FastQCFastQC Report
Thu 26 May 2016
SRR937183_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937183_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279493
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57842.0694614891965095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41811.4959229748151117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30161.0790967931218314No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10160.36351536532220846No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.31736036322913275No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7860.2812235011252518No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.1756752405248074No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4840.17317070552750874No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.16422593768001345No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3970.14204291341822514No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.12701570343443305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2820.10089698131974684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17200.053.6445431
GTATCAA31600.042.744821
TATCAAC41750.032.329752
ATCAACG42000.031.910993
TATAGGG753.0622847E-431.684672
CCTATAC753.0622847E-431.684673
TCAACGC42500.031.5355644
CAACGCA43150.031.0605245
AACGCAG44150.030.346116
CTATAGA700.00832842427.1777941
ATAGGGG700.00835765327.1582893
GGGGTCC700.00837229827.1485466
ACGCAGA49450.026.997577
CGCAGAG49550.026.9430838
GCAGAGT50100.026.362819
GTACATG25550.026.2470471
TACATGG26000.025.4086692
GAGTACT31700.025.01544212-13
ACATGGG25600.024.5061133
ATGGGAT7100.024.09825
Produced by FastQC (version 0.11.5)