FastQCFastQC Report
Thu 26 May 2016
SRR937182_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937182_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259993
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57132.197366852184482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41471.5950429434638627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30051.1558003484709205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10090.3880873715830811No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8550.32885500763482095No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.3277011304150496No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.19154361848203605No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4790.18423572942348448No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4250.16346593946760105No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4080.15692730188889703No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.14038839507217502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.056.8971251
GTATCAA31900.046.0714531
GCGGTAT459.549317E-442.2777251
GGGATAG459.6130045E-442.2206767
TCAACGC41600.035.2077034
ATCAACG41600.035.086623
TATCAAC41750.035.074442
CAACGCA42150.034.6354755
AACGCAG42850.034.0302626
GACATAT702.0332092E-433.973171
TTAGACT753.0517936E-431.7021734
CGCAGAG47150.030.7252838
ACGCAGA47250.030.6602557
GTGGTAT6400.029.7265241
GCAGAGT49700.029.3399759
GTGTATC650.005798486529.2691961
CATATAG1004.9073875E-528.5264513
TGGTATC6900.028.2508352
TTTAGAC856.359102E-427.967113
TACACTA1205.3140866E-627.73945
Produced by FastQC (version 0.11.5)