##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937184_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 484507 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.729432185706294 31.0 31.0 34.0 30.0 34.0 2 31.900750659949185 31.0 31.0 34.0 30.0 34.0 3 31.828929200197315 31.0 31.0 34.0 30.0 34.0 4 35.69462773499661 37.0 35.0 37.0 33.0 37.0 5 35.6490845333504 37.0 35.0 37.0 33.0 37.0 6 35.74331433807974 37.0 35.0 37.0 33.0 37.0 7 35.654004998895786 37.0 35.0 37.0 33.0 37.0 8 35.666593052319165 37.0 35.0 37.0 33.0 37.0 9 37.21440763497741 39.0 37.0 39.0 33.0 39.0 10-11 37.13377206108477 39.0 37.0 39.0 33.0 39.0 12-13 37.01610193454377 39.0 37.0 39.0 33.0 39.0 14-15 37.78307434154718 39.5 37.0 41.0 32.0 41.0 16-17 37.836543125279924 39.0 37.0 41.0 32.5 41.0 18-19 38.08430528351499 40.0 37.0 41.0 33.0 41.0 20-21 38.14105884951095 40.0 37.0 41.0 33.0 41.0 22-23 37.95915229294933 40.0 37.0 41.0 33.0 41.0 24-25 37.80928242522812 40.0 36.0 41.0 33.0 41.0 26-27 37.57683273925867 39.0 36.0 41.0 33.0 41.0 28-29 37.336026104885995 39.0 35.5 41.0 32.0 41.0 30-31 36.93108355503635 38.5 35.0 40.0 32.0 41.0 32-33 36.441543672227645 38.0 35.0 40.0 31.5 41.0 34-35 36.09934634587323 38.0 35.0 40.0 31.0 41.0 36-37 35.712311690027185 38.0 35.0 40.0 30.0 41.0 38-39 35.4248627986799 38.0 35.0 40.0 30.0 41.0 40-41 35.06437265096273 37.0 35.0 40.0 28.5 41.0 42-43 34.53466100592974 37.0 34.0 40.0 25.5 41.0 44-45 33.796726363086606 37.0 33.0 40.0 22.0 41.0 46-47 32.90029659014215 36.5 33.0 40.0 13.0 41.0 48-49 32.17183859056732 36.0 33.0 40.0 2.0 41.0 50-51 31.78078541692896 36.0 32.5 40.0 2.0 41.0 52-53 31.50111247102725 35.0 31.5 40.0 2.0 41.0 54-55 31.2516785103208 35.0 31.0 40.0 2.0 41.0 56-57 30.924607900401853 35.0 31.0 39.5 2.0 41.0 58-59 30.645135158005974 35.0 31.0 39.0 2.0 41.0 60-61 30.308513602486652 35.0 30.0 39.0 2.0 40.0 62-63 29.91567820485566 35.0 28.5 39.0 2.0 40.0 64-65 29.491524374260848 35.0 28.0 38.0 2.0 40.0 66-67 29.039335860988594 35.0 27.5 38.0 2.0 40.0 68-69 28.568670834477107 34.5 26.0 37.5 2.0 40.0 70-71 28.158503385915992 34.0 26.0 37.0 2.0 39.0 72-73 27.653131946494064 34.0 25.5 36.0 2.0 39.0 74-75 27.12690425525328 33.5 24.0 36.0 2.0 39.0 76-77 25.936192872342403 32.0 22.5 35.0 2.0 37.0 78-79 26.43676252355487 33.0 22.5 35.0 2.0 37.0 80-81 26.13519206120861 33.0 21.0 35.0 2.0 37.0 82-83 25.68628213833856 33.0 19.0 35.0 2.0 36.5 84-85 25.1845504812108 33.0 14.0 35.0 2.0 36.0 86-87 24.65313091451723 32.5 4.5 35.0 2.0 35.5 88-89 24.00712373608637 32.0 2.0 35.0 2.0 35.0 90-91 23.331011729448697 31.0 2.0 34.0 2.0 35.0 92-93 22.86925988685406 31.0 2.0 34.0 2.0 35.0 94-95 22.28045002445785 31.0 2.0 34.0 2.0 35.0 96-97 21.647446786114543 30.0 2.0 34.0 2.0 35.0 98-99 20.803837715450964 29.5 2.0 34.0 2.0 35.0 100-101 18.72593172028474 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2683060138692781 1101 2 -0.2264780232752237 1101 3 -0.2100670952639483 1101 4 -0.22860553875882772 1101 5 -0.20823928593183894 1101 6 -0.2970956277646195 1101 7 0.0010145378133117333 1101 8 -0.18025252437460892 1101 9 -0.21526311315437852 1101 10-11 -0.2655380550394 1101 12-13 -0.29495682627420905 1101 14-15 -0.42859130759297415 1101 16-17 -0.3891714937264297 1101 18-19 -0.3301118363404072 1101 20-21 -0.4146172597404316 1101 22-23 -0.18973266834667157 1101 24-25 -0.33505865011377267 1101 26-27 -0.43346337878148944 1101 28-29 -0.5768322215809079 1101 30-31 -0.3389599980245137 1101 32-33 -0.17082417949801965 1101 34-35 -0.4711776211807077 1101 36-37 0.1269461273830359 1101 38-39 0.04475311781738611 1101 40-41 0.2637885128567632 1101 42-43 0.41387837429205376 1101 44-45 0.7493061937508187 1101 46-47 0.5118209152342601 1101 48-49 0.26917176568586143 1101 50-51 0.4568379525735544 1101 52-53 0.6380942439567576 1101 54-55 0.41088596837611746 1101 56-57 0.5417488345377954 1101 58-59 0.6583959748926347 1101 60-61 0.4281591360512138 1101 62-63 0.2114690517743405 1101 64-65 0.5243080773173041 1101 66-67 0.6065702085171978 1101 68-69 0.2393169910882058 1101 70-71 0.6712875609023996 1101 72-73 1.199268660363355 1101 74-75 0.8361851030501306 1101 76-77 0.5989319976055327 1101 78-79 0.6340971489603646 1101 80-81 0.6812575765028903 1101 82-83 0.8645705341444803 1101 84-85 0.6413402000173711 1101 86-87 0.6320344311269288 1101 88-89 0.7666684067477796 1101 90-91 0.6892800395855865 1101 92-93 0.8171120347963878 1101 94-95 1.1897331315153217 1101 96-97 0.8997975235690241 1101 98-99 1.256984899356727 1101 100-101 1.6924704929016663 1102 1 -0.08554587277580339 1102 2 -0.05723199152919278 1102 3 -0.1505212398847604 1102 4 -0.06928014193343301 1102 5 -0.08101265453854012 1102 6 -2.702309392219604E-4 1102 7 0.08778702458579346 1102 8 -0.08818903231111364 1102 9 -0.02549239005032433 1102 10-11 -0.31323426315228176 1102 12-13 -0.18095770810842282 1102 14-15 4.1001515834437896E-4 1102 16-17 -0.047394597782862036 1102 18-19 -0.12939534604058878 1102 20-21 -0.010660028699867041 1102 22-23 -0.0408129152602541 1102 24-25 -3.364278915469754E-4 1102 26-27 -0.2806415092929484 1102 28-29 -0.5153793997114136 1102 30-31 -0.14022763470881472 1102 32-33 -0.33588149872201 1102 34-35 -0.4485916423447307 1102 36-37 -0.4043038726169641 1102 38-39 -0.4250440603131338 1102 40-41 -0.11600646068820453 1102 42-43 -0.28180063805362465 1102 44-45 -0.619576081381453 1102 46-47 -0.409640724977379 1102 48-49 0.2963763512237776 1102 50-51 0.4496069120091839 1102 52-53 0.31193683654934645 1102 54-55 0.40797591546606427 1102 56-57 0.34592652413214964 1102 58-59 0.3265838055804693 1102 60-61 0.19808858931400053 1102 62-63 0.42046376076904934 1102 64-65 0.18927721311977663 1102 66-67 0.29808255419620977 1102 68-69 0.24908330502118758 1102 70-71 0.6098347390329124 1102 72-73 0.49244546812349554 1102 74-75 0.32937293373796095 1102 76-77 0.34300166250852726 1102 78-79 0.34578145231133206 1102 80-81 0.15444981636179733 1102 82-83 0.4664003401409538 1102 84-85 0.23944425645476386 1102 86-87 0.18786555987472653 1102 88-89 0.3860908053368455 1102 90-91 0.2854440607496116 1102 92-93 0.4148633575477092 1102 94-95 0.26848092692978653 1102 96-97 0.24232397859548271 1102 98-99 0.13284468771651348 1102 100-101 1.0848646727958489 1103 1 -0.09338246064836042 1103 2 -0.16441338926814453 1103 3 -0.1600150733893848 1103 4 -0.08100933001153976 1103 5 -0.46704811188695317 1103 6 -0.1926648866432359 1103 7 0.012196068779012137 1103 8 -0.1576134927427688 1103 9 -0.2792436747368612 1103 10-11 -0.22887532173398029 1103 12-13 -0.26997363822147946 1103 14-15 -0.196024979702905 1103 16-17 -0.23479182285995392 1103 18-19 -0.11700454439618824 1103 20-21 -0.46282472551384757 1103 22-23 -0.05398095225923072 1103 24-25 -0.11309336519884994 1103 26-27 -0.17231674053652313 1103 28-29 -0.18833032468572242 1103 30-31 -0.43654700778178324 1103 32-33 -0.10113072791008193 1103 34-35 0.027570998970794847 1103 36-37 -0.013470111054786571 1103 38-39 0.031918940714618316 1103 40-41 0.27212945914735087 1103 42-43 0.4370328665712435 1103 44-45 0.22622941707692945 1103 46-47 0.34578707563927225 1103 48-49 0.31608344269346134 1103 50-51 0.38771071468132945 1103 52-53 0.5151164357271512 1103 54-55 0.4075648157743892 1103 56-57 0.1635973874415022 1103 58-59 0.20324105121253666 1103 60-61 0.1621687537538783 1103 62-63 0.266558313698134 1103 64-65 0.11261996748770997 1103 66-67 0.24588574021882081 1103 68-69 0.07003654243125723 1103 70-71 -0.06420816949740527 1103 72-73 0.07104901385833884 1103 74-75 0.37396440341935744 1103 76-77 0.6372077262289793 1103 78-79 0.6635101265148258 1103 80-81 0.5151127146146237 1103 82-83 0.22884895267949545 1103 84-85 0.043401090987135404 1103 86-87 0.2575276873156298 1103 88-89 0.6356668587798069 1103 90-91 0.5090052118287467 1103 92-93 0.5975971704973517 1103 94-95 0.8270883267242368 1103 96-97 0.8728095901062716 1103 98-99 1.3563672981995545 1103 100-101 1.5244715587156854 1104 1 -0.061956807520068935 1104 2 -0.058929522393391665 1104 3 -0.14128402648088212 1104 4 -0.03107467456129598 1104 5 -0.030108774467997534 1104 6 -0.09074642141541744 1104 7 -0.13302426289215674 1104 8 -0.16790684536226053 1104 9 -0.04595094384220744 1104 10-11 -0.06624352241153986 1104 12-13 -0.2420467733641587 1104 14-15 -0.376563088898088 1104 16-17 -0.3750621462837458 1104 18-19 -0.12552629842154062 1104 20-21 -0.23207757720074795 1104 22-23 -0.29731644259711487 1104 24-25 -0.1057817541702164 1104 26-27 -0.1292305745486786 1104 28-29 -0.08035955844157883 1104 30-31 -0.11673777580228517 1104 32-33 -0.025321533995374068 1104 34-35 0.2051892218527982 1104 36-37 0.0043711714747445285 1104 38-39 -0.031084624687025553 1104 40-41 0.08742166982325728 1104 42-43 -0.34802638761270543 1104 44-45 -0.15281020836557246 1104 46-47 0.04356694698029884 1104 48-49 0.004621501135595452 1104 50-51 0.0785310742666816 1104 52-53 0.15661276247526956 1104 54-55 0.1816090724325541 1104 56-57 0.13522326134555485 1104 58-59 0.12224077207076789 1104 60-61 0.2976476722064163 1104 62-63 0.08624859322054945 1104 64-65 0.03312641946898154 1104 66-67 0.38258431786464087 1104 68-69 0.5250312768024905 1104 70-71 0.43848332809816526 1104 72-73 0.41002703778839944 1104 74-75 0.36616746636582675 1104 76-77 0.40404663605350066 1104 78-79 0.26637228123549406 1104 80-81 0.2376949662736152 1104 82-83 0.5444647140386571 1104 84-85 0.8247617167722261 1104 86-87 0.5949973940898907 1104 88-89 0.5356278423738843 1104 90-91 0.36388321418876046 1104 92-93 0.23333778435547003 1104 94-95 0.732943008058534 1104 96-97 0.5276634847683219 1104 98-99 0.6758561515613088 1104 100-101 1.0081671419316507 1105 1 0.0444805822506531 1105 2 -0.04975844655564643 1105 3 0.01726976540624392 1105 4 0.029595519442231932 1105 5 -0.12578778681367453 1105 6 -0.15926493993393365 1105 7 0.013448400247170866 1105 8 -0.01596681008889078 1105 9 0.05889914434121124 1105 10-11 -0.05579378696180015 1105 12-13 0.11725657760585761 1105 14-15 0.14746689346522857 1105 16-17 0.16470857682030982 1105 18-19 0.2316165587213206 1105 20-21 0.10875134696150468 1105 22-23 -0.10304836499570769 1105 24-25 -0.10582584588096466 1105 26-27 0.33628970763826516 1105 28-29 0.22479917171715869 1105 30-31 0.33705411132293506 1105 32-33 0.3189465436060388 1105 34-35 0.14257899257590623 1105 36-37 -0.15612265568574912 1105 38-39 0.24413583386677118 1105 40-41 -0.03726968820144094 1105 42-43 0.0716385153855299 1105 44-45 0.07642259586722133 1105 46-47 0.06645054486389057 1105 48-49 -0.08642788158045533 1105 50-51 0.16151167391394594 1105 52-53 0.23663921750172534 1105 54-55 0.18734099483114264 1105 56-57 0.17393578339141058 1105 58-59 0.356411847908511 1105 60-61 0.25761239883781073 1105 62-63 0.5355431258484131 1105 64-65 0.5150047263472892 1105 66-67 0.3071434007570595 1105 68-69 0.5208425642804464 1105 70-71 0.7351323580805307 1105 72-73 0.4248859443139743 1105 74-75 0.6181956850607122 1105 76-77 0.49002547203233604 1105 78-79 0.2574216639515434 1105 80-81 0.33989955181153064 1105 82-83 0.4249320861726993 1105 84-85 0.5014812934917998 1105 86-87 0.6214965122556784 1105 88-89 0.7931675249135672 1105 90-91 0.8400736903792385 1105 92-93 1.2176411353254863 1105 94-95 1.4220523555012115 1105 96-97 1.2724165711880708 1105 98-99 1.2956092379810649 1105 100-101 1.4174704929016677 1106 1 -0.3360522189815818 1106 2 -0.2020233768924129 1106 3 0.01600252463049756 1106 4 -0.2692632637265717 1106 5 -0.3072447636392752 1106 6 -0.3207401202181899 1106 7 -0.22217786874393397 1106 8 -0.43866162210317583 1106 9 -0.38638896039869053 1106 10-11 -0.6726337365522284 1106 12-13 -0.423527921448958 1106 14-15 -0.4420538654789752 1106 16-17 -0.36094779789088705 1106 18-19 -0.30304002602978386 1106 20-21 -0.19190327741713276 1106 22-23 -0.2440096476394089 1106 24-25 -0.13052478868009842 1106 26-27 -0.4554592246588669 1106 28-29 -0.6838492214775513 1106 30-31 -0.39890472085517814 1106 32-33 -0.5333463920517332 1106 34-35 -0.5340645697009236 1106 36-37 -0.515640964164362 1106 38-39 -0.5846781407884194 1106 40-41 -0.4572584860730302 1106 42-43 -0.5084952100223035 1106 44-45 -0.6489694786269311 1106 46-47 -1.0246910220338172 1106 48-49 -1.0161607808313136 1106 50-51 -1.2462072926897108 1106 52-53 -0.9718516457622925 1106 54-55 -1.0741061801321017 1106 56-57 -1.0364576913687955 1106 58-59 -1.2388394998475647 1106 60-61 -1.1171828122522953 1106 62-63 -0.9102140680184192 1106 64-65 -1.111651088257485 1106 66-67 -0.9524676753474033 1106 68-69 -1.3090688688918561 1106 70-71 -1.3607718637216095 1106 72-73 -1.4348651583955423 1106 74-75 -1.3664784030160213 1106 76-77 -1.2007432740294135 1106 78-79 -1.2922755285150274 1106 80-81 -1.2747157250910774 1106 82-83 -1.2523783528107089 1106 84-85 -1.1884858002560108 1106 86-87 -1.1702646723694023 1106 88-89 -0.5755382791892139 1106 90-91 -0.5145086802711845 1106 92-93 -0.5189268125846169 1106 94-95 -0.6688266615120178 1106 96-97 -0.1083140176238011 1106 98-99 -0.06038040005016043 1106 100-101 0.6378251102789712 1107 1 -0.14626015849008667 1107 2 -0.04790659470379666 1107 3 0.07282532096180105 1107 4 -0.0407748509281447 1107 5 0.03347147244558357 1107 6 0.042586911917915415 1107 7 -0.3235886367898644 1107 8 -0.08078162490370744 1107 9 -0.1336934482513783 1107 10-11 -0.043756749924767746 1107 12-13 -0.08274342239413812 1107 14-15 -0.060866439868107136 1107 16-17 0.024893762005490316 1107 18-19 -0.06930936720461034 1107 20-21 -0.09310050489035149 1107 22-23 -0.054900216847563854 1107 24-25 -0.033603623658748916 1107 26-27 -0.18037695902840056 1107 28-29 0.056280653198633956 1107 30-31 0.01668374095256553 1107 32-33 0.08190950656899787 1107 34-35 -0.1731617481648371 1107 36-37 -0.3792708038339043 1107 38-39 -0.5577160179850864 1107 40-41 -0.4715289474606976 1107 42-43 -0.4903985216515139 1107 44-45 -0.5096885152438873 1107 46-47 -0.4863272329138866 1107 48-49 -0.1216130667656401 1107 50-51 -0.014414252011981432 1107 52-53 -0.3087311528686456 1107 54-55 -0.3191404866503369 1107 56-57 -0.037175327719701556 1107 58-59 -0.2935881520914876 1107 60-61 -0.05998019375478236 1107 62-63 -0.13853094822566092 1107 64-65 -0.07758786624530245 1107 66-67 -0.1113751177614617 1107 68-69 0.3078796013174845 1107 70-71 0.6193916173397866 1107 72-73 0.38507112949915623 1107 74-75 0.27745494431997386 1107 76-77 0.3224328794397451 1107 78-79 0.4083475898774722 1107 80-81 0.5889736258856075 1107 82-83 0.5527098639504757 1107 84-85 0.6709257379362477 1107 86-87 0.3955705863297503 1107 88-89 0.566315673061716 1107 90-91 0.5752588755644226 1107 92-93 0.3639374316217854 1107 94-95 0.5276079110567693 1107 96-97 0.9113054600769637 1107 98-99 1.07431294168477 1107 100-101 1.4980260484572234 1108 1 -0.13434680108936448 1108 2 -0.16172528704234423 1108 3 -0.07456069321121817 1108 4 -0.26112516514127293 1108 5 -0.02406964267675704 1108 6 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-0.29330306384933635 2316 50-51 -0.1052862263485217 2316 52-53 -0.037310913731865014 2316 54-55 -0.22385760534383436 2316 56-57 -0.03571717665525043 2316 58-59 -0.09274971522943076 2316 60-61 -0.1850385197973594 2316 62-63 -0.7417972066100305 2316 64-65 -0.5756412345742632 2316 66-67 -0.4180169958600324 2316 68-69 -0.7315269311992871 2316 70-71 -0.6096182980874474 2316 72-73 -0.7051009323264488 2316 74-75 -1.2192350527637252 2316 76-77 -1.5880585174689728 2316 78-79 -1.9080711911082417 2316 80-81 -1.7148977651905106 2316 82-83 -1.4047048342122537 2316 84-85 -1.5705951136239769 2316 86-87 -1.2363118866944518 2316 88-89 -1.2469097570928476 2316 90-91 -1.5297134195653506 2316 92-93 -1.3876956975704005 2316 94-95 -2.3159397704830376 2316 96-97 -2.5723413757352276 2316 98-99 -3.0125855710011464 2316 100-101 -2.676004281084918 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 10.0 5 31.0 6 76.0 7 180.0 8 435.0 9 747.0 10 1220.0 11 2131.0 12 3281.0 13 4392.0 14 5591.0 15 7913.0 16 11058.0 17 14594.0 18 11614.0 19 6405.0 20 5100.0 21 5251.0 22 5419.0 23 5860.0 24 6791.0 25 7433.0 26 8435.0 27 9773.0 28 11635.0 29 13447.0 30 15773.0 31 18496.0 32 22038.0 33 26707.0 34 35399.0 35 53134.0 36 53007.0 37 66452.0 38 42035.0 39 2638.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.578013862155 23.44608381729713 17.726499186279938 15.249403134267942 2 15.35581529265831 26.822935478744377 39.22729702563637 18.59395220296095 3 17.419149774925852 33.58609885925281 33.10581684062356 15.88893452519778 4 10.449591027580613 22.294621130344865 38.96125339778372 28.294534444290793 5 8.823450493608782 43.573797763847985 33.25682724002204 14.345924502521193 6 22.189565888624934 43.391529946935755 17.525030598113133 16.893873566326185 7 20.992885551705136 41.303221625280955 22.09008332180959 15.613809501204317 8 25.811185390510357 34.30518031731224 20.187737225674756 19.695897066502653 9 27.678857065016604 17.274260227406415 22.197821703298402 32.849061004278575 10-11 22.48224732690821 31.869363442444808 25.67860566826521 19.969783562381775 12-13 25.998375559497973 31.412902020642985 27.706927815602267 14.881794604256775 14-15 22.262836243851996 30.932989616249095 30.82618001391105 15.977994125987859 16-17 15.255610342059041 34.24749281228135 31.540307983166393 18.956588862493216 18-19 14.660675697151952 29.538892110949895 40.28569246677551 15.51473972512265 20-21 16.682421513001877 27.944487902135574 41.25791784226028 14.115172742602274 22-23 16.304972626560236 28.693143998224986 41.068621936935315 13.933261438279473 24-25 15.680778693261562 29.525865627528358 41.060238924756035 13.73311675445404 26-27 14.679503122255577 29.550128945904643 42.07568335137579 13.694684580463997 28-29 14.246807755299596 29.929441923489325 41.942581391532826 13.881168929678253 30-31 14.671556876031335 30.114828417840457 41.15102924528205 14.062585460846162 32-33 14.013832617485402 31.05868439465271 41.10415329396685 13.823329693895031 34-35 14.523835568939148 30.65311749881839 40.92603409238669 13.89701283985577 36-37 14.618689326149392 30.53911557430086 40.32711635447615 14.515078745073595 38-39 13.862822739520478 31.656465523024035 40.3164470092641 14.164264728191378 40-41 13.914054816602048 31.56923591324368 40.37252338203925 14.144185888115022 42-43 13.98669604751854 31.57557398452861 39.101957404645546 15.335772563307307 44-45 14.34832592372437 31.50105468250135 38.54348552959097 15.607133864183313 46-47 15.035902474061263 32.22801734546663 37.44889134728704 15.287188833185073 48-49 14.898974523561604 32.53454804011917 36.31592145822605 16.250555978093175 50-51 14.818877745832362 32.800764488438766 35.59896967432875 16.781388091400125 52-53 15.120318179097517 33.6488430507712 34.63314255521592 16.597696214915366 54-55 15.486669955232845 33.08455811783937 34.11312942847059 17.315642498457194 56-57 16.08408134454198 33.26721801748994 33.34275872175221 17.305941916215865 58-59 15.952297902816678 32.67723686138694 34.05337796977134 17.31708726602505 60-61 16.37912352143519 33.469175883939755 33.899303828427655 16.252396766197393 62-63 16.573547956995462 33.31386337039506 34.41312509416788 15.699463578441591 64-65 16.763741287535577 33.39115843527545 34.111478265535894 15.73362201165308 66-67 16.689026164740653 33.58197095191608 33.42428489165275 16.304717991690524 68-69 16.412353175495916 33.35844476963181 33.923864877081236 16.30533717779103 70-71 16.806981116887886 33.56834885770484 33.854206440773815 15.77046358463345 72-73 16.2647804882076 33.24141859663534 33.980107614544266 16.51369330061279 74-75 15.406072564483072 34.30074281692524 33.29518458969633 16.99800002889535 76-77 14.861395191400744 33.60880234960486 34.59898412200443 16.930818336989972 78-79 14.530130627627672 33.31582412638001 34.51673556831996 17.637309677672356 80-81 14.897514380597185 33.89414394425674 33.50766861985482 17.700673055291254 82-83 14.976274656710073 33.332610943585486 33.47533451805252 18.21577988165192 84-85 14.48419414493998 33.254937007744005 33.25246024800621 19.00840859930981 86-87 14.338802122569952 33.514066876226764 33.1733081255792 18.973822875624087 88-89 14.631780345794798 32.86495344752501 33.170934578860575 19.332331627819617 90-91 14.755054291903742 32.47699263826348 33.181378614944954 19.58657445488782 92-93 14.969225625085139 32.9527300647686 32.863564873868405 19.21447943627785 94-95 14.597312319533051 32.06331384273086 33.22676452558993 20.11260931214616 96-97 14.663668429971082 32.03699843345917 33.06598253482406 20.23335060174569 98-99 14.77783556859977 32.128188299009715 32.72580731714365 20.36816881524687 100-101 14.63837534406901 31.70006987168174 33.304228733258405 20.357326050990846 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6086.0 1 4014.0 2 2281.0 3 2667.0 4 2236.5 5 2174.5 6 3794.0 7 7587.5 8 10659.0 9 10869.5 10 8432.5 11 5679.5 12 4950.5 13 4643.0 14 4282.5 15 3804.0 16 3374.5 17 3102.5 18 2951.5 19 2896.0 20 2918.0 21 2971.5 22 3049.0 23 3232.0 24 3403.5 25 3865.0 26 4924.5 27 6036.5 28 6221.0 29 6543.0 30 8068.5 31 9505.5 32 11543.0 33 14454.5 34 16690.0 35 18771.0 36 22046.0 37 24238.5 38 23437.0 39 21634.5 40 19415.5 41 16949.0 42 14631.0 43 14142.5 44 14193.0 45 12986.5 46 11312.0 47 11048.0 48 12958.5 49 11375.0 50 7532.5 51 5036.0 52 3265.0 53 2605.0 54 2311.5 55 2203.5 56 2151.5 57 2034.0 58 1958.0 59 1788.0 60 1548.5 61 1526.0 62 1356.5 63 795.0 64 389.5 65 325.0 66 318.0 67 237.0 68 184.0 69 156.5 70 112.0 71 83.0 72 82.0 73 107.5 74 104.0 75 74.0 76 49.0 77 34.0 78 33.5 79 28.0 80 24.0 81 23.0 82 16.0 83 6.5 84 2.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06418895908624643 2 0.0 3 0.0 4 0.0 5 0.002889535135715273 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.159884170920132E-4 12-13 0.0067078494221961705 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.287791507656235E-4 24-25 6.191861005104158E-4 26-27 3.095930502552079E-4 28-29 0.0025799420854600657 30-31 3.095930502552079E-4 32-33 0.0 34-35 0.0 36-37 1.0319768341840262E-4 38-39 5.159884170920132E-4 40-41 1.0319768341840262E-4 42-43 0.013312501160973936 44-45 2.0639536683680524E-4 46-47 0.0 48-49 1.0319768341840262E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.25581467347221E-4 84-85 6.191861005104158E-4 86-87 0.0 88-89 0.0 90-91 0.008771803090564223 92-93 0.002683139768878468 94-95 0.0 96-97 0.0 98-99 2.0639536683680524E-4 100-101 0.00980377992474825 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 484507.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.222782260786722 #Duplication Level Percentage of deduplicated Percentage of total 1 76.32623739386997 22.304650161461794 2 10.12767134180637 5.9191746886083445 3 3.8536878049463166 3.3784643887498604 4 2.0141918094179676 2.354411547123252 5 1.2391332814851772 1.8105461038467736 6 0.8596666886248615 1.507311147512152 7 0.6296506821672082 1.2880101349729707 8 0.4827855681728942 1.1286670029893353 9 0.4267805160615799 1.1224542684612366 >10 3.258202456997409 19.73321962061062 >50 0.47839538512253305 9.667324629553999 >100 0.28519724882304853 14.517324569918957 >500 0.01061528221425739 1.9660988160092812 >1k 0.004953798366653449 3.5746311175681527 >5k 7.076854809504926E-4 1.6678854476822185 >10k+ 0.002123056442851478 8.059826354931074 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15396 3.1776630678194535 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13087 2.70109616579327 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10490 2.165087398118087 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8065 1.6645786335388344 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3761 0.7762529746732245 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3274 0.6757384310237003 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2499 0.5157820217251763 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2153 0.4443692247996417 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2031 0.4191889900455515 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1960 0.40453491900013827 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1607 0.331677354506746 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 961 0.19834594753016985 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 953 0.19669478459547537 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 714 0.14736629192147896 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 655 0.13518896527810745 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 645 0.13312501160973939 No Hit GTACATGGGAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG 638 0.13168024404188175 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 628 0.1296162903735137 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 596 0.12301163863473594 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 558 0.11516861469493733 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 555 0.1145494285944269 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 526 0.10856396295615955 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 524 0.10815117222248594 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 522 0.10773838148881235 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 519 0.10711919538830192 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 513 0.10588082318728109 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 489 0.10092733438319776 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 6.191861005104157E-4 0.0 24-25 0.0 0.0 0.0 0.003715116603062494 0.0 26-27 0.0 0.0 0.0 0.008049419306635405 0.0 28-29 0.0 0.0 0.0 0.0157892455630156 0.0 30-31 0.0 0.0 0.0 0.03446802626174648 0.0 32-33 0.0 0.0 0.0 0.06006105174951033 0.0 34-35 0.0 0.0 0.0 0.08967878689059189 0.0 36-37 0.0 0.0 0.0 0.12662355755438 0.0 38-39 0.0 0.0 0.0 0.17533286412786606 0.0 40-41 0.0 0.0 0.0 0.23776746259599965 0.0 42-43 0.0 0.0 0.0 0.2981381073957652 0.0 44-45 0.0 0.0 0.0 0.3834825915827842 0.0 46-47 0.0 0.0 0.0 0.48059161167950104 0.0 48-49 0.0 0.0 0.0 0.5900843537864262 0.0 50-51 0.0 0.0 0.0 0.7239317491800944 0.0 52-53 0.0 0.0 0.0 0.872226820252339 0.0 54-55 0.0 0.0 0.0 1.0317704388171893 0.0 56-57 0.0 0.0 0.0 1.2283620257292465 0.0 58-59 0.0 0.0 0.0 1.4477603006767703 0.0 60-61 0.0 0.0 0.0 1.698324276016652 0.0 62-63 0.0 0.0 0.0 1.9575568567636794 0.0 64-65 0.0 0.0 0.0 2.2189565888624934 0.0 66-67 0.0 0.0 0.0 2.534122314022295 0.0 68-69 0.0 0.0 0.0 2.8821049025091483 0.0 70-71 0.0 0.0 0.0 3.279003192936325 0.0 72-73 0.0 0.0 0.0 3.6687808432076316 0.0 74-75 0.0 0.0 0.0 4.090962566072316 0.0 76-77 0.0 0.0 0.0 4.520058533726035 0.0 78-79 0.0 0.0 0.0 5.021186484405798 0.0 80-81 0.0 0.0 0.0 5.526751935472553 0.0 82-83 0.0 0.0 0.0 6.059561574961766 0.0 84-85 0.0 0.0 0.0 6.620440984340783 0.0 86-87 0.0 0.0 0.0 7.1906081852274575 0.0 88-89 0.0 0.0 0.0 7.797100970677411 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 50 4.2365355E-7 57.000557 3 ATGGGCG 35 2.7805174E-4 54.308666 7 ACTGTGC 40 5.373862E-4 47.52008 8 CTCGAGT 45 9.599559E-4 42.240074 9 GTATGGT 60 8.252987E-5 39.608246 1 TCTCGAG 50 0.0016115428 38.016068 8 CTGTGCC 65 1.3237535E-4 36.55391 9 GGTATCA 7295 0.0 35.443817 1 TAGATAT 115 1.0304575E-7 33.05745 7 GAGGCGC 90 2.4031484E-5 31.680056 9 ACTGGGC 75 3.0682218E-4 31.680054 8 ACCTGCG 30 0.0039504603 31.666973 94-95 CCACGCG 30 0.0039504603 31.666973 84-85 GTATCAA 10710 0.0 30.577126 1 TGGGGAC 140 1.7031198E-8 30.532862 6 CATGGAT 125 2.1493724E-7 30.4003 4 GTAGATA 125 2.1513188E-7 30.39716 6 GGATATG 95 3.4766705E-5 30.012686 9 GTGGTAC 80 4.4733277E-4 29.706186 1 AGGACAG 80 4.4895886E-4 29.68779 5 >>END_MODULE