##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937183_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 279493 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0957090159682 31.0 31.0 34.0 31.0 34.0 2 32.27376714264758 33.0 31.0 34.0 31.0 34.0 3 32.27301578214839 34.0 31.0 34.0 30.0 34.0 4 36.009735485325216 37.0 35.0 37.0 35.0 37.0 5 35.9570722701463 37.0 35.0 37.0 35.0 37.0 6 36.03518871671205 37.0 35.0 37.0 35.0 37.0 7 35.971730955694774 37.0 35.0 37.0 35.0 37.0 8 35.966389140336254 37.0 35.0 37.0 35.0 37.0 9 37.59764645268397 39.0 37.0 39.0 35.0 39.0 10-11 37.517966460698474 39.0 37.0 39.0 35.0 39.0 12-13 37.39025485432551 39.0 37.0 39.0 34.0 39.0 14-15 38.40071486584637 40.0 38.0 41.0 34.0 41.0 16-17 38.398670091916436 40.0 38.0 41.0 34.0 41.0 18-19 38.539412436089634 40.0 38.0 41.0 34.0 41.0 20-21 38.535213762062014 40.0 38.0 41.0 34.0 41.0 22-23 38.44875005814099 40.0 38.0 41.0 34.0 41.0 24-25 38.42547398324824 40.0 38.0 41.0 34.0 41.0 26-27 38.21972822217372 40.0 37.5 41.0 34.0 41.0 28-29 38.00042040408883 40.0 37.0 41.0 33.0 41.0 30-31 37.78631486298404 39.5 36.5 41.0 33.0 41.0 32-33 37.4442740963101 39.0 35.0 41.0 33.0 41.0 34-35 37.19172930985749 39.0 35.0 41.0 33.0 41.0 36-37 36.91208903264125 39.0 35.0 41.0 32.5 41.0 38-39 36.67113666531899 39.0 35.0 41.0 32.0 41.0 40-41 36.31819580454609 39.0 35.0 41.0 31.0 41.0 42-43 35.89980607743307 39.0 35.0 41.0 30.5 41.0 44-45 35.225064670671536 38.0 35.0 40.0 27.5 41.0 46-47 34.387938159453014 38.0 34.5 40.0 22.0 41.0 48-49 33.691258815068714 38.0 34.0 40.0 11.5 41.0 50-51 33.27874043357079 38.0 33.0 40.0 4.0 41.0 52-53 32.93748501751385 38.0 33.0 40.0 2.0 41.0 54-55 32.66156218581503 38.0 33.0 40.0 2.0 41.0 56-57 32.28990887070517 37.0 33.0 40.0 2.0 41.0 58-59 31.967494713642203 37.0 32.0 40.0 2.0 41.0 60-61 31.576751474992218 36.0 31.5 39.5 2.0 41.0 62-63 31.140100109841747 36.0 31.0 39.0 2.0 41.0 64-65 30.65794313274393 35.0 30.5 39.0 2.0 40.0 66-67 30.171113408922587 35.0 29.5 38.5 2.0 40.0 68-69 29.679562636631328 35.0 29.0 38.0 2.0 40.0 70-71 29.210187732787585 35.0 28.5 37.5 2.0 40.0 72-73 28.715726333038752 34.0 28.0 37.0 2.0 39.0 74-75 28.22894669991735 34.0 26.5 36.0 2.0 39.0 76-77 26.986352073218292 32.5 25.5 35.0 2.0 37.5 78-79 27.556214288014367 34.0 26.0 35.0 2.0 37.5 80-81 27.339852876458444 34.0 26.0 35.0 2.0 37.0 82-83 26.930867678260277 34.0 25.5 35.0 2.0 37.0 84-85 26.501899868690806 33.0 24.5 35.0 2.0 36.0 86-87 26.114471561005104 33.0 23.5 35.0 2.0 36.0 88-89 25.660800091594425 33.0 20.5 35.0 2.0 35.0 90-91 25.07239716200406 32.0 17.5 35.0 2.0 35.0 92-93 24.504556464741512 32.0 9.0 34.5 2.0 35.0 94-95 23.984668667909393 31.5 2.0 34.0 2.0 35.0 96-97 23.318753958059773 31.0 2.0 34.0 2.0 35.0 98-99 22.374857330952832 31.0 2.0 34.0 2.0 35.0 100-101 19.868805301027216 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20774769889761302 1101 2 0.260407094385549 1101 3 0.28043784245188164 1101 4 0.2319204568782851 1101 5 0.22461165969942698 1101 6 0.1590765318730618 1101 7 0.23083274344148919 1101 8 0.15435926679636225 1101 9 0.22221784710172443 1101 10-11 0.22088184580265136 1101 12-13 0.18315829854788745 1101 14-15 0.2938850620730378 1101 16-17 0.26562210575038847 1101 18-19 0.2174094928088124 1101 20-21 0.3007478977223954 1101 22-23 0.4177235092339018 1101 24-25 0.2947420667498122 1101 26-27 0.24598421717294627 1101 28-29 0.3402777345526786 1101 30-31 0.5328833220478302 1101 32-33 0.7936320664743803 1101 34-35 0.7069162109709382 1101 36-37 1.066969326984463 1101 38-39 1.0555680256329438 1101 40-41 1.0766688756848097 1101 42-43 1.107498236444215 1101 44-45 1.5239983738790528 1101 46-47 1.6825213433757327 1101 48-49 1.5673096483350761 1101 50-51 1.62467259660297 1101 52-53 1.7230472855202166 1101 54-55 1.6382044748123974 1101 56-57 1.5611544410809621 1101 58-59 1.7885232298985407 1101 60-61 1.8152855310805904 1101 62-63 1.7981765742932971 1101 64-65 2.049909200391653 1101 66-67 2.1762703093726685 1101 68-69 2.205266165141829 1101 70-71 2.1731692065687653 1101 72-73 2.152951739079956 1101 74-75 1.8588988855475463 1101 76-77 2.046414641664146 1101 78-79 2.1301583743950516 1101 80-81 1.9679935954585268 1101 82-83 1.9155921687124788 1101 84-85 2.1826160386449907 1101 86-87 2.256228241946925 1101 88-89 2.7571235729185872 1101 90-91 2.867670852054342 1101 92-93 2.4913509299256873 1101 94-95 2.611138646532485 1101 96-97 2.6318598633069534 1101 98-99 3.0565165862158246 1101 100-101 3.0523900720832735 1102 1 0.18537930890241228 1102 2 0.2640931973446641 1102 3 0.2035528944979248 1102 4 0.10724205657616181 1102 5 0.11510253688965122 1102 6 0.1469521249147121 1102 7 0.09594576557384471 1102 8 0.1105745005904879 1102 9 0.20206426603499494 1102 10-11 0.1514969568578195 1102 12-13 0.2739634976446581 1102 14-15 0.22189393704451277 1102 16-17 0.2699376393108466 1102 18-19 0.3722572886215261 1102 20-21 0.4017026652658089 1102 22-23 0.4098969331003417 1102 24-25 0.08733878269564599 1102 26-27 0.056024028658484326 1102 28-29 0.06575747868914306 1102 30-31 -0.06477283575041781 1102 32-33 0.23590541244877983 1102 34-35 0.3334317618407354 1102 36-37 0.376653116786116 1102 38-39 0.5196295052669271 1102 40-41 0.7691920269291188 1102 42-43 0.566288234791962 1102 44-45 0.2563172748932203 1102 46-47 0.4998389461892785 1102 48-49 0.2990856654555927 1102 50-51 0.3725344995290314 1102 52-53 0.3467481014196281 1102 54-55 0.2839747011713811 1102 56-57 0.5176332489394007 1102 58-59 0.8044084846999162 1102 60-61 0.928337167993547 1102 62-63 1.0938358635087901 1102 64-65 1.5221257402542783 1102 66-67 1.4059160973017626 1102 68-69 1.397515907468673 1102 70-71 1.56475765337008 1102 72-73 1.4929406453656107 1102 74-75 1.151851623177265 1102 76-77 1.2714813219684764 1102 78-79 1.3630044235378485 1102 80-81 1.3545524983609916 1102 82-83 1.2600205277082246 1102 84-85 0.8129691492123463 1102 86-87 1.0579949749660678 1102 88-89 1.2265627502527927 1102 90-91 1.6039650007101152 1102 92-93 1.2952872315488335 1102 94-95 1.4110406913951472 1102 96-97 1.1493167276410574 1102 98-99 1.6092372999901308 1102 100-101 2.2580840586606676 1103 1 0.07873273879727094 1103 2 -0.035223029403368855 1103 3 -0.09091074788130982 1103 4 0.0036834947057116096 1103 5 0.0025005223318999015 1103 6 0.006558036244754817 1103 7 0.10175416201528265 1103 8 0.07072448956188992 1103 9 0.23606911861936197 1103 10-11 0.03174473275664269 1103 12-13 0.0055457342449045655 1103 14-15 -0.06801121698827473 1103 16-17 0.10875463806092966 1103 18-19 0.1265400619470114 1103 20-21 0.06988882804795793 1103 22-23 0.23799780803599901 1103 24-25 0.07851590202511005 1103 26-27 0.0946608935948845 1103 28-29 0.12476049317336901 1103 30-31 -0.10236198360719584 1103 32-33 -0.16070145469333852 1103 34-35 -0.013858878553357101 1103 36-37 0.15106308664127255 1103 38-39 0.1076193589541603 1103 40-41 0.025717282425034682 1103 42-43 -0.23365294600356634 1103 44-45 0.1334770425573666 1103 46-47 0.192214506809826 1103 48-49 -0.11092095170491234 1103 50-51 -0.11371651142604122 1103 52-53 -0.04923381167498775 1103 54-55 0.09759134700624372 1103 56-57 0.19576353347730446 1103 58-59 -0.08376150280100347 1103 60-61 0.2340683642291168 1103 62-63 0.4807743239161155 1103 64-65 0.41285068694937266 1103 66-67 0.6082909226822473 1103 68-69 0.71609542368072 1103 70-71 0.5414321625973457 1103 72-73 0.3848787381529064 1103 74-75 0.7446940612332895 1103 76-77 0.5685366855446823 1103 78-79 0.21060754095568512 1103 80-81 0.6960715043164356 1103 82-83 0.9684978455524984 1103 84-85 1.2368350414261506 1103 86-87 0.6272178262270067 1103 88-89 0.8406720069250966 1103 90-91 1.4820438184441045 1103 92-93 1.518689922527436 1103 94-95 1.8506774828075443 1103 96-97 1.8884719368168525 1103 98-99 1.9412165662205503 1103 100-101 1.9824793237147063 1104 1 0.23150561555987537 1104 2 0.1444458344864188 1104 3 0.25675393799106416 1104 4 0.1892735690913554 1104 5 0.15335376947009394 1104 6 0.12443882159911368 1104 7 0.2088299732705181 1104 8 0.13456628949695926 1104 9 0.41194812549333193 1104 10-11 0.27228067640987064 1104 12-13 0.05427397961874192 1104 14-15 0.30695878879884475 1104 16-17 0.16349584093472913 1104 18-19 0.20396041848998436 1104 20-21 0.29627808941400247 1104 22-23 0.31702726588178365 1104 24-25 0.08313853953975325 1104 26-27 0.25017912301885303 1104 28-29 0.11606570016872553 1104 30-31 0.36783082625066754 1104 32-33 0.39869767300437786 1104 34-35 0.40608833163197033 1104 36-37 0.288567144681835 1104 38-39 0.29203436594104204 1104 40-41 0.32758609832408325 1104 42-43 0.42240165052133705 1104 44-45 0.30067184261947233 1104 46-47 0.833878938613573 1104 48-49 1.0269465656211523 1104 50-51 1.1579463677360948 1104 52-53 1.0853555146196996 1104 54-55 1.0780986373643415 1104 56-57 1.4047032204082974 1104 58-59 1.4762763251945366 1104 60-61 1.5107596849625295 1104 62-63 1.4686319607955873 1104 64-65 1.473943605492149 1104 66-67 1.349028003998324 1104 68-69 1.6275242774077654 1104 70-71 1.31843401165235 1104 72-73 1.3575981193546518 1104 74-75 1.8081159421880244 1104 76-77 1.7244324891778646 1104 78-79 1.952999597076623 1104 80-81 1.9342043822082324 1104 82-83 1.910337302410234 1104 84-85 2.0013416542679074 1104 86-87 2.0563820815942044 1104 88-89 2.039720661067122 1104 90-91 1.827051369928288 1104 92-93 1.9081347825779673 1104 94-95 1.9601881184025594 1104 96-97 2.034346144616759 1104 98-99 1.945892134699104 1104 100-101 2.6093160281623184 1105 1 0.1272173342719327 1105 2 0.25952523810936157 1105 3 0.2019338709714873 1105 4 0.17219814779078746 1105 5 -0.039788865661755324 1105 6 0.09901023629485195 1105 7 0.018912047963347334 1105 8 0.10647634728920252 1105 9 0.33600232392954155 1105 10-11 0.1688617069571947 1105 12-13 0.2596452583327391 1105 14-15 0.17061281240138726 1105 16-17 0.30810756450328114 1105 18-19 0.39491277631622523 1105 20-21 0.22526162591052667 1105 22-23 0.22872169548695354 1105 24-25 0.05428612829211232 1105 26-27 -0.08540924082943491 1105 28-29 0.08688790404420388 1105 30-31 0.38935964952296587 1105 32-33 0.6090614241577867 1105 34-35 0.5440855148673691 1105 36-37 0.3608025873509604 1105 38-39 0.2941226569419655 1105 40-41 0.6527524331305514 1105 42-43 0.12819058743552603 1105 44-45 0.5103702545470128 1105 46-47 0.9464183976005103 1105 48-49 0.551573829959942 1105 50-51 0.7712045873466096 1105 52-53 0.3534338012497784 1105 54-55 0.8287761178169717 1105 56-57 0.8060290423693495 1105 58-59 1.1464380463348398 1105 60-61 0.9543029805771184 1105 62-63 1.2830411687019527 1105 64-65 0.7693882172157167 1105 66-67 1.0560407765688637 1105 68-69 1.1602133163082549 1105 70-71 1.37246247068785 1105 72-73 0.8189064579491792 1105 74-75 0.4660975846531805 1105 76-77 0.889247214057665 1105 78-79 1.134248686484618 1105 80-81 1.2763587214826693 1105 82-83 1.2070300322436545 1105 84-85 1.3017010345796969 1105 86-87 1.2270925861835913 1105 88-89 1.6624053291259813 1105 90-91 1.9264831605164119 1105 92-93 2.1836143610345786 1105 94-95 1.9776374894870123 1105 96-97 1.5755680376768346 1105 98-99 1.9640311274629347 1105 100-101 2.782629611766808 1106 1 -0.14733103707493456 1106 2 0.14951617066149936 1106 3 0.00890883086547234 1106 4 0.2784407368959023 1106 5 0.05004861777271685 1106 6 0.11520609316868047 1106 7 0.08379312489715574 1106 8 -0.09058021963554097 1106 9 -0.42213396678678805 1106 10-11 -0.43743668182707296 1106 12-13 -0.7343283763188566 1106 14-15 -0.22082193684880735 1106 16-17 0.008261013620945334 1106 18-19 0.24824331966559754 1106 20-21 -0.03489740317505152 1106 22-23 0.09264720291528761 1106 24-25 0.18623144460655539 1106 26-27 0.4075599995974386 1106 28-29 0.2857509855814868 1106 30-31 0.4035118431478608 1106 32-33 0.6363195674234632 1106 34-35 0.42690618864849483 1106 36-37 1.1408611770140737 1106 38-39 1.0193092371191312 1106 40-41 0.8814613680541825 1106 42-43 1.0039456268440432 1106 44-45 1.0594181725214895 1106 46-47 1.7262816883865852 1106 48-49 1.784384041301223 1106 50-51 1.7933361350902075 1106 52-53 1.7381377142022814 1106 54-55 1.7323193974431064 1106 56-57 1.6165961066087284 1106 58-59 1.57755334242189 1106 60-61 1.7008678128293298 1106 62-63 1.8724252797433145 1106 64-65 1.895948864491995 1106 66-67 1.9547504089884775 1106 68-69 2.1721962974061135 1106 70-71 2.250644796139479 1106 72-73 2.2839898087199124 1106 74-75 2.078812936539908 1106 76-77 1.890928179392116 1106 78-79 2.459756114816905 1106 80-81 2.0660567057192587 1106 82-83 2.2607093235877294 1106 84-85 2.439707591041998 1106 86-87 2.396430662489653 1106 88-89 2.274848657576843 1106 90-91 2.032830374019934 1106 92-93 1.9999078673469128 1106 94-95 1.4255624389185542 1106 96-97 2.5601812761507112 1106 98-99 2.3005031927485575 1106 100-101 1.943374537483482 1107 1 0.10326647941293032 1107 2 0.21381433588154408 1107 3 -0.15393912140509514 1107 4 0.021032430967458993 1107 5 0.03955389209118465 1107 6 0.08269760214490418 1107 7 0.09504260824756017 1107 8 0.17933549909143665 1107 9 0.317780134602323 1107 10-11 0.22489012824355115 1107 12-13 0.24844697545212568 1107 14-15 0.3545370223044273 1107 16-17 0.2751936425137913 1107 18-19 0.24574543119003067 1107 20-21 -0.004787963313063415 1107 22-23 0.20324615703680138 1107 24-25 0.11820441709383545 1107 26-27 -0.5355905745575029 1107 28-29 -0.21758252745605233 1107 30-31 0.01801037855857146 1107 32-33 0.12729841623831817 1107 34-35 -0.0018770341667604384 1107 36-37 -0.16257836150552407 1107 38-39 -0.1282369019359848 1107 40-41 0.2091879301329982 1107 42-43 0.046930873128658845 1107 44-45 -0.14560635710274994 1107 46-47 0.17084294056549965 1107 48-49 0.209770854606532 1107 50-51 -0.10686513362149697 1107 52-53 0.18975235650105304 1107 54-55 -0.08371740597846156 1107 56-57 0.18433560739044097 1107 58-59 0.4461715967752191 1107 60-61 0.38698745989027117 1107 62-63 0.36504842205107835 1107 64-65 0.34251381857757224 1107 66-67 0.37007378670875113 1107 68-69 0.29291259837471983 1107 70-71 0.11744803800336712 1107 72-73 -0.4916405037856997 1107 74-75 -0.07272263590784434 1107 76-77 0.1302582421088836 1107 78-79 0.13076263807961297 1107 80-81 0.28510714869013043 1107 82-83 -0.18573882276337628 1107 84-85 -0.44255919782353104 1107 86-87 0.168799343640476 1107 88-89 0.1592449412938457 1107 90-91 0.7249880824318851 1107 92-93 1.1283334787904806 1107 94-95 0.9429398357234113 1107 96-97 0.9364369592962554 1107 98-99 1.1965083682297148 1107 100-101 1.8177343412464886 1108 1 0.006356838835166911 1108 2 0.05725991868302316 1108 3 0.1087624237746212 1108 4 0.007449125626514785 1108 5 0.10770135598100694 1108 6 0.114671905134486 1108 7 0.05706393844575075 1108 8 -0.006317003427881218 1108 9 0.12883152862315228 1108 10-11 -0.07237851468556755 1108 12-13 0.05600282026233572 1108 14-15 0.06712519832055364 1108 16-17 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2316 46-47 -0.6110546378386275 2316 48-49 -0.7821703919198768 2316 50-51 -0.9912504717186224 2316 52-53 -0.46500482227359186 2316 54-55 -0.3187842314383005 2316 56-57 -0.42627706959778067 2316 58-59 -0.5223344241325449 2316 60-61 -0.1833446824942726 2316 62-63 0.17425842602090924 2316 64-65 0.17324029733898527 2316 66-67 -0.4411343646740775 2316 68-69 -0.7835463138920531 2316 70-71 -0.06847230075539557 2316 72-73 0.2903497039686975 2316 74-75 -0.11587468036993442 2316 76-77 -0.3697152923624678 2316 78-79 -0.5218376001101461 2316 80-81 -0.3812683971613957 2316 82-83 -0.21957500114103823 2316 84-85 -0.19624452268789483 2316 86-87 0.4293524731892404 2316 88-89 0.3173103111496083 2316 90-91 0.29530699205210453 2316 92-93 0.10117984639667199 2316 94-95 0.9798460154243536 2316 96-97 0.7825531429670249 2316 98-99 0.2149813072266724 2316 100-101 0.9017359291782086 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 0.0 5 12.0 6 28.0 7 54.0 8 154.0 9 228.0 10 460.0 11 792.0 12 1281.0 13 1859.0 14 2445.0 15 3685.0 16 5418.0 17 7623.0 18 6421.0 19 3400.0 20 2811.0 21 2658.0 22 2771.0 23 2888.0 24 3065.0 25 3400.0 26 3708.0 27 4206.0 28 4938.0 29 5868.0 30 6991.0 31 8876.0 32 10862.0 33 14017.0 34 19080.0 35 27679.0 36 37500.0 37 49270.0 38 32724.0 39 2319.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.10861589453753 20.843012538522938 16.422494174580052 17.62587739235948 2 15.817211880082866 23.204158959258372 40.08544042247927 20.89318873817949 3 18.63266700776048 29.68339099727006 31.888455167034596 19.79548682793487 4 11.824267512960969 19.27991040920524 37.69146275577563 31.204359322058156 5 11.399968514304524 40.9543028065204 32.36693715741416 15.27879152176091 6 27.1402146028702 39.42066527605343 17.212953455006026 16.22616666607035 7 24.550167624949463 38.58844407552247 20.13216788971459 16.729220409813482 8 27.280826353432825 34.11248224463582 19.040548421606264 19.56614298032509 9 28.59892734343973 16.00111630702737 20.195139055360958 35.204817294171946 10-11 24.51617750712898 29.472115580712217 25.129788581467157 20.881918330691647 12-13 26.92741208295762 27.92019122046173 27.085390813975124 18.06700588260552 14-15 23.346559663390494 27.548274912072934 28.8397204938943 20.26544493064227 16-17 18.793136142944547 31.42404282039264 28.95367683627139 20.829144200391422 18-19 18.737678582290076 27.052198087250844 35.856890870254354 18.353232460204726 20-21 20.517150697870786 25.760215819358624 36.379086417191125 17.34354706557946 22-23 19.812552774319123 26.38393943297125 36.251663732772315 17.551844059937316 24-25 19.10727638974858 26.78367615646903 36.49751514349197 17.611532310290418 26-27 18.703989609590376 27.17392665530067 36.420818745672904 17.70126498943606 28-29 18.356149189220133 27.59907543902334 36.30243591761961 17.74233945413691 30-31 18.571842586397512 27.396034963308562 36.2345389687756 17.79758348151832 32-33 18.393841706232354 27.694253523344052 35.81807057779622 18.093834192627366 34-35 18.755568117985067 27.844704518538926 35.66082155903726 17.73890580443875 36-37 18.790631607947248 27.942918069504426 35.34113555616777 17.925314766380556 38-39 18.809952306497838 28.13666174108117 35.26224270375287 17.791143248668124 40-41 18.76469178118951 28.15669802105956 35.25508688947487 17.82352330827606 42-43 18.489971910614926 28.751722040327053 34.4798096362693 18.278496412788723 44-45 18.77954009581635 28.986772477307127 33.8711524081104 18.362535018766124 46-47 19.250571570665457 29.362810517615827 32.61029793232746 18.776319979391253 48-49 19.24126901210406 29.708794137956946 31.88255162025525 19.16738522968375 50-51 19.398160240149124 30.172848693885 31.203822635987304 19.225168429978567 52-53 19.48778681398103 30.476613009985936 30.66337976264164 19.372220413391393 54-55 19.028741328047573 30.501300569245025 30.70381011331232 19.766147989395083 56-57 19.516231175736067 30.312208176948975 30.303084513744533 19.86847613357043 58-59 19.322487504159316 29.800567456072248 30.79701459428322 20.079930445485218 60-61 19.343597156279404 29.861749668149113 31.218134264543295 19.576518911028185 62-63 19.66238868236414 29.610401691634497 31.454276135717173 19.272933490284196 64-65 19.50764419860247 29.552976282053578 31.452844972861577 19.486534546482382 66-67 19.481167685773883 29.666574833716762 30.951043496617086 19.901213983892262 68-69 19.417480938699715 29.47139999928442 31.08235268861832 20.028766373397545 70-71 19.498878326111925 29.61791529662639 30.74728168505115 20.135924692210537 72-73 19.351826342699102 29.513619303524596 30.640660052309006 20.4938943014673 74-75 19.12641819294222 29.685179950839558 30.231526370964566 20.956875485253658 76-77 18.867198820721807 29.191428765657818 31.02188605796925 20.919486355651127 78-79 18.703867359826543 29.248854175238737 30.782166279656376 21.265112185278344 80-81 18.548943980707925 29.703963963319296 30.402192541494777 21.344899514478 82-83 18.35476213051202 29.408049976475137 30.276233803174694 21.960954089838154 84-85 18.336091308353534 29.302396307593227 30.1070690626761 22.25444332137714 86-87 18.097233204409413 29.150461728916287 29.90003327453639 22.852271792137905 88-89 18.127824310447846 28.648839148028753 30.055135549011965 23.16820099251144 90-91 18.299933265465683 29.01288352229524 29.888662464597598 22.79852074764148 92-93 18.164204711139895 29.22949766804479 29.452764265894775 23.15353335492054 94-95 18.016551398425005 29.33937522585539 28.784978514667632 23.85909486105198 96-97 18.566654621045966 29.126489751085 28.3654689026201 23.941386725248933 98-99 18.84970079698024 29.274667477660405 27.927940821310056 23.947690904049303 100-101 18.91699915907749 28.898212592367283 27.618042260829114 24.56674598772611 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 898.0 1 603.0 2 373.0 3 459.0 4 459.0 5 462.5 6 709.0 7 1334.5 8 2002.0 9 2289.0 10 2050.5 11 1647.0 12 1560.0 13 1631.5 14 1601.5 15 1580.0 16 1619.5 17 1614.0 18 1624.0 19 1715.0 20 1755.0 21 1794.0 22 1986.0 23 2236.5 24 2431.5 25 2730.5 26 3083.5 27 3438.0 28 3904.5 29 4392.5 30 4864.5 31 5324.5 32 5822.5 33 6139.5 34 6459.5 35 6807.5 36 7407.5 37 7919.5 38 8112.0 39 8139.5 40 7895.5 41 7928.0 42 8047.0 43 8059.5 44 8243.0 45 8425.0 46 8380.5 47 8441.5 48 9181.5 49 9057.0 50 8255.0 51 7781.5 52 7109.5 53 6714.5 54 6354.5 55 5872.5 56 5303.5 57 4852.0 58 4563.0 59 4101.0 60 3350.0 61 2729.5 62 2276.0 63 1679.0 64 1108.5 65 783.0 66 584.0 67 432.0 68 332.0 69 246.5 70 191.0 71 160.0 72 127.0 73 100.5 74 79.5 75 65.5 76 56.0 77 34.0 78 16.0 79 15.5 80 13.5 81 8.5 82 4.0 83 1.5 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0407881413845785 2 0.0 3 0.0 4 0.0 5 3.577907138998114E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.008944767847495285 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.577907138998114E-4 24-25 0.0 26-27 0.0016100582125491516 28-29 0.003220116425098303 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01001813998919472 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.366860708497171E-4 84-85 1.788953569499057E-4 86-87 0.0 88-89 0.0 90-91 0.009839244632244814 92-93 0.0023256396403487744 94-95 0.0 96-97 0.0 98-99 1.788953569499057E-4 100-101 0.013596047128192835 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 279493.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.33425855454174 #Duplication Level Percentage of deduplicated Percentage of total 1 80.74174328355113 49.52234958697759 2 11.305257679745933 13.867991951105116 3 3.5222335026873552 6.481007410298864 4 1.573329262585443 3.859959351313682 5 0.8100052808611264 2.484053666344027 6 0.45721360498070784 1.6825714477524512 7 0.3213889208472425 1.3798505817466946 8 0.21950696743724907 1.0770637676255688 9 0.16352418187778056 0.9026671006090531 >10 0.8196498233808194 8.76164194578842 >50 0.04156169854164777 1.7287585870700908 >100 0.020488161252924957 2.3430692708659215 >500 0.0017561281073935676 0.7974190699652486 >1k 0.0017561281073935676 3.23635096653163 >5k 5.85376035797856E-4 1.875245296005625 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5223 1.868740898698715 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4244 1.5184637897907998 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3018 1.0798123745496309 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1752 0.6268493307524696 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 899 0.3216538517959305 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 800 0.2862325711198491 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 522 0.18676675265570158 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 445 0.1592168676854161 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 381 0.13631826199582817 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 371 0.13274035485683003 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 282 0.10089698131974684 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.788953569499057E-4 0.0 22-23 0.0 0.0 0.0 7.155814277996228E-4 0.0 24-25 0.0 0.0 0.0 8.944767847495285E-4 0.0 26-27 0.0 0.0 0.0 0.0017889535694990573 0.0 28-29 0.0 0.0 0.0 0.004651279280697549 0.0 30-31 0.0 0.0 0.0 0.014669419269892268 0.0 32-33 0.0 0.0 0.0 0.02343529176043765 0.0 34-35 0.0 0.0 0.0 0.03220116425098303 0.0 36-37 0.0 0.0 0.0 0.03846250174422973 0.0 38-39 0.0 0.0 0.0 0.04740726959172502 0.0 40-41 0.0 0.0 0.0 0.06458122385891597 0.0 42-43 0.0 0.0 0.0 0.10375930703094532 0.0 44-45 0.0 0.0 0.0 0.1559967512603178 0.0 46-47 0.0 0.0 0.0 0.21199099798563828 0.0 48-49 0.0 0.0 0.0 0.3318508871420751 0.0 50-51 0.0 0.0 0.0 0.48874211518714245 0.0 52-53 0.0 0.0 0.0 0.6322161914609667 0.0 54-55 0.0 0.0 0.0 0.8281066073211136 0.0 56-57 0.0 0.0 0.0 1.0547670245766443 0.0 58-59 0.0 0.0 0.0 1.3467242471188903 0.0 60-61 0.0 0.0 0.0 1.687162111394561 0.0 62-63 0.0 0.0 0.0 2.110070735224138 0.0 64-65 0.0 0.0 0.0 2.4995259273040826 0.0 66-67 0.0 0.0 0.0 2.9608970528778897 0.0 68-69 0.0 0.0 0.0 3.562879929014322 0.0 70-71 0.0 0.0 0.0 4.221393737946926 0.0 72-73 0.0 0.0 0.0 4.95343353858594 0.0 74-75 0.0 0.0 0.0 5.740573109165524 0.0 76-77 0.0 0.0 0.0 6.5169789583281155 0.0 78-79 0.0 0.0 0.0 7.339360914226832 0.0 80-81 0.0 0.0 0.0 8.297345550693578 0.0 82-83 0.0 0.0 0.0 9.42850089268783 0.0 84-85 0.0 0.0 0.0 10.526918384360252 0.0 86-87 0.0 0.0 0.0 11.580075350724346 0.0 88-89 0.0 0.0 0.0 12.94021674961448 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1970 0.0 53.05938 1 TCGAGAA 40 5.37904E-4 47.503754 9 ATCGAGA 40 5.37904E-4 47.503754 8 GATCGAG 45 9.608808E-4 42.225563 7 GTATCAA 3415 0.0 40.068905 1 TTCGATA 25 0.001615898 37.99621 16-17 ACAGTAC 105 1.8936989E-6 31.669172 8 AGATCGA 60 0.0039478103 31.663504 6 CGATAAG 30 0.0039511616 31.663504 18-19 GAGATCG 60 0.0039478103 31.663504 5 GTTATTC 80 4.48766E-4 29.684536 3 TATCAAC 4625 0.0 29.575426 2 GTATAGA 65 0.005829245 29.238316 1 ATCAACG 4675 0.0 29.157516 3 TCAACGC 4775 0.0 28.54689 4 CAACGCA 4815 0.0 28.408377 5 AACGCAG 4915 0.0 27.830387 6 GCACCGC 35 0.008377391 27.149864 88-89 CCGTATC 35 0.008388369 27.142576 92-93 TAAGTCG 35 0.008392031 27.140146 22-23 >>END_MODULE