FastQCFastQC Report
Thu 26 May 2016
SRR937181_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937181_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291352
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62322.1389933825750296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46071.5812487987039732No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33891.1631977813778522No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11980.4111864686015541No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.3274389741618386No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9030.3099343749141931No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5670.1946099563414701No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.1815673137647931No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4630.15891430297372253No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4410.15136329937669896No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.13660451961888026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20200.053.8945961
GTATCAA35900.039.462351
TATATAG600.00393645331.6825623
TATCAAC46400.030.3169352
ATCAACG46350.030.1445733
TCAACGC46750.029.8866524
GTACTCA804.4813342E-429.6921776
CAACGCA47400.029.3765535
TACATGG25650.029.0886692
GTACATG26300.028.9217991
AACGCAG48350.028.8876956
ACATGGG26300.027.646953
CTATACT1056.8648864E-527.156484
CATGGGT2355.456968E-1226.2897854
ACGCAGA53250.026.0510547
CGCAGAG53400.025.9778778
GACTCCC1309.975287E-625.5809549
CATGGGA19200.025.2470424
ATGGGAC3200.025.2470425
GAGTACT35900.025.20940412-13
Produced by FastQC (version 0.11.5)