##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937184_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 484507 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08144154780013 33.0 31.0 34.0 31.0 34.0 2 32.09881797373413 33.0 31.0 34.0 30.0 34.0 3 32.0614191332633 33.0 31.0 34.0 30.0 34.0 4 35.770525503243505 37.0 35.0 37.0 35.0 37.0 5 35.83614271826826 37.0 35.0 37.0 35.0 37.0 6 35.95362915293278 37.0 36.0 37.0 35.0 37.0 7 35.87105862247604 37.0 35.0 37.0 35.0 37.0 8 35.89855667719971 37.0 36.0 37.0 35.0 37.0 9 37.523653941016335 39.0 37.0 39.0 35.0 39.0 10-11 37.456731275296335 39.0 37.0 39.0 35.0 39.0 12-13 37.3260757842508 39.0 37.0 39.0 35.0 39.0 14-15 38.32551026094566 40.0 38.0 41.0 34.0 41.0 16-17 38.35962328717645 40.0 38.0 41.0 34.5 41.0 18-19 38.55209625454327 40.0 38.0 41.0 35.0 41.0 20-21 38.59114213004146 40.0 38.0 41.0 35.0 41.0 22-23 38.4680149100013 40.0 38.0 41.0 35.0 41.0 24-25 38.35665944970867 40.0 38.0 41.0 34.5 41.0 26-27 38.25511499317864 40.0 38.0 41.0 34.5 41.0 28-29 38.08133835011672 40.0 37.0 41.0 34.0 41.0 30-31 37.61213150687193 39.0 36.5 41.0 34.0 41.0 32-33 37.05958634240578 38.5 35.0 41.0 33.5 41.0 34-35 36.80472727948203 39.0 35.0 41.0 33.0 41.0 36-37 36.53872596267959 38.5 35.0 41.0 33.0 41.0 38-39 36.29786876144205 38.0 35.0 41.0 33.0 41.0 40-41 35.93171718881254 38.0 35.0 40.5 31.5 41.0 42-43 35.41070614046856 38.0 35.0 40.0 30.0 41.0 44-45 34.795790360097996 38.0 35.0 40.0 26.0 41.0 46-47 33.974503980334646 38.0 35.0 40.0 19.0 41.0 48-49 33.29047361544828 38.0 34.0 40.0 4.5 41.0 50-51 32.15608752814717 36.5 32.0 39.5 2.0 40.5 52-53 32.26435221782141 37.0 33.0 39.5 2.0 40.5 54-55 32.57259234644701 37.0 33.0 40.0 2.0 41.0 56-57 32.33472581407493 37.0 33.0 40.0 2.0 41.0 58-59 32.08731865587082 36.0 33.0 40.0 2.0 41.0 60-61 31.80006377616835 36.0 33.0 40.0 2.0 41.0 62-63 31.493259127319106 35.5 33.0 40.0 2.0 41.0 64-65 31.100014034884943 35.0 32.0 39.0 2.0 41.0 66-67 30.7371214450978 35.0 31.5 39.0 2.0 41.0 68-69 30.265928046447215 35.0 31.0 39.0 2.0 40.0 70-71 29.80375515730423 35.0 30.5 38.0 2.0 40.0 72-73 29.334335726831604 35.0 29.5 37.5 2.0 40.0 74-75 28.8416864978215 35.0 29.0 37.0 2.0 39.0 76-77 28.355823548472983 35.0 28.5 36.0 2.0 39.0 78-79 27.83357515990481 34.5 26.5 36.0 2.0 38.5 80-81 27.289089734513638 34.0 26.0 35.5 2.0 37.5 82-83 26.753549484321177 34.0 24.5 35.0 2.0 37.0 84-85 26.14051499771933 34.0 21.0 35.0 2.0 36.5 86-87 25.647442658207208 33.0 16.5 35.0 2.0 36.0 88-89 25.109215140338527 33.0 5.0 35.0 2.0 36.0 90-91 24.543470992163165 33.0 2.0 35.0 2.0 35.0 92-93 24.02048164422805 33.0 2.0 35.0 2.0 35.0 94-95 23.512819216234234 32.0 2.0 35.0 2.0 35.0 96-97 22.82042055119947 32.0 2.0 35.0 2.0 35.0 98-99 22.05532015017327 31.0 2.0 34.5 2.0 35.0 100-101 19.989553298507555 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10925345931394759 1101 2 0.13760820268558405 1101 3 0.08321409124077661 1101 4 0.09692081918711892 1101 5 -0.020626656474313165 1101 6 -0.18885414894344166 1101 7 -0.12587227509345666 1101 8 -0.012515295427618867 1101 9 -0.1034345621677275 1101 10-11 -0.20664659367891147 1101 12-13 -0.10374174166577177 1101 14-15 -0.17991630601655828 1101 16-17 -0.19380640983332142 1101 18-19 0.07889425122366589 1101 20-21 0.04049717861618518 1101 22-23 -0.08211064529948686 1101 24-25 -0.07357651859786074 1101 26-27 -0.10745192874946241 1101 28-29 -0.23736772416742724 1101 30-31 -0.10959990509934414 1101 32-33 -0.2121432979004041 1101 34-35 -0.2587893817689917 1101 36-37 -0.23850780940542649 1101 38-39 -0.48535332933388275 1101 40-41 -0.4529523298036082 1101 42-43 -0.33423239205756516 1101 44-45 -0.05741486891719916 1101 46-47 0.08296758182019914 1101 48-49 0.19592307996641267 1101 50-51 0.23281864821679932 1101 52-53 0.3243001456955241 1101 54-55 0.223255252611132 1101 56-57 -0.0180549652566242 1101 58-59 -0.07794400314617178 1101 60-61 0.0904706567856195 1101 62-63 -0.07881358939875582 1101 64-65 0.14043865421079005 1101 66-67 0.16913637967174466 1101 68-69 0.020814971609155464 1101 70-71 -0.0744092306633668 1101 72-73 0.1999960346534202 1101 74-75 -0.11109266909678084 1101 76-77 -0.09508249268266056 1101 78-79 -0.09493109991879933 1101 80-81 0.038912040463049635 1101 82-83 -0.028017950510299983 1101 84-85 0.1748579692861334 1101 86-87 0.16697138350532725 1101 88-89 0.21377125579975242 1101 90-91 0.580024362191363 1101 92-93 0.567297621249125 1101 94-95 0.5758840890582775 1101 96-97 0.7431024599824774 1101 98-99 0.801263809180842 1101 100-101 0.8301738308290183 1102 1 0.027882207109357182 1102 2 -0.03071190313451666 1102 3 -0.05216950664282649 1102 4 0.0883008897338513 1102 5 -0.2011822120298703 1102 6 -0.10547672389935059 1102 7 -0.08217738973190336 1102 8 -0.0278592107715383 1102 9 -0.08039664329647422 1102 10-11 -0.0478172285995484 1102 12-13 -0.20936343478746267 1102 14-15 0.02970670985646251 1102 16-17 -0.016017609127857213 1102 18-19 -0.06448096923488578 1102 20-21 0.04521499166732923 1102 22-23 -0.09469882872100044 1102 24-25 0.10699005988539056 1102 26-27 -0.043540994005198286 1102 28-29 -0.07280643668946851 1102 30-31 -0.11558535483479915 1102 32-33 -0.07000264534308087 1102 34-35 -0.18574043997005418 1102 36-37 -0.13884952016379515 1102 38-39 0.13270222622167438 1102 40-41 0.1713642486796303 1102 42-43 0.11171117055265967 1102 44-45 -0.12382800824700269 1102 46-47 0.06247595924524063 1102 48-49 0.10980094592760992 1102 50-51 0.4148182072996889 1102 52-53 0.45283850548388926 1102 54-55 0.5567539298598163 1102 56-57 0.4333890382707182 1102 58-59 0.48138139367922506 1102 60-61 0.5129243604893219 1102 62-63 0.7062503435818464 1102 64-65 0.5518143155864479 1102 66-67 0.36570824916028144 1102 68-69 0.7175191162299726 1102 70-71 0.7750506458798441 1102 72-73 0.6710277806851614 1102 74-75 0.7865704702330234 1102 76-77 0.8046860258358528 1102 78-79 0.8692554080177111 1102 80-81 0.6316148623325404 1102 82-83 0.5819688219764778 1102 84-85 0.37841837492987196 1102 86-87 0.46708161278221993 1102 88-89 0.23080167908017302 1102 90-91 0.4361090182760172 1102 92-93 0.006649473100978298 1102 94-95 0.2939065758307642 1102 96-97 0.3129767986068188 1102 98-99 0.3017267721438053 1102 100-101 0.6458594569313121 1103 1 -0.09353351745384941 1103 2 -0.18301149203482936 1103 3 -0.15983908526563795 1103 4 -0.11764720893007308 1103 5 -0.2791626847945139 1103 6 -0.3238477413710896 1103 7 -0.06365220987579079 1103 8 -0.16167216041182542 1103 9 -0.21573836991517936 1103 10-11 -0.24251789663654932 1103 12-13 -0.2972488407484093 1103 14-15 -0.19127864471702338 1103 16-17 -0.055399674903803486 1103 18-19 -0.12721863624413032 1103 20-21 -0.19188675550635992 1103 22-23 -0.09265617712799923 1103 24-25 -0.29600968317731 1103 26-27 -0.21981283367631477 1103 28-29 -0.2685348539556571 1103 30-31 -0.38076392626336997 1103 32-33 -0.5015609701118393 1103 34-35 -0.29450842558156154 1103 36-37 0.03060705743126846 1103 38-39 -0.02345142228809749 1103 40-41 0.11251275844325193 1103 42-43 0.2878160009740398 1103 44-45 -0.10047883044638439 1103 46-47 0.09922441762140011 1103 48-49 0.1920658482914348 1103 50-51 0.07097827924214073 1103 52-53 0.04547082819714632 1103 54-55 -0.018689286995275722 1103 56-57 -0.08899662462754065 1103 58-59 -0.30945879131563814 1103 60-61 -0.14287599922290894 1103 62-63 -0.12673398324241347 1103 64-65 -0.23569853127891705 1103 66-67 -0.11814966450878117 1103 68-69 -0.09103047267034015 1103 70-71 -0.10031548978305693 1103 72-73 0.05496919897909791 1103 74-75 0.17503398513538926 1103 76-77 0.26888823549669993 1103 78-79 0.17752236587999093 1103 80-81 -0.17292008114125323 1103 82-83 -0.14864525818796537 1103 84-85 0.24241503474487658 1103 86-87 0.3183534524533407 1103 88-89 0.3470979277954882 1103 90-91 0.607126490012913 1103 92-93 0.5969500897505142 1103 94-95 0.2396863805584104 1103 96-97 0.32877201957290225 1103 98-99 0.56741279475429 1103 100-101 0.985781091052587 1104 1 0.01930636936685204 1104 2 -0.012303613892896692 1104 3 0.2031435445035612 1104 4 0.10397549290845376 1104 5 0.08695711777613013 1104 6 0.03160440484844429 1104 7 0.09811361555910025 1104 8 0.006885057306064368 1104 9 -0.06110651983968296 1104 10-11 0.008520954821968019 1104 12-13 0.11671681212611418 1104 14-15 0.14900785624094226 1104 16-17 0.1121900628298178 1104 18-19 0.0665485722113246 1104 20-21 0.10310740789307715 1104 22-23 -0.025673255528765537 1104 24-25 0.10631766129631615 1104 26-27 0.01247752451332218 1104 28-29 -0.07687389700692648 1104 30-31 0.03943007726397241 1104 32-33 0.03036111127067187 1104 34-35 0.24032878401583702 1104 36-37 0.21387314297553672 1104 38-39 0.3418071644932752 1104 40-41 0.5911393809547647 1104 42-43 0.6692949448031058 1104 44-45 0.9011389229699276 1104 46-47 1.1491051479577692 1104 48-49 1.4075632916066283 1104 50-51 1.286610535342021 1104 52-53 1.187615842344563 1104 54-55 1.1650541944100752 1104 56-57 1.2888233416216792 1104 58-59 1.3471000885645843 1104 60-61 1.3761849424999042 1104 62-63 1.7227737121885447 1104 64-65 1.7885868023589353 1104 66-67 1.7634926406946754 1104 68-69 1.8673758181700038 1104 70-71 1.8188888293013576 1104 72-73 1.747615082272464 1104 74-75 1.594374703037257 1104 76-77 1.5539474896806524 1104 78-79 1.8812593762716787 1104 80-81 1.9745381427558222 1104 82-83 2.0760384868794723 1104 84-85 2.0452283396565036 1104 86-87 1.9094757926763997 1104 88-89 1.706716582078414 1104 90-91 1.5879608701278691 1104 92-93 1.8106838646353722 1104 94-95 1.8104519902928438 1104 96-97 2.103772653986006 1104 98-99 1.887683562267263 1104 100-101 1.7631544304762876 1105 1 0.021334588061741044 1105 2 -0.08884682376943687 1105 3 -0.27869067066398756 1105 4 0.14286438179734517 1105 5 0.02514053929288451 1105 6 -0.013016406439035677 1105 7 0.007637425082911875 1105 8 -0.05193328484561022 1105 9 0.17469594929611532 1105 10-11 0.13131636928405044 1105 12-13 0.23541169748766322 1105 14-15 0.12444877334852578 1105 16-17 0.00994138558113633 1105 18-19 0.15790659690268427 1105 20-21 -0.03467918822685334 1105 22-23 0.0056318591096768955 1105 24-25 -0.010304913659588522 1105 26-27 -0.16027379823800203 1105 28-29 0.10288800775497009 1105 30-31 0.3729397774403367 1105 32-33 0.2730859789955389 1105 34-35 0.5144910415114339 1105 36-37 0.5613158237515492 1105 38-39 0.34361492463437315 1105 40-41 0.39801768783307523 1105 42-43 0.4303619642034562 1105 44-45 0.6537844256154344 1105 46-47 0.6840698745891558 1105 48-49 0.8694239618006279 1105 50-51 0.7076422813737651 1105 52-53 0.882501203896588 1105 54-55 0.945212924568807 1105 56-57 1.0709221070537893 1105 58-59 0.987796737594568 1105 60-61 1.1080632493782119 1105 62-63 0.9605162165977212 1105 64-65 0.9485074372795701 1105 66-67 0.8580693602713936 1105 68-69 0.6651712326320833 1105 70-71 0.8527953548745515 1105 72-73 1.069837304494687 1105 74-75 0.6947715284340852 1105 76-77 0.5231714755713064 1105 78-79 0.7627408577531618 1105 80-81 0.8916016348193132 1105 82-83 0.9262148537225094 1105 84-85 1.2791789569404557 1105 86-87 1.2662218244224341 1105 88-89 1.1582157002441988 1105 90-91 1.335050817217816 1105 92-93 1.5669007958523018 1105 94-95 1.3481063112804996 1105 96-97 1.1655892324692516 1105 98-99 1.1250733329903682 1105 100-101 1.448604165926021 1106 1 -0.1955211145217497 1106 2 -0.2396134694321148 1106 3 -0.208971424007121 1106 4 -0.00797577783298209 1106 5 0.039804825507260944 1106 6 -0.10209433124474998 1106 7 -0.4497104710173474 1106 8 -0.27946601606453925 1106 9 -0.25513671891084044 1106 10-11 -0.7320614224963862 1106 12-13 -0.34844323494949947 1106 14-15 -0.6061172842024121 1106 16-17 -0.7467781411289351 1106 18-19 -0.592054895780926 1106 20-21 -0.5744379923996448 1106 22-23 -0.42139677633104355 1106 24-25 -0.5716978283133756 1106 26-27 -0.6934566029708975 1106 28-29 -0.3093730718501533 1106 30-31 -0.46630267862632024 1106 32-33 -0.40097584275059717 1106 34-35 -0.281073615773451 1106 36-37 -0.9548922557835695 1106 38-39 -0.8039538634353605 1106 40-41 -0.6289024269887378 1106 42-43 -0.2780133771113995 1106 44-45 -0.3861406601508648 1106 46-47 -0.3733007758344229 1106 48-49 -0.1291320138347416 1106 50-51 -0.681318371150212 1106 52-53 -0.4849636144188949 1106 54-55 -0.5530472448633859 1106 56-57 -0.7144556147133514 1106 58-59 -0.4847913041333598 1106 60-61 -0.8050694958433837 1106 62-63 -0.7128507231208268 1106 64-65 -0.5387203860068546 1106 66-67 -0.28588674138792314 1106 68-69 -0.5883224311640376 1106 70-71 -0.4349260621946911 1106 72-73 -0.629721611699388 1106 74-75 -0.6238467590405357 1106 76-77 -0.5124267880987929 1106 78-79 -0.3517799011910405 1106 80-81 -0.5552775222205248 1106 82-83 -0.5521825417826385 1106 84-85 -0.44726856808930293 1106 86-87 -0.3845150655866689 1106 88-89 -0.3597077052028368 1106 90-91 -0.12832522423006054 1106 92-93 -0.3943800975174412 1106 94-95 -0.46745414520623285 1106 96-97 -0.41422243174694984 1106 98-99 -0.2510311268570078 1106 100-101 -0.3107146046403848 1107 1 -0.08977652304937322 1107 2 -0.2684764533990709 1107 3 -0.09906104103435709 1107 4 -0.218246729313762 1107 5 -0.22300760885526927 1107 6 -0.1444978879205152 1107 7 -0.13125146380598096 1107 8 -0.1408221737344988 1107 9 0.10802928262945244 1107 10-11 -0.18442437145669288 1107 12-13 0.04652280859878033 1107 14-15 -5.512266514742237E-4 1107 16-17 -0.04191046627071415 1107 18-19 -0.07727858828250334 1107 20-21 0.004209700662038074 1107 22-23 0.09359482207264591 1107 24-25 -0.08345306180773093 1107 26-27 -0.18619972416392727 1107 28-29 0.025110229977194365 1107 30-31 -0.08168985218929947 1107 32-33 -0.042654761745197334 1107 34-35 -0.11236081034041945 1107 36-37 -0.26831380587808695 1107 38-39 -0.41657026055082014 1107 40-41 -0.16216749735210811 1107 42-43 0.07017677901826858 1107 44-45 -0.12769705586605085 1107 46-47 0.08036617088545484 1107 48-49 0.07775729513395646 1107 50-51 0.006716355447842659 1107 52-53 -0.0406469442515629 1107 54-55 -0.0816389272830449 1107 56-57 0.09314432927600791 1107 58-59 0.14057451537234655 1107 60-61 0.2904706567856188 1107 62-63 0.20773843881994125 1107 64-65 0.07535928913141987 1107 66-67 0.018254545456581894 1107 68-69 0.16054160300245712 1107 70-71 0.42686942894862767 1107 72-73 0.21242989708727933 1107 74-75 0.13180856547112185 1107 76-77 -0.13979148739165836 1107 78-79 -0.10299988298757867 1107 80-81 0.033268301485978924 1107 82-83 0.24195559446324566 1107 84-85 0.08751229027378926 1107 86-87 -0.12081521261460537 1107 88-89 -0.20289541086691543 1107 90-91 -0.1436528864858886 1107 92-93 -0.36458068562917845 1107 94-95 -0.12781961464542846 1107 96-97 0.025774417654435666 1107 98-99 0.3908140737311072 1107 100-101 0.9050856474075033 1108 1 0.014776224644172942 1108 2 -0.008180959349061823 1108 3 -0.022921182764534365 1108 4 -0.12245852233422028 1108 5 -0.0803080500907285 1108 6 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-2.0120904330453726 2316 58-59 -1.9058092699878166 2316 60-61 -2.190077608014615 2316 62-63 -2.28156602312232 2316 64-65 -2.4445298913876776 2316 66-67 -2.929222853201921 2316 68-69 -2.7681037746586696 2316 70-71 -2.784292717449741 2316 72-73 -2.633837236187727 2316 74-75 -2.5425352665498764 2316 76-77 -2.9005234792260133 2316 78-79 -2.747568270272506 2316 80-81 -2.7772012231567764 2316 82-83 -2.80755592493016 2316 84-85 -2.4730228511668635 2316 86-87 -3.0811607944168777 2316 88-89 -2.928471380537374 2316 90-91 -2.7329573484281475 2316 92-93 -2.738049627011506 2316 94-95 -2.8712579348554 2316 96-97 -2.569566789694644 2316 98-99 -2.6148508431542865 2316 100-101 -2.3847437489179555 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1858.0 3 312.0 4 175.0 5 173.0 6 209.0 7 268.0 8 333.0 9 402.0 10 633.0 11 1136.0 12 2316.0 13 3844.0 14 4140.0 15 6523.0 16 9512.0 17 13682.0 18 10681.0 19 5362.0 20 4531.0 21 4420.0 22 4562.0 23 5044.0 24 5472.0 25 6008.0 26 6595.0 27 7560.0 28 8467.0 29 10150.0 30 12046.0 31 13786.0 32 17163.0 33 22310.0 34 31706.0 35 53178.0 36 55373.0 37 75094.0 38 69867.0 39 9616.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.14148297920336 22.200607198216897 16.440502231981174 16.21740759059857 2 14.442079219186047 25.164824480288296 41.161953110616004 19.23114318990966 3 17.203582657620387 34.07661757405263 31.99031937779249 16.729480390534505 4 9.73161720595597 20.569532533191012 40.22659666455667 29.472253596296344 5 8.735198960720876 43.093675787940725 33.0930965718683 15.078028679470101 6 21.91399856395273 42.457130678232296 18.781387668616574 16.847483089198402 7 21.675206105353773 40.97399264973678 22.0876237459855 15.263177498923946 8 25.611078237049114 33.286642969755405 20.606174714374976 20.496104078820508 9 28.824545614416202 16.663046064405343 22.187464440513505 32.32494388066495 10-11 22.20319711471312 31.400980178137083 25.863708268196767 20.532114438953027 12-13 25.848130130424256 31.01285205548049 27.707239653277988 15.431778160817267 14-15 22.46462117763893 30.27961683287127 30.683783671949357 16.57197831754045 16-17 15.47312355281863 34.278049194337704 30.917277612582694 19.331549640260974 18-19 14.779435040872949 29.818011408425356 39.56803571908873 15.834517831612965 20-21 17.027626149624144 28.336172805408754 39.97469818325508 14.66150286171202 22-23 16.529225689394426 29.09152410527165 39.84647022192615 14.532779983407778 24-25 15.672428363227889 30.020676736447637 39.83928207557611 14.467612824748368 26-27 14.650275829894726 30.198227199424032 40.74255290317041 14.408944067510829 28-29 14.376104381939243 30.39921208685253 40.67477477850288 14.549908752705349 30-31 14.747273688468537 30.798086900565796 39.847237582492845 14.60740182847282 32-33 13.963547804064017 31.69698142214672 39.942229363084934 14.397241410704329 34-35 14.385157533594613 31.51458215212452 39.65892207539595 14.441338238884912 36-37 14.574474137440587 31.304945978515537 38.98287552560396 15.137704358439919 38-39 13.895253394680848 32.39467360688008 39.07159474229182 14.63847825614725 40-41 13.740454067293978 32.34223545754794 39.33167351591325 14.585636959244827 42-43 13.837063518663339 32.41775179268197 37.8008664457106 15.944318242944094 44-45 14.151231326799502 32.333049508617684 37.4219266490716 16.09379251551121 46-47 14.746087485540821 32.832116244565526 36.765944414669626 15.655851855224032 48-49 14.535008241067521 33.19158490265401 35.69618655335069 16.577220302927778 50-51 14.466724608889992 33.36840907209668 35.22007698038242 16.94478933863091 52-53 14.841393871024497 34.092089875081506 34.2964925535328 16.770023700361197 54-55 15.11649923899649 33.57127410922237 33.952862273367366 17.35936437841378 56-57 15.593877103478523 33.81774886938676 33.212927677991416 17.3754463491433 58-59 15.480279276512086 33.16455120190046 33.87060243430993 17.48456708727753 60-61 16.01353964783678 33.90217366573658 33.83329644713518 16.250990239291454 62-63 16.044258597133204 33.921041553974035 34.248595716249355 15.786104132643406 64-65 16.264470878294667 33.81009409044183 34.19501893598582 15.730416095277691 66-67 16.263895826675643 33.90845430937392 33.606156410980354 16.22149345297008 68-69 15.925278957661732 33.789118453453966 34.12917324695445 16.15642934192985 70-71 16.41634363584463 33.654399079931 34.008422933878315 15.92083435034606 72-73 15.770448431467257 33.54904903403702 34.09369928810716 16.586803246388563 74-75 15.002699499196357 34.4552286506848 33.66006582227159 16.88200602784725 76-77 14.534762234796371 33.707833786761285 34.69944450766 17.057959470782343 78-79 14.260948537040235 33.37319101795354 34.76876164311581 17.59709880189041 80-81 14.704078392514896 33.750091795119495 33.86386602773816 17.681963784627445 82-83 14.93799107849292 32.969757270056064 33.86645871191475 18.22579293953626 84-85 14.706858836123308 32.70118063204927 33.60431502138376 18.987645510443656 86-87 14.595340446923213 32.81039897061196 33.77482964207251 18.81943094039231 88-89 14.967666662529325 32.11654068456482 33.55612926715239 19.35966338575347 90-91 15.009966578086356 31.511351141113714 33.999530376081864 19.479151904718066 92-93 15.657470403644028 31.69607857333463 33.771391809623495 18.875059213397847 94-95 15.385522367837229 30.567188322575106 34.321518073510354 19.72577123607731 96-97 15.451938771921478 30.43850194363359 34.251602799494385 19.857956484950545 98-99 15.622006649247048 30.50450696474492 33.995465105934876 19.878021280073156 100-101 16.138466820831248 29.246937737090096 34.86491294113754 19.74968250094112 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4083.0 1 2821.0 2 2030.0 3 2444.0 4 2034.0 5 2152.5 6 3959.5 7 7940.0 8 11751.0 9 11909.5 10 8503.5 11 5565.0 12 4975.0 13 4750.5 14 4303.5 15 3819.0 16 3500.5 17 3265.0 18 3014.0 19 2941.0 20 2974.5 21 2934.5 22 2981.5 23 3212.5 24 3373.5 25 3796.0 26 4764.5 27 5726.5 28 5979.5 29 6346.5 30 7928.0 31 9488.0 32 11552.0 33 14553.0 34 16904.5 35 18793.0 36 22152.0 37 24528.5 38 23581.5 39 21606.5 40 19128.5 41 16600.0 42 14259.5 43 13823.0 44 13835.0 45 12797.5 46 11176.5 47 10695.0 48 12998.0 49 11734.5 50 7789.0 51 5237.0 52 3516.5 53 2915.5 54 2571.5 55 2353.5 56 2276.5 57 2078.0 58 1913.0 59 1780.5 60 1601.0 61 1653.0 62 1485.0 63 837.5 64 423.0 65 378.5 66 353.0 67 257.0 68 187.5 69 135.0 70 102.0 71 105.0 72 99.5 73 112.5 74 111.5 75 75.5 76 49.5 77 45.0 78 43.5 79 29.0 80 16.5 81 7.0 82 7.0 83 5.5 84 1.5 85 2.0 86 2.0 87 1.0 88 0.5 89 0.5 90 1.0 91 1.0 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26913955835519404 2 0.22992443865620105 3 0.22022385641487122 4 0.22559013595262814 5 0.22600292668630176 6 0.2551046734102913 7 0.2598517668475378 8 0.243752928234267 9 0.2398314162643677 10-11 0.25335031279217846 12-13 0.2555174641439649 14-15 0.24086339309855173 16-17 0.2559302548776385 18-19 0.24354653286743022 20-21 0.23580670661105 22-23 0.23694188112865244 24-25 0.22982124097278264 26-27 0.236838683445234 28-29 0.2487064170383503 30-31 0.24922240545544233 32-33 0.2510799637569736 34-35 0.25696223171182253 36-37 0.23415554367635555 38-39 0.23539391587737638 40-41 0.23126600854064028 42-43 0.23797385796283646 44-45 0.25335031279217846 46-47 0.2579942085460066 48-49 0.2593357784304458 50-51 0.2615029297822322 52-53 0.2873023506368329 54-55 0.2607805459983034 56-57 0.2354971135607948 58-59 0.2153735652942063 60-61 0.21423839077660384 62-63 0.22197821703298404 64-65 0.21382560004293022 66-67 0.2153735652942063 68-69 0.21785030969624794 70-71 0.2198110656811976 72-73 0.22239100776665766 74-75 0.2237325776510969 76-77 0.23776746259599962 78-79 0.23126600854064028 80-81 0.22569333363604654 82-83 0.22053344946512643 84-85 0.22280379850033127 86-87 0.22971804328936427 88-89 0.2280668803546698 90-91 0.23560031124421318 92-93 0.2241453683847705 94-95 0.22259740313349446 96-97 0.23405234599293717 98-99 0.2361162996613052 100-101 0.21485757687711424 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 484507.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.7895783569797 #Duplication Level Percentage of deduplicated Percentage of total 1 76.35958879435529 22.747199536961965 2 10.226287669167352 6.092735956433524 3 3.857137317450526 3.4470748305546883 4 1.9943060081771442 2.3763814039355378 5 1.2353734466681192 1.840062704482601 6 0.8358961193978569 1.494059576825863 7 0.6252150894928312 1.3037425728888938 8 0.4939995833225312 1.1772831436561493 9 0.38111905653494277 1.021803839818727 >10 3.219285651628451 19.64429803953276 >50 0.47510806293366636 9.803662382232343 >100 0.278702400818439 14.716103374611148 >500 0.009681969259201353 1.8539340109212359 >1k 0.005532553862400774 3.363822039151232 >5k 0.0013831384656001935 3.293982764820495 >10k+ 0.0013831384656001935 5.823853823172843 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16068 3.3163607543337865 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12201 2.5182298707758606 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9856 2.0342327355435526 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6133 1.2658227848101267 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3200 0.6604651738777768 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2693 0.5558227228915166 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2381 0.4914273684384333 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2118 0.4371453869603535 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1681 0.3469506116526696 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1612 0.33270933134093006 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1422 0.29349421164193706 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1221 0.25200874290773917 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 923 0.19050292359037124 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 900 0.18575583015312472 No Hit CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA 754 0.15562210659495115 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 747 0.15417733902709352 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 699 0.14427036141892688 No Hit GTACATGGGAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG 674 0.13911047724800674 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 611 0.12610756913728802 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 587 0.12115408033320468 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 529 0.10918314905666998 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 521 0.10753198612197554 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 521 0.10753198612197554 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 513 0.10588082318728109 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 511 0.10546803245360747 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 509 0.10505524171993386 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 492 0.10154652048370819 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.25581467347221E-4 0.0 24-25 0.0 0.0 0.0 0.0039215119698993 0.0 26-27 0.0 0.0 0.0 0.008359012356890613 0.0 28-29 0.0 0.0 0.0 0.01620203629668921 0.0 30-31 0.0 0.0 0.0 0.03488081699542009 0.0 32-33 0.0 0.0 0.0 0.05995785406609192 0.0 34-35 0.0 0.0 0.0 0.08967878689059189 0.0 36-37 0.0 0.0 0.0 0.12703634828805363 0.0 38-39 0.0 0.0 0.0 0.1762616432786317 0.0 40-41 0.0 0.0 0.0 0.2394186255306941 0.0 42-43 0.0 0.0 0.0 0.30040845643097003 0.0 44-45 0.0 0.0 0.0 0.3856497429345706 0.0 46-47 0.0 0.0 0.0 0.4832747514483795 0.0 48-49 0.0 0.0 0.0 0.5919419120879574 0.0 50-51 0.0 0.0 0.0 0.7245509352806048 0.0 52-53 0.0 0.0 0.0 0.8728460063528494 0.0 54-55 0.0 0.0 0.0 1.0315640434503526 0.0 56-57 0.0 0.0 0.0 1.2313547585483802 0.0 58-59 0.0 0.0 0.0 1.4527137894808537 0.0 60-61 0.0 0.0 0.0 1.7054449161725218 0.0 62-63 0.0 0.0 0.0 1.96591586912057 0.0 64-65 0.0 0.0 0.0 2.2297923456214255 0.0 66-67 0.0 0.0 0.0 2.5457836522485744 0.0 68-69 0.0 0.0 0.0 2.8954174036701223 0.0 70-71 0.0 0.0 0.0 3.295102031549596 0.0 72-73 0.0 0.0 0.0 3.687872414640036 0.0 74-75 0.0 0.0 0.0 4.110466928238395 0.0 76-77 0.0 0.0 0.0 4.541317256510226 0.0 78-79 0.0 0.0 0.0 5.045953928426215 0.0 80-81 0.0 0.0 0.0 5.553067344744245 0.0 82-83 0.0 0.0 0.0 6.089488903153102 0.0 84-85 0.0 0.0 0.0 6.651503487049723 0.0 86-87 0.0 0.0 0.0 7.229410514192777 0.0 88-89 0.0 0.0 0.0 7.842817544431763 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTGGTC 25 0.005018542 57.0028 8 AGATAGT 65 4.3997716E-8 51.172253 6 GGGATAC 40 5.381006E-4 47.50725 7 GATAGTC 75 1.358967E-7 44.340103 7 ACTGGGC 55 4.9575814E-5 43.183937 8 GAGCACT 60 8.266065E-5 39.597576 4 TAGATAG 85 3.6216625E-7 39.135777 5 GGTATCA 6025 0.0 38.02155 1 TTCGCGA 25 0.0016111939 38.02155 48-49 GTTCGAC 45 5.708389E-7 36.934784 16-17 TCTAGAT 175 0.0 35.2911 3 CGTCACT 55 0.002570991 34.56505 1 GAGGCGC 110 6.9952875E-8 34.54715 9 GTATCAA 9330 0.0 33.97692 1 GGATGAC 170 3.6379788E-12 33.53106 8 AAGTTCG 50 1.3005138E-6 33.244747 14-15 CGACCGT 50 1.3015651E-6 33.241306 18-19 GGAGGCG 115 1.0338408E-7 33.045097 8 CGCCACT 30 0.003936697 31.68955 50-51 AGGGGTC 105 1.8917981E-6 31.681343 5 >>END_MODULE