FastQCFastQC Report
Thu 26 May 2016
SRR937184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484507
Sequences flagged as poor quality0
Sequence length101
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153963.1776630678194535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130872.70109616579327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104902.165087398118087No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80651.6645786335388344No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37610.7762529746732245No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32740.6757384310237003No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24990.5157820217251763No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21530.4443692247996417No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20310.4191889900455515No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19600.40453491900013827No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.331677354506746No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.19834594753016985No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9530.19669478459547537No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7140.14736629192147896No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6550.13518896527810745No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6450.13312501160973939No Hit
GTACATGGGAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG6380.13168024404188175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6280.1296162903735137No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.12301163863473594No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5580.11516861469493733No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5550.1145494285944269No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5260.10856396295615955No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5240.10815117222248594No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA5220.10773838148881235No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA5190.10711919538830192No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT5130.10588082318728109No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4890.10092733438319776No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG504.2365355E-757.0005573
ATGGGCG352.7805174E-454.3086667
ACTGTGC405.373862E-447.520088
CTCGAGT459.599559E-442.2400749
GTATGGT608.252987E-539.6082461
TCTCGAG500.001611542838.0160688
CTGTGCC651.3237535E-436.553919
GGTATCA72950.035.4438171
TAGATAT1151.0304575E-733.057457
GAGGCGC902.4031484E-531.6800569
ACTGGGC753.0682218E-431.6800548
ACCTGCG300.003950460331.66697394-95
CCACGCG300.003950460331.66697384-85
GTATCAA107100.030.5771261
TGGGGAC1401.7031198E-830.5328626
CATGGAT1252.1493724E-730.40034
GTAGATA1252.1513188E-730.397166
GGATATG953.4766705E-530.0126869
GTGGTAC804.4733277E-429.7061861
AGGACAG804.4895886E-429.687795
Produced by FastQC (version 0.11.5)