##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937183_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 279493 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.926262911772387 33.0 31.0 34.0 31.0 34.0 2 32.003942853667176 33.0 31.0 34.0 30.0 34.0 3 31.986414686593225 33.0 31.0 34.0 30.0 34.0 4 35.654971680864996 37.0 35.0 37.0 35.0 37.0 5 35.68342677634145 37.0 35.0 37.0 35.0 37.0 6 35.782806725034256 37.0 35.0 37.0 35.0 37.0 7 35.73326344488055 37.0 35.0 37.0 35.0 37.0 8 35.72728476205128 37.0 35.0 37.0 35.0 37.0 9 37.34741478319672 39.0 37.0 39.0 35.0 39.0 10-11 37.25402961791529 39.0 37.0 39.0 35.0 39.0 12-13 37.112246102764644 39.0 37.0 39.0 34.0 39.0 14-15 38.121931497389916 40.0 38.0 41.0 33.5 41.0 16-17 38.11414239354832 40.0 38.0 41.0 33.5 41.0 18-19 38.20027871896613 40.0 38.0 41.0 33.5 41.0 20-21 38.1996937311489 40.0 38.0 41.0 34.0 41.0 22-23 38.204704590097066 40.0 38.0 41.0 34.0 41.0 24-25 38.157313421087466 40.0 38.0 41.0 34.0 41.0 26-27 38.03497940914441 40.0 38.0 41.0 34.0 41.0 28-29 37.847175063418405 40.0 37.5 41.0 33.5 41.0 30-31 37.407403763242726 39.5 36.5 41.0 33.0 41.0 32-33 36.96701706303915 39.0 35.0 41.0 33.0 41.0 34-35 36.83283302265173 39.0 35.0 41.0 32.5 41.0 36-37 36.61092406607679 39.0 35.0 41.0 31.5 41.0 38-39 36.39112786366743 39.0 35.0 41.0 31.0 41.0 40-41 36.01568017803666 39.0 35.0 41.0 30.5 41.0 42-43 35.5679838135481 38.5 35.0 40.5 29.5 41.0 44-45 35.01824732640889 38.0 35.0 40.0 25.5 41.0 46-47 34.17942309825291 38.0 34.5 40.0 19.5 41.0 48-49 33.51261927847925 38.0 34.0 40.0 7.0 41.0 50-51 32.4215579638846 37.5 32.0 39.5 2.0 40.5 52-53 32.433790828392844 37.5 33.0 39.5 2.0 40.5 54-55 32.64943486956739 38.0 33.0 40.0 2.0 41.0 56-57 32.37541190655938 38.0 33.0 40.0 2.0 41.0 58-59 32.081184501937436 37.0 33.0 40.0 2.0 41.0 60-61 31.71583188129935 37.0 32.0 40.0 2.0 41.0 62-63 31.3079898244321 36.0 31.0 40.0 2.0 41.0 64-65 30.852776277044505 36.0 31.0 39.0 2.0 41.0 66-67 30.38197021034516 35.0 30.5 39.0 2.0 40.5 68-69 29.855522320773687 35.0 29.0 38.5 2.0 40.0 70-71 29.391737896834627 35.0 29.0 38.0 2.0 40.0 72-73 28.919795844618648 35.0 28.0 37.0 2.0 40.0 74-75 28.393643132386142 34.5 27.0 37.0 2.0 39.0 76-77 27.91261856289782 34.0 26.0 36.0 2.0 39.0 78-79 27.41864733642703 34.0 25.5 36.0 2.0 38.5 80-81 26.89929443671219 34.0 24.5 35.0 2.0 37.0 82-83 26.385106245952493 33.5 23.0 35.0 2.0 37.0 84-85 25.890834475282027 33.0 20.0 35.0 2.0 36.0 86-87 25.341015696278617 33.0 17.5 35.0 2.0 36.0 88-89 24.818424790602986 32.0 9.5 35.0 2.0 35.5 90-91 24.295315088392197 32.0 3.5 35.0 2.0 35.0 92-93 23.668365576239836 31.5 2.0 34.5 2.0 35.0 94-95 22.943617550350098 31.0 2.0 34.0 2.0 35.0 96-97 22.178161885986412 31.0 2.0 34.0 2.0 35.0 98-99 21.123013814299462 30.0 2.0 34.0 2.0 35.0 100-101 18.39483278650986 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09873500245054956 1101 2 0.23053620344537862 1101 3 0.1027093755091606 1101 4 0.09109701102757839 1101 5 0.014361174042463176 1101 6 -0.03840258565568888 1101 7 0.2611201856672807 1101 8 0.09782952264448141 1101 9 0.10312594255434959 1101 10-11 0.05679463853043387 1101 12-13 0.2977438960404797 1101 14-15 0.3280178010058208 1101 16-17 0.4216145274061418 1101 18-19 0.22662892332724027 1101 20-21 0.2818177781560749 1101 22-23 0.37771412387269265 1101 24-25 0.11662268925609709 1101 26-27 0.17378581957851225 1101 28-29 0.37708055861595113 1101 30-31 0.4780108163550949 1101 32-33 0.4445461441656775 1101 34-35 0.42393580416519683 1101 36-37 0.5857102847930236 1101 38-39 0.4775796450426384 1101 40-41 0.7628025669465757 1101 42-43 0.7950911407126924 1101 44-45 0.962707267134995 1101 46-47 1.218680811972412 1101 48-49 1.4193408657295095 1101 50-51 1.4374615577532701 1101 52-53 1.437315608051584 1101 54-55 1.9512175262441218 1101 56-57 1.726292006413395 1101 58-59 1.722768533093447 1101 60-61 2.028632619857394 1101 62-63 2.140363425492186 1101 64-65 2.194451341944923 1101 66-67 2.4902963408656227 1101 68-69 2.634318304096876 1101 70-71 2.5663551196179633 1101 72-73 2.4883993718789235 1101 74-75 2.3872470911559347 1101 76-77 2.5088117039852946 1101 78-79 2.60488780396528 1101 80-81 2.616170805218321 1101 82-83 2.6794479293320244 1101 84-85 2.802234231940961 1101 86-87 2.9117903942713106 1101 88-89 2.7320152066072545 1101 90-91 2.6298190634454883 1101 92-93 2.6899800401315197 1101 94-95 2.5563045025158644 1101 96-97 2.577274318684779 1101 98-99 2.836805854026256 1101 100-101 2.5448467895003937 1102 1 0.15679210754185746 1102 2 0.17991031339116503 1102 3 0.03390474289993506 1102 4 0.2425812870643682 1102 5 0.0462339661179314 1102 6 0.04755796478136887 1102 7 -0.11849727788475661 1102 8 -0.14173176422637823 1102 9 -0.027890037115042787 1102 10-11 -0.03810776038694286 1102 12-13 -0.09822106041079337 1102 14-15 0.06306095634142395 1102 16-17 0.3036002235957227 1102 18-19 0.24712082334219332 1102 20-21 0.13428317914270593 1102 22-23 0.08080777527828786 1102 24-25 0.12961846420319034 1102 26-27 0.3354570365039109 1102 28-29 0.34508778133210427 1102 30-31 0.4736441415582817 1102 32-33 0.08826285318777849 1102 34-35 0.32585478071104035 1102 36-37 0.42379909745086763 1102 38-39 0.5884184399977528 1102 40-41 0.7090590284450897 1102 42-43 0.9118800218211192 1102 44-45 0.9032280422787764 1102 46-47 1.2647334953137417 1102 48-49 1.3288503819334139 1102 50-51 1.4486083803089151 1102 52-53 1.4732915008595597 1102 54-55 1.585864533695002 1102 56-57 1.9245960843369048 1102 58-59 2.2315490899821704 1102 60-61 1.947030876334626 1102 62-63 1.925410908163176 1102 64-65 2.008039568445529 1102 66-67 2.1363045706707346 1102 68-69 2.2367030592877093 1102 70-71 2.199448220271787 1102 72-73 2.002046214104272 1102 74-75 2.1237005644326743 1102 76-77 1.8951444052653983 1102 78-79 1.8697541679807301 1102 80-81 1.9393676832425406 1102 82-83 1.901570054604452 1102 84-85 1.8432967107960287 1102 86-87 1.4164698173981627 1102 88-89 1.8237684855472942 1102 90-91 1.7967224222445317 1102 92-93 2.095689369079821 1102 94-95 1.795818582091627 1102 96-97 1.502039659088716 1102 98-99 1.9142612234626775 1102 100-101 2.0197167727418055 1103 1 0.07896694762714418 1103 2 0.09712228309927795 1103 3 0.05467527447849463 1103 4 -0.061366952035697864 1103 5 0.02255923631864931 1103 6 0.017266074479188376 1103 7 0.1120372416358677 1103 8 0.02983650281280603 1103 9 0.014937696139867285 1103 10-11 -0.04571749496528099 1103 12-13 -0.10114363963290884 1103 14-15 0.13665848593483787 1103 16-17 0.22448839358322914 1103 18-19 0.09840014103941996 1103 20-21 0.13398908312035474 1103 22-23 0.05816238155723141 1103 24-25 0.08579815687284764 1103 26-27 0.18353805995806738 1103 28-29 -0.09166319286097036 1103 30-31 0.27327284533006946 1103 32-33 0.27457268334732277 1103 34-35 0.5741821095839157 1103 36-37 0.39501444926322904 1103 38-39 0.7467523860311331 1103 40-41 1.1890972609280013 1103 42-43 0.9842827863237318 1103 44-45 1.2585327992819373 1103 46-47 1.141176403923403 1103 48-49 1.4846845117768055 1103 50-51 1.3739447526344755 1103 52-53 1.4837319096530308 1103 54-55 2.1628993105496477 1103 56-57 2.137153984491306 1103 58-59 2.123101181740129 1103 60-61 1.934972939418227 1103 62-63 2.440078947277943 1103 64-65 2.299617393605441 1103 66-67 2.357814018505451 1103 68-69 2.8381110439947186 1103 70-71 2.779332052342955 1103 72-73 2.7364407439182585 1103 74-75 2.7263033142304813 1103 76-77 2.663911407691689 1103 78-79 2.585528743379598 1103 80-81 2.6396470744637703 1103 82-83 2.43136565786892 1103 84-85 2.3609976151412972 1103 86-87 2.7622348346763026 1103 88-89 2.987359397980203 1103 90-91 2.4336608826518535 1103 92-93 2.5517036327369027 1103 94-95 2.249204362548948 1103 96-97 2.0441321522877445 1103 98-99 2.220415182730381 1103 100-101 2.438399222561671 1104 1 0.12174222392677692 1104 2 0.11135012619778806 1104 3 0.11213007143531684 1104 4 0.15114733932455948 1104 5 0.13976956279683606 1104 6 0.09532160258905975 1104 7 0.08151371911550598 1104 8 0.05028196980505584 1104 9 0.0787442087656629 1104 10-11 0.037334716137515045 1104 12-13 0.19227386569547633 1104 14-15 0.2368532718820262 1104 16-17 0.2802988019279695 1104 18-19 0.1320677628159217 1104 20-21 -0.030852053413305214 1104 22-23 0.14742515909193088 1104 24-25 0.12966733975991218 1104 26-27 0.14536777751844454 1104 28-29 0.5378377034366864 1104 30-31 0.32123182736177114 1104 32-33 0.5969504833953181 1104 34-35 0.6674888127611851 1104 36-37 0.6979207120548807 1104 38-39 0.5339557961966932 1104 40-41 1.00477752967614 1104 42-43 0.9386668816276895 1104 44-45 0.9379052497738414 1104 46-47 1.0512572824362536 1104 48-49 0.8968332266130048 1104 50-51 0.8977778013168454 1104 52-53 0.6756454698602283 1104 54-55 1.1386745727343524 1104 56-57 1.2747039160339284 1104 58-59 1.5165546801239707 1104 60-61 1.4370266608178603 1104 62-63 1.3924199073750536 1104 64-65 1.5925973362988515 1104 66-67 1.6017587162343645 1104 68-69 1.6947281202294207 1104 70-71 1.4729252884715116 1104 72-73 1.416914158459452 1104 74-75 1.3945108600686993 1104 76-77 1.7283107471132624 1104 78-79 1.8380553147234835 1104 80-81 1.5835261377974348 1104 82-83 2.087868403221581 1104 84-85 2.064877101922672 1104 86-87 2.0564921779653638 1104 88-89 1.7943942759566873 1104 90-91 1.547081352114219 1104 92-93 1.8149787184850652 1104 94-95 1.7181797602369215 1104 96-97 1.510919736800851 1104 98-99 1.5594979949877796 1104 100-101 1.634998937440212 1105 1 0.1707345021065656 1105 2 0.2694191854955399 1105 3 0.2114841581722402 1105 4 0.23929264552629803 1105 5 0.14736511425135035 1105 6 0.32305216448503415 1105 7 0.42494531544399905 1105 8 0.3115514564739925 1105 9 0.31537186162371 1105 10-11 0.4864775455289987 1105 12-13 0.4274737102877708 1105 14-15 0.5211036725425089 1105 16-17 0.3643063780534561 1105 18-19 0.4111120054461992 1105 20-21 0.35261645224451144 1105 22-23 0.3859322738507984 1105 24-25 0.20800739123871637 1105 26-27 0.540559851725618 1105 28-29 0.4503965883864183 1105 30-31 0.6981246446120224 1105 32-33 0.7553692100234528 1105 34-35 0.7798680075550237 1105 36-37 0.8044575485368455 1105 38-39 0.5516868437417415 1105 40-41 0.6682907150483928 1105 42-43 0.9892715147198246 1105 44-45 0.9803412655088763 1105 46-47 1.1836160799691555 1105 48-49 1.2730121397303833 1105 50-51 1.2241242633633753 1105 52-53 1.1777871822588537 1105 54-55 1.3113713820199564 1105 56-57 1.6563658071514027 1105 58-59 1.26960698452881 1105 60-61 1.073513475445612 1105 62-63 1.0654275320654492 1105 64-65 1.432668241063066 1105 66-67 1.677806504102847 1105 68-69 1.7749524900997535 1105 70-71 1.6392552384496604 1105 72-73 1.7177883267854206 1105 74-75 1.3447803953025357 1105 76-77 1.4119200910013667 1105 78-79 1.9880043215711325 1105 80-81 2.1093723813018777 1105 82-83 2.3375065946644433 1105 84-85 2.3965365647948005 1105 86-87 2.1703963108389495 1105 88-89 2.056091133807204 1105 90-91 2.0178889865175975 1105 92-93 1.9543211496443078 1105 94-95 1.979245274296467 1105 96-97 2.0404083483301605 1105 98-99 2.729081793734803 1105 100-101 2.7887457200151253 1106 1 0.01459304991991317 1106 2 -0.5587596524761445 1106 3 -0.177551204874419 1106 4 -0.02380284167465163 1106 5 -0.2967863818775456 1106 6 -0.3380624341060283 1106 7 -0.041553952185211074 1106 8 -0.0951305551044399 1106 9 0.26753758667079097 1106 10-11 -0.46261222220483944 1106 12-13 -0.5350010054826129 1106 14-15 -0.1719632173228831 1106 16-17 -0.22184135159709228 1106 18-19 -0.15571508529802713 1106 20-21 -0.039306544198261406 1106 22-23 -0.07631226824307902 1106 24-25 0.04429113260720641 1106 26-27 -0.06293460318126165 1106 28-29 -0.24270517627842736 1106 30-31 -0.11220527099096245 1106 32-33 -0.08124235446005201 1106 34-35 -0.3476691341064111 1106 36-37 0.040811039504269786 1106 38-39 0.17886128748868657 1106 40-41 0.2648032351014038 1106 42-43 0.5963720238918953 1106 44-45 0.029124135007371876 1106 46-47 -0.4969168069667447 1106 48-49 -0.1880536341414185 1106 50-51 0.10467110021906123 1106 52-53 -0.21774186844910304 1106 54-55 -0.6566135520666663 1106 56-57 -0.8782020940831643 1106 58-59 -0.6991940198654198 1106 60-61 -0.1830813491341452 1106 62-63 -0.02802298059410191 1106 64-65 0.4302758298194327 1106 66-67 0.3416622619322425 1106 68-69 0.33774107906536344 1106 70-71 0.3410199033028398 1106 72-73 -0.10843752241594373 1106 74-75 -0.0889574857046469 1106 76-77 -0.30046046281044525 1106 78-79 -0.07164553544218677 1106 80-81 0.055497791080771464 1106 82-83 -0.025895790771837568 1106 84-85 0.10282797861917103 1106 86-87 0.12971136977735753 1106 88-89 0.35652358132063 1106 90-91 0.44215487198875536 1106 92-93 0.5249495935307564 1106 94-95 0.47923132437667704 1106 96-97 -0.008200147170992267 1106 98-99 -0.20129415660348116 1106 100-101 0.03582669929949489 1107 1 0.1329282701474206 1107 2 0.35521594369255993 1107 3 0.3553077093527648 1107 4 0.14292672146441987 1107 5 0.257097924554067 1107 6 0.16257551582837948 1107 7 0.28153624135622124 1107 8 0.39549787050700047 1107 9 0.4412396730366339 1107 10-11 0.31193087984917867 1107 12-13 0.5121084523497998 1107 14-15 0.8919126430110111 1107 16-17 0.7522273313507668 1107 18-19 0.6147756868909511 1107 20-21 0.37985351833880543 1107 22-23 0.35591229013382275 1107 24-25 0.26077180541975764 1107 26-27 0.3739622645744092 1107 28-29 0.27320763124038905 1107 30-31 0.2514441620422616 1107 32-33 -0.2782700468783261 1107 34-35 0.3431497779643209 1107 36-37 0.3772352851958587 1107 38-39 0.42241439906702993 1107 40-41 0.6671879099266462 1107 42-43 0.3559727211442194 1107 44-45 0.2993183952550069 1107 46-47 0.18828634863913152 1107 48-49 0.25658848493059594 1107 50-51 0.3962950871365969 1107 52-53 -0.06276496043968649 1107 54-55 0.0612418579284828 1107 56-57 0.13584178968415728 1107 58-59 0.11772333416984182 1107 60-61 0.3575857128817752 1107 62-63 0.06066104549895002 1107 64-65 -0.1861904666565657 1107 66-67 0.026640787279518463 1107 68-69 0.04107638914497258 1107 70-71 -0.22899285569585004 1107 72-73 0.002386061964163133 1107 74-75 -0.03994316327936076 1107 76-77 -0.10026997635116075 1107 78-79 -0.17664670351953404 1107 80-81 -0.0186122238346762 1107 82-83 -0.10482187843155444 1107 84-85 0.14750244444841698 1107 86-87 0.32797902122308287 1107 88-89 0.20366718295234776 1107 90-91 0.4986898154717849 1107 92-93 0.8143093074416612 1107 94-95 1.1443255792331826 1107 96-97 1.0400234767440466 1107 98-99 0.9530178458404954 1107 100-101 1.355816995272324 1108 1 -0.07075832709812957 1108 2 -0.16332201398295254 1108 3 -0.013792895282776385 1108 4 0.045681172253807745 1108 5 0.040226913469084025 1108 6 -0.0971238459452195 1108 7 0.04243227763435442 1108 8 0.04854624135013097 1108 9 -0.009171815038612863 1108 10-11 -0.08796834756096672 1108 12-13 -0.17576944486216206 1108 14-15 -0.1944683613558027 1108 16-17 -0.10331096223337255 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-2.450372471043867 2316 60-61 -2.3924341362647112 2316 62-63 -2.4422766281810837 2316 64-65 -2.4261401821521353 2316 66-67 -2.3643609329798423 2316 68-69 -2.784780432345034 2316 70-71 -2.4466718288333382 2316 72-73 -2.115134118001432 2316 74-75 -2.8082242271936124 2316 76-77 -2.68972346318456 2316 78-79 -2.567979242887006 2316 80-81 -3.0647416402697694 2316 82-83 -2.765626430502479 2316 84-85 -2.5802995933972888 2316 86-87 -2.614246729736294 2316 88-89 -2.2877712185297234 2316 90-91 -2.1124654048537437 2316 92-93 -1.7977589736222512 2316 94-95 -1.4692909352566943 2316 96-97 -1.0552259608634706 2316 98-99 -0.772150871904639 2316 100-101 -0.8318500683772747 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1250.0 3 208.0 4 169.0 5 197.0 6 224.0 7 271.0 8 305.0 9 382.0 10 518.0 11 821.0 12 1433.0 13 2095.0 14 2411.0 15 3523.0 16 5092.0 17 7384.0 18 5875.0 19 3264.0 20 3051.0 21 2989.0 22 3103.0 23 3315.0 24 3489.0 25 3666.0 26 3898.0 27 4221.0 28 4770.0 29 5574.0 30 6967.0 31 8512.0 32 10643.0 33 13944.0 34 19410.0 35 27893.0 36 35535.0 37 45286.0 38 33809.0 39 3996.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.51948681315131 19.637940503925364 16.084832341971403 18.757740340951923 2 14.727199661286965 21.798910664437283 42.0836592489469 21.390230425328845 3 17.767508279185247 29.510137741244513 31.88571674602007 20.836637233550164 4 11.092772260175671 17.839715253458866 39.046443538664676 32.02106894770079 5 11.053702328284947 39.61277775983753 33.542157780863505 15.791362131014019 6 26.63668219690041 38.92995613515966 18.381627697615947 16.051733970323983 7 24.259987877179327 37.70089629967039 21.048157725787537 16.99095809736275 8 26.395529427799758 32.99808824215121 20.22912399883788 20.377258331211152 9 28.446748215374683 15.363561358826272 20.835814470710623 35.35387595508843 10-11 23.811138136134367 28.45806696944259 26.32428592952926 21.406508964893778 12-13 26.450907069130952 27.03290199348955 27.922305022732775 18.593885914646723 14-15 22.85504722724852 26.552348091811133 29.56244631229343 21.030158368646916 16-17 18.58147304016942 30.609525824736444 29.518544353848114 21.29045678124602 18-19 18.39743670199509 26.44037588821611 36.425691073820325 18.73649633596847 20-21 20.225146230288946 25.009234653440494 36.743699814589675 18.021919301680885 22-23 19.508840758757827 25.795004850269233 36.73706878775545 17.959085603217495 24-25 18.654595465947992 25.959357421941544 36.9647349517923 18.421312160318166 26-27 18.24097284232194 26.366221629317238 36.9266244221866 18.466181106174222 28-29 18.161977009020642 26.794713150768455 36.89191370312584 18.15139613708506 30-31 18.370637686514293 26.811423938758562 36.58576832499 18.232170049737146 32-33 17.90537510985168 26.977778574887456 36.476137525333144 18.64070878992772 34-35 18.281775971621183 27.105141867184123 36.203436519792895 18.409645641401795 36-37 18.361019660161094 27.01030706001334 36.049605864336996 18.579067415488563 38-39 18.205876183116594 27.518409016339067 35.82313000790687 18.45258479263747 40-41 18.278355376508912 27.55101126093419 35.744546577525824 18.426086785031078 42-43 17.935219187514345 27.937442621069543 35.043643848978654 19.083694342437457 44-45 18.16796220088401 28.1228650582318 34.64141943928921 19.067753301594987 46-47 18.583707380581345 28.453892213421227 33.56687749601445 19.395522909982983 48-49 18.627106363747227 28.718283488403788 32.829862481079495 19.824747666769486 50-51 18.85531125675768 29.129492712290634 32.260535161448864 19.75466086950283 52-53 18.905638646552607 29.404884279566073 31.741538262821102 19.947938811060222 54-55 18.661506428477914 29.08928850047615 31.9576683518742 20.29153671917173 56-57 18.812023200150605 29.23613414492026 31.406083316151356 20.545759338777778 58-59 18.92899721223747 28.542385643471167 31.992757195743955 20.535859948547404 60-61 19.043785845032925 28.47236290815604 32.4124256207163 20.071425626094737 62-63 19.200490527406604 28.199768003485325 32.5123214532233 20.087420015884767 64-65 18.876580548120554 28.18247498641979 32.84223471979949 20.09870974566016 66-67 18.95557949955449 28.197524843890903 32.430533020905976 20.41636263564863 68-69 18.96731627731861 28.064255876077954 32.53903938899547 20.429388457607974 70-71 18.9026664180496 28.014063598158323 32.44685810180869 20.636411881983392 72-73 18.80445014209257 27.993222587788107 32.14322213954656 21.059105130572764 74-75 18.579340788593704 28.09600395892614 32.21988535609716 21.104769896382997 76-77 18.29759458305987 27.779033027411064 32.43977491580861 21.483597473720454 78-79 18.314528970434235 27.68742199715962 32.60213889167826 21.395910140727885 80-81 18.314763480923915 27.89135650171952 32.12560559713402 21.668274420222552 82-83 18.251661613544638 27.697802384977976 31.939018289851383 22.111517711626007 84-85 18.31423490351588 27.306511871383186 31.932443188744596 22.446810036356336 86-87 18.254195903235175 27.422616465222678 31.71532755115578 22.60786008038637 88-89 18.20939622343821 26.91230573942728 31.7438091208342 23.134488916300317 90-91 18.41642418016254 27.109363654455386 31.88702011427793 22.587192051104143 92-93 18.60577698886558 27.34782063005397 31.256880569451166 22.78952181162928 94-95 18.60247884839151 27.443214721770516 30.760597318913725 23.193709110924253 96-97 18.766686330163864 27.52130560839996 30.439810871822086 23.27219718961409 98-99 19.166996896141782 27.783386199934373 29.802956106675175 23.246660797248666 100-101 20.02094049350002 26.639606576892668 29.798609106604683 23.54084382300263 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 720.0 1 493.0 2 344.0 3 426.0 4 367.0 5 345.0 6 605.5 7 1454.0 8 2473.0 9 2712.5 10 2157.5 11 1635.5 12 1490.0 13 1457.5 14 1458.5 15 1477.5 16 1476.0 17 1504.5 18 1558.0 19 1606.5 20 1659.0 21 1785.5 22 1950.0 23 2162.0 24 2430.5 25 2787.5 26 3167.0 27 3594.0 28 3969.0 29 4390.5 30 4960.0 31 5422.5 32 5873.5 33 6152.5 34 6568.0 35 7113.5 36 7628.5 37 8160.0 38 8337.5 39 8118.5 40 7901.5 41 7915.0 42 7913.0 43 7933.0 44 8190.0 45 8444.0 46 8401.0 47 8460.0 48 9153.5 49 9104.5 50 8282.0 51 7678.5 52 7047.5 53 6763.5 54 6448.0 55 5886.5 56 5267.5 57 4665.5 58 4287.0 59 3793.0 60 3176.5 61 2744.0 62 2312.0 63 1665.0 64 1090.5 65 839.0 66 625.5 67 436.5 68 331.5 69 235.0 70 191.5 71 147.5 72 114.0 73 108.0 74 92.0 75 66.0 76 44.0 77 32.5 78 21.5 79 13.0 80 10.5 81 8.5 82 7.5 83 4.0 84 2.0 85 1.5 86 0.5 87 1.5 88 1.5 89 1.5 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32916745678782655 2 0.28301245469475084 3 0.27979233826965255 4 0.28229687326695124 5 0.28265466398085104 6 0.2440132668796714 7 0.24329768545187178 8 0.24759117401866954 9 0.2586826861495637 10-11 0.26440733757196067 12-13 0.25421030222581603 14-15 0.2438343715227215 16-17 0.23667855724472525 18-19 0.22666041725553054 20-21 0.23327954546267707 22-23 0.22988053368062888 24-25 0.2316694872501279 26-27 0.2289860568958793 28-29 0.2461600111630703 30-31 0.2602927443621128 32-33 0.2533158254410665 34-35 0.24812786008951926 36-37 0.23435291760437651 38-39 0.22236692868873284 40-41 0.21771564940803526 42-43 0.23077501046537838 44-45 0.23274285939182734 46-47 0.24097204581152298 48-49 0.24866454616036898 50-51 0.2633339654302612 52-53 0.2799712336266024 54-55 0.24848565080341903 56-57 0.22022018440533395 58-59 0.21306437012733773 60-61 0.21521111441073656 62-63 0.21807344012193508 64-65 0.2127065794134379 66-67 0.21521111441073656 68-69 0.21753675405108536 70-71 0.22075687047618367 72-73 0.22379809154433206 74-75 0.2252292543999313 76-77 0.2375730340294748 78-79 0.23506849903217614 80-81 0.2275548940402801 82-83 0.2227247194026326 84-85 0.2100231490591893 86-87 0.21109652120088876 88-89 0.22004128904838405 90-91 0.2187890215497347 92-93 0.22469256832908158 94-95 0.21950460297753432 96-97 0.22701820796943037 98-99 0.2313116965362281 100-101 0.21735785869413546 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 279493.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.20244842756685 #Duplication Level Percentage of deduplicated Percentage of total 1 81.75952697393667 51.67402287032493 2 10.859294768779856 13.726680351671106 3 3.3424197026833995 6.337473266463927 4 1.428005256446292 3.6101371429936453 5 0.741800999420237 2.344181970468754 6 0.4568964083910093 1.732618301284397 7 0.2904023389852382 1.2847897197071512 8 0.1947813871229995 0.9848528459433048 9 0.1565615283568849 0.8905564729545355 >10 0.7093759377423071 7.8105236082627165 >50 0.03893050158631343 1.6959641097481097 >100 0.018618935541280333 2.320361325090612 >500 0.0011284203358351717 0.5965828333912084 >1k 0.0016926305037527575 2.92871178161506 >5k 5.642101679175859E-4 2.062543400080544 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5784 2.0694614891965095 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4181 1.4959229748151117 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3016 1.0790967931218314 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1016 0.36351536532220846 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 887 0.31736036322913275 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 786 0.2812235011252518 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 491 0.1756752405248074 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 484 0.17317070552750874 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 459 0.16422593768001345 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 397 0.14204291341822514 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 355 0.12701570343443305 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 282 0.10089698131974684 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.788953569499057E-4 0.0 22-23 0.0 0.0 0.0 7.155814277996228E-4 0.0 24-25 0.0 0.0 0.0 8.944767847495285E-4 0.0 26-27 0.0 0.0 0.0 0.0017889535694990573 0.0 28-29 0.0 0.0 0.0 0.005009069994597361 0.0 30-31 0.0 0.0 0.0 0.015563896054641799 0.0 32-33 0.0 0.0 0.0 0.024508663902137084 0.0 34-35 0.0 0.0 0.0 0.03291674567878265 0.0 36-37 0.0 0.0 0.0 0.03953587388592916 0.0 38-39 0.0 0.0 0.0 0.04848064173342445 0.0 40-41 0.0 0.0 0.0 0.06493901457281578 0.0 42-43 0.0 0.0 0.0 0.10411709774484512 0.0 44-45 0.0 0.0 0.0 0.15599675126031778 0.0 46-47 0.0 0.0 0.0 0.21091762584393886 0.0 48-49 0.0 0.0 0.0 0.33006193357257607 0.0 50-51 0.0 0.0 0.0 0.4846275219772946 0.0 52-53 0.0 0.0 0.0 0.6272071214663695 0.0 54-55 0.0 0.0 0.0 0.8211296884000672 0.0 56-57 0.0 0.0 0.0 1.0445699892304994 0.0 58-59 0.0 0.0 0.0 1.3331281999906974 0.0 60-61 0.0 0.0 0.0 1.6699881571273698 0.0 62-63 0.0 0.0 0.0 2.091286722744398 0.0 64-65 0.0 0.0 0.0 2.476806216971445 0.0 66-67 0.0 0.0 0.0 2.9320949004089547 0.0 68-69 0.0 0.0 0.0 3.5301420786924895 0.0 70-71 0.0 0.0 0.0 4.178816642992848 0.0 72-73 0.0 0.0 0.0 4.9053106875664145 0.0 74-75 0.0 0.0 0.0 5.682968804227655 0.0 76-77 0.0 0.0 0.0 6.44971430411495 0.0 78-79 0.0 0.0 0.0 7.265477131806521 0.0 80-81 0.0 0.0 0.0 8.212728046856272 0.0 82-83 0.0 0.0 0.0 9.33529641171693 0.0 84-85 0.0 0.0 0.0 10.421012333045908 0.0 86-87 0.0 0.0 0.0 11.46003656621096 0.0 88-89 0.0 0.0 0.0 12.800857266550505 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1720 0.0 53.644543 1 GTATCAA 3160 0.0 42.74482 1 TATCAAC 4175 0.0 32.32975 2 ATCAACG 4200 0.0 31.91099 3 TATAGGG 75 3.0622847E-4 31.68467 2 CCTATAC 75 3.0622847E-4 31.68467 3 TCAACGC 4250 0.0 31.535564 4 CAACGCA 4315 0.0 31.060524 5 AACGCAG 4415 0.0 30.34611 6 CTATAGA 70 0.008328424 27.177794 1 ATAGGGG 70 0.008357653 27.158289 3 GGGGTCC 70 0.008372298 27.148546 6 ACGCAGA 4945 0.0 26.99757 7 CGCAGAG 4955 0.0 26.943083 8 GCAGAGT 5010 0.0 26.36281 9 GTACATG 2555 0.0 26.247047 1 TACATGG 2600 0.0 25.408669 2 GAGTACT 3170 0.0 25.015442 12-13 ACATGGG 2560 0.0 24.506113 3 ATGGGAT 710 0.0 24.0982 5 >>END_MODULE