FastQCFastQC Report
Thu 26 May 2016
SRR937182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937182_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259993
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47991.8458189258941586No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41531.5973506979034051No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29781.1454154534929788No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18230.7011727238810276No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.32231637005611685No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7890.3034697087998523No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.20462089363944413No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.15538879892920196No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.14692703265087906No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.1430807752516414No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2600.10000269238017946No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAGT250.005017681556.997816
GGTATCA19900.049.665441
GGGATCG459.604033E-442.228729
GTATCAA35500.040.9578441
CGGTATC500.001613821137.998542
TAATGTG550.002576456434.5441255
CCCCTAT600.00393869431.6776351
TATCAAC47050.030.8914782
ATCAACG47350.030.5954443
TCAACGC48050.030.1497254
TTAGGCA804.4851945E-429.686364
CAACGCA48900.029.5285155
GCGGTAT650.00582599529.2408921
GTGTAGG650.00582599529.2408921
AACGCAG49700.029.053216
GTAAGTA1004.9398714E-528.4989052
TACAGTC856.3879933E-427.9454777
CGCCTTA350.00838112427.14703438-39
TACTTGC350.00838505827.14442392-93
CTATACT1056.887439E-527.1418154
Produced by FastQC (version 0.11.5)