##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937182_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 259993 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.947267811056452 33.0 31.0 34.0 31.0 34.0 2 32.01506579023281 33.0 31.0 34.0 30.0 34.0 3 31.99854611470309 33.0 31.0 34.0 30.0 34.0 4 35.66788721234803 37.0 35.0 37.0 35.0 37.0 5 35.698664964056725 37.0 35.0 37.0 35.0 37.0 6 35.78743273857373 37.0 35.0 37.0 35.0 37.0 7 35.75285103829719 37.0 35.0 37.0 35.0 37.0 8 35.76751297150308 37.0 35.0 37.0 35.0 37.0 9 37.39446446635102 39.0 37.0 39.0 35.0 39.0 10-11 37.303702407372505 39.0 37.0 39.0 35.0 39.0 12-13 37.161221648275145 39.0 37.0 39.0 34.0 39.0 14-15 38.226284938440656 40.0 38.0 41.0 33.5 41.0 16-17 38.24552391795164 40.0 38.0 41.0 34.0 41.0 18-19 38.380391010527205 40.0 38.0 41.0 34.0 41.0 20-21 38.426403787794285 40.0 38.0 41.0 34.0 41.0 22-23 38.291909782186444 40.0 38.0 41.0 34.0 41.0 24-25 38.13890566284477 40.0 38.0 41.0 34.0 41.0 26-27 37.95696999534603 40.0 38.0 41.0 34.0 41.0 28-29 37.896547214732706 40.0 37.5 41.0 34.0 41.0 30-31 37.53014888862393 39.5 36.5 41.0 33.0 41.0 32-33 37.098369956114205 39.0 35.0 41.0 33.0 41.0 34-35 36.86139626836108 39.0 35.0 41.0 33.0 41.0 36-37 36.74060455473801 39.0 35.0 41.0 32.5 41.0 38-39 36.565024827591515 39.0 35.0 41.0 31.5 41.0 40-41 36.22846961264342 39.0 35.0 41.0 31.0 41.0 42-43 35.78106141319189 39.0 35.0 41.0 30.0 41.0 44-45 35.21502886616178 39.0 35.0 41.0 26.5 41.0 46-47 34.409620643632714 38.5 35.0 41.0 20.5 41.0 48-49 33.75308950625593 38.0 34.5 40.5 7.0 41.0 50-51 32.71726161858204 37.5 33.0 39.5 2.0 40.5 52-53 32.68783774947787 38.0 33.0 39.5 2.0 40.5 54-55 32.90409357175001 38.0 34.0 40.0 2.0 41.0 56-57 32.66224859900074 38.0 33.0 40.0 2.0 41.0 58-59 32.37837941790741 38.0 33.0 40.0 2.0 41.0 60-61 32.0575015481186 37.0 33.0 40.0 2.0 41.0 62-63 31.678689426253783 37.0 32.5 40.0 2.0 41.0 64-65 31.242673841218803 36.0 32.0 40.0 2.0 41.0 66-67 30.822525991084376 35.5 31.0 39.0 2.0 41.0 68-69 30.342886154627237 35.0 31.0 39.0 2.0 41.0 70-71 29.889573950067884 35.0 30.0 38.5 2.0 40.0 72-73 29.482691841703428 35.0 29.5 38.0 2.0 40.0 74-75 29.033277819018203 35.0 29.0 37.0 2.0 39.5 76-77 28.621912897654937 35.0 28.5 37.0 2.0 39.0 78-79 28.18980895639498 35.0 27.5 36.0 2.0 39.0 80-81 27.730310046808953 34.0 26.5 36.0 2.0 37.5 82-83 27.27124191805164 34.0 26.0 35.0 2.0 37.0 84-85 26.837522548684003 34.0 25.0 35.0 2.0 37.0 86-87 26.395927967291428 34.0 23.5 35.0 2.0 36.0 88-89 26.000973103122007 34.0 21.0 35.0 2.0 36.0 90-91 25.579998307646747 33.0 18.5 35.0 2.0 35.5 92-93 25.15076175127792 33.0 11.0 35.0 2.0 35.0 94-95 24.657069613412666 33.0 2.0 35.0 2.0 35.0 96-97 24.090492820960563 32.5 2.0 35.0 2.0 35.0 98-99 23.423701791971325 32.0 2.0 35.0 2.0 35.0 100-101 21.850184428042294 30.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07474568702267703 1101 2 0.08896315562870072 1101 3 0.10898969218701993 1101 4 0.054914585262061166 1101 5 0.0907655293428391 1101 6 0.05168091700785027 1101 7 0.05200409474475265 1101 8 0.2197392030068741 1101 9 0.18264424531722057 1101 10-11 0.29674359887530244 1101 12-13 0.2207236103875445 1101 14-15 0.23244880403600376 1101 16-17 0.2197203731406745 1101 18-19 0.08695068448866294 1101 20-21 -0.15238271522910907 1101 22-23 0.017037106855696038 1101 24-25 -0.10449465072394304 1101 26-27 -0.13715440515796473 1101 28-29 -0.07872719183030341 1101 30-31 0.06822731306476726 1101 32-33 0.13061956433476496 1101 34-35 0.3290699352702475 1101 36-37 0.4720123167752419 1101 38-39 0.8239253636626032 1101 40-41 0.5817758469410492 1101 42-43 0.6797864788791728 1101 44-45 0.8673275037725361 1101 46-47 1.2812011693322134 1101 48-49 1.6179384386863305 1101 50-51 1.401277421143888 1101 52-53 1.696733977670604 1101 54-55 1.824796441212456 1101 56-57 1.7754767369624496 1101 58-59 2.145569650599761 1101 60-61 1.8016482987679545 1101 62-63 2.004784963658974 1101 64-65 2.008528336489089 1101 66-67 2.040688889928351 1101 68-69 2.232928324561037 1101 70-71 2.3888879705412833 1101 72-73 2.6719221076327173 1101 74-75 2.604630681341053 1101 76-77 2.590859517697016 1101 78-79 2.417684364683492 1101 80-81 2.298449269911462 1101 82-83 2.189811889493477 1101 84-85 2.2891908007633894 1101 86-87 2.7425215069316344 1101 88-89 2.680733455607232 1101 90-91 2.56262121118284 1101 92-93 2.7331532852046507 1101 94-95 2.8795666741121018 1101 96-97 2.8189177436276225 1101 98-99 2.888291257092174 1101 100-101 3.088009466363527 1102 1 -0.14823064031845945 1102 2 0.023451815745755056 1102 3 0.02924470890942743 1102 4 -0.1181688700054977 1102 5 -0.22255752667722817 1102 6 -0.17621414988177975 1102 7 -0.09054136930207335 1102 8 -0.06857209507004569 1102 9 0.018033041303844755 1102 10-11 0.07482222032178498 1102 12-13 -0.02585352171947619 1102 14-15 -0.032114714985738146 1102 16-17 0.08632977426943711 1102 18-19 0.17651028419602 1102 20-21 -0.017006330404697678 1102 22-23 -0.057188175334943026 1102 24-25 0.02270669752019927 1102 26-27 -0.2789356029004395 1102 28-29 0.01791983471651548 1102 30-31 0.09998682393433 1102 32-33 0.32910670898359484 1102 34-35 0.5098613754876382 1102 36-37 0.2744331466247374 1102 38-39 0.7454835524167791 1102 40-41 0.7455574627437258 1102 42-43 1.11313527173035 1102 44-45 1.0359658033126706 1102 46-47 1.2401136902352263 1102 48-49 1.496808761637837 1102 50-51 1.633918124530176 1102 52-53 1.5964942462742826 1102 54-55 1.6213742265385491 1102 56-57 1.2194044125477355 1102 58-59 1.6699706487184898 1102 60-61 1.616983269503745 1102 62-63 1.3987054538345625 1102 64-65 1.8768295749924313 1102 66-67 1.888534573456777 1102 68-69 1.7278952716764202 1102 70-71 1.8716103263105133 1102 72-73 1.9430080190039547 1102 74-75 2.214671964785868 1102 76-77 2.4008805513508626 1102 78-79 2.3860456993406807 1102 80-81 2.3872968608395553 1102 82-83 2.4910895859817686 1102 84-85 2.4582471605041896 1102 86-87 2.242377798737653 1102 88-89 2.3296478055236207 1102 90-91 2.376950224560769 1102 92-93 2.180333991726389 1102 94-95 2.052904884814442 1102 96-97 2.0570258905757832 1102 98-99 2.307837531130634 1102 100-101 2.785030134214697 1103 1 -0.05803023270976482 1103 2 -0.4824415266455482 1103 3 -0.289505291090574 1103 4 -0.3905703645707135 1103 5 -0.3543849723293988 1103 6 -0.33042610640352166 1103 7 -0.3707383801716375 1103 8 -0.051163807026568975 1103 9 -0.2365865239135445 1103 10-11 -0.23887513022169315 1103 12-13 -0.41681819563252276 1103 14-15 -0.22515989161617256 1103 16-17 -0.4003465164914317 1103 18-19 -0.4399723924344201 1103 20-21 -0.5702756918177414 1103 22-23 -0.915153528595809 1103 24-25 -0.7779896340015355 1103 26-27 -0.562070793117833 1103 28-29 -0.6712021082182673 1103 30-31 -0.40785964345697323 1103 32-33 -0.35625334536423026 1103 34-35 -0.2502778908167116 1103 36-37 -0.3274860109839537 1103 38-39 -0.4585161079093112 1103 40-41 -0.288291042691057 1103 42-43 -0.4561666983783468 1103 44-45 -0.05650192766558604 1103 46-47 0.05101722284391741 1103 48-49 0.027302987181315075 1103 50-51 0.11498979572582613 1103 52-53 0.13762026529602167 1103 54-55 0.10631818034289608 1103 56-57 0.045627238634690315 1103 58-59 0.18336229274022742 1103 60-61 0.020210171677657485 1103 62-63 0.06506924459543129 1103 64-65 0.39757515923156106 1103 66-67 0.6144347093263427 1103 68-69 0.6979784917851148 1103 70-71 0.6881354621800817 1103 72-73 0.5735107363952601 1103 74-75 0.6206841930467384 1103 76-77 0.6087524942856426 1103 78-79 0.7811459031450276 1103 80-81 0.8290512766004241 1103 82-83 0.8113001838078553 1103 84-85 0.6886055164824505 1103 86-87 0.5691101356941743 1103 88-89 0.6045628870453612 1103 90-91 0.8575208767346822 1103 92-93 0.6418489373785619 1103 94-95 0.9087472761187882 1103 96-97 0.9935832954670865 1103 98-99 0.9280905881958503 1103 100-101 1.0194475934538296 1104 1 0.11651010905964654 1104 2 -0.12705541373185625 1104 3 -0.08627146024480226 1104 4 0.049382047283060615 1104 5 0.027228698447153477 1104 6 -0.14737397575242994 1104 7 0.12099858716558742 1104 8 0.21208516550209566 1104 9 0.3583626432938942 1104 10-11 0.23593559874218073 1104 12-13 0.16751557032032593 1104 14-15 0.3715359768674915 1104 16-17 0.26692581749082933 1104 18-19 0.3260020830144228 1104 20-21 0.3955257358266451 1104 22-23 0.511695919814315 1104 24-25 0.30742568072915333 1104 26-27 0.47591319996025305 1104 28-29 0.34357184808715857 1104 30-31 0.5930488575164219 1104 32-33 0.2774314913219058 1104 34-35 0.40585989849758874 1104 36-37 0.5352870800824476 1104 38-39 0.49398226976752113 1104 40-41 0.7140451026690968 1104 42-43 0.9297407210462652 1104 44-45 0.9197742998922749 1104 46-47 0.9326471168521451 1104 48-49 1.280104198515943 1104 50-51 0.9956300726023173 1104 52-53 1.5382746023066218 1104 54-55 1.3715734258544714 1104 56-57 0.9702595120502231 1104 58-59 1.195832550148836 1104 60-61 1.3510667583097167 1104 62-63 1.3598615872300819 1104 64-65 1.726414657392926 1104 66-67 1.5226977419891163 1104 68-69 1.479484007683883 1104 70-71 1.5077169028030504 1104 72-73 1.571234076217884 1104 74-75 1.4641083764774088 1104 76-77 1.485520826537428 1104 78-79 1.4680429397013768 1104 80-81 1.4528237187375659 1104 82-83 1.560916233991719 1104 84-85 1.5951651098201118 1104 86-87 1.652378576100837 1104 88-89 1.4586998111206348 1104 90-91 1.4698451250582814 1104 92-93 1.3776482019253926 1104 94-95 1.5187786410333466 1104 96-97 1.2694160880260306 1104 98-99 1.4257003653968034 1104 100-101 1.9001527632569868 1105 1 -0.08221501531846087 1105 2 -0.21287630925424494 1105 3 0.01049470890942672 1105 4 -0.021684495005494853 1105 5 -0.20302627667722817 1105 6 0.039410850118223095 1105 7 -0.08351011930207619 1105 8 -0.05333772007004711 1105 9 -0.3913419586961524 1105 10-11 -0.05545121717821644 1105 12-13 -0.3227285217194762 1105 14-15 0.04073684751426754 1105 16-17 0.1751969617694371 1105 18-19 0.12436184669602568 1105 20-21 -0.057436017904691994 1105 22-23 0.005702449665058396 1105 24-25 0.10747232252020211 1105 26-27 0.2487987720995548 1105 28-29 0.2458495222165169 1105 30-31 0.30389307393433285 1105 32-33 0.40332545898359484 1105 34-35 0.6821270004876325 1105 36-37 0.3623237716247374 1105 38-39 0.4843507399167777 1105 40-41 0.4287605877437315 1105 42-43 0.2414555842303443 1105 44-45 0.5111611158126763 1105 46-47 1.1065199402352235 1105 48-49 0.9220040741378313 1105 50-51 1.2270821870301774 1105 52-53 1.1459083087742812 1105 54-55 1.4926632890385463 1105 56-57 1.1709669125477298 1105 58-59 1.3729003362184926 1105 60-61 1.4505770195037506 1105 62-63 1.6067132663345625 1105 64-65 1.5121811374924299 1105 66-67 1.7975189484567764 1105 68-69 2.0222312091764216 1105 70-71 2.1634072013105126 1105 72-73 2.332070519003956 1105 74-75 2.4513907147858696 1105 76-77 2.629200863850862 1105 78-79 2.409678511840678 1105 80-81 2.1519452983395553 1105 82-83 2.2262458359817714 1105 84-85 2.167817473004188 1105 86-87 2.2869090487376518 1105 88-89 2.0628509305236236 1105 90-91 2.2191377245607704 1105 92-93 2.1576777417263884 1105 94-95 1.943529884814442 1105 96-97 1.6787055780757818 1105 98-99 1.6201422186306331 1105 100-101 1.5231160717146963 1106 1 -0.4973211038793437 1106 2 -0.5311419919110705 1106 3 -0.5889517855555155 1106 4 -0.6196895688062369 1106 5 -0.5204847637620986 1106 6 -0.46282623475262596 1106 7 -0.42836713037218743 1106 8 -0.7180978676715242 1106 9 -0.8128018295448669 1106 10-11 -0.6961108112741599 1106 12-13 -0.8895239552988059 1106 14-15 -0.5954831709359212 1106 16-17 -0.48601152531543335 1106 18-19 -0.11739899278737198 1106 20-21 -0.14750174668698435 1106 22-23 -0.17913957063014152 1106 24-25 -0.2614045224982533 1106 26-27 -0.6131938478266363 1106 28-29 -0.5577655608093082 1106 30-31 -0.6613571566929721 1106 32-33 -0.6686590889868853 1106 34-35 -0.6083999829071942 1106 36-37 -0.974911006973052 1106 38-39 -0.9701637896035109 1106 40-41 -1.2246699934370824 1106 42-43 -1.2321894803452977 1106 44-45 -1.0623859321578095 1106 46-47 -1.0575540911300934 1106 48-49 -1.4813284904378108 1106 50-51 -1.3745898609403042 1106 52-53 -1.4105146617054203 1106 54-55 -1.1123124950942938 1106 56-57 -1.0815065653120435 1106 58-59 -1.033684073375607 1106 60-61 -1.2846109417508664 1106 62-63 -1.4465904698278003 1106 64-65 -1.0710234289282319 1106 66-67 -0.7554273153808602 1106 68-69 -0.8879913461741324 1106 70-71 -0.754881544076941 1106 72-73 -0.7662574330255651 1106 74-75 -0.6307356689779695 1106 76-77 -0.3434294405771787 1106 78-79 -0.5075954733991708 1106 80-81 -0.9921102828412565 1106 82-83 -0.7642292562691537 1106 84-85 -0.965289076811306 1106 86-87 -0.6665388294911274 1106 88-89 -1.1275203794394812 1106 90-91 -0.4839238529300083 1106 92-93 -0.23135016417767318 1106 94-95 -0.10766937717817981 1106 96-97 -0.2155575676806727 1106 98-99 -0.5330116097826512 1106 100-101 -0.3728594817908366 1107 1 0.04486831801487412 1107 2 -0.18640572101895003 1107 3 -0.0767601930513564 1107 4 0.08965128930822885 1107 5 -0.11099196295173641 1107 6 0.03781771286331548 1107 7 0.3041124297175344 1107 8 0.2652897309103466 1107 9 0.04683206091169012 1107 10-11 0.22408309654727532 1107 12-13 0.41167711553542574 1107 14-15 0.2475383181024995 1107 16-17 0.03328519706355593 1107 18-19 0.15576503297052824 1107 20-21 0.20870981542863376 1107 22-23 -0.09965906994278839 1107 24-25 0.20545026369666886 1107 26-27 0.25468112504074014 1107 28-29 0.1573997673145513 1107 30-31 0.2803330249147251 1107 32-33 -0.12072478611444382 1107 34-35 -0.04241344068883279 1107 36-37 -0.20097279700271287 1107 38-39 -0.2456431326322459 1107 40-41 0.07364416617509306 1107 42-43 0.2674053391323099 1107 44-45 0.26594052757737785 1107 46-47 0.41126871474502735 1107 48-49 0.25356044668685485 1107 50-51 0.25961895173605853 1107 52-53 0.7100627205389927 1107 54-55 0.6572772596267811 1107 56-57 0.7868676478418486 1107 58-59 0.7111969048459414 1107 60-61 0.9536713822488387 1107 62-63 1.0985943937855431 1107 64-65 1.1140500100414492 1107 66-67 1.217034879829324 1107 68-69 1.0763672385881868 1107 70-71 1.003970926800708 1107 72-73 1.3269847346902282 1107 74-75 1.2899936559623377 1107 76-77 0.9958368932626307 1107 78-79 1.0713206687034251 1107 80-81 1.0199293669670055 1107 82-83 0.815584071275886 1107 84-85 0.9387120808473277 1107 86-87 1.3232448330513797 1107 88-89 0.7054367148373473 1107 90-91 0.7865703225999816 1107 92-93 1.048455928000898 1107 94-95 1.3048043946183618 1107 96-97 1.292155332977746 1107 98-99 0.6331630519639653 1107 100-101 0.8609224442637178 1108 1 -0.3324112036592659 1108 2 -0.16915433616007292 1108 3 -0.4731375780860887 1108 4 -0.14145747706827905 1108 5 -0.07984802555615289 1108 6 -0.10859923956788009 1108 7 -0.1577533928446755 1108 8 0.009666764234886216 1108 9 0.028543130989945098 1108 10-11 -0.06629762973427233 1108 12-13 -0.02665929526207833 1108 14-15 0.0498596053617959 1108 16-17 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-1.6582053189848693 2316 74-75 -1.788448670688993 2316 76-77 -2.0657799322385237 2316 78-79 -2.218010035645353 2316 80-81 -1.8041039335040203 2316 82-83 -1.5901996947444879 2316 84-85 -1.6036036163812852 2316 86-87 -1.7390511467930736 2316 88-89 -2.144568762213808 2316 90-91 -2.22421946817666 2316 92-93 -1.8136647862065693 2316 94-95 -2.2010231878112556 2316 96-97 -2.427518234773377 2316 98-99 -3.0649747506431098 2316 100-101 -3.0297994310785974 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1120.0 3 190.0 4 165.0 5 141.0 6 189.0 7 206.0 8 287.0 9 306.0 10 456.0 11 752.0 12 1251.0 13 1820.0 14 2201.0 15 3184.0 16 4722.0 17 6769.0 18 5733.0 19 2994.0 20 2749.0 21 2593.0 22 2712.0 23 2818.0 24 2811.0 25 3007.0 26 2975.0 27 3315.0 28 3678.0 29 4342.0 30 5251.0 31 6362.0 32 8013.0 33 10668.0 34 14908.0 35 22822.0 36 31404.0 37 45919.0 38 43614.0 39 7546.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.8157057715468 19.542661272890097 16.1076027545317 18.534030201031406 2 14.919614644139992 21.875928376590057 42.00602649032174 21.19843048894822 3 17.809841631141374 29.63214444165815 31.846222102197103 20.711791825003374 4 11.056451332862615 17.91696086488489 39.287905270102904 31.738682532149596 5 11.004876994783467 39.552659196839215 33.655894064265205 15.786569744112109 6 26.577729796421963 38.883405305367056 18.2703578038248 16.26850709438618 7 24.14120114262364 38.00804154153961 21.07146023754544 16.779297078291307 8 26.5130516243692 32.99703538645993 20.170166504107666 20.319746485063206 9 28.510078911963237 15.413468618327467 20.86791594545938 35.20853652424991 10-11 23.771792335883926 28.6728628907101 26.179528554876295 21.37581621852968 12-13 26.489332171579704 27.15515579578839 27.949941783805876 18.405570248826038 14-15 22.859015382242955 26.56309032730637 29.86410424457381 20.713790045876866 16-17 18.45154450387933 30.64083658618862 29.788443930412996 21.11917497951906 18-19 18.147859022175965 26.363855398463777 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44-45 17.957386768850014 28.21170148379163 34.98706645644036 18.843845290917994 46-47 18.341718228955838 28.484165076427843 33.961140345804665 19.212976348811658 48-49 18.514748801936946 28.945704515049524 33.09796165427158 19.441585028741947 50-51 18.69062490361186 29.309321119027793 32.545263872181614 19.454790105178745 52-53 18.60892869193024 29.702409069437884 32.10781525647576 19.58084698215611 54-55 18.45443259540278 29.301896103695825 32.18383492562863 20.05983637527277 56-57 18.669200348445486 29.36597568590569 31.71258643683655 20.252237528812277 58-59 18.792794772027804 28.718387515152216 32.25162410556156 20.237193607258416 60-61 18.899856238220593 28.516478645787164 32.73451862929117 19.849146486701073 62-63 18.959419479806108 28.442212992319476 32.88785668166794 19.71051084620648 64-65 18.83991220040508 28.269889325488666 33.07400719586671 19.816191278239536 66-67 18.82961461228045 28.26427137092267 32.81519196154988 20.090922055247002 68-69 18.85302131628339 28.063228158613807 32.86777429636224 20.215976228740562 70-71 18.801132454377083 28.070838143437797 32.76890491507556 20.35912448710956 72-73 18.652864719849518 27.894978260199206 32.651716056615996 20.800440963335287 74-75 18.404639562718515 28.052470694353964 32.57812590345423 20.96476383947329 76-77 18.162053812350628 27.798165137614678 32.870634492329046 21.169146557705652 78-79 18.15951369562033 27.59553784123411 33.09402295837734 21.15092550476822 80-81 18.133179146188684 27.855256817962804 32.60152259805339 21.410041437795122 82-83 17.967723160771346 27.619168757684733 32.41174361998589 22.00136446155803 84-85 17.960299765483036 27.28567326795635 32.494763353688974 22.259263612871646 86-87 18.085007978169877 27.39653431383885 32.13217553515405 22.38628217283722 88-89 17.926319055410058 26.9684899010046 32.20674756654777 22.898443477037578 90-91 18.104973461765283 27.218746747765355 32.23730616681507 22.4389736236543 92-93 18.334749821252714 27.522071194417304 31.655903285673816 22.487275698656166 94-95 18.270783628255483 27.64977047335294 31.160314052581068 22.91913184581051 96-97 18.479475814222393 27.793409134708035 30.675467334746582 23.05164771632299 98-99 18.883644556312117 27.763762197249005 30.312006737757457 23.040586508681415 100-101 19.4924317605912 27.273025079729063 29.95481216699265 23.279730992687085 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 740.0 1 498.5 2 344.5 3 438.0 4 352.5 5 333.5 6 651.0 7 1453.0 8 2494.5 9 2809.5 10 2177.5 11 1593.5 12 1471.5 13 1491.0 14 1504.0 15 1486.5 16 1444.5 17 1446.0 18 1521.5 19 1568.0 20 1617.5 21 1746.0 22 1907.5 23 2116.0 24 2355.0 25 2683.0 26 3016.5 27 3297.0 28 3657.5 29 4153.5 30 4650.0 31 5056.0 32 5407.0 33 5722.5 34 6164.5 35 6567.5 36 7063.0 37 7525.5 38 7616.5 39 7437.5 40 7365.5 41 7416.5 42 7306.0 43 7320.5 44 7576.0 45 7811.0 46 7897.5 47 8015.5 48 8595.5 49 8462.5 50 7650.5 51 7015.0 52 6329.5 53 6141.0 54 5872.5 55 5342.5 56 4805.0 57 4180.0 58 3833.0 59 3411.0 60 2792.0 61 2376.5 62 2015.0 63 1466.5 64 985.0 65 713.0 66 506.5 67 401.0 68 298.0 69 189.0 70 150.5 71 130.0 72 91.0 73 82.5 74 66.5 75 49.5 76 37.5 77 23.0 78 15.0 79 10.5 80 6.0 81 5.5 82 8.0 83 4.0 84 0.5 85 1.5 86 2.0 87 1.5 88 0.5 89 0.5 90 1.5 91 1.0 92 1.0 93 1.0 94 0.0 95 0.0 96 2.0 97 2.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35731731238917974 2 0.3092390948987088 3 0.30308508305992854 4 0.3130853522979465 5 0.31423922951771777 6 0.2434680933717446 7 0.22692918655502262 8 0.23077544395426033 9 0.22692918655502262 10-11 0.24673741216109665 12-13 0.2365448300531168 14-15 0.23192932117403164 16-17 0.23289088552384102 18-19 0.22712149942498452 20-21 0.2240444935055944 22-23 0.22365986776567062 24-25 0.2153904143573096 26-27 0.2132749727877289 28-29 0.22789075090483205 30-31 0.24327578050178272 32-33 0.23192932117403164 34-35 0.24423734485159218 36-37 0.2215444261960899 38-39 0.21519810148734772 40-41 0.2165442915770809 42-43 0.2228906162858231 44-45 0.22692918655502262 46-47 0.23000619247441276 48-49 0.2369294557930406 50-51 0.24039108745235446 52-53 0.25404530121964825 54-55 0.2380833330128119 56-57 0.21346728565769077 58-59 0.20865946390864368 60-61 0.2061593965991392 62-63 0.21942898462650917 64-65 0.20712096094894863 66-67 0.20769789955883428 68-69 0.20000538476035892 70-71 0.2140442242675764 72-73 0.2157750400972334 74-75 0.22000592323639484 76-77 0.2203905489763186 78-79 0.218082794536776 80-81 0.21846742027669974 82-83 0.21154415695807194 84-85 0.20135157485009209 86-87 0.20577477085921544 88-89 0.2063517094691011 90-91 0.21385191139761456 92-93 0.2109672183481863 94-95 0.2100056539983769 96-97 0.20885177677860559 98-99 0.21519810148734772 100-101 0.20019769763032083 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 259993.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.547873816728874 #Duplication Level Percentage of deduplicated Percentage of total 1 82.17229506076903 52.21874637752764 2 10.700736743928294 13.600181366983788 3 3.255825112267532 6.207022902111427 4 1.3835682398700655 3.5169127969638634 5 0.6938875550313375 2.2047539395064977 6 0.42249145907938235 1.6109060358133354 7 0.2726753401025329 1.2129556674038575 8 0.1922868949902914 0.9775538671562908 9 0.14740153748193624 0.8430348873864525 >10 0.7014866542108809 7.702671704620569 >50 0.03319996973453965 1.4423274459382973 >100 0.020523617654079056 2.4860968556186447 >500 0.0012072716267105326 0.6548013165469876 >1k 0.001810907440065799 3.1305410335910757 >5k 6.036358133552663E-4 2.191493802831248 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5713 2.197366852184482 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4147 1.5950429434638627 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3005 1.1558003484709205 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1009 0.3880873715830811 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 855 0.32885500763482095 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 852 0.3277011304150496 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 498 0.19154361848203605 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 479 0.18423572942348448 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 425 0.16346593946760105 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 408 0.15692730188889703 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 365 0.14038839507217502 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.9231286996188358E-4 0.0 22-23 0.0 0.0 0.0 0.0011538772197713014 0.0 24-25 0.0 0.0 0.0 0.0015385029596950686 0.0 26-27 0.0 0.0 0.0 0.0015385029596950686 0.0 28-29 0.0 0.0 0.0 0.0034616316593139044 0.0 30-31 0.0 0.0 0.0 0.009615643498094179 0.0 32-33 0.0 0.0 0.0 0.016923532556645755 0.0 34-35 0.0 0.0 0.0 0.02403910874523545 0.0 36-37 0.0 0.0 0.0 0.03173162354371079 0.0 38-39 0.0 0.0 0.0 0.03961645121214802 0.0 40-41 0.0 0.0 0.0 0.057501548118603196 0.0 42-43 0.0 0.0 0.0 0.08519460139311444 0.0 44-45 0.0 0.0 0.0 0.13173431592389026 0.0 46-47 0.0 0.0 0.0 0.19423599866150243 0.0 48-49 0.0 0.0 0.0 0.3090467820287469 0.0 50-51 0.0 0.0 0.0 0.44962748997088386 0.0 52-53 0.0 0.0 0.0 0.5992469028012293 0.0 54-55 0.0 0.0 0.0 0.7809825649152092 0.0 56-57 0.0 0.0 0.0 1.0131041989592027 0.0 58-59 0.0 0.0 0.0 1.2969579950229428 0.0 60-61 0.0 0.0 0.0 1.635428646155858 0.0 62-63 0.0 0.0 0.0 2.078709811418 0.0 64-65 0.0 0.0 0.0 2.459489293942529 0.0 66-67 0.0 0.0 0.0 2.89450100579631 0.0 68-69 0.0 0.0 0.0 3.4916324670279586 0.0 70-71 0.0 0.0 0.0 4.110302969695338 0.0 72-73 0.0 0.0 0.0 4.831091606312477 0.0 74-75 0.0 0.0 0.0 5.649575180870254 0.0 76-77 0.0 0.0 0.0 6.432673187355045 0.0 78-79 0.0 0.0 0.0 7.2613493440208 0.0 80-81 0.0 0.0 0.0 8.23733715907736 0.0 82-83 0.0 0.0 0.0 9.42063824795283 0.0 84-85 0.0 0.0 0.0 10.583938798352264 0.0 86-87 0.0 0.0 0.0 11.6881992976734 0.0 88-89 0.0 0.0 0.0 13.109199093821758 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1605 0.0 56.897125 1 GTATCAA 3190 0.0 46.071453 1 GCGGTAT 45 9.549317E-4 42.277725 1 GGGATAG 45 9.6130045E-4 42.220676 7 TCAACGC 4160 0.0 35.207703 4 ATCAACG 4160 0.0 35.08662 3 TATCAAC 4175 0.0 35.07444 2 CAACGCA 4215 0.0 34.635475 5 AACGCAG 4285 0.0 34.030262 6 GACATAT 70 2.0332092E-4 33.97317 1 TTAGACT 75 3.0517936E-4 31.702173 4 CGCAGAG 4715 0.0 30.725283 8 ACGCAGA 4725 0.0 30.660255 7 GTGGTAT 640 0.0 29.726524 1 GCAGAGT 4970 0.0 29.339975 9 GTGTATC 65 0.0057984865 29.269196 1 CATATAG 100 4.9073875E-5 28.526451 3 TGGTATC 690 0.0 28.250835 2 TTTAGAC 85 6.359102E-4 27.96711 3 TACACTA 120 5.3140866E-6 27.7394 5 >>END_MODULE