813909718822 1105 28-29 0.11079794203688209 1105 30-31 0.1549691917092062 1105 32-33 0.3307123442507276 1105 34-35 0.428563405254188 1105 36-37 0.36751081951605613 1105 38-39 0.6890628545825024 1105 40-41 0.8596228564306614 1105 42-43 0.5753170519072128 1105 44-45 0.5518338289480269 1105 46-47 0.674159316938507 1105 48-49 0.910823148767367 1105 50-51 1.1143251530604381 1105 52-53 0.8573816722553005 1105 54-55 0.893926724466251 1105 56-57 0.7355502427673031 1105 58-59 0.7457041038026127 1105 60-61 0.8542302661424301 1105 62-63 0.8000907584774986 1105 64-65 0.9289688978510533 1105 66-67 1.1111627140989242 1105 68-69 0.9903605884041475 1105 70-71 0.9888712853074999 1105 72-73 0.9302636914579416 1105 74-75 0.8648487540739822 1105 76-77 0.9498274930394608 1105 78-79 1.1590237705228503 1105 80-81 0.9839519944846167 1105 82-83 1.054805858076282 1105 84-85 0.972819806991204 1105 86-87 1.083652625966824 1105 88-89 0.846210708228849 1105 90-91 0.9216043610717115 1105 92-93 1.0126551903202987 1105 94-95 1.0021428074700651 1105 96-97 1.3826853539146384 1105 98-99 1.2573971631554421 1105 100-101 1.5169154105359084 1106 1 -0.06687848663508333 1106 2 -0.03821792329581797 1106 3 -0.048683885686681805 1106 4 0.023246699757592637 1106 5 -0.10968863739136481 1106 6 -0.2905927221593032 1106 7 -0.1620334393287166 1106 8 -0.2135219698531472 1106 9 -0.35605359847857443 1106 10-11 -0.30679915949578884 1106 12-13 -0.5207750765926704 1106 14-15 -0.4320162994910106 1106 16-17 -0.5777016899496559 1106 18-19 -0.1887068580676896 1106 20-21 -0.1630913430711658 1106 22-23 0.05165019333281151 1106 24-25 -0.031469548727066865 1106 26-27 -0.024024216578197866 1106 28-29 0.10457038677469654 1106 30-31 -0.15688202276228935 1106 32-33 0.13385087494058467 1106 34-35 -0.042597316749322545 1106 36-37 0.27729967199568506 1106 38-39 0.31501663315656714 1106 40-41 0.3375710243114156 1106 42-43 0.46339982927898404 1106 44-45 0.31791938809212184 1106 46-47 0.48051378074010387 1106 48-49 0.5337044048082937 1106 50-51 0.6800445240970028 1106 52-53 0.4488304727142136 1106 54-55 0.8286159753522533 1106 56-57 1.0727379809663837 1106 58-59 0.8103194098368611 1106 60-61 0.9682416761238066 1106 62-63 0.6343002715101811 1106 64-65 0.601232506139791 1106 66-67 0.40744504041495944 1106 68-69 0.473176857843157 1106 70-71 0.6687376187901393 1106 72-73 0.6171846466902657 1106 74-75 0.6637105062762174 1106 76-77 0.17951623314805687 1106 78-79 0.6699445843091212 1106 80-81 0.411115136787199 1106 82-83 0.08559984190428338 1106 84-85 -0.04056059644436161 1106 86-87 -0.059934760188749436 1106 88-89 0.10879351310402541 1106 90-91 -0.31959651589379945 1106 92-93 -0.26811129010906853 1106 94-95 -0.26688365079942145 1106 96-97 0.04757608684091963 1106 98-99 0.35448808939132803 1106 100-101 0.5573866616262215 1107 1 0.10934737913261472 1107 2 0.03317343435051967 1107 3 -0.08312305209796023 1107 4 -0.03542630054887752 1107 5 -0.1301452723898322 1107 6 -0.17498591860429258 1107 7 -0.13723264251596845 1107 8 -0.05733134772626869 1107 9 -0.06852678266490386 1107 10-11 -0.0016850007461712835 1107 12-13 -0.05699557919763265 1107 14-15 -0.36519512265987686 1107 16-17 -0.2590148986533052 1107 18-19 -0.13253922092395243 1107 20-21 -0.04735046044780944 1107 22-23 0.16374412529726357 1107 24-25 -0.08829608872093075 1107 26-27 -0.259252534077433 1107 28-29 -0.11107089425502892 1107 30-31 -0.21497656765962603 1107 32-33 -0.28516536778082724 1107 34-35 0.19271527902144214 1107 36-37 -0.03394381559242987 1107 38-39 -0.13842728348456035 1107 40-41 0.03997295504999698 1107 42-43 -0.29011687314210377 1107 44-45 -0.28431015527287684 1107 46-47 -0.235751925665042 1107 48-49 -0.1514549577415103 1107 50-51 0.028600300989428007 1107 52-53 -0.49330866305042065 1107 54-55 -0.36233560294992273 1107 56-57 0.19632440450300592 1107 58-59 0.1179427625797338 1107 60-61 0.2747923963199419 1107 62-63 0.45603257307315914 1107 64-65 0.25774108720016287 1107 66-67 0.24710945966105058 1107 68-69 0.22041482903531318 1107 70-71 0.32548371134300425 1107 72-73 0.35763055536326505 1107 74-75 0.29566729450790774 1107 76-77 0.2361933707514936 1107 78-79 -0.16819516438839344 1107 80-81 -0.11091980038717963 1107 82-83 -0.002294733639693902 1107 84-85 -0.023210765000907685 1107 86-87 0.1805954267557759 1107 88-89 0.07808940645370299 1107 90-91 0.18336471610129834 1107 92-93 0.022763671582627154 1107 94-95 0.1200915254187791 1107 96-97 -0.40022884726879226 1107 98-99 -0.054954907850476076 1107 100-101 0.4459094933761456 1108 1 -0.3544077588591641 1108 2 -0.3644777694075536 1108 3 -0.24100619948492508 1108 4 -0.24214972979138594 1108 5 -0.264907456770338 1108 6 -0.2570323073300713 1108 7 -0.15040058144726487 1108 8 -0.21449517626414405 1108 9 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-0.8414320057534077 2316 58-59 -0.43833928870231986 2316 60-61 -0.7841819626544151 2316 62-63 -1.2262110166704296 2316 64-65 -1.3729640410049662 2316 66-67 -1.078916181364587 2316 68-69 -1.3610595299390482 2316 70-71 -1.5197085963493002 2316 72-73 -1.5666002138675061 2316 74-75 -1.6042045003638883 2316 76-77 -1.054255347197227 2316 78-79 -1.3524259336191662 2316 80-81 -1.0248941593615406 2316 82-83 -0.8835767849217433 2316 84-85 -1.192121021411161 2316 86-87 -0.7801738040134545 2316 88-89 -0.73376956790527 2316 90-91 -1.1601609249243445 2316 92-93 -1.0033901745712193 2316 94-95 -1.169267448940193 2316 96-97 -1.2790108985508475 2316 98-99 -1.1538651642607327 2316 100-101 -0.8647315322648801 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 13.0 5 49.0 6 94.0 7 228.0 8 377.0 9 742.0 10 1264.0 11 2105.0 12 3150.0 13 4262.0 14 5297.0 15 7842.0 16 10860.0 17 14031.0 18 11093.0 19 6055.0 20 4903.0 21 4863.0 22 5174.0 23 5620.0 24 6318.0 25 7048.0 26 7863.0 27 9101.0 28 10671.0 29 12680.0 30 14747.0 31 17088.0 32 20838.0 33 24853.0 34 33435.0 35 49523.0 36 49048.0 37 64031.0 38 44141.0 39 2986.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.67036433620449 23.538944530629642 17.63327513127917 15.157416001886698 2 15.226276930263538 27.06997002582191 39.15393232669692 18.549820717217635 3 17.664003737040385 33.54599284598982 33.027391986989564 15.762611429980232 4 10.512372458358383 22.366229957742032 38.924476316939085 28.196921266960494 5 8.730957000620796 43.732654999145595 33.295479863035325 14.24090813719828 6 22.11558008468895 43.33604384102007 17.627455136051626 16.92092093823935 7 20.924830989753413 41.395075238214694 22.149317254832418 15.530776517199469 8 25.808502631931802 34.29750993732672 20.326212483618008 19.567774947123475 9 27.576892633618655 17.432385088170797 22.345468622269127 32.64525365594142 10-11 22.494366325113862 31.913740976388304 25.680041868693205 19.911850829804628 12-13 25.86185828985429 31.595144764942823 27.835419426346945 14.707577518855938 14-15 22.21538587969671 30.937958209161803 30.962287899169116 15.884368011972368 16-17 15.139014442103987 34.2976180692823 31.671525395871093 18.891842092742618 18-19 14.55045220783827 29.560140831058956 40.46546481602429 15.423942145078481 20-21 16.529483259010636 28.05429521753987 41.443842750184906 13.972378773264591 22-23 16.10500944535696 28.690528583153203 41.331843991033665 13.872617980456168 24-25 15.568947097522049 29.517861236424032 41.34382069126596 13.569370974787953 26-27 14.531283284547536 29.645292828325537 42.210185869023384 13.61323801810355 28-29 14.217805508396683 29.932848167652498 42.17751440898818 13.67183191496264 30-31 14.56807771659912 30.173573415109928 41.34457761495508 13.913771253335872 32-33 13.886413868572095 31.130973749886458 41.26769579453198 13.71491658700946 34-35 14.337432255329825 30.681036682684613 41.17232340970333 13.809207652282232 36-37 14.502549751512767 30.466719146709114 40.62539197833901 14.405339123439115 38-39 13.7579531053335 31.6117284244309 40.62668956180606 14.003628908429535 40-41 13.84586438522658 31.422064974329473 40.669185420352164 14.062885220091784 42-43 13.924243653175438 31.5635223186525 39.29121042528456 15.2210236028875 44-45 14.317211579634858 31.526952971249877 38.68150381273276 15.47433163638251 46-47 14.928157128707303 32.21088759034425 37.58807347782646 15.272881803121985 48-49 14.73114070562589 32.69023654946604 36.4138685720959 16.164754172812167 50-51 14.692753861392132 32.83145688346403 35.73966582900445 16.736123426139386 52-53 15.096951111380239 33.562212639328024 34.68948827633337 16.651347972958362 54-55 15.420806318366429 33.11865535750587 34.24874242535651 17.211795898771186 56-57 16.03077867983858 33.21132444344482 33.55377834679216 17.204118529924436 58-59 15.901777256822044 32.64860574656465 34.249174953178866 17.200442043434442 60-61 16.277643934446083 33.52447458682779 33.954623506157034 16.243257972569086 62-63 16.557056907685585 33.30680496022907 34.48976855436226 15.646369577723087 64-65 16.73915112089585 33.29728934813732 34.35384668618809 15.60971284477874 66-67 16.70173746426239 33.49754972988637 33.55842802088244 16.242284784968795 68-69 16.30813714592191 33.428885938087966 34.10016911837854 16.16280779761158 70-71 16.798948092336037 33.54404647078923 33.92640106574855 15.730604371126173 72-73 16.194922988421233 33.30767001587377 34.133041232877304 16.364365762827692 74-75 15.263582454941412 34.32508358600167 33.47084113685613 16.94049282220079 76-77 14.726815427402368 33.50706534197812 34.88780228288185 16.878316947737662 78-79 14.422640236333203 33.41601823537299 34.66440166263695 17.496939865656856 80-81 14.758498090389665 33.82886604180813 33.82151306882815 17.591122798974045 82-83 14.821014386972248 33.24758459983023 33.76196886877632 18.169432144421197 84-85 14.39228708881072 33.226570010175315 33.475059662387125 18.90608323862684 86-87 14.290286722693438 33.481438068503756 33.38411930847452 18.84415590032829 88-89 14.554993749972967 32.77944541282618 33.31869947534375 19.3468613618571 90-91 14.618114808878968 32.284130455000636 33.56302895419281 19.534725781927584 92-93 14.97771919371704 32.76203060812922 33.105028509267534 19.155221688886197 94-95 14.689834298591256 31.95623683493441 33.34930081877517 20.004628047699168 96-97 14.704323980640055 31.9246623039027 33.19748355312955 20.17353016232769 98-99 14.669181095073942 32.076263305637134 32.95883632714675 20.29571927214218 100-101 14.437286482265929 31.7341289882328 33.5535515184024 20.275033011098866 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6756.0 1 4365.0 2 2363.5 3 2781.5 4 2325.0 5 2242.0 6 3870.5 7 7444.5 8 10263.5 9 10299.0 10 7873.5 11 5334.0 12 4695.5 13 4422.0 14 3959.0 15 3528.0 16 3219.5 17 3000.0 18 2826.5 19 2680.5 20 2748.5 21 2847.0 22 2934.5 23 3135.0 24 3322.5 25 3789.5 26 4714.5 27 5698.0 28 5983.0 29 6392.0 30 7721.0 31 8962.0 32 10939.0 33 13828.5 34 15972.0 35 17795.5 36 20653.0 37 22875.5 38 22326.0 39 20517.5 40 18389.5 41 16088.0 42 13888.0 43 13513.5 44 13614.0 45 12443.5 46 10697.0 47 10246.5 48 12251.5 49 10872.5 50 7065.5 51 4645.5 52 3077.5 53 2503.5 54 2241.5 55 2076.5 56 2019.5 57 1951.5 58 1831.5 59 1650.0 60 1481.5 61 1468.5 62 1236.5 63 711.0 64 387.5 65 342.0 66 341.5 67 257.0 68 180.5 69 143.0 70 105.0 71 93.5 72 94.5 73 112.5 74 106.0 75 68.0 76 52.0 77 43.0 78 30.5 79 22.0 80 16.0 81 9.5 82 3.0 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04649674090285858 2 0.0 3 0.0 4 0.0 5 0.01924748809467169 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0022707710673489073 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.243958667641296E-4 24-25 0.0 26-27 5.40659777940216E-4 28-29 0.002811430845289123 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.013083966626153227 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.081319555880432E-4 84-85 9.731876002923889E-4 86-87 0.0 88-89 0.0 90-91 0.010164403825276062 92-93 9.731876002923889E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.011570119247920622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 462398.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.727573151371402 #Duplication Level Percentage of deduplicated Percentage of total 1 76.66409344129234 22.790374458595902 2 10.073067483012142 5.98895700919888 3 3.7744439553272056 3.366151763632233 4 1.8688096349541783 2.2222070051635203 5 1.2130270114135395 1.803017460819271 6 0.8591683901925656 1.53245947012773 7 0.6169553362364726 1.2838409422369073 8 0.5205241337432799 1.2379135410326068 9 0.41331235477531936 1.1058095934853989 >10 3.2516566579665924 19.912816501381112 >50 0.4592105760121997 9.464409714662139 >100 0.2696951001976411 14.150460460787956 >500 0.00801796243830825 1.536953571731731 >1k 0.005102339733468886 3.7200647003370024 >5k 7.289056762098409E-4 1.837497009486124 >10k+ 0.0021867170286295227 8.047066797321484 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14439 3.1226346134715115 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12472 2.6972435001881494 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10226 2.2115147556866597 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8480 1.8339179667732126 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3774 0.81618000077855 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3254 0.7037227669669852 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2448 0.5294140545590595 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2252 0.48702632796854656 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1978 0.4277700163062989 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1963 0.42452605763865764 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1499 0.3241796028529535 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 922 0.19939532610435168 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 864 0.18685201925613865 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 737 0.1593865025367757 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 656 0.14186912573151267 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 625 0.135164944485054 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 583 0.12608186021565837 No Hit GTACATGGGAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG 578 0.12500054065977792 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 568 0.12283790154801706 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 534 0.11548492856803014 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 518 0.11202470598921276 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 508 0.10986206687745188 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 488 0.10553678865393015 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.162639111760864E-4 0.0 22-23 0.0 0.0 0.0 0.0019463752005847777 0.0 24-25 0.0 0.0 0.0 0.004649674090285858 0.0 26-27 0.0 0.0 0.0 0.00746110493557498 0.0 28-29 0.0 0.0 0.0 0.01319209858174127 0.0 30-31 0.0 0.0 0.0 0.02908749605318362 0.0 32-33 0.0 0.0 0.0 0.05536356126107812 0.0 34-35 0.0 0.0 0.0 0.08553237687014217 0.0 36-37 0.0 0.0 0.0 0.11959394288037578 0.0 38-39 0.0 0.0 0.0 0.16360364880470937 0.0 40-41 0.0 0.0 0.0 0.22221116873342878 0.0 42-43 0.0 0.0 0.0 0.27941297323950365 0.0 44-45 0.0 0.0 0.0 0.35305083499496104 0.0 46-47 0.0 0.0 0.0 0.4459361848450901 0.0 48-49 0.0 0.0 0.0 0.5545006682554855 0.0 50-51 0.0 0.0 0.0 0.6852322025614297 0.0 52-53 0.0 0.0 0.0 0.8341299054061653 0.0 54-55 0.0 0.0 0.0 0.9941651996764692 0.0 56-57 0.0 0.0 0.0 1.2004809709384556 0.0 58-59 0.0 0.0 0.0 1.4179343336260104 0.0 60-61 0.0 0.0 0.0 1.6785323465931947 0.0 62-63 0.0 0.0 0.0 1.932642442225096 0.0 64-65 0.0 0.0 0.0 2.1972413375490376 0.0 66-67 0.0 0.0 0.0 2.526611274270217 0.0 68-69 0.0 0.0 0.0 2.891015964601923 0.0 70-71 0.0 0.0 0.0 3.3300317042893783 0.0 72-73 0.0 0.0 0.0 3.7185498207172176 0.0 74-75 0.0 0.0 0.0 4.165134797295837 0.0 76-77 0.0 0.0 0.0 4.592256021868607 0.0 78-79 0.0 0.0 0.0 5.084256419794203 0.0 80-81 0.0 0.0 0.0 5.594855514080944 0.0 82-83 0.0 0.0 0.0 6.148815522558488 0.0 84-85 0.0 0.0 0.0 6.705370697970147 0.0 86-87 0.0 0.0 0.0 7.294149196147043 0.0 88-89 0.0 0.0 0.0 7.96067457039174 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 20 0.0020737303 71.24619 8 AGATAGT 60 8.285673E-5 39.581215 6 GTATGGT 95 8.637271E-7 35.024643 1 TAGATAG 55 0.00257598 34.55108 5 GATAGTC 55 0.0025787146 34.543606 7 CATGGAT 70 2.0494798E-4 33.934093 4 GTACCAC 85 1.6282864E-5 33.53487 3 GGTATCA 7025 0.0 33.357986 1 AAGATAG 60 0.0039453846 31.671824 5 TACCTGG 90 2.4071856E-5 31.671822 2 GTATCAA 10295 0.0 30.380772 1 AGTACCA 95 3.4825054E-5 30.004885 2 GCATCGA 65 0.0058420342 29.229204 8 GACAATG 180 2.1100277E-10 29.026224 7 GGACAAT 180 2.1100277E-10 29.026224 6 ACCATAC 100 4.948597E-5 28.498476 8 GAGTACC 135 4.190024E-7 28.167963 1 GGTGGCG 70 0.008388665 27.141403 9 GTAGATA 125 7.3814717E-6 26.598577 6 ATGGGAG 935 0.0 26.421413 5 >>END_MODULE