##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937182_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 259993 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0766712949964 31.0 31.0 34.0 31.0 34.0 2 32.26101087336967 33.0 31.0 34.0 31.0 34.0 3 32.27488817006612 34.0 31.0 34.0 30.0 34.0 4 35.99560757405007 37.0 35.0 37.0 35.0 37.0 5 35.88485074598162 37.0 35.0 37.0 35.0 37.0 6 36.001003873181205 37.0 35.0 37.0 35.0 37.0 7 35.959760455089175 37.0 35.0 37.0 35.0 37.0 8 35.96221052105249 37.0 35.0 37.0 35.0 37.0 9 37.60360855869197 39.0 37.0 39.0 35.0 39.0 10-11 37.52867769516872 39.0 37.0 39.0 35.0 39.0 12-13 37.43129622720612 39.0 37.0 39.0 34.5 39.0 14-15 38.424994134457464 40.0 38.0 41.0 34.0 41.0 16-17 38.44422349832496 40.0 38.0 41.0 34.0 41.0 18-19 38.60954141073029 40.0 38.0 41.0 34.0 41.0 20-21 38.61087029266173 40.0 38.0 41.0 34.0 41.0 22-23 38.4788186605024 40.0 38.0 41.0 34.0 41.0 24-25 38.310754520314006 40.0 38.0 41.0 34.0 41.0 26-27 38.05657460008538 40.0 37.0 41.0 33.5 41.0 28-29 37.93011734931325 40.0 37.0 41.0 33.0 41.0 30-31 37.773441977284 39.0 36.5 41.0 33.0 41.0 32-33 37.471489617028155 39.0 35.0 41.0 33.0 41.0 34-35 37.23597173770063 39.0 35.0 41.0 33.0 41.0 36-37 36.94287153884912 39.0 35.0 41.0 33.0 41.0 38-39 36.68818006638641 39.0 35.0 41.0 32.0 41.0 40-41 36.360061617043534 39.0 35.0 41.0 31.0 41.0 42-43 35.93745408530229 39.0 35.0 41.0 30.5 41.0 44-45 35.274720857869255 38.5 35.0 40.5 27.5 41.0 46-47 34.43804063955568 38.0 34.5 40.0 22.0 41.0 48-49 33.74336039816457 38.0 34.0 40.0 11.5 41.0 50-51 33.33643405784002 38.0 33.5 40.0 3.5 41.0 52-53 33.01978130180428 38.0 33.0 40.0 2.0 41.0 54-55 32.73976799375368 38.0 33.0 40.0 2.0 41.0 56-57 32.430194274461236 37.0 33.0 40.0 2.0 41.0 58-59 32.125841849588255 37.0 33.0 40.0 2.0 41.0 60-61 31.731056220744406 36.5 32.0 40.0 2.0 41.0 62-63 31.3082659917767 36.0 31.5 39.5 2.0 41.0 64-65 30.87997753785679 35.0 31.0 39.0 2.0 40.5 66-67 30.43336551368691 35.0 30.5 39.0 2.0 40.0 68-69 29.95484686126165 35.0 29.5 38.0 2.0 40.0 70-71 29.537906789798186 35.0 29.0 38.0 2.0 40.0 72-73 29.083867642590377 35.0 29.0 37.0 2.0 39.5 74-75 28.63246510482975 34.5 28.0 36.5 2.0 39.0 76-77 27.37971599235364 33.5 26.0 35.0 2.0 37.5 78-79 27.90150888677772 34.0 27.0 36.0 2.0 38.0 80-81 27.702034285538456 34.0 27.0 35.0 2.0 37.0 82-83 27.327533818218182 34.0 26.0 35.0 2.0 37.0 84-85 26.962648994396005 34.0 25.5 35.0 2.0 36.5 86-87 26.583821487501588 34.0 25.0 35.0 2.0 36.0 88-89 26.18863777101691 33.5 23.5 35.0 2.0 36.0 90-91 25.699359213517287 33.0 20.5 35.0 2.0 35.0 92-93 25.242806537099074 33.0 18.5 35.0 2.0 35.0 94-95 24.793946375479337 33.0 9.5 35.0 2.0 35.0 96-97 24.34042070363433 32.0 2.0 35.0 2.0 35.0 98-99 23.712738419880537 32.0 2.0 34.0 2.0 35.0 100-101 22.353507594435236 30.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23041599016548275 1101 2 0.24710125051974785 1101 3 0.2363404787825516 1101 4 0.08851924691735746 1101 5 -0.005993561734250363 1101 6 -0.11294118443698409 1101 7 -0.06287771089800742 1101 8 -0.1531252022867733 1101 9 -0.01390441324177516 1101 10-11 0.10703211811243563 1101 12-13 0.20626377149213937 1101 14-15 0.19771555900439353 1101 16-17 0.2605396649258722 1101 18-19 0.12167273867617467 1101 20-21 0.004629511884893134 1101 22-23 -0.04567196532739359 1101 24-25 0.020110761771697128 1101 26-27 0.18281121299209957 1101 28-29 0.1597015813382967 1101 30-31 0.3127911001693704 1101 32-33 0.4693198175080511 1101 34-35 0.46335965952978597 1101 36-37 0.47428686496093775 1101 38-39 0.38748850882682007 1101 40-41 0.5909260649081318 1101 42-43 0.8048438140474161 1101 44-45 0.8374116356967036 1101 46-47 1.0795884469652535 1101 48-49 1.0403585466865124 1101 50-51 1.2347387946403572 1101 52-53 1.4026583245920818 1101 54-55 1.3906435480166834 1101 56-57 1.529562652737809 1101 58-59 1.6224718617033496 1101 60-61 1.6644238380383207 1101 62-63 1.7740945205300953 1101 64-65 1.800948365760565 1101 66-67 1.8405981182104796 1101 68-69 1.449789680406127 1101 70-71 1.755611886987726 1101 72-73 1.72287209493593 1101 74-75 1.7870304279411222 1101 76-77 1.8340203152412613 1101 78-79 1.881798124727382 1101 80-81 1.4208146315199777 1101 82-83 1.534594427890898 1101 84-85 1.5566425477679218 1101 86-87 1.6074842342828255 1101 88-89 1.7856806960885798 1101 90-91 2.1285102777069547 1101 92-93 1.8527102223569472 1101 94-95 1.6437367233212221 1101 96-97 1.8819222278844983 1101 98-99 2.02262455145112 1101 100-101 1.7684675415918854 1102 1 0.09676736976414446 1102 2 0.17113187342944514 1102 3 0.16847755027084332 1102 4 0.1516463372183594 1102 5 -0.18084724066401492 1102 6 0.018042302185087067 1102 7 0.07120856627590655 1102 8 0.19318541644232567 1102 9 0.1764566862565502 1102 10-11 0.17244547513585218 1102 12-13 0.04484680869949642 1102 14-15 0.3172285188706141 1102 16-17 0.1589118430195171 1102 18-19 0.0712801540189858 1102 20-21 -0.043365758809088106 1102 22-23 0.11633289462243823 1102 24-25 0.020643135308482385 1102 26-27 0.1267323563867464 1102 28-29 0.01611096678980317 1102 30-31 0.14562580961753469 1102 32-33 0.43621140927226776 1102 34-35 0.5698996887940027 1102 36-37 0.46912643435893386 1102 38-39 0.6678468648050782 1102 40-41 0.6821970985619785 1102 42-43 0.7727054361210008 1102 44-45 0.6010443175528906 1102 46-47 1.0579244367227787 1102 48-49 1.371844358768449 1102 50-51 1.319529895183841 1102 52-53 1.4892196487977714 1102 54-55 1.6434425917039732 1102 56-57 1.8241383216341234 1102 58-59 1.3608412962769272 1102 60-61 1.485242582812564 1102 62-63 1.8106551915167195 1102 64-65 1.9698316224495294 1102 66-67 1.9918836533275375 1102 68-69 2.3690387397706765 1102 70-71 2.457257345808795 1102 72-73 2.3928527596516496 1102 74-75 2.552557445081593 1102 76-77 2.266745283886749 1102 78-79 2.272014862812668 1102 80-81 2.5378388267540934 1102 82-83 2.367452000006285 1102 84-85 2.5530433107277872 1102 86-87 2.6039339886724555 1102 88-89 2.915390405536737 1102 90-91 2.6322401585597994 1102 92-93 2.5877972311362107 1102 94-95 2.5986090843162053 1102 96-97 2.6819620742473766 1102 98-99 2.467434072772189 1102 100-101 2.3387800415918854 1103 1 -0.15520273893150716 1103 2 -0.019119485266209324 1103 3 0.05891572961866842 1103 4 -0.12987540191207358 1103 5 -0.5284015884901052 1103 6 -0.2980582412931767 1103 7 -0.09983423263713576 1103 8 -0.13657001834028648 1103 9 -0.2644060854825767 1103 10-11 -0.2370314270380618 1103 12-13 -0.2684853923874613 1103 14-15 -0.2815486550424282 1103 16-17 -0.45375799393700333 1103 18-19 -0.3787453215244909 1103 20-21 -0.25139055500474683 1103 22-23 -0.30637430646451946 1103 24-25 -0.2938524489306431 1103 26-27 -0.31509848600455825 1103 28-29 -0.4961512614710628 1103 30-31 -0.6081955219042001 1103 32-33 -0.24489422931468852 1103 34-35 -0.18020221337990705 1103 36-37 -0.14870644607584893 1103 38-39 -0.24403323030362145 1103 40-41 -0.10965921937280854 1103 42-43 -0.2067782595311769 1103 44-45 -0.20790609005580762 1103 46-47 0.604672059005388 1103 48-49 0.2410274430075816 1103 50-51 0.4836518381186252 1103 52-53 0.9861031406455965 1103 54-55 0.7805264911604937 1103 56-57 0.7462850607645635 1103 58-59 0.5901976142117071 1103 60-61 0.89126330292126 1103 62-63 0.8581246208645474 1103 64-65 0.9674199376669215 1103 66-67 1.1213673489797138 1103 68-69 1.043936837596764 1103 70-71 1.134541652874013 1103 72-73 1.2136747705212123 1103 74-75 1.5226491570381135 1103 76-77 1.2309266697563146 1103 78-79 1.553035582921364 1103 80-81 1.560613962623659 1103 82-83 1.3952967690280254 1103 84-85 1.097863283553874 1103 86-87 1.3080695185637587 1103 88-89 1.1690419001019556 1103 90-91 1.0397979031250166 1103 92-93 1.265837312657947 1103 94-95 1.1596230109466426 1103 96-97 1.332089451964766 1103 98-99 1.152641273859146 1103 100-101 0.9739023242005835 1104 1 0.014405740410907697 1104 2 -0.030108020849162642 1104 3 0.04525576855442637 1104 4 -0.030567593129902093 1104 5 -0.10824151927098313 1104 6 -0.08359793413332284 1104 7 -0.33477907364946446 1104 8 -0.10415600146811954 1104 9 -0.09237493687777487 1104 10-11 -0.07920642163031033 1104 12-13 -0.0994609493850831 1104 14-15 0.040720434293497476 1104 16-17 0.030510879089170828 1104 18-19 0.04021963629510594 1104 20-21 -0.08178207566231777 1104 22-23 -0.11496180376064302 1104 24-25 -0.2945824943556943 1104 26-27 -0.24093127856350094 1104 28-29 -0.24405305684204137 1104 30-31 -0.3909394111536102 1104 32-33 -0.3885563534764884 1104 34-35 -0.09352148657912807 1104 36-37 -0.21042502708385058 1104 38-39 -0.2968074029188088 1104 40-41 -0.4795867556046929 1104 42-43 -0.4476770265655645 1104 44-45 -0.4396603621734769 1104 46-47 -0.5623190722946347 1104 48-49 -0.6332425115673672 1104 50-51 -0.2461718694305901 1104 52-53 -0.12705930798083642 1104 54-55 0.05620786689054569 1104 56-57 -0.2535202604554314 1104 58-59 -0.2805464913474509 1104 60-61 -0.08705705182673995 1104 62-63 -0.1845154397021851 1104 64-65 -0.1502904235703717 1104 66-67 -0.270942839209777 1104 68-69 -0.2334371619706168 1104 70-71 -0.4143659828603603 1104 72-73 -0.2166194107463575 1104 74-75 -0.34283862146069666 1104 76-77 -0.1562083075311591 1104 78-79 -0.08262308028434973 1104 80-81 -0.28734191018620336 1104 82-83 -0.21387476460814625 1104 84-85 -0.15998335282942833 1104 86-87 -0.4745554003200816 1104 88-89 -0.20038812369211811 1104 90-91 -0.25699530474865995 1104 92-93 -0.13102078814239704 1104 94-95 0.11479281410476361 1104 96-97 0.2035581952050869 1104 98-99 0.3588655870756732 1104 100-101 0.6117511236814366 1105 1 -0.10479513023585696 1105 2 -0.0718368765705577 1105 3 -0.13035057472915668 1105 4 0.07039633721836225 1105 5 -0.4183472406640121 1105 6 -0.18352019781491435 1105 7 -0.0073070587240948726 1105 8 -0.06306458355767575 1105 9 0.15067543625654878 1105 10-11 -0.29864827486414214 1105 12-13 -0.20632506630050074 1105 14-15 -0.09058398112938448 1105 16-17 -0.09186940698048573 1105 18-19 -0.053133908481015624 1105 20-21 -0.11348294630909095 1105 22-23 -0.28015148037756177 1105 24-25 -0.18189592719151193 1105 26-27 -0.19397076861325502 1105 28-29 -0.24560778321019683 1105 30-31 -0.10085856538246674 1105 32-33 -0.22648390322773082 1105 34-35 0.08474343879399981 1105 36-37 -0.04102981564106756 1105 38-39 0.2285890523050753 1105 40-41 0.20895491106197994 1105 42-43 -0.039794563878999156 1105 44-45 0.2834661925528934 1105 46-47 0.9327291242227815 1105 48-49 0.904070921268449 1105 50-51 1.5634752076838438 1105 52-53 1.3390243362977685 1105 54-55 1.598325404203976 1105 56-57 1.4600758216341205 1105 58-59 1.4840834837769243 1105 60-61 1.3452035203125625 1105 62-63 1.2626083165167188 1105 64-65 1.6112378724495287 1105 66-67 1.5340711533275382 1105 68-69 1.327632489770675 1105 70-71 1.357843283308796 1105 72-73 1.395196509651651 1105 74-75 1.2998230700815938 1105 76-77 1.674948408886749 1105 78-79 1.513225800312668 1105 80-81 1.3171357017540934 1105 82-83 1.4176473125062863 1105 84-85 1.4581214357277865 1105 86-87 1.2306918011724548 1105 88-89 0.9126560305367342 1105 90-91 0.991224533559798 1105 92-93 1.5663128561362107 1105 94-95 1.4968512718162081 1105 96-97 0.9227823867473752 1105 98-99 1.2264184477721898 1105 100-101 1.723155041591884 1106 1 -0.30086293466390046 1106 2 -0.12160624926428198 1106 3 -0.1088445784191947 1106 4 -0.032394253187547406 1106 5 -0.2134810045016522 1106 6 -0.05901604652340353 1106 7 0.06795816267811716 1106 8 -0.06183072746911478 1106 9 0.07937700452223595 1106 10-11 -0.12326197412613737 1106 12-13 -0.5728091530163724 1106 14-15 -0.20692969146148954 1106 16-17 -0.2759503571649873 1106 18-19 -0.20489244169134935 1106 20-21 -0.6479732599622281 1106 22-23 -0.21217408185358266 1106 24-25 -0.06436825191476458 1106 26-27 -0.2790203534841069 1106 28-29 -0.19325538468621062 1106 30-31 0.14362944199761074 1106 32-33 -0.11877518551555255 1106 34-35 0.05699897384935326 1106 36-37 0.22626146664675417 1106 38-39 -0.1489305510159582 1106 40-41 -0.1330307900450336 1106 42-43 -0.6417894900782599 1106 44-45 -0.43097684250246004 1106 46-47 -0.46748420603552177 1106 48-49 -0.6162242816835786 1106 50-51 -0.11124227386597596 1106 52-53 -0.257302923849835 1106 54-55 -0.27458741867425474 1106 56-57 -0.12149129091200095 1106 58-59 -0.18578505865849948 1106 60-61 -0.061772955702195986 1106 62-63 -0.30160985691501807 1106 64-65 -0.3726931699858973 1106 66-67 0.24540071974820066 1106 68-69 0.5249572130179097 1106 70-71 0.04668668736783488 1106 72-73 0.2744400520870762 1106 74-75 0.20139133576425294 1106 76-77 -0.07020841768151698 1106 78-79 -0.36448407053604015 1106 80-81 -0.35629603256325026 1106 82-83 -0.5964728443202851 1106 84-85 -0.5588469683312525 1106 86-87 -0.6842816766873234 1106 88-89 -0.9812669399429659 1106 90-91 -0.8130939627501625 1106 92-93 -1.2575743671110224 1106 94-95 -1.3153489126856392 1106 96-97 -1.4099609250607408 1106 98-99 -1.4907286924492134 1106 100-101 -1.2509051521350507 1107 1 0.07761908545042218 1107 2 0.09017292735101279 1107 3 0.20721069978064577 1107 4 -0.25178503533066277 1107 5 -0.03709724066401776 1107 6 -0.08787068801099451 1107 7 -0.11496637244958663 1107 8 -0.16312585806748103 1107 9 -0.11862603433168317 1107 10-11 -0.25039459839356226 1107 12-13 -0.18864737022207123 1107 14-15 -0.06432785367839955 1107 16-17 0.17102887733324224 1107 18-19 0.08078153269546107 1107 20-21 -0.38915696591692495 1107 22-23 -0.27555589214227183 1107 24-25 -0.29268024091700795 1107 26-27 -0.48425875880933233 1107 28-29 -0.313499940072937 1107 30-31 0.13896986599008443 1107 32-33 0.17710065559580102 1107 34-35 -0.08670263963737312 1107 36-37 -0.14020260975870968 1107 38-39 0.13983292485409748 1107 40-41 0.14100147968942878 1107 42-43 0.017497102787665142 1107 44-45 0.10488163372936299 1107 46-47 0.6273063301051351 1107 48-49 1.1217179800919794 1107 50-51 1.196716629252471 1107 52-53 0.9069471304154177 1107 54-55 0.7449553061647549 1107 56-57 0.5927044981047089 1107 58-59 0.897870248482814 1107 60-61 1.0622991085478581 1107 62-63 0.9843607674971118 1107 64-65 1.1050797842142366 1107 66-67 1.2877782611706756 1107 68-69 1.2506717054569485 1107 70-71 1.3092219597793822 1107 72-73 1.2123533723967483 1107 74-75 1.4532544426306124 1107 76-77 0.9477425265338084 1107 78-79 0.9382870748224725 1107 80-81 1.2132141331266446 1107 82-83 1.2025431458396163 1107 84-85 0.9766569749434737 1107 86-87 0.8272297913685343 1107 88-89 0.8765653442622288 1107 90-91 1.0022233080696061 1107 92-93 1.0912209443715035 1107 94-95 1.15408166397307 1107 96-97 1.5378865534140438 1107 98-99 0.5272150163996407 1107 100-101 0.7635655808075725 1108 1 -0.13252775355424262 1108 2 -0.1350095223104617 1108 3 0.0022867571094096206 1108 4 -0.08097698116728935 1108 5 -0.0254380478389038 1108 6 0.17832116989809066 1108 7 0.18034708925797105 1108 8 -0.0012512427504987045 1108 9 -0.016911447151521486 1108 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 8.0 6 19.0 7 49.0 8 110.0 9 221.0 10 399.0 11 771.0 12 1238.0 13 1697.0 14 2271.0 15 3463.0 16 5126.0 17 7234.0 18 5844.0 19 3269.0 20 2513.0 21 2500.0 22 2475.0 23 2578.0 24 2776.0 25 2904.0 26 3243.0 27 3548.0 28 4104.0 29 4876.0 30 5878.0 31 7103.0 32 9139.0 33 11541.0 34 16099.0 35 23257.0 36 32741.0 37 47467.0 38 39514.0 39 4015.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.89020059026562 20.813596887830293 16.70982711450922 17.586375407394865 2 15.85196524521814 23.52178712503798 39.867996446058164 20.758251183685715 3 18.4858823122161 29.979653298358034 32.053555288026985 19.480909101398883 4 11.725315681576042 19.538987588127373 37.71986168858392 31.015835041712663 5 11.188044544457908 40.980901275171654 32.558228992364356 15.272825188006077 6 26.823029850803675 39.783378783274934 17.17892404795514 16.214667317966253 7 24.43527325735693 38.56411518771659 20.22862153981069 16.771990015115794 8 27.450739058359265 33.87052728342686 19.12936117510856 19.549372483105316 9 28.428842314985403 16.112741496886454 20.16477366698334 35.293642521144804 10-11 24.39450292892501 29.57387314273846 25.136830606977878 20.89479332135865 12-13 26.909954611893223 28.03581044695746 27.15593507192861 17.89829986922071 14-15 23.12850730596593 27.706707488278532 28.967318350878678 20.19746685487686 16-17 18.615308873700446 31.407576357825018 29.1513617674322 20.825753001042337 18-19 18.621655198409186 27.19900151157916 36.039431830856984 18.13991145915467 20-21 20.505936698295724 25.74338539883766 36.68964164419811 17.0610362586685 22-23 19.717602893974156 26.54790500349054 36.45199691524817 17.282495187287132 24-25 18.90776988434215 26.988049586331837 36.587997276828816 17.516183252497203 26-27 18.502883803582108 27.346227592586537 36.6878664853752 17.463022118456152 28-29 18.169387346913656 27.74728444827374 36.53494061172996 17.54838759308265 30-31 18.370635906627538 27.53076068017739 36.467031551092155 17.63157186210292 32-33 18.213951914089996 27.79344059263134 36.160012000323086 17.83259549295558 34-35 18.394726011854164 28.093640982641837 36.00866177166308 17.50297123384091 36-37 18.438801119263058 28.14446570574151 35.68045232074002 17.736280854255412 38-39 18.536147266069726 28.34644912151143 35.51686205729407 17.600541555124774 40-41 18.430111579927154 28.572692341716895 35.32941271495771 17.667783363398247 42-43 18.268348645909263 29.00260220831899 34.66476132960729 18.064287816164466 44-45 18.537999099975767 29.205209371021528 34.03360859715454 18.223182931848164 46-47 18.94531775855504 29.73945452377564 32.812229560026616 18.502998157642704 48-49 19.135899812687267 30.02330831983938 31.986053470670367 18.854738396802993 50-51 19.146669333405132 30.440242621916745 31.400076155896507 19.013011888781623 52-53 19.237093377693586 30.875121397732624 30.711078204178964 19.17670702039482 54-55 18.913586135011325 30.711403768563 30.76582831076221 19.60918178566346 56-57 19.331097375698576 30.6604408580231 30.348317070074966 19.66014469620336 58-59 19.127053420669018 30.245237371775396 30.789290480897563 19.838418726658023 60-61 19.275326643409628 30.043501171185376 31.36988303531249 19.311289150092502 62-63 19.383021850588285 29.873881219878996 31.507194424465272 19.235902505067447 64-65 19.287057728477304 29.876765912928427 31.38815275795887 19.448023600635402 66-67 19.29686568484536 30.00484628432304 31.031797009919497 19.6664910209121 68-69 19.24763359013512 29.570411511079143 31.195647575127023 19.986307323658714 70-71 19.216671218071255 29.9661913974607 30.886023854488393 19.93111352997965 72-73 19.05897466470251 29.68349147861673 30.74101995053713 20.516513906143626 74-75 18.89127784209575 29.857534625932235 30.452358332724344 20.798829199247674 76-77 18.514344616970458 29.55156484982288 31.258149257864638 20.675941275342026 78-79 18.495305642844233 29.557911174531622 30.948140911486078 20.998642271138067 80-81 18.284723050235968 29.953498748043216 30.569669183401093 21.192109018319723 82-83 18.117638008788713 29.805282844697444 30.297220112118616 21.779859034395223 84-85 18.055870734981326 29.46463943260011 30.397741477655167 22.0817483547634 86-87 17.996638371033065 29.27501894281769 29.97696091817857 22.751381767970678 88-89 17.94471389614336 28.80442935002096 30.157158077332852 23.09369867650283 90-91 18.017747723837108 29.362982109955883 29.93187858925982 22.687391576947192 92-93 17.969744641078208 29.507244592998884 29.388584813856077 23.134425952066834 94-95 17.740285315373875 29.631951629466947 28.822506759797378 23.805256295361797 96-97 18.246837414853477 29.62291292457874 28.353455669960343 23.77679399060744 98-99 18.54952392491264 29.69800819645333 27.8671720697487 23.885295808885324 100-101 18.39242062019052 29.351825317298058 27.439862906467518 24.815891156043907 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 957.0 1 661.0 2 387.0 3 447.5 4 448.5 5 467.0 6 693.0 7 1290.5 8 1942.0 9 2248.0 10 2046.5 11 1649.5 12 1581.5 13 1655.5 14 1646.0 15 1582.5 16 1569.0 17 1553.0 18 1549.5 19 1586.0 20 1629.0 21 1757.0 22 1938.0 23 2103.0 24 2383.0 25 2644.0 26 2970.0 27 3392.0 28 3653.0 29 4112.0 30 4593.5 31 4996.0 32 5372.0 33 5591.0 34 5942.5 35 6309.0 36 6931.0 37 7516.0 38 7673.5 39 7485.5 40 7281.0 41 7441.0 42 7455.5 43 7440.0 44 7659.0 45 7755.0 46 7653.0 47 7877.0 48 8593.5 49 8263.5 50 7492.0 51 7113.5 52 6454.0 53 6113.5 54 5802.0 55 5292.0 56 4789.0 57 4428.0 58 4200.0 59 3695.5 60 2979.5 61 2460.0 62 2060.0 63 1478.0 64 954.5 65 685.5 66 508.0 67 387.5 68 279.0 69 191.5 70 162.0 71 138.0 72 100.0 73 82.5 74 63.5 75 50.0 76 43.5 77 31.0 78 21.0 79 15.0 80 11.5 81 8.5 82 6.5 83 2.5 84 0.5 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04230883139161439 2 0.0 3 0.0 4 0.0 5 0.010769520717865482 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005000134619008973 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0017308158296569524 24-25 7.692514798475343E-4 26-27 0.0025000673095044866 28-29 0.003461631659313905 30-31 7.692514798475343E-4 32-33 0.0 34-35 0.0 36-37 1.9231286996188358E-4 38-39 3.8462573992376715E-4 40-41 0.0 42-43 0.008269453408360994 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.9231286996188358E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.9231286996188358E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.006154011838780274 92-93 0.002307754439542603 94-95 0.0 96-97 0.0 98-99 9.61564349809418E-4 100-101 0.009423330628132295 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 259993.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.80240274504426 #Duplication Level Percentage of deduplicated Percentage of total 1 81.06232948071315 50.09846734018508 2 11.170506312956224 13.8072826003876 3 3.522639903393141 6.531228301058 4 1.4966357671052355 3.6998274576510415 5 0.7746629903570103 2.3938017060862142 6 0.4593664393865686 1.7033969816715406 7 0.3161529481344857 1.36773082807484 8 0.20938411908381643 1.0352353324827464 9 0.1655155791108473 0.9206334432709037 >10 0.7572601005873837 8.313160778357782 >50 0.04120056862247109 1.7635015897874313 >100 0.01997603327150113 2.2268499619892923 >500 0.001872753119203231 0.8329468239665423 >1k 0.0024970041589376414 5.305936855030966 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4799 1.8458189258941586 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4153 1.5973506979034051 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2978 1.1454154534929788 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1823 0.7011727238810276 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 838 0.32231637005611685 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 789 0.3034697087998523 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 532 0.20462089363944413 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 404 0.15538879892920196 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 382 0.14692703265087906 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 372 0.1430807752516414 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 260 0.10000269238017946 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.9231286996188358E-4 0.0 22-23 0.0 0.0 0.0 0.0011538772197713014 0.0 24-25 0.0 0.0 0.0 0.0015385029596950686 0.0 26-27 0.0 0.0 0.0 0.0015385029596950686 0.0 28-29 0.0 0.0 0.0 0.0034616316593139044 0.0 30-31 0.0 0.0 0.0 0.009807956368056063 0.0 32-33 0.0 0.0 0.0 0.01769278403649329 0.0 34-35 0.0 0.0 0.0 0.02480836022508298 0.0 36-37 0.0 0.0 0.0 0.03173162354371079 0.0 38-39 0.0 0.0 0.0 0.04019338982203367 0.0 40-41 0.0 0.0 0.0 0.05865542533837449 0.0 42-43 0.0 0.0 0.0 0.08634847861288572 0.0 44-45 0.0 0.0 0.0 0.13211894166381402 0.0 46-47 0.0 0.0 0.0 0.19635144023108314 0.0 48-49 0.0 0.0 0.0 0.3111622235983277 0.0 50-51 0.0 0.0 0.0 0.45347374737012147 0.0 52-53 0.0 0.0 0.0 0.6032854730704288 0.0 54-55 0.0 0.0 0.0 0.7869442638840276 0.0 56-57 0.0 0.0 0.0 1.0213736523675636 0.0 58-59 0.0 0.0 0.0 1.3040735712115326 0.0 60-61 0.0 0.0 0.0 1.6423519094744858 0.0 62-63 0.0 0.0 0.0 2.0850561361267417 0.0 64-65 1.9231286996188358E-4 0.0 0.0 2.4692972503105857 0.0 66-67 3.8462573992376715E-4 0.0 0.0 2.9077705938236798 0.0 68-69 3.8462573992376715E-4 0.0 0.0 3.5064405580150235 0.0 70-71 3.8462573992376715E-4 0.0 0.0 4.129726569561488 0.0 72-73 3.8462573992376715E-4 0.0 0.0 4.857438469497255 0.0 74-75 3.8462573992376715E-4 0.0 0.0 5.683422245983546 0.0 76-77 3.8462573992376715E-4 0.0 0.0 6.4694049455177645 0.0 78-79 3.8462573992376715E-4 0.0 0.0 7.304619739762224 0.0 80-81 3.8462573992376715E-4 0.0 0.0 8.289261633967069 0.0 82-83 3.8462573992376715E-4 0.0 0.0 9.482178366340632 0.0 84-85 3.8462573992376715E-4 0.0 0.0 10.655863811718007 0.0 86-87 3.8462573992376715E-4 0.0 0.0 11.776663217855866 0.0 88-89 3.8462573992376715E-4 0.0 0.0 13.209009473331975 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACGAGT 25 0.0050176815 56.99781 6 GGTATCA 1990 0.0 49.66544 1 GGGATCG 45 9.604033E-4 42.22872 9 GTATCAA 3550 0.0 40.957844 1 CGGTATC 50 0.0016138211 37.99854 2 TAATGTG 55 0.0025764564 34.544125 5 CCCCTAT 60 0.003938694 31.677635 1 TATCAAC 4705 0.0 30.891478 2 ATCAACG 4735 0.0 30.595444 3 TCAACGC 4805 0.0 30.149725 4 TTAGGCA 80 4.4851945E-4 29.68636 4 CAACGCA 4890 0.0 29.528515 5 GCGGTAT 65 0.005825995 29.240892 1 GTGTAGG 65 0.005825995 29.240892 1 AACGCAG 4970 0.0 29.05321 6 GTAAGTA 100 4.9398714E-5 28.498905 2 TACAGTC 85 6.3879933E-4 27.945477 7 CGCCTTA 35 0.008381124 27.147034 38-39 TACTTGC 35 0.008385058 27.144423 92-93 CTATACT 105 6.887439E-5 27.141815 4 >>END_MODULE