FastQCFastQC Report
Thu 26 May 2016
SRR937183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937183_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279493
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52231.868740898698715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42441.5184637897907998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30181.0798123745496309No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17520.6268493307524696No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8990.3216538517959305No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8000.2862325711198491No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5220.18676675265570158No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4450.1592168676854161No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.13631826199582817No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.13274035485683003No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA2820.10089698131974684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19700.053.059381
TCGAGAA405.37904E-447.5037549
ATCGAGA405.37904E-447.5037548
GATCGAG459.608808E-442.2255637
GTATCAA34150.040.0689051
TTCGATA250.00161589837.9962116-17
ACAGTAC1051.8936989E-631.6691728
AGATCGA600.003947810331.6635046
CGATAAG300.003951161631.66350418-19
GAGATCG600.003947810331.6635045
GTTATTC804.48766E-429.6845363
TATCAAC46250.029.5754262
GTATAGA650.00582924529.2383161
ATCAACG46750.029.1575163
TCAACGC47750.028.546894
CAACGCA48150.028.4083775
AACGCAG49150.027.8303876
GCACCGC350.00837739127.14986488-89
CCGTATC350.00838836927.14257692-93
TAAGTCG350.00839203127.14014622-23